##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138817_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14907046
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39811502560601	33.0	31.0	34.0	31.0	34.0
2	32.59212905091995	34.0	31.0	34.0	31.0	34.0
3	32.669497430946414	34.0	31.0	34.0	31.0	34.0
4	36.14203531672204	37.0	35.0	37.0	35.0	37.0
5	35.9567718513782	37.0	35.0	37.0	35.0	37.0
6	36.02574769005207	37.0	35.0	37.0	35.0	37.0
7	36.02474514400774	37.0	35.0	37.0	35.0	37.0
8	36.006648936348626	37.0	35.0	37.0	35.0	37.0
9	37.85679852332917	39.0	38.0	39.0	35.0	39.0
10-11	37.723947823063	39.0	37.0	39.0	35.0	39.0
12-13	37.728419668122044	39.0	37.0	39.0	35.0	39.0
14-15	39.026707538166846	40.0	38.0	41.0	36.0	41.0
16-17	38.9946551114151	40.0	38.0	41.0	36.0	41.0
18-19	39.06840986470425	40.0	38.5	41.0	36.0	41.0
20-21	39.08988860932511	40.0	39.0	41.0	36.0	41.0
22-23	39.04987254569092	40.0	39.0	41.0	36.0	41.0
24-25	39.01730085527793	40.0	38.5	41.0	36.0	41.0
26-27	38.92207692681419	40.0	38.0	41.0	35.0	41.0
28-29	38.856654871547306	40.0	38.0	41.0	35.0	41.0
30-31	38.72296650119162	40.0	38.0	41.0	35.0	41.0
32-33	38.589324717237986	40.0	38.0	41.0	35.0	41.0
34-35	38.54996738575906	40.0	38.0	41.0	35.0	41.0
36-37	38.50294852215228	40.0	38.0	41.0	34.0	41.0
38-39	38.439018758020765	40.0	38.0	41.0	34.0	41.0
40-41	38.353221494648025	40.0	38.0	41.0	34.0	41.0
42-43	38.37487449852559	40.0	38.0	41.0	34.0	41.0
44-45	38.31781410069043	40.0	38.0	41.0	34.0	41.0
46-47	38.27040650553222	40.0	38.0	41.0	34.0	41.0
48-49	38.23443690367021	40.0	38.0	41.0	34.0	41.0
50-51	38.17885415912558	40.0	37.5	41.0	33.5	41.0
52-53	37.93320291397248	40.0	37.0	41.0	33.0	41.0
54-55	37.78907457388994	40.0	37.0	41.0	33.0	41.0
56-57	37.698198618098274	39.0	37.0	41.0	33.0	41.0
58-59	37.568775506703744	39.0	36.0	41.0	33.0	41.0
60-61	37.395232092344514	39.0	36.0	40.5	32.0	41.0
62-63	37.15414908543712	39.0	35.0	40.0	32.0	41.0
64-65	36.9218727833083	38.5	35.0	40.0	32.0	41.0
66-67	36.668066597955125	38.0	35.0	40.0	31.0	41.0
68-69	36.43349508034639	37.5	35.0	40.0	31.0	41.0
70-71	36.13265843101611	37.0	35.0	39.5	31.0	41.0
72-73	35.85048918157811	36.5	34.5	39.0	31.0	41.0
74-75	35.40251712479473	36.0	34.0	39.0	30.5	40.0
76-77	33.45780074553471	34.5	32.0	36.5	27.5	39.0
78-79	34.674211950599044	35.0	34.0	37.0	30.0	39.0
80-81	34.6544905226186	35.0	34.0	37.0	30.0	39.0
82-83	34.37991597031911	35.0	34.0	36.5	30.0	39.0
84-85	34.09204242963365	35.0	34.0	36.0	30.0	37.0
86-87	33.851349334480346	35.0	34.0	36.0	30.0	37.0
88-89	33.70373787396428	35.0	34.0	35.0	30.0	37.0
90-91	33.527966340518304	35.0	33.0	35.0	30.0	36.0
92-93	33.4849407377333	35.0	33.0	35.0	30.0	36.0
94-95	33.351319487186004	35.0	33.0	35.0	29.5	36.0
96-97	33.37455803646013	35.0	33.0	35.0	30.0	35.5
98-99	33.474895630647914	35.0	33.0	35.0	30.0	35.0
100-101	32.19727216712774	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	0.0
18	4.0
19	20.0
20	78.0
21	297.0
22	1301.0
23	4305.0
24	11271.0
25	24384.0
26	44600.0
27	73222.0
28	109827.0
29	153777.0
30	206328.0
31	272749.0
32	362199.0
33	499696.0
34	734601.0
35	1143765.0
36	2036017.0
37	3614681.0
38	4044375.0
39	1544471.0
