##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138818_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12310109
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.442650020401935	34.0	31.0	34.0	31.0	34.0
2	32.64122998423491	34.0	31.0	34.0	31.0	34.0
3	32.72241082511942	34.0	31.0	34.0	31.0	34.0
4	36.17956242304597	37.0	35.0	37.0	35.0	37.0
5	35.99394172707975	37.0	35.0	37.0	35.0	37.0
6	36.05653743602108	37.0	35.0	37.0	35.0	37.0
7	36.05709957564145	37.0	35.0	37.0	35.0	37.0
8	36.040950490365276	37.0	35.0	37.0	35.0	37.0
9	37.90502334301021	39.0	38.0	39.0	35.0	39.0
10-11	37.77761882530854	39.0	37.5	39.0	35.0	39.0
12-13	37.780696092942804	39.0	38.0	39.0	35.0	39.0
14-15	39.1099563375109	40.0	38.5	41.0	36.0	41.0
16-17	39.08151775910352	40.0	38.0	41.0	36.0	41.0
18-19	39.14420205377547	40.0	38.5	41.0	36.0	41.0
20-21	39.16078190071711	40.0	39.0	41.0	36.0	41.0
22-23	39.120896275178374	40.0	39.0	41.0	36.0	41.0
24-25	39.09000108768235	40.0	39.0	41.0	36.0	41.0
26-27	38.999519395620126	40.0	39.0	41.0	35.5	41.0
28-29	38.93613953131028	40.0	38.0	41.0	35.0	41.0
30-31	38.80596195176159	40.0	38.0	41.0	35.0	41.0
32-33	38.677432873895214	40.0	38.0	41.0	35.0	41.0
34-35	38.6388091779982	40.0	38.0	41.0	35.0	41.0
36-37	38.58985292067572	40.0	38.0	41.0	35.0	41.0
38-39	38.51907424116855	40.0	38.0	41.0	34.5	41.0
40-41	38.4319504426729	40.0	38.0	41.0	34.0	41.0
42-43	38.44761300035899	40.0	38.0	41.0	34.0	41.0
44-45	38.385362944302756	40.0	38.0	41.0	34.0	41.0
46-47	38.333179144791146	40.0	38.0	41.0	34.0	41.0
48-49	38.286705685703595	40.0	38.0	41.0	34.0	41.0
50-51	38.224344919896716	40.0	38.0	41.0	34.0	41.0
52-53	37.96926062654145	40.0	37.0	41.0	33.0	41.0
54-55	37.816900329432315	40.0	37.0	41.0	33.0	41.0
56-57	37.71932485233012	39.0	36.5	41.0	33.0	41.0
58-59	37.58091873921454	39.0	36.0	41.0	33.0	41.0
60-61	37.39649913992935	39.0	36.0	40.5	32.5	41.0
62-63	37.1407624764845	39.0	35.0	40.0	32.0	41.0
64-65	36.900026365752055	38.5	35.0	40.0	32.0	41.0
66-67	36.63705977401687	38.0	35.0	40.0	31.0	41.0
68-69	36.39875406648693	37.0	35.0	40.0	31.0	41.0
70-71	36.0944576630796	37.0	35.0	39.5	31.0	41.0
72-73	35.81116042475988	36.0	34.5	39.0	31.0	41.0
74-75	35.369676923182354	36.0	34.0	39.0	30.5	40.0
76-77	33.45839674291911	34.5	32.0	36.5	27.5	39.0
78-79	34.642314535730755	35.0	34.0	37.0	30.0	39.0
80-81	34.622832159098614	35.0	34.0	37.0	30.0	39.0
82-83	34.3572628751006	35.0	34.0	36.0	30.0	39.0
84-85	34.07932177523011	35.0	34.0	36.0	30.0	37.0
86-87	33.85041450495723	35.0	34.0	36.0	30.0	37.0
88-89	33.71322492276107	35.0	34.0	35.0	30.0	36.5
90-91	33.544714564365776	35.0	33.0	35.0	30.0	36.0
92-93	33.50806812193575	35.0	33.0	35.0	30.0	36.0
94-95	33.37843078996693	35.0	33.0	35.0	30.0	36.0
96-97	33.403502591462555	35.0	33.0	35.0	30.0	35.5
98-99	33.506061596539624	35.0	33.0	35.0	30.0	35.0
100-101	32.22811242003274	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	14.0
20	44.0
21	230.0
22	981.0
23	3463.0
24	9133.0
25	20215.0
26	37654.0
27	61775.0
28	91547.0
29	127329.0
30	170342.0
31	223696.0
32	295474.0
33	405230.0
34	595493.0
35	933055.0
36	1678934.0
37	3025465.0
38	3378356.0
39	1234747.0
40	16931.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.12984629136915	20.110179365592945	13.219582377377812	24.540391965660096
