##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138820_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10619856
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43291001309245	34.0	31.0	34.0	31.0	34.0
2	32.64208488326019	34.0	31.0	34.0	31.0	34.0
3	32.73989609651957	34.0	31.0	34.0	31.0	34.0
4	36.20855885428202	37.0	37.0	37.0	35.0	37.0
5	36.05783967315564	37.0	35.0	37.0	35.0	37.0
6	36.09442811653943	37.0	35.0	37.0	35.0	37.0
7	36.07879852608171	37.0	35.0	37.0	35.0	37.0
8	36.03639587956748	37.0	35.0	37.0	35.0	37.0
9	37.880438680147826	39.0	38.0	39.0	35.0	39.0
10-11	37.758447525088854	39.0	37.5	39.0	35.0	39.0
12-13	37.78029678556847	39.0	37.5	39.0	35.0	39.0
14-15	39.13768364655792	40.0	38.5	41.0	36.0	41.0
16-17	39.10832430307906	40.0	38.0	41.0	36.0	41.0
18-19	39.133873331239144	40.0	38.5	41.0	36.0	41.0
20-21	39.129032166665056	40.0	39.0	41.0	36.0	41.0
22-23	39.093150346748175	40.0	39.0	41.0	36.0	41.0
24-25	39.099087840792535	40.0	39.0	41.0	36.0	41.0
26-27	38.97800744924977	40.0	38.5	41.0	36.0	41.0
28-29	38.89128766645603	40.0	38.0	41.0	35.0	41.0
30-31	38.794407902198486	40.0	38.0	41.0	35.0	41.0
32-33	38.714332669095015	40.0	38.0	41.0	35.0	41.0
34-35	38.65050778257033	40.0	38.0	41.0	35.0	41.0
36-37	38.618891363693294	40.0	38.0	41.0	35.0	41.0
38-39	38.55230913336355	40.0	38.0	41.0	35.0	41.0
40-41	38.522479605654894	40.0	38.0	41.0	34.5	41.0
42-43	38.49322709343765	40.0	38.0	41.0	34.0	41.0
44-45	38.426713428482884	40.0	38.0	41.0	34.0	41.0
46-47	38.34599566861566	40.0	38.0	41.0	34.0	41.0
48-49	38.30926258075873	40.0	38.0	41.0	34.0	41.0
50-51	38.23962790091622	40.0	38.0	41.0	34.0	41.0
52-53	38.06874801684886	40.0	37.0	41.0	33.0	41.0
54-55	37.92889309585149	40.0	37.0	41.0	33.0	41.0
56-57	37.78511902157666	39.5	37.0	41.0	33.0	41.0
58-59	37.62645643967218	39.0	36.0	41.0	33.0	41.0
60-61	37.49548894540392	39.0	36.0	41.0	33.0	41.0
62-63	37.296756940187244	39.0	35.5	40.0	32.0	41.0
64-65	37.073233365078124	39.0	35.0	40.0	32.0	41.0
66-67	36.842089498818794	38.0	35.0	40.0	32.0	41.0
68-69	36.56963605045595	37.5	35.0	40.0	31.5	41.0
70-71	36.266113251309626	37.0	35.0	39.5	31.0	41.0
72-73	35.95169948988563	36.5	35.0	39.0	31.0	41.0
74-75	35.55698225819761	36.0	34.0	39.0	31.0	40.0
76-77	33.56385413093829	34.5	32.0	36.5	28.0	39.0
78-79	34.71019702567477	35.0	34.0	37.0	30.0	39.0
80-81	34.694811611169456	35.0	34.0	37.0	30.5	39.0
82-83	34.425351497121355	35.0	34.0	36.5	30.0	39.0
84-85	34.11429614043192	35.0	34.0	36.0	30.0	37.0
86-87	33.92647681743189	35.0	34.0	36.0	30.0	37.0
88-89	33.73597706588975	35.0	34.0	35.0	30.0	37.0
90-91	33.56369886646597	35.0	33.0	35.0	30.0	36.0
92-93	33.482730386079155	35.0	33.0	35.0	30.0	36.0
94-95	33.41531432891583	35.0	33.0	35.0	30.0	36.0
96-97	33.44538178982169	35.0	33.0	35.0	30.0	36.0
98-99	33.49410280030678	35.0	33.0	35.0	30.0	35.0
100-101	32.22952535686299	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	9.0
20	41.0
21	180.0
22	778.0
23	2787.0
24	7354.0
25	16471.0
26	31115.0
27	51466.0
28	77412.0
29	108806.0
30	146473.0
31	192200.0
32	253706.0
33	346256.0
34	506283.0
35	791465.0
36	1429871.0
37	2647161.0
38	3004917.0
39	990934.0