40	25076.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.08860695807875	20.41446038336502	13.507766729907456	23.989165928648777
2	14.639024908909724	21.564837526745137	41.5769057514583	22.21923181288684
3	18.255020563512765	26.60819335249826	30.491503073474867	24.645283010514103
4	12.199868290038564	17.97235888623309	39.301745348174414	30.52602747555393
5	12.721214308920095	38.48545188324853	35.92991095916739	12.86342284866399
6	29.595945955827034	38.47126492529579	19.012951708362873	12.919837410514301
7	26.28770985210618	32.75485968179074	23.729315653819004	17.22811481228407
8	24.15938073847763	37.41586361241523	21.4932589595551	16.93149668955204
9	25.86242103230915	16.18015400234225	21.404596188943135	36.55282877640546
10-11	24.016521987860237	26.910274766747854	29.40517583499452	19.668027410397393
12-13	24.770087444302284	24.592277377264615	29.49810381656704	21.13953136186606
14-15	21.77191148740711	25.940010093284734	27.113396370187555	25.174682049120605
16-17	21.33348180916685	29.017632790977277	27.819446230980656	21.82943916887522
18-19	21.379691858467464	28.367997925276413	29.362520917960538	20.889789298295586
20-21	22.220537276013545	27.52899999828934	29.190549269767008	21.05991345593011
22-23	21.896532487670946	27.536790922976813	28.999732912702708	21.566943676649533
24-25	21.63107415554028	27.60300245484954	29.151621768904867	21.614301620705316
26-27	21.615011488332556	27.741977860680727	29.102027140168264	21.540983510818453
28-29	21.51738987072413	27.953543623580835	29.017742628751137	21.511323876943894
30-31	21.77570600550346	27.7883139140003	28.9524479218123	21.483532158683932
32-33	21.583298993703252	27.8629217728949	28.931454815601075	21.622324417800773
34-35	21.691635693285555	27.953995397834504	28.914834223683755	21.439534685196186
36-37	21.66906831522957	27.921942212858603	28.87626740818508	21.532722063726748
38-39	21.561943884361472	28.014335767442144	28.890279688749708	21.533440659446672
40-41	21.80036181256973	27.91905665440062	28.757875406043183	21.522706126986467
42-43	21.6973838056408	27.977282961401563	28.59344065199015	21.73189258096749
44-45	21.754203356322403	28.10714083127338	28.43348625548792	21.705169556916292
46-47	21.796595412695254	28.129829021550645	28.21761629893752	21.855959266816573
48-49	21.791776714614155	28.207327561870272	28.149429138061173	21.8514665854544
50-51	21.754450213855133	28.317714343136135	28.09011426782771	21.837721175181024
52-53	21.91274959483589	28.219252990787396	27.949393578137467	21.91860383623925
54-55	21.87457314750054	28.15102698691852	28.021719136416255	21.95268072916468
56-57	21.90318650222381	28.22960758574322	27.961265914222423	21.905939997810545
58-59	21.867400112341205	28.29247581842872	27.95531002265832	21.88481404657176
60-61	21.96846687165358	28.227055936985284	27.942297729979494	21.862179461381643
62-63	21.887044979726852	28.255493560011253	27.93680213996294	21.920659320298956
64-65	21.89190089615588	28.245804956347666	27.92546124537455	21.936832902121907
66-67	21.954052605023637	28.24501137894745	27.769171912889828	22.03176410313909
68-69	21.91277870611925	28.234221110580776	27.876803854042254	21.976196329257725
70-71	21.90218005824927	28.071309917358167	27.937715780482282	22.088794243910282
