##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138819_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16773346
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.960932839518126	33.0	31.0	34.0	30.0	34.0
2	32.202407319326745	33.0	31.0	34.0	30.0	34.0
3	32.295847590576145	34.0	31.0	34.0	30.0	34.0
4	35.91940397580781	37.0	35.0	37.0	35.0	37.0
5	35.611461839516096	37.0	35.0	37.0	33.0	37.0
6	35.623225741602184	37.0	35.0	37.0	33.0	37.0
7	35.605432988742976	37.0	35.0	37.0	33.0	37.0
8	35.54749565173222	37.0	35.0	37.0	33.0	37.0
9	37.33547552169973	39.0	37.0	39.0	34.0	39.0
10-11	37.14971821960866	39.0	37.0	39.0	33.5	39.0
12-13	37.173535262433624	39.0	37.0	39.0	33.5	39.0
14-15	38.37522391179434	40.0	38.0	41.0	34.0	41.0
16-17	38.30990537010326	40.0	38.0	41.0	34.0	41.0
18-19	38.332415875759075	40.0	38.0	41.0	34.0	41.0
20-21	38.32694616153681	40.0	38.0	41.0	34.0	41.0
22-23	38.28001750583252	40.0	38.0	41.0	34.0	41.0
24-25	38.26673545317318	40.0	38.0	41.0	34.0	41.0
26-27	38.10865096236326	40.0	38.0	41.0	34.0	41.0
28-29	37.99998316199412	40.0	37.5	41.0	33.5	41.0
30-31	37.87598407243955	40.0	37.0	41.0	33.0	41.0
32-33	37.771533224112446	39.0	37.0	40.5	33.0	41.0
34-35	37.68253195590105	39.0	37.0	40.0	33.0	41.0
36-37	37.63982647364203	39.0	37.0	40.0	33.0	41.0
38-39	37.55421914388005	39.0	37.0	40.0	33.0	41.0
40-41	37.51501069743136	39.0	37.0	40.0	33.0	41.0
42-43	37.471652866055166	39.0	37.0	40.0	32.0	41.0
44-45	37.38856877155119	39.0	36.0	40.0	32.0	41.0
46-47	37.29254450845126	39.0	36.0	40.0	32.0	41.0
48-49	37.245733699618896	39.0	36.0	40.0	31.5	41.0
50-51	37.152385221479726	39.0	36.0	40.0	31.0	41.0
52-53	36.95263758983694	39.0	35.5	40.0	31.0	41.0
54-55	36.78614008313357	39.0	35.0	40.0	31.0	41.0
56-57	36.63349123167799	38.0	35.0	40.0	31.0	41.0
58-59	36.45676082368552	38.0	35.0	40.0	30.0	41.0
60-61	36.3236209976032	38.0	35.0	40.0	30.0	41.0
62-63	36.11529642474568	38.0	34.0	40.0	30.0	41.0
64-65	35.89250670043542	37.5	34.0	40.0	29.0	41.0
66-67	35.67177034447759	37.0	34.0	39.5	29.0	41.0
68-69	35.40437661602245	36.5	34.0	39.0	29.0	40.5
70-71	35.113247246932445	36.0	34.0	39.0	29.0	40.0
72-73	34.81554281093619	36.0	33.5	39.0	29.0	40.0
74-75	34.42265746373388	35.0	33.0	38.0	28.5	40.0
76-77	32.41317666953183	33.5	30.5	35.5	26.0	38.5
78-79	33.65557091212594	35.0	32.0	37.0	28.0	39.0
80-81	33.71478846479779	35.0	33.0	36.0	29.0	39.0
82-83	33.46876649162495	35.0	32.5	36.0	28.5	37.5
84-85	33.180997595061015	35.0	32.0	35.5	28.0	37.0
86-87	33.006362211700086	34.0	32.0	35.0	28.0	37.0
88-89	32.83380654783113	34.0	32.0	35.0	27.0	36.0
90-91	32.66247041724496	34.0	32.0	35.0	27.0	36.0
92-93	32.59507513718931	34.0	32.0	35.0	27.0	36.0
94-95	32.55275530458181	34.0	32.0	35.0	27.0	35.0
96-97	32.64029161957287	34.0	32.0	35.0	28.5	35.0
98-99	32.77275187320562	34.0	32.0	35.0	29.0	35.0
100-101	31.55132148035414	33.0	29.5	34.5	26.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	44.0
20	188.0
21	766.0
22	3103.0
23	9428.0
24	23934.0
25	49966.0
26	90273.0
27	143605.0
28	212686.0
29	292859.0
30	390376.0
31	508968.0
32	664945.0
33	898969.0
34	1268003.0
35	1886270.0
36	2894649.0
37	3869303.0
38	2933740.0
39	627749.0
40	3518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.93097787406281	20.433883615111736	13.399371836722382	24.23576667410307
