##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138821_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10568093
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36701096404053	33.0	31.0	34.0	31.0	34.0
2	32.58055403183904	34.0	31.0	34.0	31.0	34.0
3	32.68116745376862	34.0	31.0	34.0	31.0	34.0
4	36.172391745606326	37.0	35.0	37.0	35.0	37.0
5	36.006776719319184	37.0	35.0	37.0	35.0	37.0
6	36.04425197620801	37.0	35.0	37.0	35.0	37.0
7	36.02910014134054	37.0	35.0	37.0	35.0	37.0
8	35.984824319770844	37.0	35.0	37.0	35.0	37.0
9	37.82297941549152	39.0	38.0	39.0	35.0	39.0
10-11	37.694483716220134	39.0	37.0	39.0	35.0	39.0
12-13	37.71301615154219	39.0	37.0	39.0	35.0	39.0
14-15	39.048466360014054	40.0	38.0	41.0	36.0	41.0
16-17	39.014693757899366	40.0	38.0	41.0	36.0	41.0
18-19	39.04506839597267	40.0	38.5	41.0	36.0	41.0
20-21	39.03874782445321	40.0	39.0	41.0	36.0	41.0
22-23	39.00020808841238	40.0	39.0	41.0	36.0	41.0
24-25	39.003783288906625	40.0	38.0	41.0	36.0	41.0
26-27	38.87749404108243	40.0	38.0	41.0	35.0	41.0
28-29	38.790634801572224	40.0	38.0	41.0	35.0	41.0
30-31	38.68929218411013	40.0	38.0	41.0	35.0	41.0
32-33	38.606500227262856	40.0	38.0	41.0	35.0	41.0
34-35	38.541219844124015	40.0	38.0	41.0	35.0	41.0
36-37	38.5078114450144	40.0	38.0	41.0	34.5	41.0
38-39	38.44104975431526	40.0	38.0	41.0	34.0	41.0
40-41	38.41141341533054	40.0	38.0	41.0	34.0	41.0
42-43	38.37726183426377	40.0	38.0	41.0	34.0	41.0
44-45	38.30630586254996	40.0	38.0	41.0	34.0	41.0
46-47	38.22392117938294	40.0	38.0	41.0	34.0	41.0
48-49	38.18258606610857	40.0	38.0	41.0	33.5	41.0
50-51	38.101772383560686	40.0	37.0	41.0	33.0	41.0
52-53	37.92523489464723	40.0	37.0	41.0	33.0	41.0
54-55	37.775800450009974	39.5	37.0	41.0	33.0	41.0
56-57	37.62672458416711	39.0	36.0	41.0	33.0	41.0
58-59	37.45535802255575	39.0	36.0	40.5	32.5	41.0
60-61	37.31606387293011	39.0	36.0	40.0	32.0	41.0
62-63	37.11047504008259	39.0	35.0	40.0	32.0	41.0
64-65	36.879833635860564	38.0	35.0	40.0	32.0	41.0
66-67	36.64781746859684	38.0	35.0	40.0	31.0	41.0
68-69	36.37121258597296	37.0	35.0	40.0	31.0	41.0
70-71	36.06976038751283	37.0	35.0	39.0	31.0	41.0
72-73	35.76225379370047	36.0	34.0	39.0	31.0	40.5
74-75	35.36916086387532	36.0	34.0	39.0	30.5	40.0
76-77	33.3778162724424	34.5	32.0	36.5	27.5	39.0
78-79	34.540952596373	35.0	33.5	37.0	30.0	39.0
80-81	34.54078168331097	35.0	34.0	37.0	30.0	39.0
82-83	34.282614873748564	35.0	34.0	36.0	30.0	38.5
84-85	33.981123756496345	35.0	34.0	36.0	30.0	37.0
86-87	33.80490688355546	35.0	34.0	35.5	30.0	37.0
88-89	33.62279383423856	35.0	33.0	35.0	30.0	36.5
90-91	33.456139496477796	35.0	33.0	35.0	29.0	36.0
92-93	33.379650855468626	35.0	33.0	35.0	29.0	36.0
94-95	33.316236042060524	35.0	33.0	35.0	29.0	36.0
96-97	33.352479545647086	35.0	33.0	35.0	30.0	35.5
98-99	33.41126155828964	35.0	33.0	35.0	30.0	35.0
100-101	32.138233565614115	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	7.0
20	52.0
21	200.0
22	897.0
23	3004.0
24	8168.0
25	17727.0
26	33000.0
27	54667.0
28	81065.0
29	113763.0
30	152626.0
31	200560.0
32	266031.0
33	364026.0
34	533208.0
35	837069.0
36	1505383.0
37	2653713.0
38	2798265.0
39	931452.0
40	13205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.34721439336312	20.089499590891187	12.889704888100436	24.67358112764526
2	14.91847096594771	21.386857038444436	40.8942677924476	22.80040420316025
3	18.495039738957633	26.001587987539477	30.196223670628182	25.307148602874708
