FastQCFastQC Report
Fri 27 May 2016
SRR2138820_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138820_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10619856
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96250.045.7670971
GTATCAA164500.033.287321
TCAACGC185350.028.5048734
ATCAACG187300.028.1147793
CAACGCA192100.027.5252445
AACGCAG199050.026.6300376
TATCAAC210750.025.443132
GTACATG247500.024.031261
TACATGG244750.023.2835122
ACGCAGA232950.022.717517
ACATGGG249200.022.463973
CGCAGAG236550.022.3163558
CATGGGG177550.019.5673454
AGAGTAC262400.017.78634510-11
GCAGAGT301150.017.4712079
GAGTACT179850.017.1909312-13
CAGAGTA289200.016.52341510-11
ATGGGGG117600.015.6811635
GAGTACA174100.015.5376541
GTGGTAT61500.014.8513111
Produced by FastQC (version 0.11.5)