##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138822_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13853944
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.22657872732848	33.0	31.0	34.0	30.0	34.0
2	32.44919894291474	34.0	31.0	34.0	31.0	34.0
3	32.55033960004458	34.0	31.0	34.0	31.0	34.0
4	36.078608806272065	37.0	35.0	37.0	35.0	37.0
5	35.8887806244922	37.0	35.0	37.0	35.0	37.0
6	35.92317278025666	37.0	35.0	37.0	35.0	37.0
7	35.90994412854563	37.0	35.0	37.0	35.0	37.0
8	35.86389868473555	37.0	35.0	37.0	35.0	37.0
9	37.69019638017881	39.0	37.0	39.0	35.0	39.0
10-11	37.551299471110895	39.0	37.0	39.0	35.0	39.0
12-13	37.57251404365428	39.0	37.0	39.0	35.0	39.0
14-15	38.86227499548143	40.0	38.0	41.0	36.0	41.0
16-17	38.82052955461636	40.0	38.0	41.0	35.5	41.0
18-19	38.84643531834689	40.0	38.0	41.0	35.0	41.0
20-21	38.842954481164796	40.0	38.0	41.0	35.0	41.0
22-23	38.805227316561286	40.0	38.0	41.0	35.0	41.0
24-25	38.804779495320936	40.0	38.0	41.0	35.0	41.0
26-27	38.66884394948917	40.0	38.0	41.0	35.0	41.0
28-29	38.57870462074023	40.0	38.0	41.0	34.5	41.0
30-31	38.47688288688516	40.0	38.0	41.0	34.0	41.0
32-33	38.39603756822481	40.0	38.0	41.0	34.0	41.0
34-35	38.327029641815045	40.0	38.0	41.0	34.0	41.0
36-37	38.29630415526715	40.0	38.0	41.0	34.0	41.0
38-39	38.22620152535121	40.0	38.0	41.0	34.0	41.0
40-41	38.19769175954329	40.0	38.0	41.0	34.0	41.0
42-43	38.16565542814337	40.0	38.0	41.0	34.0	41.0
44-45	38.096790927531586	40.0	38.0	41.0	33.0	41.0
46-47	38.012207232056724	40.0	37.0	41.0	33.0	41.0
48-49	37.97743284537691	40.0	37.0	41.0	33.0	41.0
50-51	37.899113776863835	40.0	37.0	41.0	33.0	41.0
52-53	37.71726047415926	39.5	37.0	41.0	33.0	41.0
54-55	37.55905166764508	39.0	36.0	40.5	32.5	41.0
56-57	37.405503119988055	39.0	36.0	40.0	32.0	41.0
58-59	37.23449811007836	39.0	35.5	40.0	32.0	41.0
60-61	37.09696662396405	39.0	35.0	40.0	32.0	41.0
62-63	36.886616557780776	38.5	35.0	40.0	31.5	41.0
64-65	36.656049424020324	38.0	35.0	40.0	31.0	41.0
66-67	36.42023768393159	37.5	35.0	40.0	31.0	41.0
68-69	36.14381573167472	37.0	35.0	39.5	31.0	41.0
70-71	35.83880373015123	36.5	34.0	39.0	31.0	41.0
72-73	35.52992795553468	36.0	34.0	39.0	30.5	40.0
74-75	35.134679503607344	35.5	34.0	38.5	30.0	40.0
76-77	33.124107058844054	34.0	31.0	36.0	27.5	39.0
78-79	34.312814115860036	35.0	33.5	37.0	29.5	39.0
80-81	34.3265285904751	35.0	34.0	36.5	30.0	39.0
82-83	34.07651890966046	35.0	34.0	36.0	30.0	38.0
84-85	33.785489355387895	35.0	33.0	36.0	29.0	37.0
86-87	33.61676423847979	35.0	33.0	35.0	29.0	37.0
88-89	33.4432477375188	35.0	33.0	35.0	29.0	36.0
90-91	33.28255151140154	35.0	33.0	35.0	29.0	36.0
92-93	33.21702699407463	35.0	33.0	35.0	29.0	36.0
94-95	33.16244493737965	35.0	33.0	35.0	29.0	35.5
96-97	33.209946692360404	35.0	33.0	35.0	29.0	35.0
98-99	33.27999507360886	34.5	33.0	35.0	29.5	35.0
100-101	32.02652155438313	33.0	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	7.0
19	18.0
20	75.0
21	356.0
22	1371.0
23	4613.0
24	11828.0
25	25026.0
26	45987.0
27	74985.0
28	112286.0
29	155897.0
30	210406.0
31	278645.0
32	370590.0
33	509960.0
34	748452.0
35	1161509.0
36	2024694.0
37	3364486.0
38	3451893.0
39	1284476.0
40	16383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.036686448277834	20.203459751244843	13.3556913468107	24.404162453666622