40	14169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.961341095397145	20.227637738214153	13.305820719226325	24.505200447162373
2	14.646640134873715	21.183163912543016	41.5174159148572	22.65278003772607
3	18.33477779736373	25.85864629426237	30.33759591467153	25.468979993702362
4	12.311829379603715	17.58584708524871	39.162051201599766	30.940272333547814
5	12.863592658894216	38.04186079665968	36.21326996500975	12.88127657943635
6	30.372165827087848	38.24968038502388	18.564326345228995	12.813827442659278
7	26.502590995584118	32.38070271385977	23.593615581981524	17.52309070857458
8	24.03906418316783	37.70303476807972	21.334724312646046	16.923176736106402
9	25.493857920484043	16.25749916006394	21.359639904721874	36.88900301473014
10-11	24.058063308405732	26.79842938987113	29.427922614898815	19.715584686824318
12-13	24.608830891769664	24.32507788871155	29.519912310962184	21.546178908556605
14-15	21.632099573492273	25.787170624606958	26.857011082402305	25.72371871949846
16-17	21.598075341134567	28.879402884558885	27.60923500280983	21.91328677149671
18-19	21.62865683008952	28.348927390105473	28.930014460791032	21.092401319013977
20-21	22.23810647076355	27.60694324186511	28.851531634859718	21.30341865251162
22-23	21.942187727413582	27.58194328578701	28.70814183407877	21.767727152720642
24-25	21.74019333932659	27.64693407825681	28.80955835677349	21.80331422564311
26-27	21.797197732773505	27.779366150992622	28.68388149168643	21.739554624547438
28-29	21.74503392029526	27.895211800044073	28.67229235676821	21.68746192289246
30-31	21.94727361873558	27.758944755086024	28.633999200600634	21.65978242557776
32-33	21.751948540101985	27.88115619289424	28.601528572678127	21.765366694325653
34-35	21.865987639975327	27.92206610539421	28.61421819867862	21.59772805595184
36-37	21.852247820324042	27.869438571346457	28.548524060932557	21.729789547396944
38-39	21.735278528678283	27.975338172724694	28.62554540597923	21.66383789261779
40-41	21.93511280966743	27.872743235251697	28.513786482430753	21.67835747265012
42-43	21.821417797276744	27.966923653894643	28.369564173162072	21.84209437566654
44-45	21.924061482772835	28.01443184321137	28.250509430327043	21.810997243688753
46-47	21.94654077371163	28.034380137077804	28.070908836989812	21.948170252220763
48-49	21.935741138475997	28.075819685056754	28.074553218028658	21.91388595843859
50-51	21.88849242571474	28.208943467142383	28.02149730539632	21.881066801746556
52-53	22.049173546337066	28.10197444486172	27.91087627810871	21.937975730692504
54-55	21.988082067445596	28.03548884762647	27.996228251996904	21.98020083293103
56-57	21.984389135298958	28.131054060519954	27.98593976298992	21.89861704119117
58-59	21.98925655481385	28.16960625339516	27.9237409691379	21.917396222653096
60-61	22.01444537305606	28.144762015961195	27.958561730822808	21.882230880159938
62-63	21.9956495924193	28.190863834758545	27.921336118399797	21.892150454422357
64-65	21.964628443394332	28.175392141183174	27.918686871255264	21.941292544167222
66-67	22.018779791666244	28.19586155247929	27.817834860797564	21.967523795056902
68-69	21.976212287770082	28.19246509769991	27.875914678154263	21.955407936375746
