##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138815_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11686154
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44649300360067	33.0	31.0	34.0	31.0	34.0
2	32.6302229116611	34.0	31.0	34.0	31.0	34.0
3	32.713604578546544	34.0	31.0	34.0	31.0	34.0
4	36.175604737024685	37.0	35.0	37.0	35.0	37.0
5	35.98735640485313	37.0	35.0	37.0	35.0	37.0
6	36.056276513213845	37.0	35.0	37.0	35.0	37.0
7	36.05504847873817	37.0	35.0	37.0	35.0	37.0
8	36.041310682710495	37.0	35.0	37.0	35.0	37.0
9	37.909631860062774	39.0	38.0	39.0	35.0	39.0
10-11	37.779592798451915	39.0	37.5	39.0	35.0	39.0
12-13	37.78048509372715	39.0	38.0	39.0	35.0	39.0
14-15	39.11469586144423	40.0	38.5	41.0	36.0	41.0
16-17	39.08733617578547	40.0	38.0	41.0	36.0	41.0
18-19	39.14766761588115	40.0	38.5	41.0	36.0	41.0
20-21	39.160275063537725	40.0	39.0	41.0	36.0	41.0
22-23	39.12075936788499	40.0	39.0	41.0	36.0	41.0
24-25	39.08949932495082	40.0	39.0	41.0	36.0	41.0
26-27	39.000580524681624	40.0	39.0	41.0	35.5	41.0
28-29	38.93775752153805	40.0	38.0	41.0	35.0	41.0
30-31	38.810886015523835	40.0	38.0	41.0	35.0	41.0
32-33	38.683914242886416	40.0	38.0	41.0	35.0	41.0
34-35	38.64381487527268	40.0	38.0	41.0	35.0	41.0
36-37	38.593786863651104	40.0	38.0	41.0	35.0	41.0
38-39	38.52583977665129	40.0	38.0	41.0	34.5	41.0
40-41	38.440532593949236	40.0	38.0	41.0	34.0	41.0
42-43	38.45405938879796	40.0	38.0	41.0	34.0	41.0
44-45	38.39493214696607	40.0	38.0	41.0	34.0	41.0
46-47	38.34081984469959	40.0	38.0	41.0	34.0	41.0
48-49	38.29328153337494	40.0	38.0	41.0	34.0	41.0
50-51	38.22716585276943	40.0	38.0	41.0	34.0	41.0
52-53	37.96738226598702	40.0	37.0	41.0	33.0	41.0
54-55	37.81532253075842	40.0	37.0	41.0	33.0	41.0
56-57	37.719246921302	39.0	36.5	41.0	33.0	41.0
58-59	37.58526941220623	39.0	36.0	41.0	33.0	41.0
60-61	37.39420812517392	39.0	36.0	40.5	32.5	41.0
62-63	37.1343431364926	39.0	35.0	40.0	32.0	41.0
64-65	36.89302475224049	38.5	35.0	40.0	32.0	41.0
66-67	36.62297665085068	38.0	35.0	40.0	31.0	41.0
68-69	36.383212226422316	37.0	35.0	40.0	31.0	41.0
70-71	36.07327733999081	37.0	35.0	39.0	31.0	41.0
72-73	35.781710405872666	36.0	34.5	39.0	31.0	41.0
74-75	35.33929792916069	36.0	34.0	39.0	30.5	40.0
76-77	33.42649620982445	34.5	32.0	36.5	27.5	39.0
78-79	34.611154339022214	35.0	34.0	37.0	30.0	39.0
80-81	34.589770375453796	35.0	34.0	37.0	30.0	39.0
82-83	34.32787196647748	35.0	34.0	36.0	30.0	38.5
84-85	34.051374058085216	35.0	34.0	36.0	30.0	37.0
86-87	33.82718288856408	35.0	34.0	35.5	30.0	37.0
88-89	33.69586378657543	35.0	34.0	35.0	30.0	36.5
90-91	33.53699376755468	35.0	33.5	35.0	30.0	36.0
92-93	33.50687505738004	35.0	33.0	35.0	30.0	36.0
94-95	33.38097333561488	35.0	33.0	35.0	30.0	36.0
96-97	33.4069910457829	35.0	33.0	35.0	30.0	35.5
98-99	33.50992552061368	35.0	33.0	35.0	30.0	35.0
100-101	32.28988898344795	34.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	11.0
20	54.0
21	208.0
22	951.0
23	3261.0
24	8758.0
25	19002.0
26	35241.0
27	57788.0
28	85457.0
29	119062.0
30	157841.0
31	207133.0
32	274015.0
33	372838.0