2	15.16466592467888	21.50053375335838	40.39584295315278	22.938957368809966
3	18.7701855875843	25.876645668554744	30.10685621365295	25.246312530208005
4	12.84246827488297	17.67953264732607	38.48519225055675	30.992806827234215
5	13.321384349733195	37.754002519724054	35.68756605541631	13.237047075126437
6	30.544641856263794	37.64974220603552	18.659881377747034	13.145734559953654
7	26.817294771799276	32.17333634378773	23.322131228479055	17.687237655933934
8	24.5857786057169	36.7677319902549	21.347314526462643	17.29917487756555
9	25.73283824447392	16.365772808089886	21.098973692978692	36.80241525445751
10-11	24.501620029720847	26.523388248626517	28.989023858783575	19.985967862869064
12-13	25.033988685476082	24.005749293970098	29.152738854652576	21.807523165901237
14-15	22.12118638736755	25.4717060463772	26.3811043507802	26.026003215475047
16-17	22.06815979622842	28.583116836620675	27.108894044901582	22.239829322249317
18-19	22.119843543242588	28.003065008319744	28.428316686632314	21.448774761805357
20-21	22.818127216996068	27.28802945108097	28.198968256440292	21.69487507548267
22-23	22.562654287231343	27.180891863668833	28.050051238069866	22.206402611029954
24-25	22.32766127472517	27.260082419850807	28.1552248345987	22.25703147082532
26-27	22.30365722510314	27.43065647294441	28.07432736873566	22.191358933216794
28-29	22.251529846035336	27.54223104454952	28.022679127338513	22.18355998207663
30-31	22.485891662670113	27.410592581850622	27.943728504372313	22.15978725110695
32-33	22.267227995194073	27.52435177900376	27.942910553587357	22.265509672214815
34-35	22.388506919980887	27.521047686981458	27.942968113274958	22.147477279762697
36-37	22.406474946616726	27.50617694275594	27.858604548605147	22.22874356202219
38-39	22.289218426530553	27.607360236386548	27.909839407866272	22.19358192921662
40-41	22.47030138232845	27.480799705372178	27.819905227451947	22.228993684847424
42-43	22.374919293506846	27.557307655570078	27.681662154760595	22.38611089616248
44-45	22.472305383271543	27.599566247865177	27.555375475195078	22.372752893668206
46-47	22.490963754327467	27.60562068436498	27.431645203522585	22.471770357784965
48-49	22.434278849869237	27.67437404362889	27.40455226668211	22.486794839819762
50-51	22.415450360881238	27.748171179496676	27.389241101171997	22.44713735845009
52-53	22.588017748092845	27.622146255254098	27.274864989236974	22.514971007416086
54-55	22.551900527725792	27.528184343420897	27.323366538687203	22.596548590166105
56-57	22.532505281256604	27.63586345982964	27.294762493499025	22.536868765414727
58-59	22.514603685058475	27.677096144086523	27.246116717518255	22.562183453336747
60-61	22.602129602705332	27.608541781642966	27.3015249461114	22.487803669540302
62-63	22.57027772898622	27.65874564414861	27.232243873111333	22.53873275375384
64-65	22.559529609090063	27.619393465310267	27.193110162388145	22.627966763211525
66-67	22.608110420731396	27.637760770676934	27.11897198518573	22.635156823405943
68-69	22.54693825774932	27.624964590658195	27.16777317468105	22.660323976911435
70-71	22.551852016630374	27.47506161304451	27.211560138282604	22.761526232042513
72-73	22.586251076619	27.503815543708495	27.18155183641277	22.728381543259733
74-75	22.5024042616874	27.58792577150182	27.157237387513444	22.752432579297338