70-71	21.955171207510066	28.048982730757636	27.916672104453035	22.079173957279266
72-73	21.99223645000408	28.066576054853755	27.87829799326516	22.062889501877006
74-75	21.89710913984025	28.158901713339684	27.879827637370656	22.06416150944941
76-77	22.028552001011537	28.11574918477509	27.78342937674977	22.072269437463607
78-79	21.96319252578781	28.103559089028163	27.824510926096146	22.108737459087873
80-81	21.950689474020578	28.175059695633465	27.816105631839054	22.0581451985069
82-83	22.00113761365012	28.133944273173235	27.87076682235339	21.994151290823265
84-85	22.099417603876947	28.115719399435168	27.801489277539442	21.983373719148442
86-87	21.95239153523414	28.11793350493402	27.89980222514958	22.029872734682268
88-89	21.993026793438595	28.09099142642191	27.877959000342884	22.038022779796616
90-91	21.98741485969071	28.1572563946034	27.87350348345098	21.981825262254905
92-93	22.01267237121197	28.12118708500414	27.89752917266959	21.968611371114303
94-95	21.881971498929875	28.17545989782323	27.937789279306962	22.004779323939935
96-97	21.918190380807662	28.20643000685092	27.967095625131005	21.908283987210414
98-99	22.172980263997143	28.74511660730345	28.025452492184165	21.056450636515237
100-101	22.87589696117271	31.418361795437434	25.1927569013007	20.512984342089155
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2010.0
1	1708.0
2	2203.0
3	3258.0
4	4472.5
5	7292.5
6	9703.0
7	11443.5
8	12651.5
9	12495.5
10	11148.0
11	9519.5
12	9910.0
13	13022.5
14	20404.5
15	30128.0
16	37586.0
17	41736.0
18	43351.0
19	43259.0
20	42474.5
21	42408.5
22	45166.0
23	52329.5
24	64329.0
25	80604.0
26	99464.0
27	118806.0
28	139076.0
29	160056.5
30	180746.0
31	203887.5
32	228305.0
33	251346.0
34	273804.0
35	295235.0
36	317885.5
37	338175.0
38	356609.0
39	373436.0
40	388001.0
41	402134.5
42	411768.0
43	419855.5
44	427038.0
45	433989.0
46	438124.0
47	438575.5
48	436687.0
49	428837.5
50	418243.5
51	402597.0
52	380396.0
53	358546.5
54	336386.0
55	310477.5
56	282701.5
57	253942.5
58	223588.5
59	192986.0
60	161367.0
61	131049.0
62	104457.5
63	80674.0
64	60551.0
65	45422.0
66	34442.0
67	26271.0
68	19845.5
69	14470.0
70	10481.0
71	7715.0
72	5699.5
73	4032.0
74	2808.0
75	1925.5
76	1272.5
77	843.0
78	578.0
79	407.0
80	280.5
81	200.0
82	142.5
83	89.0
84	57.0
85	42.5
86	33.0
87	27.5
88	24.0
89	16.5
90	9.5
91	4.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001355950589160531
3	0.0
4	0.0028719786784302914
5	3.672366178976438E-4
6	4.4256720618434E-4
7	0.0
8	0.0
9	0.0
10-11	3.2015500021845874E-4
12-13	5.320222797747916E-4
14-15	1.4124485303755531E-5
16-17	0.0
18-19	9.887139712628872E-4
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.7109415716520784E-5
28-29	0.0
30-31	5.190902273667105E-5
32-33	9.834209413248514E-4
34-35	7.629703658992622E-4
36-37	3.6608504469375417E-4
38-39	0.0016622712012660773
40-41	9.603355489234782E-6
42-43	2.413795633771975E-5
44-45	3.4005731909011354E-5
46-47	3.42532902609626E-5
48-49	9.85577356636193E-6
50-51	2.4801677109247467E-5
52-53	1.4970086025599347E-5
54-55	0.0031072182034194862
56-57	0.0028596616979792043
58-59	0.0013987340693726322
60-61	1.4850935780508085E-4
62-63	2.0621461776032676E-5
64-65	9.859498513732492E-5