34	548309.0
35	859872.0
36	1534919.0
37	2816190.0
38	3251498.0
39	1313175.0
40	20567.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58895612705429	19.76118062452369	12.769701648634785	24.880161599787236
2	15.171276626969435	21.162118518022837	40.30178922075978	23.364815634247947
3	18.63001097052335	25.472340075955042	30.19621148978338	25.701437463738223
4	12.603878744501696	17.125686317446682	38.429720913759	31.84071402429262
5	13.163901418008217	37.811898056752845	35.91856486033419	13.105635664904746
6	31.089509556358998	37.62166705330858	18.171607910359775	13.117215479972646
7	26.833242142795655	32.197111213834766	23.282407539726073	17.68723910364351
8	24.601079191665622	37.05317420941056	21.253741821304082	17.09200477761974
9	25.802749133718418	15.802273357000088	21.012918364758843	37.38205914452266
10-11	24.499956294429985	26.471148098473975	29.070532061725874	19.958363545370165
12-13	24.880350777658975	24.017966744066744	29.251525431850922	21.85015704642336
14-15	21.935223593956234	25.439997508142287	26.472685127721185	26.15209377018029
16-17	21.91511242507042	28.51922264487869	27.29270548638862	22.272959443662273
18-19	22.02133824353162	27.932932425843436	28.60390595571477	21.44182337491017
20-21	22.595302525576564	27.2897818475027	28.44786779793077	21.667047828989972
22-23	22.324362611747834	27.224995997983527	28.321768885720083	22.128872504548564
24-25	22.10954586555342	27.304484627039482	28.44546274071255	22.140506766694546
26-27	22.125158805163522	27.462078350188495	28.32710785410501	22.085654990542974
28-29	22.046298894799193	27.57574770829897	28.34766588187912	22.03028751502272
30-31	22.297349900983622	27.445579189797026	28.25359419877592	22.00347671044343
32-33	22.083375705991607	27.555768479209398	28.249433461599654	22.11142235319934
34-35	22.191024190354362	27.584687658205603	28.26126138886015	21.963026762579887
36-37	22.220523506852697	27.541234194009196	28.193203853033626	22.045038446104478
38-39	22.06856425255468	27.640287200014896	28.268704413658654	22.02244413377177
40-41	22.256611388088736	27.52938358007364	28.156570916718376	22.05743411511925
42-43	22.141188192856372	27.647489988915407	28.03448983690448	22.176831981323737
44-45	22.19865226896836	27.684470696150964	27.929160157451726	22.187716877428947
46-47	22.255910130936893	27.67172719752412	27.763196256577743	22.309166414961236
48-49	22.19094298157405	27.79251973686616	27.750902282857904	22.265634998701884
50-51	22.15298788612727	27.886043262538756	27.697155766593728	22.263813084740242
52-53	22.344208829496758	27.796352566599587	27.56577058718439	22.293668016719266
54-55	22.290098689963347	27.691184353310007	27.615748135949413	22.402968820777236
56-57	22.212377064280435	27.77656728594586	27.664654615858254	22.346401033915452
58-59	22.246387008990204	27.815928518112393	27.606001155957937	22.331683316939465
60-61	22.298181232131153	27.7371205374169	27.658695807478946	22.306002422973002
62-63	22.249388597748062	27.82969754357776	27.625062061280314	22.29585179739387
