FastQCFastQC Report
Fri 27 May 2016
SRR2138818_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138818_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12310109
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124300.047.058771
GTATCAA209000.032.5561451
TCAACGC239950.027.477574
ATCAACG240350.027.4501783
CAACGCA244300.027.04095
AACGCAG254350.026.0909166
TATCAAC267150.025.0672232
GTACATG324300.023.8459991
TACATGG317350.023.7176862
ACATGGG319350.022.827943
ACGCAGA298900.022.130217
CGCAGAG303300.021.7808068
CATGGGG236000.020.2651424
GCAGAGT374950.017.550349
GAGTACT221000.017.54442612-13
AGAGTAC326700.017.377710-11
CAGAGTA362950.016.89819110-11
ATGGGGG159300.016.8199865
GAGTACA222750.016.1614571
GTACTTT258550.014.95526514-15
Produced by FastQC (version 0.11.5)