72-73	21.913557990405163	28.108656487594825	27.937162406595935	22.04062311540408
74-75	21.89927257761238	28.17940388678769	27.8766713742851	22.044652161314826
76-77	21.90847526212119	28.130259308279413	27.848047359188556	22.11321807041084
78-79	21.93977100407855	28.08496058870172	27.84502089586744	22.13024751135229
80-81	21.92433954336992	28.105301755239743	27.86217659158324	22.1081821098071
82-83	21.985969501893997	28.058216595478786	27.93108324512385	22.02473065750337
84-85	22.07473442225754	28.055068768413133	27.827591633309485	22.04260517601984
86-87	21.954193485870192	28.022516570127515	27.928985273945646	22.09430467005665
88-89	21.98532961186675	28.01189457400336	27.89028600189943	22.112489812230464
90-91	22.04176626273574	27.95785146825031	27.91468655065177	22.08569571836218
92-93	22.01843270534915	27.91377915712696	27.975391854745606	22.092396282778285
94-95	21.928736899775487	27.925379178649614	28.01427617581873	22.131607745756167
96-97	21.948863745619178	27.93888414495039	28.05390190326496	22.058350206165475
98-99	22.167236482426155	28.532985181621996	28.06758820337569	21.23219013257616
100-101	22.92442231406861	31.062075888057212	25.23407425307871	20.779427544795467
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2491.0
1	2354.5
2	3647.5
3	5530.0
4	7965.5
5	13702.5
6	18573.0
7	21765.5
8	23720.0
9	23001.0
10	20454.5
11	17708.0
12	18689.5
13	24780.5
14	39104.5
15	58432.5
16	73168.0
17	80681.0
18	82504.0
19	80060.5
20	75906.5
21	72853.5
22	73992.0
23	83840.0
24	102447.5
25	127563.5
26	157677.5
27	187777.0
28	217835.0
29	249896.0
30	279791.0
31	309297.0
32	341030.5
33	372903.5
34	404603.5
35	436491.0
36	469317.5
37	497485.0
38	519108.0
39	539306.0
40	559388.0
41	575646.5
42	585397.5
43	594073.5
44	603983.0
45	613998.0
46	621970.0
47	626605.0
48	625012.5
49	613349.5
50	598227.0
51	576516.5
52	546130.5
53	516542.0
54	484939.5
55	447199.5
56	407199.0
57	365284.5
58	321588.5
59	277754.5
60	235227.5
61	193449.5
62	154105.5
63	120206.5
64	90849.5
65	68061.5
66	51189.0
67	38918.5
68	29369.5
69	20999.0
70	14838.0
71	10662.5
72	7700.0
73	5523.0
74	3944.0
75	2772.0
76	1967.0
77	1371.0
78	944.0
79	662.5
80	454.5
81	323.0
82	252.5
83	186.0
84	124.5
85	83.5
86	60.0
87	41.0
88	26.5
89	20.5
90	15.5
91	12.0
92	11.0
93	8.5
94	6.0
95	3.5
96	2.0
97	2.0
98	1.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	8.787790686364019E-4
3	0.002032595861044502
4	3.287036210930053E-4
5	4.360354157356193E-4
6	3.6895304408398553E-4
7	0.0
8	0.0
9	0.0
10-11	1.3416474330326747E-5
12-13	8.620084757234934E-4
14-15	7.043649023421542E-4
16-17	1.241023875555224E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.356363468789562E-5
28-29	1.914512644012496E-4
30-31	3.700232710999043E-5
32-33	0.0010286606093508892
34-35	0.0017458541799656938
36-37	0.0012405465256608238
38-39	6.876030741809801E-4
40-41	3.446226664393076E-5
42-43	4.1668860069161976E-5
44-45	6.34159443380388E-4
46-47	1.4160378249191717E-5
48-49	0.0
50-51	0.0
52-53	9.502129896955409E-4
54-55	0.003348614569469506
56-57	0.0061032711272771415
58-59	0.004377613051482899
60-61	2.1539337064551125E-4
62-63	2.2892848724284558E-5