2	14.816362791225135	21.17256424016355	41.19184453457188	22.81922843403944
3	18.33007577677695	26.057763545261352	30.262424206975886	25.34973647098581
4	12.43898787438202	17.544993510588462	38.896241957691004	31.119776657338516
5	12.998171739508704	38.02192872789755	35.93410008820427	13.045799444389475
6	30.406325111429332	38.07005888849788	18.56178580677697	12.961830193295814
7	26.529570128095536	32.30698444668524	23.673039775683545	17.490405649535678
8	24.28213267648564	37.41017240383493	21.339088061689786	16.96860685798964
9	25.752932000845806	16.086721896613586	21.256456786857044	36.90388931568356
10-11	24.34896085603145	26.617454265329993	29.23382844269813	19.79975643594043
12-13	24.869343093414038	24.208766729021082	29.3112981450255	21.61059203253938
14-15	21.903875480325077	25.589945103674665	26.71311526847331	25.793064147526945
16-17	21.76529418476079	28.628539071853666	27.425355879651786	22.180810863733754
18-19	21.798937767326024	28.064065070423016	28.841227157289996	21.29577000496096
20-21	22.491868819362402	27.373810421087803	28.66849535809649	21.46582540145331
22-23	22.215677199991017	27.306994071417673	28.54502287212429	21.93230585646703
24-25	21.987951831703977	27.355372129152478	28.65747821027106	21.99919782887249
26-27	22.0252640646623	27.54095497122385	28.527587253335323	21.906193710778524
28-29	21.931211696290546	27.650690096074793	28.521338652756977	21.896759554877683
30-31	22.200462598541826	27.52760205368246	28.41895711771049	21.852978230065222
32-33	21.98541558505745	27.689663938600116	28.396605287379955	21.92831518896248
34-35	22.05711690853379	27.67482824203254	28.454116701168246	21.81393814826542
36-37	22.09689725074635	27.622079827214257	28.37354350581246	21.907479416226938
38-39	21.954621714441917	27.749226360008656	28.447041410119144	21.849110515430283
40-41	22.171879943561613	27.588292673109716	28.339881400883815	21.89994598244486
42-43	22.050069440803068	27.707309369020024	28.210203318202005	22.032417871974904
44-45	22.174188669571365	27.781182543733323	28.056629163644015	21.987999623051298
46-47	22.176879127430013	27.771227347993783	27.87002537130176	22.181868153274444
48-49	22.146759313773117	27.857875615318473	27.838031855968453	22.157333214939957
50-51	22.083044087601014	27.9802797005691	27.7907256369471	22.145950574882786
52-53	22.279324485377156	27.880898797792803	27.633766739452593	22.206009977377448
54-55	22.246984509819924	27.791449111655858	27.73078823592398	22.230778142600233
56-57	22.237578945999527	27.90539988823199	27.703732674697672	22.15328849107081
58-59	22.189874609491255	27.974084763282804	27.707878519362012	22.12816210786393
60-61	22.28632522261823	27.895237813396374	27.669253224081885	22.149183739903506
62-63	22.261085733085345	27.90299157382028	27.641529005961107	22.194393687133267
64-65	22.236621502751884	27.90527083606748	27.661740147109448	22.196367514071184
66-67	22.27825357014857	27.946813847729413	27.51896031291374	22.255972269208275
68-69	22.220537707844016	27.95770319302207	27.586070065739634	22.235689033394276
70-71	22.204245691099374	27.76929980297298	27.620326208218632	22.406128297709014
72-73	22.263786124687915	27.800771045547435	27.641845408828107	22.293597420936546
