FastQCFastQC Report
Fri 27 May 2016
SRR2138815_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138815_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11686154
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108500.044.6483271
GTATCAA181350.030.754421
TCAACGC202400.026.3690134
ATCAACG204800.026.1015853
CAACGCA208850.025.5947695
AACGCAG216100.024.8335466
TATCAAC227000.023.8830492
GTACATG281250.023.5299361
TACATGG278050.022.9220332
ACATGGG280700.021.9101663
ACGCAGA261350.020.4371727
CGCAGAG265000.020.1238888
CATGGGG211800.019.5304994
GAGTACA193950.017.0279881
AGAGTAC283250.016.65648510-11
GAGTACT197950.016.33266812-13
GCAGAGT336700.015.9390169
CAGAGTA318050.015.76110810-11
ATGGGGG142750.015.314945
AGTACAT207800.015.2038232
Produced by FastQC (version 0.11.5)