64-65	1.0013980672478087E-4
66-67	1.7506940140114656E-4
68-69	0.0
70-71	1.0340708445912824E-4
72-73	8.04362314225988E-6
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	2.0824100946025616E-4
90-91	5.8464638540348446E-5
92-93	5.530896341174941E-4
94-95	3.070055884320294E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	333.0
20-21	1066.0
22-23	2129.0
24-25	4831.0
26-27	8915.0
28-29	16997.0
30-31	37430.0
32-33	44399.0
34-35	59099.0
36-37	88940.0
38-39	107896.0
40-41	107373.0
42-43	121794.0
44-45	145168.0
46-47	143711.0
48-49	133571.0
50-51	121206.0
52-53	114474.0
54-55	123119.0
56-57	128609.0
58-59	131422.0
60-61	129172.0
62-63	122596.0
64-65	127056.0
66-67	136516.0
68-69	141916.0
70-71	141204.0
72-73	134741.0
74-75	135205.0
76-77	134872.0
78-79	146812.0
80-81	149853.0
82-83	144652.0
84-85	143756.0
86-87	151416.0
88-89	163452.0
90-91	178124.0
92-93	183016.0
94-95	210046.0
96-97	307779.0
98-99	1256569.0
100-101	9025811.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.82617101913145
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.54887588806774	73.34164337856944
2	7.607307366903691	12.601682819325276
3	2.0780310648293403	5.163460690758685
4	0.7966355796902507	2.639290990533985
5	0.37353099980311155	1.5469071235319838
6	0.20869565325054196	1.0371277120247249
7	0.11582480917502794	0.671532781709239
8	0.07333755213757351	0.48594149083769067
9	0.05089658379433111	0.3794012238274941
>10	0.14262473822366506	1.7897235733219263
>50	0.003278981372159407	0.17990947653141115
>100	8.740152792713221E-4	0.10937509924381265
>500	7.231997724402952E-5	0.03929704751997499
>1k	1.44474960449844E-5	0.01470659226432507
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.708237165163373E-6	6.708237165163373E-6
2	0.0	1.3416474330326746E-5	0.0	6.708237165163373E-6	6.708237165163373E-6
3	0.0	1.3416474330326746E-5	0.0	6.708237165163373E-6	6.708237165163373E-6
4	0.0	6.708237165163374E-5	0.0	1.3416474330326746E-5	1.3416474330326746E-5
5	0.0	6.708237165163374E-5	0.0	1.3416474330326746E-5	1.3416474330326746E-5
6	0.0	6.708237165163374E-5	0.0	2.012471149549012E-5	3.354118582581687E-5
7	0.0	6.708237165163374E-5	0.0	2.012471149549012E-5	3.354118582581687E-5
8	0.0	7.37906088167971E-5	0.0	2.012471149549012E-5	4.024942299098024E-5
9	0.0	1.2074826897294071E-4	0.0	4.024942299098024E-5	4.024942299098024E-5
10-11	0.0	1.2074826897294071E-4	0.0	6.0374134486470356E-5	4.024942299098024E-5
12-13	0.0	1.2074826897294071E-4	0.0	6.708237165163374E-5	5.03117787387253E-5
14-15	0.0	1.5428945479875757E-4	0.0	7.043649023421542E-5	8.049884598196048E-5
16-17	0.0	1.5428945479875757E-4	0.0	7.37906088167971E-5	8.720708314712385E-5
18-19	0.0	1.8447652204199274E-4	0.0	8.049884598196048E-5	1.0397767606003228E-4
20-21	0.0	2.1466358928522793E-4	0.0	9.391532031228722E-5	1.1404003180777734E-4
22-23	0.0	2.549130122762082E-4	0.0	1.006235574774506E-4	1.3751886188584914E-4
24-25	0.0	2.851000795194434E-4	0.0	1.241023875555224E-4	1.6099769196392097E-4
26-27	0.0	2.951624352671884E-4	0.0	1.9453887778973783E-4	1.8112240345941107E-4
28-29	0.0	2.951624352671884E-4	0.0	7.613849182460429E-4	1.9453887778973783E-4
30-31	0.0	3.287036210930053E-4	0.0	0.002022533505296757	2.0460123353748287E-4