74-75	22.144138733092905	27.926026863071655	27.588511593874564	22.34132280996087
76-77	22.246372018445406	27.889062004361737	27.522828630649098	22.341737346543756
78-79	22.192785762544727	27.912233123503515	27.51753620926613	22.377444904685625
80-81	22.156493501025775	27.9146876280742	27.566195298603496	22.362623572296524
82-83	22.249519389819145	27.891225047384243	27.628347501165447	22.230908061631162
84-85	22.341202305060484	27.895396761896706	27.5268895484394	22.23651138460341
86-87	22.18835835095411	27.914490710560585	27.61900320011551	22.278147738369793
88-89	22.213966703575	27.9126330450907	27.564570444897264	22.308829806437032
90-91	22.27179044147182	27.9320803299644	27.56604636700756	22.230082861556223
92-93	22.247578614279124	27.88643153800514	27.625230404113655	22.240759443602084
94-95	22.099405365331563	27.941008934510098	27.681691428121113	22.277894272037223
96-97	22.129511581643577	27.98145615100337	27.69189188422752	22.197140383125532
98-99	22.40104299896142	28.581768756378345	27.711632422665446	21.305555821994798
100-101	23.156231229617624	31.155909989424558	24.85920322908171	20.828655551876107
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2634.0
1	2297.5
2	3164.5
3	4711.5
4	6495.0
5	10648.0
6	14095.5
7	16311.0
8	17556.0
9	16815.0
10	14710.5
11	12553.0
12	13157.0
13	17087.0
14	26497.0
15	39220.0
16	48994.0
17	54191.5
18	55830.5
19	54792.5
20	52998.0
21	52739.5
22	55461.0
23	63088.5
24	76130.0
25	94144.5
26	116131.5
27	139358.0
28	162823.5
29	187113.5
30	209079.5
31	230626.0
32	255187.5
33	281057.0
34	306603.0
35	332498.0
36	361934.5
37	386661.0
38	404143.0
39	419437.5
40	434625.5
41	449677.0
42	461187.0
43	472579.0
44	486329.5
45	497878.5
46	503486.5
47	505767.0
48	505104.0
49	499344.0
50	490560.5
51	478765.5
52	461044.0
53	441807.0
54	420714.0
55	393665.0
56	362951.5
57	328613.5
58	292309.0
59	255454.5
60	215686.0
61	176897.0
62	142498.5
63	111815.5
64	84744.0
65	62916.0
66	47374.0
67	36343.0
68	27826.0
69	20713.0
70	15260.0
71	11115.0
72	8007.0
73	5772.0
74	4094.5
75	2782.0
76	1895.5
77	1276.0
78	884.5
79	625.5
80	447.5
81	327.5
82	246.5
83	169.0
84	117.5
85	82.0
86	61.5
87	50.0
88	34.0
89	21.0
90	15.0
91	11.0
92	9.0
93	6.0
94	3.0
95	2.0
96	2.0
97	2.0
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0010641660443461549
3	0.0021851959231230203
4	4.5491067544568457E-4
5	3.818000311776281E-4
6	5.280213197137409E-4
7	0.0
8	0.0
9	0.0
10-11	4.06170245933647E-6
12-13	8.245255992453031E-4
14-15	7.026745254652091E-4
16-17	1.0966596640208466E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.689753586932424E-5
28-29	1.8706274084327883E-4
30-31	7.32951397789215E-5
32-33	0.0011468106135730626
34-35	0.0019602555125536156
36-37	0.0013639409908184996
38-39	7.602268483862244E-4
40-41	3.742266294846176E-5
42-43	5.858743926313406E-5
44-45	6.493257616327659E-4
46-47	3.4025740132152574E-5
48-49	0.0
50-51	0.0
52-53	0.0010498907198808788
54-55	0.003778271044739337
56-57	0.006563750247356962
58-59	0.00468274137313051
60-61	2.878375243526287E-4
62-63	4.5347124300775684E-5
64-65	1.1427987360463132E-4
66-67	1.7057867339674834E-4
68-69	0.0
70-71	1.1270212598003304E-4
72-73	9.480455850654759E-6