66-67	4.702862193283247E-5
68-69	1.3687054552177473E-4
70-71	0.0
72-73	0.0
74-75	1.2925564796017894E-4
76-77	1.6280075472259213E-5
78-79	3.77327165291938E-4
80-81	0.0
82-83	1.3905878009072084E-4
84-85	2.2440195056907494E-5
86-87	0.0014883684290231617
88-89	0.0
90-91	6.929248218143821E-5
92-93	2.336523388715947E-5
94-95	2.1888720937324725E-4
96-97	1.8033480238070795E-5
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	185.0
20-21	681.0
22-23	1366.0
24-25	3058.0
26-27	4899.0
28-29	9633.0
30-31	19433.0
32-33	22930.0
34-35	30567.0
36-37	45706.0
38-39	54900.0
40-41	54860.0
42-43	61602.0
44-45	73359.0
46-47	73161.0
48-49	67808.0
50-51	61352.0
52-53	58068.0
54-55	63649.0
56-57	65818.0
58-59	66303.0
60-61	65970.0
62-63	63091.0
64-65	65591.0
66-67	69758.0
68-69	73164.0
70-71	72142.0
72-73	69373.0
74-75	71088.0
76-77	68517.0
78-79	74845.0
80-81	78632.0
82-83	76918.0
84-85	76363.0
86-87	82302.0
88-89	90092.0
90-91	97602.0
92-93	104592.0
94-95	122562.0
96-97	210656.0
98-99	967899.0
100-101	7179361.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.89675833133917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.3737419505922	75.87540971546896
2	7.416129258221877	12.592106667784728
3	1.8411366319260873	4.689195950868143
4	0.6624754038441072	2.24968057042438
5	0.2921244836844542	1.240021084701317
6	0.14879788983656245	0.7579475096200723
7	0.07931273082074741	0.4713375618761284
8	0.04924085948723707	0.3344311478332316
9	0.0340087856002342	0.25985120870208694
>10	0.1005918952376755	1.3366333168894102
>50	0.0019987153412968574	0.11026212355672185
>100	3.8767346294560644E-4	0.052490224995943344
>500	5.372194455043621E-5	0.030632917278882364
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.8832647071674043E-5	0.0	0.0	0.0
5	0.0	1.8832647071674043E-5	0.0	0.0	0.0
6	0.0	1.8832647071674043E-5	0.0	0.0	1.8832647071674043E-5
7	0.0	1.8832647071674043E-5	0.0	9.416323535837021E-6	1.8832647071674043E-5
8	0.0	1.8832647071674043E-5	0.0	9.416323535837021E-6	1.8832647071674043E-5
9	0.0	7.533058828669617E-5	0.0	1.8832647071674043E-5	1.8832647071674043E-5
10-11	0.0	8.945507359045169E-5	0.0	2.8248970607511062E-5	2.3540808839592554E-5
12-13	0.0	9.41632353583702E-5	0.0	5.178977944710361E-5	2.8248970607511062E-5
14-15	0.0	1.2241220596588128E-4	0.0	5.6497941215022125E-5	3.295713237542958E-5
16-17	0.0	1.2241220596588128E-4	0.0	7.062242651877766E-5	3.7665294143348085E-5
18-19	0.0	1.2712036773379978E-4	0.0	1.0828772066212575E-4	4.70816176791851E-5
20-21	0.0	1.4595301480547384E-4	0.0	1.1299588243004425E-4	4.70816176791851E-5
22-23	0.0	2.1186727955633297E-4	0.0	1.459530148054738E-4	6.120610298294064E-5
24-25	0.0	2.3069992662800703E-4	0.0	1.789101471809034E-4	7.533058828669617E-5
26-27	0.0	2.4482441193176256E-4	0.0	2.448244119317625E-4	8.474691182253319E-5
28-29	0.0	2.4482441193176256E-4	0.0	5.508549268464657E-4	8.474691182253319E-5
30-31	0.0	2.919060296109476E-4	0.0	0.001577234192252701	8.474691182253319E-5
32-33	0.0	3.013223531467847E-4	0.0	0.0032156744874883426	1.2241220596588128E-4
34-35	9.416323535837021E-6	3.436958090580513E-4	0.0	0.005004775959297376	1.3182852950171828E-4
36-37	9.416323535837021E-6	3.907774267372364E-4	0.0	0.007914419931871017	1.3182852950171828E-4
