FastQCFastQC Report
Fri 27 May 2016
SRR2138816_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138816_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18015164
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA230400.048.9100841
GTATCAA411650.036.288151
ATCAACG499750.028.9289323
TCAACGC502750.028.7726864
CAACGCA507350.028.4451545
AACGCAG525400.027.5241176
TATCAAC535950.027.344812
ACGCAGA607700.023.761937
TACATGG640200.023.6232172
CGCAGAG615550.023.4383438
GTACATG656850.023.4353281
ACATGGG649500.022.6810863
CATGGGG410050.020.1481884
GCAGAGT731350.019.5833429
AGAGTAC670350.018.2417510-11
GAGTACT425250.018.13403512-13
CAGAGTA715400.017.75623910-11
GTGGTAT121850.017.098021
ATGGGGG256950.016.6427575
TGGTATC115150.016.5546072
Produced by FastQC (version 0.11.5)