##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138816_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18015164
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13635568346755	33.0	31.0	34.0	30.0	34.0
2	32.346249914794	34.0	31.0	34.0	30.0	34.0
3	32.420581516771094	34.0	31.0	34.0	30.0	34.0
4	35.97702740868748	37.0	35.0	37.0	35.0	37.0
5	35.736383859730616	37.0	35.0	37.0	35.0	37.0
6	35.79155499222766	37.0	35.0	37.0	35.0	37.0
7	35.791579416096354	37.0	35.0	37.0	35.0	37.0
8	35.76348763741479	37.0	35.0	37.0	35.0	37.0
9	37.580407982963685	39.0	37.0	39.0	35.0	39.0
10-11	37.422677584283996	39.0	37.0	39.0	34.5	39.0
12-13	37.42989414362256	39.0	37.0	39.0	35.0	39.0
14-15	38.63878383233147	40.0	38.0	41.0	34.5	41.0
16-17	38.5913644749501	40.0	38.0	41.0	34.5	41.0
18-19	38.67068795488068	40.0	38.0	41.0	35.0	41.0
20-21	38.69996424147058	40.0	38.0	41.0	35.0	41.0
22-23	38.65462661076069	40.0	38.0	41.0	35.0	41.0
24-25	38.614968308403334	40.0	38.0	41.0	34.0	41.0
26-27	38.50273405230427	40.0	38.0	41.0	34.0	41.0
28-29	38.4287068505097	40.0	38.0	41.0	34.0	41.0
30-31	38.27918093881473	40.0	38.0	41.0	34.0	41.0
32-33	38.13336599017667	40.0	38.0	41.0	34.0	41.0
34-35	38.083368114507905	40.0	38.0	41.0	34.0	41.0
36-37	38.0280999095981	40.0	37.0	41.0	33.5	41.0
38-39	37.9565955377421	40.0	37.0	41.0	33.0	41.0
40-41	37.871110069183835	40.0	37.0	41.0	33.0	41.0
42-43	37.890622326333514	40.0	37.0	41.0	33.0	41.0
44-45	37.82758650740216	40.0	37.0	41.0	33.0	41.0
46-47	37.77581403851027	40.0	37.0	41.0	33.0	41.0
48-49	37.73789793533139	40.0	37.0	41.0	33.0	41.0
50-51	37.684181355167915	39.5	37.0	41.0	33.0	41.0
52-53	37.430499104025216	39.0	36.0	40.5	32.0	41.0
54-55	37.2818585779651	39.0	36.0	40.0	32.0	41.0
56-57	37.1902952392292	39.0	36.0	40.0	31.5	41.0
58-59	37.06414454828641	39.0	35.5	40.0	31.0	41.0
60-61	36.898935795280096	39.0	35.0	40.0	31.0	41.0
62-63	36.66560450760349	38.0	35.0	40.0	31.0	41.0
64-65	36.44853932144419	38.0	35.0	40.0	31.0	41.0
66-67	36.206323981529096	38.0	34.5	40.0	30.5	41.0
68-69	35.992689725988896	37.0	34.0	40.0	30.0	41.0
70-71	35.70528354180184	37.0	34.0	39.0	30.0	41.0
72-73	35.43983526999662	36.0	34.0	39.0	30.0	40.5
74-75	35.01041840547445	36.0	34.0	39.0	29.0	40.0
76-77	33.046889856704844	34.5	31.0	36.5	27.0	39.0
78-79	34.30460152688794	35.0	33.0	37.0	29.0	39.0
80-81	34.31801516629184	35.0	34.0	37.0	29.0	39.0
82-83	34.04916235374188	35.0	33.0	36.5	29.0	39.0
84-85	33.75413011553823	35.0	33.0	36.0	29.0	37.5
86-87	33.50403753866511	35.0	33.0	36.0	29.0	37.0
88-89	33.349846362695274	35.0	33.0	35.0	29.0	37.0
90-91	33.16633227782094	35.0	33.0	35.0	29.0	36.0
92-93	33.122588642500574	34.5	33.0	35.0	29.0	36.0
94-95	32.987220854226834	34.0	32.5	35.0	29.0	36.0
96-97	33.030558765699865	34.0	33.0	35.0	29.0	35.5
98-99	33.16465088585474	34.0	33.0	35.0	29.0	35.0
100-101	31.909322847682773	33.0	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	7.0
19	31.0
20	139.0
21	620.0
22	2334.0
23	7398.0
24	18675.0
25	39051.0
26	71603.0
27	115348.0
28	170892.0
29	239298.0
30	320493.0
31	422148.0
32	557180.0
33	763347.0
34	1104461.0
35	1683303.0
36	2773439.0
37	4263337.0
38	4098983.0
39	1348615.0
40	14462.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.57628540045486	21.012731274608434	13.843576444821707	23.567406880114998