4	12.420026018630914	17.506083567724332	38.91092120109631	31.162969212548447
5	12.908203070479857	38.10159012230727	35.97862615211598	13.011580655096894
6	30.397900312536404	38.0674048729335	18.584593996488852	12.95010081804125
7	26.562190548474547	32.37282260858227	23.56514084423746	17.499845998705727
8	24.39378608799147	37.217130848488935	21.42968461765051	16.95939844586909
9	25.917864273147483	15.902452788786018	21.139215939905146	37.04046699816136
10-11	24.399711564997396	26.606496714086415	29.21697920967655	19.77681251123964
12-13	24.826327826258847	24.17659542074157	29.34121766915596	21.65585908384362
14-15	21.89217212093857	25.502960917497646	26.695337876726573	25.909529084837214
16-17	21.760132126013655	28.600694562396452	27.401414805868953	22.237758505720947
18-19	21.81035404949758	28.05840742473615	28.779525001557772	21.351713524208503
20-21	22.575057712134885	27.38328489272292	28.536075058161614	21.505582336980577
22-23	22.231800345683087	27.355335110454305	28.423878933697132	21.988985610165475
24-25	21.99750884750051	27.391816148989072	28.57848083994783	22.032194163562586
26-27	22.0332579228791	27.586137681298013	28.427309516960914	21.953294878861975
28-29	21.966946498851357	27.70443786945811	28.391800364037945	21.936815267652587
30-31	22.197287776716497	27.563933921316398	28.31953678465456	21.919241517312546
32-33	21.965460123719964	27.74102935200302	28.323335448407633	21.970175075869378
34-35	22.06148639707352	27.73036955950012	28.33935987444882	21.868784168977538
36-37	22.102652067061765	27.673119217592028	28.28036636275135	21.943862352594852
38-39	21.964810970457034	27.788596561875305	28.36539661284254	21.88119585482512
40-41	22.184264997333422	27.687233348305117	28.221903159466166	21.90659849489529
42-43	22.056462255850782	27.777228973541646	28.099719860039023	22.066588910568548
44-45	22.15611284160343	27.88368575914814	27.95299148650053	22.007209912747904
46-47	22.181371725509432	27.803795209226266	27.78859156438341	22.226241500880892
48-49	22.16880166543701	27.868665885721207	27.815546386471972	22.146986062369812
50-51	22.07054197231243	28.008929648181436	27.748239848266444	22.172288531239694
52-53	22.279783057998458	27.902270379448385	27.596243730969032	22.22170283158412
54-55	22.25493322108441	27.824485402229094	27.666228262160537	22.25435311452596
56-57	22.233623602857357	27.915665556805063	27.680120950904197	22.17058988943338
58-59	22.231516654698723	27.97316982664167	27.634426513595457	22.16088700506415
60-61	22.302183151223957	27.925970663386266	27.639043526213953	22.13280265917582
62-63	22.24974638301875	27.99258590225501	27.606050948460325	22.15161676626592
64-65	22.23563015294067	27.957792043294248	27.62374964156101	22.18282816220408
66-67	22.272292780552664	27.98552901154896	27.473878490398256	22.268299717500117
68-69	22.194019083500464	27.988504835406353	27.566782437500827	22.250693643592356
70-71	22.22092717939836	27.79011776334456	27.62505406487264	22.36390099238444
72-73	22.25951401523679	27.823353661901628	27.620531518186652	22.29660080467493
74-75	22.096388281025543	27.96035463160573	27.594342410174267	22.348914677194465
76-77	22.203896055194107	27.921184678227274	27.524603947229775	22.350315319348844
78-79	22.18085669541	27.94212941995321	27.490707094117568	22.386306790519225
80-81	22.18904499674455	27.947118288809264	27.50021379322328	22.363622921222902
82-83	22.224110162500796	27.89071701677187	27.641581562117736	22.243591258609598