76-77	22.66261900837881	27.515568310018452	27.080255364201882	22.741557317400854
78-79	22.579976682117213	27.543064367293884	27.084559834834298	22.792399115754606
80-81	22.599769185696907	27.563361776555833	27.07653316877831	22.760335868968944
82-83	22.585788832855965	27.57154493087979	27.124233135655935	22.718433100608312
84-85	22.722065252516142	27.499003738357985	27.10322334226854	22.67570766685733
86-87	22.564009998340676	27.518627674095832	27.16920642310295	22.74815590446055
88-89	22.62462589480038	27.48437163405028	27.141350684677068	22.74965178647227
90-91	22.655214643715524	27.50087645722546	27.12292701391419	22.720981885144827
92-93	22.630499706848443	27.478006113966234	27.14502827209878	22.74646590708653
94-95	22.560217548300248	27.501729860436747	27.193515619796177	22.744536971466832
96-97	22.572701567604778	27.521828969408052	27.233819991165266	22.671649471821905
98-99	22.804299270541154	28.086579568902952	27.277553111835402	21.83156804872049
100-101	27.10123067809554	35.09735499402238	27.82175082743902	9.979663500443053
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1838.0
1	1696.5
2	2503.0
3	3668.0
4	4952.0
5	8150.5
6	10906.5
7	12658.0
8	13711.5
9	13272.0
10	11961.0
11	10729.5
12	11428.0
13	14839.5
14	22796.0
15	33723.5
16	42723.0
17	48649.0
18	51784.5
19	52212.0
20	51576.5
21	51823.5
22	54687.0
23	62492.5
24	75876.5
25	94602.5
26	118876.5
27	145426.0
28	175088.5
29	207477.5
30	237798.5
31	268741.5
32	301017.5
33	332719.5
34	365491.0
35	396786.0
36	429448.0
37	460312.0
38	486648.0
39	511614.0
40	535665.0
41	560167.0
42	579105.0
43	593347.5
44	607103.5
45	618730.0
46	625440.0
47	625159.0
48	620490.0
49	612483.0
50	602027.5
51	587412.5
52	563262.5
53	536127.5
54	508501.0
55	475954.0
56	440104.0
57	400091.5
58	356922.0
59	311137.5
60	264089.0
61	220332.5
62	180097.0
63	142751.0
64	109013.0
65	82770.0
66	63577.5
67	49526.0
68	38587.5
69	28716.0
70	20965.5
71	15344.5
72	11144.5
73	7915.5
74	5540.5
75	3854.0
76	2663.5
77	1809.0
78	1206.5
79	826.5
80	599.0
81	428.5
82	310.5
83	211.0
84	134.0
85	93.0
86	69.5
87	48.5
88	31.5
89	19.5
90	11.5
91	9.0
92	6.0
93	3.5
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0012054329077698018
3	0.0
4	0.001818976603341258
5	3.3203541172102327E-4
6	3.6812621734287366E-4
7	0.0
8	0.0
9	0.0
10-11	2.2737207541765724E-4
12-13	4.980531175815349E-4
14-15	2.5263563935295246E-5
16-17	0.0
18-19	0.0010682878464067706
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.4177070169057745E-5
28-29	0.0
30-31	5.0692405759830454E-5
32-33	8.936652754913932E-4
34-35	7.368651596582522E-4
36-37	4.3673864572190454E-4
38-39	0.0016221239335275834
40-41	7.480900885993015E-6
42-43	1.5113573212944655E-5
44-45	1.1465216882348416E-5
46-47	1.5484787067725233E-5
48-49	1.5683434885534646E-5
50-51	1.190941886561515E-5
52-53	8.032409809692934E-6
54-55	0.002999206429485792
56-57	0.0027703240418805487
58-59	0.0014891920323971432
60-61	1.606530020296393E-4
62-63	8.566183986469884E-6
64-65	9.109906993489063E-5
66-67	3.5176580277089444E-5
68-69	9.812687832128818E-5
70-71	0.0
72-73	0.0
74-75	1.257006999294307E-4
76-77	4.722320249720073E-6
78-79	3.3539767407462704E-4
80-81	0.0
82-83	1.5319719834940395E-4
84-85	1.0034108443125294E-5
86-87	0.001319495376060258
88-89	0.0