2	15.035940032497045	21.457874094285305	40.932759593299274	22.57342627991838
3	18.569914434484332	25.960419584738787	30.43328385403842	25.03638212673846
4	12.681247661063274	17.67248572164273	38.73823541283004	30.908031204463953
5	12.892656013267754	38.08351737085026	36.090844610729874	12.932982005152116
6	30.57101681131232	38.2834696284074	18.256622180658784	12.888891379621493
7	26.532118278607026	32.42864601970293	23.47903036162254	17.560205340067508
8	24.25421856795895	37.625587643634134	21.188151725958555	16.932042062448364
9	25.606751330354722	16.135856256706322	21.178135835271032	37.07925657766793
10-11	24.246626626658255	26.771122914852835	29.246723059642417	19.735527398846493
12-13	24.712360026908293	24.276705521049756	29.400951281498894	21.609983170543057
14-15	21.762027140024035	25.746941777357414	26.6979565883264	25.79307449429215
16-17	21.660499342230228	28.8723967179834	27.435700664613965	22.031403275172405
18-19	21.750519586041037	28.25287421902613	28.81194435757476	21.18466183735807
20-21	22.404912295240916	27.55198319641117	28.676659249314696	21.366445259033217
22-23	22.07649709858906	27.483109637068132	28.590534050659222	21.849859213683587
24-25	21.911843725089863	27.53032575105439	28.690395075917667	21.86743544793808
26-27	21.93684680797443	27.70701497687934	28.560051663503728	21.796086551642503
28-29	21.877549860514467	27.822561543437107	28.56076906307996	21.739119532968463
30-31	22.11456545504714	27.66885472409028	28.50034025369289	21.71623956716969
32-33	21.877192305378824	27.82989750710842	28.47937454847735	21.813535639035415
34-35	22.00361812297657	27.83038419059593	28.44928644041735	21.716711246010156
36-37	22.036154416579784	27.752455207530275	28.43818165922584	21.7732087166641
38-39	21.87128819308419	27.895687556784154	28.505709448686627	21.727314801445026
40-41	22.07321231157077	27.816585115040365	28.348049294417883	21.762153278970985
42-43	21.974978501578995	27.86345038721595	28.265649249870666	21.89592186133439
44-45	22.065353415715354	27.95398013199803	28.09606787605948	21.884598576227138
46-47	22.09295338474044	27.934978305845213	27.915369624418833	22.05669868499551
48-49	22.075399710089602	28.01141785226204	27.93749846274043	21.975683974907934
50-51	22.04368510319356	28.1297652488591	27.860372984303655	21.966176663643683
52-53	22.192802005031517	28.035532148160353	27.770885915935068	22.000779930873062
54-55	22.157572116548643	27.950996643851507	27.810336569031573	22.081094670568284
56-57	22.13919364705643	28.06287684281582	27.80383071370457	21.994098796423177
58-59	22.124581755357227	28.11933797976673	27.771325232233725	21.984755032642322
60-61	22.175018666688786	28.072270006079997	27.795099727712252	21.957611599518962
62-63	22.141757658371038	28.12189586002452	27.753824169963774	21.982522311640665
64-65	22.09800888860513	28.09597489157238	27.780135863855275	22.025880355967214
66-67	22.185053382431136	28.117166469348494	27.618678800915543	22.07910134730483
68-69	22.111728791118104	28.128918800225488	27.70306278388901	22.0562896247674
70-71	22.102385976564417	27.952546002969953	27.74839049266352	22.19667752780211
72-73	22.162419451187308	27.989160682803384	27.72768592586749	22.120733940141815