84-85	22.358997551531765	27.88786257662797	27.520890716139156	22.23224915570111
86-87	22.1475070517815	27.916369678448593	27.60285312355133	22.333270146218574
88-89	22.236418647166577	27.863907796961957	27.571012338974967	22.328661216896506
90-91	22.26270379550747	27.934720013113655	27.596975039716025	22.205601151662847
92-93	22.258613766908606	27.934875063016307	27.55562056286412	22.250890607210966
94-95	22.124784102902012	27.97463374261204	27.650733426442404	22.249848728043546
96-97	22.12115732210154	27.98094288345327	27.691747797756943	22.20615199668825
98-99	22.38465517719687	28.582898303342237	27.71559727346755	21.316849245993343
100-101	23.124409911685532	31.180771573377186	24.837783293264614	20.85703522167267
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1681.0
1	1504.0
2	2226.0
3	3391.5
4	4860.5
5	8152.0
6	10800.5
7	12458.0
8	13264.0
9	12610.0
10	11136.5
11	9611.5
12	10134.5
13	13465.5
14	21277.5
15	31507.5
16	39069.0
17	42919.5
18	44061.5
19	43181.5
20	41554.0
21	40910.0
22	43114.5
23	50000.0
24	61426.5
25	76844.0
26	95960.5
27	115479.0
28	134026.5
29	153891.0
30	172431.0
31	192065.0
32	216318.5
33	240076.0
34	263597.5
35	287303.0
36	311807.5
37	332790.5
38	347803.5
39	361479.0
40	377098.0
41	393050.5
42	403262.5
43	411828.0
44	422889.0
45	432045.5
46	437969.0
47	441576.0
48	441224.0
49	434786.5
50	426181.5
51	415052.5
52	397244.0
53	378648.0
54	359162.0
55	334439.0
56	307323.0
57	277548.0
58	245108.0
59	212975.5
60	179499.0
61	146947.0
62	118466.5
63	92669.0
64	69388.0
65	51340.5
66	38530.0
67	29308.5
68	22128.5
69	15983.0
70	11336.0
71	7993.0
72	5494.5
73	3695.5
74	2487.0
75	1661.0
76	1138.5
77	766.5
78	520.5
79	352.0
80	241.0
81	166.5
82	123.0
83	85.5
84	57.5
85	44.5
86	35.0
87	28.0
88	21.0
89	13.5
90	8.0
91	7.5
92	7.0
93	5.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013247423163289724
3	0.0
4	0.0026589470777745807
5	3.595729144321497E-4
6	4.1634758513196276E-4
7	0.0
8	0.0
9	0.0
10-11	2.602172407074767E-4
12-13	4.731222558317759E-4
14-15	4.731222558317759E-6
16-17	0.0
18-19	0.0011496870816712153
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.2074656689541175E-5
28-29	0.0
30-31	5.6913487657100785E-5
32-33	8.460225627563377E-4
34-35	6.861923003410328E-4
36-37	3.96960753685948E-4
38-39	0.0015289936870350903
40-41	4.836889680655484E-6
42-43	3.406369960488542E-5
44-45	1.4702103768134494E-5
46-47	1.9764240283355962E-5
48-49	0.0
50-51	5.018573237695387E-6
52-53	5.053530277493895E-6
54-55	0.0029564205796792746
56-57	0.0026761055609832423
58-59	0.0014518393124915686
60-61	1.5102775952714355E-4
62-63	2.0994778808458122E-5
64-65	1.2692770124096155E-4
66-67	3.198714372719317E-5
68-69	1.3443989918083082E-4
70-71	0.0
72-73	0.0
74-75	9.941321899782196E-5
76-77	0.0
78-79	4.048261347656082E-4
80-81	0.0
82-83	1.5488479984093905E-4
84-85	2.3174725320119723E-5
86-87	0.0014455379812471
88-89	0.0
90-91	7.78862440251515E-5
92-93	3.0358322928524124E-5
94-95	1.1087823260097234E-4
96-97	3.1351769804270274E-5
98-99	6.527489280883478E-6
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	201.0
20-21	725.0
22-23	1473.0
24-25	2930.0
26-27	5154.0
28-29	10152.0
30-31	21734.0
32-33	25518.0
34-35	33977.0
36-37	51546.0
38-39	62490.0
40-41	61192.0
42-43	68443.0
44-45	82213.0
46-47	82099.0
48-49	77248.0
50-51	70460.0
52-53	66854.0
54-55	72056.0
56-57	75205.0
58-59	76257.0
60-61	75817.0
62-63	72206.0
64-65	73688.0
66-67	80170.0
68-69	82820.0
70-71	82851.0
72-73	79642.0
74-75	80815.0