90-91	9.489452684218217E-5
92-93	1.6113127907781775E-5
94-95	1.698086842242638E-4
96-97	1.6817385005384085E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	405.0
20-21	1112.0
22-23	2203.0
24-25	5098.0
26-27	8246.0
28-29	18208.0
30-31	42855.0
32-33	53119.0
34-35	72747.0
36-37	115106.0
38-39	134748.0
40-41	132774.0
42-43	144206.0
44-45	165106.0
46-47	165747.0
48-49	159557.0
50-51	147901.0
52-53	143751.0
54-55	154752.0
56-57	160021.0
58-59	160021.0
60-61	155644.0
62-63	147375.0
64-65	152131.0
66-67	160723.0
68-69	162582.0
70-71	160061.0
72-73	150559.0
74-75	152187.0
76-77	150140.0
78-79	158972.0
80-81	159923.0
82-83	152485.0
84-85	150427.0
86-87	158503.0
88-89	167437.0
90-91	174067.0
92-93	178262.0
94-95	195753.0
96-97	294305.0
98-99	1115179.0
100-101	7569546.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.41822811905699
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.54541690017554	75.5926543089508
2	7.118197526983515	12.018112452588035
3	1.8334636353343818	4.643332542469601
4	0.6872417017424403	2.32062907002489
5	0.3058633680218026	1.2910221787463807
6	0.17443739376777118	0.8835417419748887
7	0.09733363409472046	0.5751713048665454
8	0.05554375881501378	0.3751124561788572
9	0.04568988523481048	0.3471353239037189
>10	0.1334795299937917	1.7188322558097313
>50	0.002751924479330324	0.15547799723404365
>100	5.658734079837798E-4	0.07198731602762742
>500	1.4867948796239814E-5	0.0069910512248089135
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.330896674622043E-5	0.0	7.218161124370071E-6	0.0
3	0.0	5.05271278705905E-5	0.0	7.218161124370071E-6	0.0
4	7.218161124370071E-6	1.01054255741181E-4	0.0	1.4436322248740142E-5	0.0
5	7.218161124370071E-6	1.01054255741181E-4	0.0	2.1654483373110215E-5	0.0
6	7.218161124370071E-6	1.01054255741181E-4	0.0	2.1654483373110215E-5	7.218161124370071E-6
7	7.218161124370071E-6	1.01054255741181E-4	0.0	5.05271278705905E-5	7.218161124370071E-6
8	7.218161124370071E-6	1.3714506136303136E-4	0.0	5.05271278705905E-5	7.218161124370071E-6
9	7.218161124370071E-6	2.3098115597984227E-4	0.0	1.1549057798992114E-4	2.1654483373110215E-5
10-11	2.8872644497480284E-5	2.3098115597984227E-4	0.0	1.4075414192521639E-4	2.1654483373110215E-5
12-13	5.05271278705905E-5	2.3098115597984227E-4	0.0	1.8045402810925177E-4	2.8872644497480284E-5
14-15	7.579069180588575E-5	2.851173644126178E-4	0.0	1.8767218923362183E-4	3.609080562185036E-5
16-17	9.744517517899597E-5	2.887264449748028E-4	0.0	2.2376299485547222E-4	5.05271278705905E-5
18-19	1.1549057798992114E-4	2.995536866613579E-4	0.0	2.5985380047732255E-4	5.4136208432775533E-5
20-21	1.1549057798992114E-4	3.248172505966532E-4	0.0	3.212081700344682E-4	6.496345011933064E-5
22-23	1.2992690023866127E-4	3.6451713678068863E-4	0.0	3.8256253959161377E-4	6.496345011933064E-5
24-25	1.840631086714368E-4	4.3308966746220427E-4	0.0	4.6196231195968454E-4	6.496345011933064E-5
26-27	2.0932667260673205E-4	4.763986342084247E-4	0.0	6.06325534447086E-4	6.496345011933064E-5
28-29	2.1293575316891708E-4	4.8000771477060974E-4	0.0	0.0012848326801378727	6.496345011933064E-5
30-31	2.1654483373110213E-4	5.702347288252356E-4	0.0	0.0036920894151152915	6.496345011933064E-5
32-33	2.2376299485547222E-4	5.810619705117906E-4	0.0	0.0081998310372844	6.496345011933064E-5