74-75	21.992291641943527	28.118700297101224	27.717548402179972	22.17145965877528
76-77	22.096729650877435	28.07513783945629	27.65513211777299	22.173000391893286
78-79	22.082866987073977	28.099053195558742	27.64261375584652	22.17546606152076
80-81	22.07277878942936	28.1218619471894	27.648980820010998	22.15637844337024
82-83	22.088602004351614	28.07192773973576	27.71958264778159	22.119887608131044
84-85	22.198636620377517	28.0385036269664	27.674671182834583	22.088188569821504
86-87	22.03661479227737	28.092966850832518	27.75109832578807	22.119320031102045
88-89	22.097516974900582	28.064501155452966	27.740713041798987	22.097268827847472
90-91	22.05897447203743	28.134289595940842	27.746489998011125	22.060245934010606
92-93	22.058694505834673	28.148699976963666	27.745717881713233	22.046887635488428
94-95	21.92638558550042	28.211241429640673	27.7813040040086	22.08106898085031
96-97	21.91377378498151	28.22232626747958	27.846286935411698	22.01761301212721
98-99	22.10798056032275	28.840333472079298	27.91095734510857	21.14072862248938
100-101	22.898459301068165	31.483440905971655	25.091907677307805	20.526192115652375
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4317.0
1	3392.0
2	3888.5
3	5719.0
4	7701.5
5	12501.0
6	16860.5
7	20233.5
8	22477.5
9	21865.0
10	19087.5
11	16130.5
12	16659.5
13	21620.5
14	34054.5
15	50952.5
16	63892.5
17	70879.5
18	73346.0
19	72503.0
20	70282.5
21	69125.5
22	72088.5
23	82382.0
24	100848.0
25	126650.5
26	157117.0
27	188493.0
28	220029.0
29	252002.5
30	282230.5
31	314797.0
32	350177.0
33	384784.0
34	420027.0
35	453537.5
36	490794.0
37	524114.0
38	547722.5
39	570483.0
40	595839.5
41	620663.5
42	637802.0
43	652435.0
44	666988.0
45	677320.0
46	680724.5
47	682430.5
48	680636.0
49	669169.5
50	655422.5
51	635552.0
52	605871.5
53	574738.0
54	542310.0
55	503636.5
56	462436.5
57	418388.0
58	370954.5
59	324066.0
60	275219.5
61	226485.0
62	181360.5
63	141367.0
64	106624.5
65	79269.5
66	59801.5
67	46368.5
68	35788.5
69	26366.0
70	19460.0
71	14397.0
72	10453.5
73	7382.0
74	5219.0
75	3706.5
76	2607.5
77	1777.0
78	1214.5
79	858.0
80	632.0
81	462.5
82	332.5
83	229.0
84	156.0
85	115.5
86	94.5
87	77.0
88	57.5
89	38.5
90	22.5
91	14.5
92	11.0
93	7.0
94	4.5
95	3.5
96	3.0
97	3.0
98	1.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.631154809541281E-4
3	0.0
4	0.0013891086489243112
5	2.6828278627293565E-4
6	3.3982486261238513E-4
7	0.0
8	0.0
9	0.0
10-11	2.3549266795068794E-4
12-13	4.620425763589447E-4
14-15	2.9809198474770627E-6
16-17	0.0
18-19	8.197529580561922E-4
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.878102411078915E-5
28-29	0.0
30-31	3.885025944502106E-5
32-33	7.934190649080268E-4
34-35	7.112078220376777E-4
36-37	3.4018871803554444E-4
38-39	0.001545215733888571
40-41	3.038813457604192E-6
42-43	1.2218481601960235E-5
44-45	6.1473915818357366E-6
46-47	1.2383125354099339E-5
48-49	0.0
50-51	6.275224441246172E-6
52-53	1.2625346411887597E-5
54-55	0.002759272027198738
56-57	0.0025598114649321648
58-59	0.0011293346542580567
60-61	1.2635597374271028E-4
62-63	6.524300343817579E-6
64-65	7.225328164552514E-5
66-67	3.308208701257142E-5
68-69	1.4003137636373056E-4
70-71	0.0
72-73	0.0
74-75	9.564727689128312E-5
76-77	6.88861414034091E-6
78-79	2.986273904514934E-4
80-81	0.0