76-77	78352.0
78-79	86280.0
80-81	89475.0
82-83	86566.0
84-85	85732.0
86-87	92136.0
88-89	101192.0
90-91	109326.0
92-93	115208.0
94-95	131715.0
96-97	216677.0
98-99	945925.0
100-101	6853573.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.99184273178224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.28452592561675	73.26895491268736
2	7.877086606756819	13.074678657051805
3	2.1311168668028704	5.30595947558257
4	0.7970828657925847	2.646055033682259
5	0.36810878095430616	1.5275013028573927
6	0.1859562412121902	0.925971067540527
7	0.11548518145403032	0.6709029611958954
8	0.06658943431450218	0.4421103888182007
9	0.04152440914324746	0.3101568509831916
>10	0.12987190417472436	1.5885238149818688
>50	0.002133546161618534	0.11683019318800839
>100	4.4072777102082013E-4	0.05965388212912483
>500	3.875662018699828E-5	0.018414363744485063
>1k	3.875322513259646E-5	0.04428709555725535
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8924890233271036E-5
2	0.0	2.8387335349906553E-5	0.0	0.0	1.8924890233271036E-5
3	0.0	3.784978046654207E-5	0.0	9.462445116635518E-6	1.8924890233271036E-5
4	0.0	4.731222558317759E-5	9.462445116635518E-6	2.8387335349906553E-5	1.8924890233271036E-5
5	0.0	5.677467069981311E-5	9.462445116635518E-6	2.8387335349906553E-5	2.8387335349906553E-5
6	9.462445116635518E-5	5.677467069981311E-5	9.462445116635518E-6	2.8387335349906553E-5	3.784978046654207E-5
7	9.462445116635518E-5	5.677467069981311E-5	9.462445116635518E-6	2.8387335349906553E-5	4.731222558317759E-5
8	1.040868962829907E-4	5.677467069981311E-5	9.462445116635518E-6	2.8387335349906553E-5	5.677467069981311E-5
9	1.040868962829907E-4	1.3247423163289726E-4	9.462445116635518E-6	6.623711581644863E-5	7.569956093308414E-5
10-11	1.1354934139962621E-4	1.3247423163289726E-4	9.462445116635518E-6	6.623711581644863E-5	7.569956093308414E-5
12-13	1.2301178651626172E-4	1.3720545419121502E-4	9.462445116635518E-6	6.623711581644863E-5	8.04307834914019E-5
14-15	1.3247423163289726E-4	2.3182990535757018E-4	9.462445116635518E-6	1.040868962829907E-4	1.0881811884130846E-4
16-17	1.608615669828038E-4	2.412923504742057E-4	9.462445116635518E-6	1.040868962829907E-4	1.2301178651626172E-4
18-19	1.608615669828038E-4	2.7441090838243004E-4	9.462445116635518E-6	1.040868962829907E-4	1.277430090745795E-4
20-21	1.608615669828038E-4	3.122606888489721E-4	9.462445116635518E-6	1.1354934139962621E-4	1.5139912186616828E-4
22-23	1.6559278954112155E-4	3.217231339656076E-4	9.462445116635518E-6	1.3720545419121502E-4	1.6559278954112155E-4
24-25	1.7032401209943933E-4	3.501104693155142E-4	9.462445116635518E-6	1.7978645721607482E-4	1.8924890233271036E-4
26-27	1.7032401209943933E-4	3.595729144321497E-4	9.462445116635518E-6	2.3656112791588794E-4	1.8924890233271036E-4
28-29	1.7032401209943933E-4	3.595729144321497E-4	9.462445116635518E-6	5.015095911816824E-4	1.9871134744934587E-4
30-31	1.7032401209943933E-4	4.305412528069161E-4	9.462445116635518E-6	0.001277430090745795	1.9871134744934587E-4
32-33	1.750552346577571E-4	4.3527247536523383E-4	9.462445116635518E-6	0.002748840306382618	1.9871134744934587E-4
34-35	1.7978645721607485E-4	5.535530393231778E-4	9.462445116635518E-6	0.004295950082952525	1.9871134744934587E-4
36-37	1.7978645721607485E-4	6.623711581644862E-4	9.462445116635518E-6	0.0067467233681611245	1.9871134744934587E-4
38-39	1.7978645721607485E-4	6.718336032811217E-4	9.462445116635518E-6	0.010635788311098323	2.0344257000766365E-4