38-39	9.416323535837021E-6	4.2373455911266593E-4	0.0	0.012344800155482335	1.3182852950171828E-4
40-41	9.416323535837021E-6	4.6610801502393253E-4	0.0	0.016826970158540757	1.3653669126963678E-4
42-43	9.416323535837021E-6	4.896488238635251E-4	0.0	0.02095602802900529	1.412448530375553E-4
44-45	9.416323535837021E-6	5.461467650785471E-4	0.0	0.02553236126742208	1.4595301480547384E-4
46-47	1.4124485303755531E-5	5.838120592218953E-4	0.0	0.030838459579866244	1.5536933834131084E-4
48-49	1.8832647071674043E-5	5.885202209898138E-4	0.0	0.0360927681128633	1.6949382364506637E-4
50-51	1.8832647071674043E-5	6.214773533652434E-4	0.0	0.04118699914575113	1.6949382364506637E-4
52-53	1.8832647071674043E-5	6.591426475085915E-4	0.0	0.046163526134440996	1.8832647071674043E-4
54-55	1.8832647071674043E-5	6.685589710444284E-4	0.0	0.050918769520038695	2.0715911778841447E-4
56-57	1.8832647071674043E-5	6.685589710444284E-4	0.0	0.05570697003801181	2.1186727955633297E-4
58-59	1.8832647071674043E-5	6.82683456348184E-4	0.0	0.05999139724681766	2.1657544132425147E-4
60-61	1.8832647071674043E-5	7.062242651877766E-4	0.0	0.06437940401451772	2.1657544132425147E-4
62-63	1.8832647071674043E-5	7.156405887236136E-4	0.0	0.06838604967901636	2.3540808839592553E-4
64-65	1.8832647071674043E-5	8.380527946894948E-4	0.0	0.07234090556406791	2.3540808839592553E-4
66-67	1.8832647071674043E-5	8.380527946894948E-4	0.0	0.07632401041972697	2.3540808839592553E-4
68-69	1.8832647071674043E-5	8.427609564574134E-4	0.0	0.08068847637858742	2.3540808839592553E-4
70-71	1.8832647071674043E-5	8.568854417611689E-4	0.0	0.08483165873435572	2.3540808839592553E-4
72-73	1.8832647071674043E-5	8.568854417611689E-4	0.0	0.08822624336902496	2.730733825392736E-4
74-75	1.8832647071674043E-5	8.710099270649245E-4	0.0	0.09080631601784431	2.824897060751106E-4
76-77	1.8832647071674043E-5	8.94550735904517E-4	0.0	0.09294852962224723	2.824897060751106E-4
78-79	1.8832647071674043E-5	9.22799706512028E-4	0.0	0.09495891469714843	2.824897060751106E-4
80-81	1.8832647071674043E-5	9.22799706512028E-4	0.0	0.09623482653625434	2.9661419137886615E-4
82-83	1.8832647071674043E-5	9.22799706512028E-4	0.0	0.09655968969824073	3.107386766826217E-4
84-85	1.8832647071674043E-5	9.22799706512028E-4	0.0	0.09686572021315543	3.107386766826217E-4
86-87	1.8832647071674043E-5	9.22799706512028E-4	0.0	0.09705404668387217	3.201550002184587E-4
88-89	1.8832647071674043E-5	0.0010263792654062352	0.0	0.09712937727215887	3.2957132375429574E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9625	0.0	45.767097	1
GTATCAA	16450	0.0	33.28732	1
TCAACGC	18535	0.0	28.504873	4
ATCAACG	18730	0.0	28.114779	3
CAACGCA	19210	0.0	27.525244	5
AACGCAG	19905	0.0	26.630037	6
TATCAAC	21075	0.0	25.44313	2
GTACATG	24750	0.0	24.03126	1
TACATGG	24475	0.0	23.283512	2
ACGCAGA	23295	0.0	22.71751	7
ACATGGG	24920	0.0	22.46397	3
CGCAGAG	23655	0.0	22.316355	8
CATGGGG	17755	0.0	19.567345	4
AGAGTAC	26240	0.0	17.786345	10-11
GCAGAGT	30115	0.0	17.471207	9
GAGTACT	17985	0.0	17.19093	12-13
CAGAGTA	28920	0.0	16.523415	10-11
ATGGGGG	11760	0.0	15.681163	5
GAGTACA	17410	0.0	15.537654	1
GTGGTAT	6150	0.0	14.851311	1
>>END_MODULE