2	14.526783912222813	21.98996417887515	41.81775500375575	21.66549690514629
3	18.198486197956637	27.202502594379485	30.621871167236243	23.977140040427635
4	12.08126028061239	18.413315408369392	39.710717104579885	29.794707206438332
5	12.450688610914794	38.71809705498296	36.126200298105346	12.705014035996898
6	28.883028492561348	39.047908064617836	19.248343388608482	12.820720054212337
7	25.638034713422535	33.02612732251563	24.211425441367062	17.124412522694772
8	23.655815733900617	37.85989958237405	21.868116215872362	16.616168467852972
9	25.636130761840413	16.322465895952988	21.874505277887007	36.16689806431959
10-11	23.721008693593042	27.314453737819257	29.56042003171384	19.404117536873866
12-13	24.407930858058887	25.061419653700785	29.77327670126432	20.75737278697601
14-15	21.468451777967736	26.312479813751267	27.486195776825134	24.732872631455862
16-17	20.88494244482796	29.377206372259256	28.2236105846252	21.514240598287586
18-19	20.903881307991423	28.607646869048764	29.953185549684697	20.535286273275112
20-21	21.823223365656663	27.812665587183826	29.78111426465267	20.58299678250684
22-23	21.497652494454858	27.80791661238827	29.620961843142645	21.07346905001423
24-25	21.138639594868092	27.90189905512699	29.795751298964746	21.163710051040173
26-27	21.143970976122493	28.10166278164638	29.667695021986844	21.08667122024428
28-29	21.07416162002068	28.24580889568788	29.644750753039823	21.035278731251616
30-31	21.312523880066298	28.084511231028657	29.56843935836882	21.03452553053623
32-33	21.108473991987093	28.19603971824769	29.538819645053355	21.156666644711862
34-35	21.232335794108714	28.25189138632303	29.52738391307327	20.988388906494986
36-37	21.214695881366747	28.211352865669642	29.459047178791025	21.114904074172586
38-39	21.101497033111382	28.311010893690263	29.51154765140191	21.07594442179644
40-41	21.312457581034753	28.205063991937134	29.361886130989625	21.12059229603849
42-43	21.206764303414012	28.322039487565178	29.217539660209585	21.253656548811225
44-45	21.344773852686192	28.490440149203277	28.94152151089729	21.223264487213246
46-47	21.36629318964317	28.466181240074224	28.73568240254173	21.431843167740883
48-49	21.364632865124655	28.527544496781914	28.67640708298277	21.431415555110668
50-51	21.331608783606608	28.63074884649369	28.568476320881857	21.46916604901784
52-53	21.478294007201086	28.588763768442927	28.411933374021615	21.521008850334375
54-55	21.485603550251533	28.55336824005551	28.4490141001874	21.512014109505554
56-57	21.50491528132992	28.607755016722408	28.38794142238835	21.499388279559316
58-59	21.437047438146838	28.64116992864194	28.468380146709876	21.453402486501343
60-61	21.54239031837612	28.59029906877499	28.40359866840788	21.46371194444101
62-63	21.481567011876844	28.617494608813278	28.380499386636192	21.52043899267369
64-65	21.45608700242644	28.627809921660063	28.40852103992717	21.507582035986328
66-67	21.534857253279657	28.61445411323635	28.268410172923414	21.58227846056058
68-69	21.518196239937026	28.613471673108986	28.340317076684467	21.52801501026952
70-71	21.485634908815385	28.434918534427972	28.403496937757964	21.675949618998676
72-73	21.521062993126694	28.50038515019252	28.39862269006874	21.57992916661204