82-83	9.197477952495663E-5
84-85	1.7858556872163773E-5
86-87	0.0012663774900282694
88-89	0.0
90-91	5.9137731221598875E-5
92-93	1.8747011960631123E-5
94-95	1.3341658528254964E-4
96-97	1.9477642354491687E-5
98-99	1.2248293486096227E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	451.0
20-21	1477.0
22-23	3000.0
24-25	5787.0
26-27	9019.0
28-29	15646.0
30-31	29838.0
32-33	35573.0
34-35	48271.0
36-37	69038.0
38-39	81128.0
40-41	83017.0
42-43	96102.0
44-45	114967.0
46-47	113968.0
48-49	105726.0
50-51	96600.0
52-53	92330.0
54-55	99758.0
56-57	104874.0
58-59	107111.0
60-61	106790.0
62-63	102532.0
64-65	107814.0
66-67	115958.0
68-69	120787.0
70-71	120510.0
72-73	118127.0
74-75	122861.0
76-77	114051.0
78-79	126587.0
80-81	135502.0
82-83	135228.0
84-85	137996.0
86-87	151874.0
88-89	169769.0
90-91	187838.0
92-93	209412.0
94-95	256932.0
96-97	423690.0
98-99	1609050.0
100-101	1.0886357E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.89237199759076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.85169350547018	71.94383565168292
2	8.04898287146149	13.183005990239213
3	2.1949531582608093	5.392497616607426
4	0.8583886953879464	2.811819454449453
5	0.4170996294574622	1.7078639007793883
6	0.20750481008141192	1.0195836659085844
7	0.1234730864521607	0.707805274920172
8	0.07978281582237438	0.5226883225872921
9	0.05425755524641705	0.39989519081274766
>10	0.15941942002705012	1.9641644118929311
>50	0.003585351402616829	0.19339349620239382
>100	8.050532378653239E-4	0.10056661303577245
>500	2.7024438664446497E-5	0.019729737811837657
>1k	2.7023253598518746E-5	0.03315067306995645
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1923679389908251E-5	0.0	0.0	0.0
3	0.0	1.1923679389908251E-5	0.0	0.0	0.0
4	0.0	2.3847358779816502E-5	0.0	0.0	0.0
5	0.0	2.3847358779816502E-5	0.0	0.0	0.0
6	4.173287786467888E-5	2.3847358779816502E-5	0.0	0.0	0.0
7	4.173287786467888E-5	2.3847358779816502E-5	0.0	1.7885519084862376E-5	0.0
8	4.173287786467888E-5	2.9809198474770627E-5	0.0	1.7885519084862376E-5	0.0
9	4.173287786467888E-5	8.942759542431188E-5	0.0	3.577103816972475E-5	0.0
10-11	4.471379771215594E-5	8.942759542431188E-5	0.0	3.577103816972475E-5	0.0
12-13	5.365655725458713E-5	9.538943511926601E-5	0.0	4.173287786467888E-5	0.0
14-15	6.856115649197244E-5	1.43084152678899E-4	0.0	4.7694717559633004E-5	0.0
16-17	8.644667557683482E-5	1.5500783206880725E-4	0.0	4.7694717559633004E-5	5.9618396949541255E-6
18-19	8.942759542431188E-5	1.758742710011467E-4	0.0	5.365655725458713E-5	5.9618396949541255E-6
20-21	8.942759542431188E-5	1.9674070993348615E-4	0.0	5.9618396949541255E-5	5.9618396949541255E-6
22-23	8.942759542431188E-5	2.2953082825573383E-4	0.0	6.259931679701832E-5	5.9618396949541255E-6
24-25	8.942759542431188E-5	2.503972671880733E-4	0.0	1.0433219466169719E-4	5.9618396949541255E-6
26-27	8.942759542431188E-5	2.563591068830274E-4	0.0	1.6693151145871549E-4	5.9618396949541255E-6
28-29	8.942759542431188E-5	2.563591068830274E-4	0.0	5.007945343761466E-4	5.9618396949541255E-6
30-31	8.942759542431188E-5	2.7424462596788976E-4	0.0	0.0015262309619082561	5.9618396949541255E-6
32-33	8.942759542431188E-5	2.7424462596788976E-4	0.0	0.0032193934352752274	5.9618396949541255E-6
34-35	8.942759542431188E-5	3.487676221548163E-4	0.0	0.005258342610949538	1.1923679389908251E-5