40-41	1.7978645721607485E-4	7.475331642142059E-4	9.462445116635518E-6	0.015225074192666548	2.081737925659814E-4
42-43	1.845176797743926E-4	7.806517221224303E-4	9.462445116635518E-6	0.01898166490397085	2.081737925659814E-4
44-45	1.939801248910281E-4	9.083947311970097E-4	9.462445116635518E-6	0.023078903639474027	2.1763623768261692E-4
46-47	1.9871134744934587E-4	0.0010408689628299069	9.462445116635518E-6	0.027961525319657954	2.2709868279925243E-4
48-49	1.9871134744934587E-4	0.0010408689628299069	9.462445116635518E-6	0.03295296511868319	2.4602357303252345E-4
50-51	2.55486018149159E-4	0.0011544183042295332	9.462445116635518E-6	0.037722037457467494	2.5548601814915896E-4
52-53	2.649484632657945E-4	0.0011544183042295332	9.462445116635518E-6	0.042046374875769926	2.649484632657945E-4
54-55	2.649484632657945E-4	0.0011686119719044865	9.462445116635518E-6	0.04651738019338021	2.791421309407478E-4
56-57	2.649484632657945E-4	0.0012017305298127109	9.462445116635518E-6	0.05100731040122376	3.169919114072899E-4
58-59	2.649484632657945E-4	0.0012064617523710286	9.462445116635518E-6	0.055554015279767124	3.501104693155142E-4
60-61	2.9333579861570106E-4	0.0012253866426042996	9.462445116635518E-6	0.060209538277151795	3.5484169187383193E-4
62-63	3.217231339656076E-4	0.0012726988681874773	9.462445116635518E-6	0.0647420494880202	3.784978046654207E-4
64-65	3.217231339656076E-4	0.0014193667674953277	9.462445116635518E-6	0.06903326834841442	3.784978046654207E-4
66-67	3.217231339656076E-4	0.0014477541028452342	9.462445116635518E-6	0.07340018676974172	3.8322902722373847E-4
68-69	3.217231339656076E-4	0.0014950663284284118	9.462445116635518E-6	0.07789484820014358	3.9742269489869173E-4
70-71	3.406480241988786E-4	0.0015281848863366362	9.462445116635518E-6	0.08243209063357032	4.0688514001532725E-4
72-73	4.163475851319628E-4	0.0015376473314532716	9.462445116635518E-6	0.08607986322603331	4.258100302485983E-4
74-75	4.163475851319628E-4	0.0015565722216865428	9.462445116635518E-6	0.08918354522428976	4.305412528069161E-4
76-77	4.2107880769028057E-4	0.0015991532247114026	9.462445116635518E-6	0.09169582440275649	4.3527247536523383E-4
78-79	4.258100302485983E-4	0.0016180781149446736	9.462445116635518E-6	0.09436896514820602	4.400036979235516E-4
80-81	4.400036979235516E-4	0.0016228093375029913	9.462445116635518E-6	0.0956132766810436	4.683910332734581E-4
82-83	4.494661430401871E-4	0.001627540560061309	9.462445116635518E-6	0.09616682972036678	4.920471460650469E-4
84-85	4.6365981071514037E-4	0.00164646545029458	9.462445116635518E-6	0.09667307053410676	5.015095911816824E-4
86-87	4.731222558317759E-4	0.0016559278954112156	9.462445116635518E-6	0.09681027598829797	5.109720362983179E-4
88-89	4.920471460650469E-4	0.0017410899014609353	9.462445116635518E-6	0.0968670506589978	5.204344814149534E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10490	0.0	44.288357	1
GTATCAA	17140	0.0	33.88793	1
TCAACGC	19805	0.0	28.301764	4
ATCAACG	20145	0.0	27.75974	3
CAACGCA	20250	0.0	27.614754	5
AACGCAG	20995	0.0	26.737644	6
TATCAAC	21865	0.0	26.070654	2
GTACATG	29245	0.0	23.289555	1
TACATGG	28565	0.0	23.269053	2
ACGCAGA	24505	0.0	22.85516	7
ACATGGG	28700	0.0	22.451437	3
CGCAGAG	25250	0.0	22.18082	8
CATGGGG	19855	0.0	20.74299	4
GCAGAGT	31715	0.0	17.659332	9
AGAGTAC	27495	0.0	17.375568	10-11
GAGTACA	19830	0.0	17.129963	1
GAGTACT	17540	0.0	16.938826	12-13
CAGAGTA	30550	0.0	16.599916	10-11
ATGGGGG	12805	0.0	15.794222	5
AGTACAT	20700	0.0	15.51226	2
>>END_MODULE