32-33	0.0	3.3205773967558697E-4	0.0	0.004165815279566455	2.1466358928522793E-4
34-35	0.0	4.159107042401291E-4	0.0	0.006627738319181413	2.3478830078071806E-4
36-37	0.0	4.762848387265995E-4	0.0	0.010364226420177411	2.4149653794588142E-4
38-39	0.0	4.964095502220896E-4	0.0	0.016200392753869548	2.549130122762082E-4
40-41	0.0	5.433672103782332E-4	0.0	0.02240551213164567	2.6162124944137155E-4
42-43	0.0	5.534295661259783E-4	0.0	0.027953224267235774	2.6162124944137155E-4
44-45	0.0	6.003872262821219E-4	0.0	0.03413486481493382	2.683294866065349E-4
46-47	0.0	6.305742935253571E-4	0.0	0.04070222899962876	2.851000795194434E-4
48-49	0.0	6.305742935253571E-4	0.0	0.04736686262321858	3.287036210930053E-4
50-51	0.0	6.641154793511739E-4	0.0	0.05385372796193155	3.287036210930053E-4
52-53	0.0	6.708237165163373E-4	0.0	0.060166179134350295	3.387659768407503E-4
54-55	0.0	6.808860722640824E-4	0.0	0.0665557750341684	3.521824511710771E-4
56-57	0.0	6.909484280118274E-4	0.0	0.0733512192824789	3.6559892550140383E-4
58-59	0.0	6.909484280118274E-4	0.0	0.07991522934859127	3.7901539983173057E-4
60-61	3.3541185825816864E-6	6.976566651769908E-4	0.0	0.08616730638652353	3.95785992744639E-4
62-63	6.708237165163373E-6	7.144272580898992E-4	0.0	0.09182905855392141	3.95785992744639E-4
64-65	6.708237165163373E-6	7.982802226544414E-4	0.0	0.09755453897438834	3.991401113272207E-4
66-67	6.708237165163373E-6	8.049884598196048E-4	0.0	0.10365568067610445	4.125565856575474E-4
68-69	6.708237165163373E-6	8.284672898976767E-4	0.0	0.10991111183261928	4.192648228227108E-4
70-71	6.708237165163373E-6	8.45237882810585E-4	0.0	0.1155124898655307	4.2932717857045586E-4
72-73	6.708237165163373E-6	8.5530023855833E-4	0.0	0.12033235826870059	4.3603541573561925E-4
74-75	6.708237165163373E-6	8.787790686364019E-4	0.0	0.12427344760323407	4.3938953431820095E-4
76-77	6.708237165163373E-6	8.955496615493103E-4	0.0	0.12810385102454236	4.4274365290078265E-4
78-79	6.708237165163373E-6	9.056120172970554E-4	0.0	0.13126678484791687	4.4274365290078265E-4
80-81	6.708237165163373E-6	9.089661358796371E-4	0.0	0.13264532758535796	4.595142458136911E-4
82-83	6.708237165163373E-6	9.156743730448005E-4	0.0	0.13326919364171813	4.997636688046712E-4
84-85	6.708237165163373E-6	9.257367287925454E-4	0.0	0.13392660088390418	5.098260245524163E-4
86-87	6.708237165163373E-6	9.425073217054539E-4	0.0	0.13413120211744164	5.098260245524163E-4
88-89	6.708237165163373E-6	0.0010095896933570876	0.0	0.1342117009634236	5.232424988827431E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20395	0.0	41.81159	1
GTATCAA	33355	0.0	32.023865	1
TCAACGC	39785	0.0	26.294369	4
ATCAACG	39860	0.0	26.19216	3
CAACGCA	40680	0.0	25.684486	5
TATCAAC	41945	0.0	25.082235	2
AACGCAG	42195	0.0	24.85297	6
GTACATG	50315	0.0	23.971361	1
TACATGG	49685	0.0	23.730185	2
ACATGGG	50710	0.0	22.757837	3
ACGCAGA	48120	0.0	21.748934	7
CGCAGAG	48380	0.0	21.553326	8
CATGGGG	33405	0.0	20.01609	4
GCAGAGT	56975	0.0	18.294703	9
AGAGTAC	49435	0.0	17.814968	10-11
GAGTACT	30555	0.0	17.534634	12-13
GAGTACA	35005	0.0	17.300348	1
CAGAGTA	55805	0.0	16.771091	10-11
AGTACAT	35995	0.0	16.307207	2
ATGGGGG	21470	0.0	15.82757	5
>>END_MODULE