36-37	8.942759542431188E-5	4.2329061834174293E-4	0.0	0.008397251210342885	1.4904599237385314E-5
38-39	9.240851527178894E-5	4.2329061834174293E-4	0.0	0.01344692943196903	1.7885519084862376E-5
40-41	9.538943511926601E-5	4.5906165651146767E-4	0.0	0.018830470676512605	1.7885519084862376E-5
42-43	1.0135127481422013E-4	4.709853359013759E-4	0.0	0.02345089644010205	1.7885519084862376E-5
44-45	1.0135127481422013E-4	5.455083320883025E-4	0.0	0.028437975344931178	1.7885519084862376E-5
46-47	1.0135127481422013E-4	6.081076488853208E-4	0.0	0.03394373430322131	1.7885519084862376E-5
48-49	1.0135127481422013E-4	6.081076488853208E-4	0.0	0.03950016889891855	1.7885519084862376E-5
50-51	1.1327495420412838E-4	6.617642061399079E-4	0.0	0.04516391660912498	1.7885519084862376E-5
52-53	1.1923679389908251E-4	6.67726045834862E-4	0.0	0.05039245002159974	1.7885519084862376E-5
54-55	1.1923679389908251E-4	6.826306450722474E-4	0.0	0.055767048506600886	2.3847358779816502E-5
56-57	1.1923679389908251E-4	7.034970840045868E-4	0.0	0.061535128411469006	2.9809198474770627E-5
58-59	1.1923679389908251E-4	7.034970840045868E-4	0.0	0.06680837562165592	3.279011832224769E-5
60-61	1.281795534415137E-4	7.064780038520638E-4	0.0	0.07203988995397817	3.577103816972475E-5
62-63	1.43084152678899E-4	7.15420763394495E-4	0.0	0.0770388925381972	3.577103816972475E-5
64-65	1.43084152678899E-4	7.988865191238528E-4	0.0	0.08179345969492313	3.577103816972475E-5
66-67	1.460650725263761E-4	8.10810198513761E-4	0.0	0.08684015699670179	3.577103816972475E-5
68-69	1.4904599237385314E-4	8.316766374461005E-4	0.0	0.09189877797787036	3.577103816972475E-5
70-71	1.5500783206880728E-4	8.406193969885317E-4	0.0	0.09647150902390017	3.577103816972475E-5
72-73	1.7885519084862375E-4	8.495621565309629E-4	0.0	0.09982802477215935	5.365655725458713E-5
74-75	1.7885519084862375E-4	8.853331947006876E-4	0.0	0.10271653610436463	5.365655725458713E-5
76-77	1.7885519084862375E-4	9.360088321077977E-4	0.0	0.10491943587165017	5.365655725458713E-5
78-79	1.8481703054357788E-4	9.598561908876142E-4	0.0	0.10719983955497013	5.365655725458713E-5
80-81	1.8481703054357788E-4	9.628371107350913E-4	0.0	0.10831768449777403	5.365655725458713E-5
82-83	1.8481703054357788E-4	9.717798702775224E-4	0.0	0.1088154981123027	5.365655725458713E-5
84-85	1.8481703054357788E-4	9.896653893623848E-4	0.0	0.10928350252835659	5.365655725458713E-5
86-87	1.8481703054357788E-4	0.0010135127481422012	0.0	0.10950110967722242	5.365655725458713E-5
88-89	1.8481703054357788E-4	0.001049283786311926	0.0	0.109554766234477	7.15420763394495E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	15880	0.0	47.27141	1
GTATCAA	27285	0.0	34.44615	1
TCAACGC	31035	0.0	29.20241	4
ATCAACG	31185	0.0	29.075836	3
CAACGCA	32000	0.0	28.32169	5
AACGCAG	33200	0.0	27.363585	6
TATCAAC	34165	0.0	26.897078	2
ACGCAGA	39035	0.0	23.262213	7
GTACATG	40690	0.0	23.248127	1
TACATGG	40095	0.0	23.12561	2
CGCAGAG	39530	0.0	22.937836	8
ACATGGG	40600	0.0	21.978926	3
CATGGGG	29865	0.0	19.6471	4
GCAGAGT	49280	0.0	18.311148	9
AGAGTAC	42620	0.0	18.032425	10-11
GAGTACT	29500	0.0	17.821375	12-13
CAGAGTA	47640	0.0	17.189163	10-11
ATGGGGG	19220	0.0	15.649856	5
GAGTACA	28590	0.0	15.567811	1
AGTACTT	33405	0.0	15.196514	12-13
>>END_MODULE