FastQCFastQC Report
Fri 27 May 2016
SRR2138819_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138819_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16773346
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158800.047.271411
GTATCAA272850.034.446151
TCAACGC310350.029.202414
ATCAACG311850.029.0758363
CAACGCA320000.028.321695
AACGCAG332000.027.3635856
TATCAAC341650.026.8970782
ACGCAGA390350.023.2622137
GTACATG406900.023.2481271
TACATGG400950.023.125612
CGCAGAG395300.022.9378368
ACATGGG406000.021.9789263
CATGGGG298650.019.64714
GCAGAGT492800.018.3111489
AGAGTAC426200.018.03242510-11
GAGTACT295000.017.82137512-13
CAGAGTA476400.017.18916310-11
ATGGGGG192200.015.6498565
GAGTACA285900.015.5678111
AGTACTT334050.015.19651412-13
Produced by FastQC (version 0.11.5)