FastQCFastQC Report
Fri 27 May 2016
SRR2138822_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138822_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13853944
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124750.041.7267951
GTATCAA210550.032.4257771
TCAACGC241550.027.348154
ATCAACG245400.026.868933
CAACGCA249450.026.4321215
AACGCAG257900.025.5819636
TATCAAC264900.025.3554862
GTACATG371500.021.9604151
TACATGG375750.021.363242
ACGCAGA311550.021.2163247
CGCAGAG314550.020.9879368
ACATGGG375900.020.7876363
CATGGGG281950.019.1444974
GCAGAGT394650.016.7073739
AGAGTAC340800.016.09669510-11
GAGTACT202000.015.9763312-13
GAGTACA268100.015.5663721
CAGAGTA378950.015.50287310-11
ATGGGGG179600.015.323315
TATACTG73400.014.3950545
Produced by FastQC (version 0.11.5)