FastQCFastQC Report
Fri 27 May 2016
SRR2138821_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138821_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10568093
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104900.044.2883571
GTATCAA171400.033.887931
TCAACGC198050.028.3017644
ATCAACG201450.027.759743
CAACGCA202500.027.6147545
AACGCAG209950.026.7376446
TATCAAC218650.026.0706542
GTACATG292450.023.2895551
TACATGG285650.023.2690532
ACGCAGA245050.022.855167
ACATGGG287000.022.4514373
CGCAGAG252500.022.180828
CATGGGG198550.020.742994
GCAGAGT317150.017.6593329
AGAGTAC274950.017.37556810-11
GAGTACA198300.017.1299631
GAGTACT175400.016.93882612-13
CAGAGTA305500.016.59991610-11
ATGGGGG128050.015.7942225
AGTACAT207000.015.512262
Produced by FastQC (version 0.11.5)