74-75	21.48316315417055	28.524819279064513	28.365410763837684	21.626606802927252
76-77	21.46946306661768	28.52548550270186	28.345738048234608	21.659313382445852
78-79	21.52646182202952	28.47242702901338	28.309378900271298	21.6917322486858
80-81	21.44617334094425	28.501236306312244	28.36183803615933	21.690752316584174
82-83	21.525150162526845	28.45385891828407	28.45141447813477	21.569576441054316
84-85	21.720402384850438	28.37306668520789	28.348447021405754	21.558083908535917
86-87	21.535329968669913	28.439672252888908	28.404933162347145	21.620064616094034
88-89	21.564720318162188	28.404586018358497	28.40055073645744	21.630142927021872
90-91	21.6368772199645	28.324090829534278	28.442406253320236	21.59662569718099
92-93	21.601713851645197	28.310623753567654	28.474630727086492	21.613031667700657
94-95	21.45977215811445	28.346912877317624	28.55448847828307	21.638826486284852
96-97	21.456634927163826	28.40527216892595	28.555301440449334	21.582791463460893
98-99	21.68630717330507	28.997784151890905	28.58385660022515	20.732052074578874
100-101	22.371832220460163	31.568291379698326	25.865698530733503	20.194177869108007
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4311.0
1	3846.0
2	5676.0
3	8497.5
4	11505.0
5	19052.5
6	25865.5
7	31478.5
8	35400.5
9	34264.0
10	29202.0
11	24130.0
12	25376.5
13	34029.0
14	54460.5
15	81773.5
16	102350.0
17	112389.0
18	114879.0
19	112456.0
20	108006.5
21	104937.0
22	108089.5
23	122567.5
24	147625.5
25	180338.5
26	220031.0
27	261305.0
28	300473.5
29	339407.5
30	376250.5
31	412299.0
32	451856.0
33	490527.0
34	528998.0
35	565997.5
36	601490.0
37	632553.0
38	654352.5
39	671244.5
40	688626.5
41	705060.0
42	714475.0
43	722511.5
44	729297.0
45	734661.0
46	732593.0
47	723501.5
48	711577.0
49	694407.5
50	676960.5
51	651811.0
52	615043.5
53	579192.5
54	543948.5
55	500944.0
56	455013.0
57	406656.0
58	355455.5
59	307445.0
60	260361.5
61	212702.0
62	168306.5
63	130095.0
64	97408.0
65	71677.5
66	53279.5
67	40228.0
68	30353.5
69	21741.5
70	15379.0
71	11086.5
72	8021.5
73	5702.5
74	3988.0
75	2786.0
76	1979.5
77	1359.0
78	922.5
79	652.0
80	480.5
81	349.0
82	266.0
83	195.5
84	134.5
85	98.5
86	84.5
87	74.0
88	54.5
89	41.5
90	28.0
91	16.5
92	13.0
93	8.5
94	3.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	5.328844078244307E-4
3	0.0014376777252763282
4	3.6080715113112486E-4
5	3.1084923789758453E-4
6	4.218668228610076E-4
7	0.0
8	0.0
9	0.0
10-11	2.775439624085576E-6
12-13	9.1589507594824E-4
14-15	6.41126553163768E-4
16-17	8.326318872256728E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.1660412975785524E-5
28-29	1.4452518183143923E-4
30-31	4.453806097427565E-5
32-33	9.684206942292593E-4
34-35	0.001691052776945239
36-37	0.0012009410652937876
38-39	6.737736004066195E-4
40-41	3.137522816208534E-5
42-43	5.46160838560404E-5
44-45	5.802290210364352E-4
46-47	2.932228086645815E-5
48-49	0.0
50-51	0.0
52-53	8.422149020297289E-4
54-55	0.0032734257844186333
56-57	0.005950855767485803
58-59	0.004296585872018491
60-61	2.0699840272505243E-4
62-63	4.434907422416284E-5
64-65	1.0554871514589518E-4
66-67	1.5510830793057792E-4
68-69	3.267477541319868E-6
70-71	1.1570274708073705E-4
72-73	1.3377913893592341E-5