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	1.016119258665808E-5
88-89	2.774668692996936E-4
90-91	9.365465760741695E-5
92-93	5.115670316038201E-4
94-95	2.9444693951048034E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	254.0
20-21	768.0
22-23	1610.0
24-25	3516.0
26-27	6021.0
28-29	12475.0
30-31	26812.0
32-33	31680.0
34-35	41748.0
36-37	64108.0
38-39	77865.0
40-41	75358.0
42-43	85095.0
44-45	101126.0
46-47	101423.0
48-49	95693.0
50-51	86726.0
52-53	82314.0
54-55	88114.0
56-57	92741.0
58-59	93533.0
60-61	92726.0
62-63	88274.0
64-65	90835.0
66-67	96396.0
68-69	99910.0
70-71	101012.0
72-73	95861.0
74-75	97130.0
76-77	94764.0
78-79	103522.0
80-81	107111.0
82-83	103295.0
84-85	102521.0
86-87	107881.0
88-89	118667.0
90-91	127799.0
92-93	135494.0
94-95	161830.0
96-97	251063.0
98-99	1075444.0
100-101	7889594.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.61692001661582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.63827296907648	71.52765915311461
2	8.145372956945412	13.296005062650384
3	2.2649282079745015	5.545693931808956
4	0.8693945584355798	2.8382922455487103
5	0.4182296935464097	1.7067309723375537
6	0.23716860994919825	1.1614182881205404
7	0.1261853249649478	0.7209200302454375
8	0.08482472526384198	0.5538506253832322
9	0.05044788854065592	0.37056611556268776
>10	0.16159338335853904	1.9814941972206561
>50	0.002939299598551078	0.15616712241884648
>100	5.513924447619564E-4	0.06346396149573873
>500	3.639667374303855E-5	0.021417853349392085
>1k	5.45932274107656E-5	0.05632044074320539
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.123404918672937E-6
2	0.0	4.874042951203763E-5	0.0	0.0	8.123404918672937E-6
3	0.0	4.874042951203763E-5	0.0	0.0	8.123404918672937E-6
4	0.0	1.4622128853611287E-4	0.0	8.123404918672937E-6	8.123404918672937E-6
5	0.0	1.4622128853611287E-4	0.0	8.123404918672937E-6	8.123404918672937E-6
6	0.0	1.4622128853611287E-4	0.0	8.123404918672937E-6	1.6246809837345875E-5
7	0.0	1.4622128853611287E-4	0.0	8.123404918672937E-6	1.6246809837345875E-5
8	0.0	1.6246809837345877E-4	0.0	8.123404918672937E-6	3.249361967469175E-5
9	0.0	2.59948957397534E-4	0.0	2.4370214756018814E-5	3.249361967469175E-5
10-11	0.0	2.59948957397534E-4	0.0	2.4370214756018814E-5	3.249361967469175E-5
12-13	0.0	2.59948957397534E-4	0.0	4.061702459336469E-5	3.249361967469175E-5
14-15	0.0	3.9398513855563747E-4	0.0	4.061702459336469E-5	3.6555322134028225E-5
16-17	0.0	4.305404606896657E-4	0.0	6.498723934938351E-5	4.061702459336469E-5
18-19	0.0	4.6709578282369394E-4	0.0	9.748085902407526E-5	4.061702459336469E-5
20-21	0.0	5.158362123357316E-4	0.0	1.1372766886142113E-4	4.061702459336469E-5
22-23	0.0	5.930085590631245E-4	0.0	1.421595860767764E-4	4.874042951203763E-5
24-25	0.0	6.701809057905173E-4	0.0	1.5028299099544934E-4	5.6863834430710564E-5
26-27	0.0	7.067362279245456E-4	0.0	2.3151704018217872E-4	5.6863834430710564E-5
28-29	0.0	7.229830377618914E-4	0.0	6.66119203331181E-4	5.6863834430710564E-5
30-31	8.123404918672937E-6	8.529575164606584E-4	0.0	0.0017871490821080463	6.092553689004703E-5
32-33	8.123404918672937E-6	8.529575164606584E-4	0.0	0.004094196079011161	6.49872393493835E-5
34-35	8.123404918672937E-6	9.666851853220796E-4	0.0	0.0066855622480678275	7.311064426805644E-5