34-35	2.3459023654202733E-4	6.676799040042315E-4	0.0	0.0142053410927603	7.21816112437007E-5
36-37	2.4541747822858244E-4	7.506887569344873E-4	0.0	0.022596453399840507	7.21816112437007E-5
38-39	2.4541747822858244E-4	8.228703681781881E-4	0.0	0.03569741584057219	7.21816112437007E-5
40-41	2.526356393529525E-4	9.527972684168494E-4	0.0	0.049058232081781186	7.21816112437007E-5
42-43	2.526356393529525E-4	9.816699129143298E-4	0.0	0.059997355265764024	7.579069180588575E-5
44-45	2.526356393529525E-4	0.0010971604909042506	0.0	0.07201920261840239	9.022701405462589E-5
46-47	2.526356393529525E-4	0.0011982147466454319	0.0	0.08523565563712399	1.01054255741181E-4
48-49	2.526356393529525E-4	0.0012054329077698018	0.0	0.09699764918928502	1.0827241686555107E-4
50-51	2.5985380047732255E-4	0.0013064871635109828	0.0	0.10811722640137711	1.0827241686555107E-4
52-53	2.7789920328824774E-4	0.001313705324635353	0.0	0.11883258659050448	1.1188149742773611E-4
54-55	2.8150828385043277E-4	0.001328141646884093	0.0	0.12939636539602006	1.1909965855210618E-4
56-57	2.8150828385043277E-4	0.0013425779691328332	0.0	0.13977969017342642	1.2270873911429122E-4
58-59	2.8150828385043277E-4	0.0013461870496950183	0.0	0.14925713572972432	1.2270873911429122E-4
60-61	2.8150828385043277E-4	0.001403932338689979	0.0	0.1589547352003155	1.2270873911429122E-4
62-63	2.8150828385043277E-4	0.0014544594665605692	0.0	0.16789081867228567	1.2270873911429122E-4
64-65	2.8150828385043277E-4	0.0015879954473614157	0.0	0.17667171168008186	1.2270873911429122E-4
66-67	2.887264449748028E-4	0.001595213608485786	0.0	0.18542734112394277	1.2631781967647625E-4
68-69	3.03162767223543E-4	0.001638522575232006	0.0	0.1942731975818583	1.3714506136303136E-4
70-71	3.03162767223543E-4	0.0016565679780429312	0.0	0.20236114712171493	1.443632224874014E-4
72-73	3.03162767223543E-4	0.0016782224614160417	0.0	0.20810680337671353	1.515813836117715E-4
74-75	3.03162767223543E-4	0.001721531428162262	0.0	0.21278056270474316	1.515813836117715E-4
76-77	3.03162767223543E-4	0.0017864948782815927	0.0	0.21700318696249965	1.515813836117715E-4
78-79	3.03162767223543E-4	0.001826194764465628	0.0	0.22090099396965945	1.515813836117715E-4
80-81	3.03162767223543E-4	0.001826194764465628	0.0	0.22289320643998561	1.5879954473614155E-4
82-83	3.03162767223543E-4	0.001829803845027813	0.0	0.22351035921611925	1.6601770586051164E-4
84-85	3.03162767223543E-4	0.0018370220061521832	0.0	0.22415638463675036	1.6601770586051164E-4
86-87	3.03162767223543E-4	0.0018550674089631083	0.0	0.2245136836124067	1.6601770586051164E-4
88-89	3.03162767223543E-4	0.001941685342455549	0.0	0.22464361051264536	1.6601770586051164E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12475	0.0	41.726795	1
GTATCAA	21055	0.0	32.425777	1
TCAACGC	24155	0.0	27.34815	4
ATCAACG	24540	0.0	26.86893	3
CAACGCA	24945	0.0	26.432121	5
AACGCAG	25790	0.0	25.581963	6
TATCAAC	26490	0.0	25.355486	2
GTACATG	37150	0.0	21.960415	1
TACATGG	37575	0.0	21.36324	2
ACGCAGA	31155	0.0	21.216324	7
CGCAGAG	31455	0.0	20.987936	8
ACATGGG	37590	0.0	20.787636	3
CATGGGG	28195	0.0	19.144497	4
GCAGAGT	39465	0.0	16.707373	9
AGAGTAC	34080	0.0	16.096695	10-11
GAGTACT	20200	0.0	15.97633	12-13
GAGTACA	26810	0.0	15.566372	1
CAGAGTA	37895	0.0	15.502873	10-11
ATGGGGG	17960	0.0	15.32331	5
TATACTG	7340	0.0	14.395054	5
>>END_MODULE