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	3.2817435947475474E-5
88-89	2.0342809630508003E-4
90-91	1.014743242148255E-4
92-93	4.398591150378843E-4
94-95	2.4563715275191983E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	418.0
20-21	1353.0
22-23	2757.0
24-25	5949.0
26-27	10391.0
28-29	21419.0
30-31	44730.0
32-33	53512.0
34-35	72062.0
36-37	107947.0
38-39	132606.0
40-41	132476.0
42-43	151584.0
44-45	180226.0
46-47	179617.0
48-49	167813.0
50-51	151765.0
52-53	141381.0
54-55	151265.0
56-57	159620.0
58-59	162855.0
60-61	161404.0
62-63	151321.0
64-65	156035.0
66-67	169160.0
68-69	175796.0
70-71	177216.0
72-73	168710.0
74-75	169201.0
76-77	165399.0
78-79	181951.0
80-81	188966.0
82-83	180481.0
84-85	179429.0
86-87	189502.0
88-89	208959.0
90-91	229599.0
92-93	239251.0
94-95	277068.0
96-97	403270.0
98-99	1512978.0
100-101	1.0697722E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.60139973451494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.90019962787149	71.72779326577603
2	7.862457182787061	12.831750229362305
3	2.2012524929411015	5.388758537792531
4	0.9008153352790569	2.940311690443497
5	0.44857268728480165	1.8302079582556336
6	0.2320103977116757	1.1359423923740544
7	0.13367036726976278	0.7635382350567536
8	0.08315667728545784	0.5428561011011763
9	0.05892587683430118	0.43275906272363435
>10	0.1752954653723765	2.1004244729262203
>50	0.0027807689713137966	0.15144291340777344
>100	8.13317722985901E-4	0.10228370195452803
>500	2.493134825924854E-5	0.014141505492329697
>1k	2.4871320324541926E-5	0.03778993333357049
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1101758496342304E-5	0.0	5.550879248171152E-6	0.0
3	0.0	1.1101758496342304E-5	0.0	1.1101758496342304E-5	0.0
4	0.0	2.775439624085576E-5	0.0	1.6652637744513455E-5	0.0
5	0.0	3.330527548902691E-5	0.0	2.220351699268461E-5	0.0
6	0.0	3.330527548902691E-5	0.0	2.220351699268461E-5	0.0
7	0.0	3.330527548902691E-5	0.0	2.220351699268461E-5	0.0
8	0.0	3.330527548902691E-5	0.0	2.220351699268461E-5	0.0
9	0.0	8.881406797073843E-5	0.0	3.885615473719806E-5	5.550879248171152E-6
10-11	0.0	9.436494721890958E-5	0.0	3.885615473719806E-5	5.550879248171152E-6
12-13	0.0	9.436494721890958E-5	0.0	5.828423210579709E-5	5.550879248171152E-6
14-15	0.0	1.3044566233202205E-4	0.0	7.216143022622497E-5	1.387719812042788E-5
16-17	0.0	1.387719812042788E-4	0.0	1.0824214533933745E-4	3.0529835864941334E-5
18-19	5.550879248171152E-6	1.5542461894879227E-4	0.0	1.6097549819696342E-4	3.330527548902691E-5
20-21	5.550879248171152E-6	1.6652637744513457E-4	0.0	1.8872989443781917E-4	3.330527548902691E-5
22-23	5.550879248171152E-6	1.8317901518964802E-4	0.0	2.6921764353630083E-4	3.6080715113112484E-5
24-25	5.550879248171152E-6	2.1370885105458933E-4	0.0	3.524808322588681E-4	3.885615473719806E-5
26-27	5.550879248171152E-6	2.2203516992684607E-4	0.0	5.911686399302277E-4	3.885615473719806E-5
28-29	5.550879248171152E-6	2.2758604917501722E-4	0.0	0.002511772859797446	4.163159436128364E-5
30-31	5.550879248171152E-6	2.9142116052898544E-4	0.0	0.006847009552619116	4.440703398536922E-5
32-33	5.550879248171152E-6	2.9419660015307105E-4	0.0	0.014579384345321529	4.440703398536922E-5
34-35	5.550879248171152E-6	3.441545133866114E-4	0.0	0.02274750315900538	4.440703398536922E-5