36-37	8.123404918672937E-6	0.0010763511517241642	0.0	0.01022736679260923	7.311064426805644E-5
38-39	8.123404918672937E-6	0.001153523498451557	0.0	0.016393031125881988	7.311064426805644E-5
40-41	8.123404918672937E-6	0.0012956830845283337	0.0	0.02290394016819835	7.311064426805644E-5
42-43	8.123404918672937E-6	0.0013606703238777172	0.0	0.02878122362685822	7.311064426805644E-5
44-45	8.123404918672937E-6	0.0014743979927391382	0.0	0.03530025607409325	7.311064426805644E-5
46-47	8.123404918672937E-6	0.001567817149303877	0.0	0.042635690715654914	7.311064426805644E-5
48-49	8.123404918672937E-6	0.0015800022566818863	0.0	0.0493943636079908	9.748085902407526E-5
50-51	8.123404918672937E-6	0.001827766106701411	0.0	0.05607992585605863	9.748085902407526E-5
52-53	8.123404918672937E-6	0.001844012916538757	0.0	0.062473045527054225	1.0966596640208466E-4
54-55	8.123404918672937E-6	0.0018602597263761029	0.0	0.06861027794311164	1.2591277623943053E-4
56-57	8.123404918672937E-6	0.0019008767509694675	0.0	0.07446319118701548	1.3403618115810347E-4
58-59	8.123404918672937E-6	0.0019130618583474768	0.0	0.08002366185384711	1.421595860767764E-4
60-61	8.123404918672937E-6	0.0019618022878595143	0.0	0.08527138143130983	1.5028299099544934E-4
62-63	8.123404918672937E-6	0.0019983576099935427	0.0	0.0907140627268207	1.6246809837345877E-4
64-65	8.123404918672937E-6	0.0021364554936109826	0.0	0.09568558653704853	1.6246809837345877E-4
66-67	8.123404918672937E-6	0.0021689491132856746	0.0	0.10108765080796603	1.6246809837345877E-4
68-69	8.123404918672937E-6	0.002237998055094394	0.0	0.1065303321034769	1.6246809837345877E-4
70-71	8.123404918672937E-6	0.0022664299723097496	0.0	0.1117861750858583	1.705915032921317E-4
72-73	8.123404918672937E-6	0.002278615079687759	0.0	0.11607127118045826	1.7871490821080464E-4
74-75	8.123404918672937E-6	0.00232329380674046	0.0	0.11968618636926773	1.7871490821080464E-4
76-77	8.123404918672937E-6	0.0024045278559271894	0.0	0.12293148663427757	1.7871490821080464E-4
78-79	8.123404918672937E-6	0.0024451448805205543	0.0	0.12584372729762183	1.7871490821080464E-4
80-81	8.123404918672937E-6	0.0024492065829798907	0.0	0.12700537420099206	1.9090001558881404E-4
82-83	8.123404918672937E-6	0.0024654533928172365	0.0	0.1274765316862751	2.1120852788549638E-4
84-85	8.123404918672937E-6	0.0024817002026545827	0.0	0.12786239341991204	2.1120852788549638E-4
86-87	8.123404918672937E-6	0.002502008714951265	0.0	0.12802079981582615	2.1527023034483285E-4
88-89	8.123404918672937E-6	0.0025669959543006484	0.0	0.12808984875763488	2.1933193280416932E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12430	0.0	47.05877	1
GTATCAA	20900	0.0	32.556145	1
TCAACGC	23995	0.0	27.47757	4
ATCAACG	24035	0.0	27.450178	3
CAACGCA	24430	0.0	27.0409	5
AACGCAG	25435	0.0	26.090916	6
TATCAAC	26715	0.0	25.067223	2
GTACATG	32430	0.0	23.845999	1
TACATGG	31735	0.0	23.717686	2
ACATGGG	31935	0.0	22.82794	3
ACGCAGA	29890	0.0	22.13021	7
CGCAGAG	30330	0.0	21.780806	8
CATGGGG	23600	0.0	20.265142	4
GCAGAGT	37495	0.0	17.55034	9
GAGTACT	22100	0.0	17.544426	12-13
AGAGTAC	32670	0.0	17.3777	10-11
CAGAGTA	36295	0.0	16.898191	10-11
ATGGGGG	15930	0.0	16.819986	5
GAGTACA	22275	0.0	16.161457	1
GTACTTT	25855	0.0	14.955265	14-15
>>END_MODULE