FastQCFastQC Report
Fri 27 May 2016
SRR2138817_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138817_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14907046
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203950.041.811591
GTATCAA333550.032.0238651
TCAACGC397850.026.2943694
ATCAACG398600.026.192163
CAACGCA406800.025.6844865
TATCAAC419450.025.0822352
AACGCAG421950.024.852976
GTACATG503150.023.9713611
TACATGG496850.023.7301852
ACATGGG507100.022.7578373
ACGCAGA481200.021.7489347
CGCAGAG483800.021.5533268
CATGGGG334050.020.016094
GCAGAGT569750.018.2947039
AGAGTAC494350.017.81496810-11
GAGTACT305550.017.53463412-13
GAGTACA350050.017.3003481
CAGAGTA558050.016.77109110-11
AGTACAT359950.016.3072072
ATGGGGG214700.015.827575
Produced by FastQC (version 0.11.5)