36-37	5.550879248171152E-6	3.913369869960662E-4	0.0	0.03412958105738033	4.440703398536922E-5
38-39	5.550879248171152E-6	3.9966330586832294E-4	0.0	0.051795254264685016	4.995791323354037E-5
40-41	5.550879248171152E-6	4.2186682286100754E-4	0.0	0.06848952360355975	4.995791323354037E-5
42-43	5.550879248171152E-6	4.38519460605521E-4	0.0	0.0796412400131356	4.995791323354037E-5
44-45	5.550879248171152E-6	4.7182473609454786E-4	0.0	0.09143130753625112	5.550879248171152E-5
46-47	5.550879248171152E-6	4.884773738390613E-4	0.0	0.10373760682944658	6.105967172988267E-5
48-49	5.550879248171152E-6	4.995791323354037E-4	0.0	0.11449798625202634	6.105967172988267E-5
50-51	5.550879248171152E-6	5.412107266966873E-4	0.0	0.12472270582715761	6.105967172988267E-5
52-53	5.550879248171152E-6	5.49537045568944E-4	0.0	0.13373733372618757	7.493686985031054E-5
54-55	5.550879248171152E-6	5.523124851930295E-4	0.0	0.14248829486092937	8.881406797073843E-5
56-57	5.550879248171152E-6	5.661896833134575E-4	0.0	0.15082016461243428	8.881406797073843E-5
58-59	5.550879248171152E-6	6.328002342915113E-4	0.0	0.1588800412807788	8.881406797073843E-5
60-61	5.550879248171152E-6	6.439019927878537E-4	0.0	0.16686220563964893	9.158950759482401E-5
62-63	5.550879248171152E-6	6.494528720360248E-4	0.0	0.17463343658708852	9.436494721890958E-5
64-65	5.550879248171152E-6	6.661055097805383E-4	0.0	0.18161921812091192	9.436494721890958E-5
66-67	5.550879248171152E-6	6.716563890287094E-4	0.0	0.18888531905676795	9.991582646708073E-5
68-69	5.550879248171152E-6	6.93859906021394E-4	0.0	0.19617917438886484	1.0546670571525188E-4
70-71	5.550879248171152E-6	6.93859906021394E-4	0.0	0.20311777344907878	1.0546670571525188E-4
72-73	5.550879248171152E-6	6.966353456454796E-4	0.0	0.2080802594969438	1.387719812042788E-4
74-75	5.550879248171152E-6	7.049616645177362E-4	0.0	0.21203803640088983	1.4432286045244994E-4
76-77	5.550879248171152E-6	7.243897418863353E-4	0.0	0.21552953944798947	1.498737397006211E-4
78-79	5.550879248171152E-6	7.327160607585921E-4	0.0	0.21858529847410768	1.498737397006211E-4
80-81	5.550879248171152E-6	7.382669400067632E-4	0.0	0.22011179026735478	1.5820005857287783E-4
82-83	5.550879248171152E-6	7.438178192549344E-4	0.0	0.22077512033751123	1.6652637744513457E-4
84-85	5.550879248171152E-6	7.715722154957901E-4	0.0	0.22135796265856922	1.6652637744513457E-4
86-87	5.550879248171152E-6	8.104283702329881E-4	5.550879248171152E-6	0.22156334519075152	1.7207725669330572E-4
88-89	5.550879248171152E-6	8.38182766473844E-4	5.550879248171152E-6	0.22162995574172958	1.7207725669330572E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	23040	0.0	48.910084	1
GTATCAA	41165	0.0	36.28815	1
ATCAACG	49975	0.0	28.928932	3
TCAACGC	50275	0.0	28.772686	4
CAACGCA	50735	0.0	28.445154	5
AACGCAG	52540	0.0	27.524117	6
TATCAAC	53595	0.0	27.34481	2
ACGCAGA	60770	0.0	23.76193	7
TACATGG	64020	0.0	23.623217	2
CGCAGAG	61555	0.0	23.438343	8
GTACATG	65685	0.0	23.435328	1
ACATGGG	64950	0.0	22.681086	3
CATGGGG	41005	0.0	20.148188	4
GCAGAGT	73135	0.0	19.583342	9
AGAGTAC	67035	0.0	18.24175	10-11
GAGTACT	42525	0.0	18.134035	12-13
CAGAGTA	71540	0.0	17.756239	10-11
GTGGTAT	12185	0.0	17.09802	1
ATGGGGG	25695	0.0	16.642757	5
TGGTATC	11515	0.0	16.554607	2
>>END_MODULE