64-65	22.2261375207344	27.824681036267286	27.59919142314412	22.3499900198542
66-67	22.264022641752597	27.85828136585078	27.478287073570634	22.399408918825987
68-69	22.221650483864693	27.8982052140823	27.493126906190398	22.387017395862607
70-71	22.203909149827396	27.740668381596535	27.543450766144034	22.511971702432035
72-73	22.207540570171304	27.770627171702873	27.49693767397702	22.524894584148804
74-75	22.172509349385543	27.822942075065388	27.482467356267627	22.522081219281446
76-77	22.268362523459416	27.77030820906599	27.451603480524394	22.5097257869502
78-79	22.23753594693348	27.771549321517497	27.387322630174527	22.603592101374495
80-81	22.229497057020243	27.83068088394763	27.39037719032254	22.549444868709585
82-83	22.250777392023526	27.80999644357675	27.452660322989885	22.486565841409835
84-85	22.314802800519328	27.802986215732606	27.39671289615464	22.485498087593427
86-87	22.2348536365772	27.838336504924865	27.463348728572086	22.46346112992585
88-89	22.22762697726355	27.83430870521469	27.448652627608155	22.489411689913602
90-91	22.208839833619045	27.81028570966113	27.54599076066017	22.43488369605965
92-93	22.20823785794719	27.75545296744928	27.56123837215345	22.47507080245008
94-95	22.105142715046878	27.83478342865705	27.64663422477374	22.41343963152233
96-97	22.09449176894915	27.873926056756492	27.63634058772586	22.395241586568496
98-99	22.366075393199623	28.450437086948178	27.743141514484197	21.440346005368003
100-101	26.528699791158637	35.38393696938067	28.32614761591356	9.761215623547132
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3832.0
1	3067.0
2	3372.0
3	4733.5
4	6043.0
5	9279.5
6	12431.5
7	15019.5
8	16793.0
9	16530.0
10	14449.5
11	12197.5
12	12595.5
13	16056.0
14	24230.0
15	34922.0
16	42794.5
17	47042.0
18	48999.0
19	49551.0
20	49472.5
21	50210.0
22	53518.0
23	61249.5
24	73864.0
25	90512.0
26	110788.0
27	130025.5
28	148234.0
29	168590.0
30	188565.5
31	209697.5
32	233977.0
33	258749.5
34	282804.5
35	307588.5
36	334890.5
37	358799.5
38	379622.0
39	399456.5
40	417870.5
41	437213.0
42	452804.0
43	465585.0
44	478407.5
45	490523.0
46	498948.5
47	503508.0
48	504478.5
49	500399.5
50	493385.5
51	481103.5
52	461969.5
53	440416.5
54	417430.5
55	389701.0
56	360297.0
57	329026.0
58	294105.5
59	256811.5
60	218003.0
61	180935.0
62	146864.0
63	116812.0
64	89856.0
65	67712.0
66	51433.5
67	39651.5
68	30569.5
69	22846.5
70	17003.0
71	12655.0
72	9190.5
73	6587.0
74	4693.5
75	3308.0
76	2340.0
77	1576.0
78	1097.0
79	799.0
80	554.5
81	380.5
82	278.0
83	193.0
84	137.5
85	101.0
86	80.5
87	66.5
88	49.0
89	32.5
90	19.5
91	13.5
92	11.5
93	6.5
94	2.5
95	1.5
96	1.0
97	1.0
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0010011848209428014
3	0.002515797755189603
4	4.535281667518672E-4
5	3.765139497562671E-4
6	5.219852485257339E-4
7	0.0
8	0.0
9	0.0
10-11	4.2785676108666714E-6
12-13	0.001018299091386268
14-15	8.000921432320677E-4
16-17	1.2835702832600016E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.4250613278637386E-5
28-29	2.056581880365376E-4
30-31	2.5750860765439187E-5
32-33	0.001295370792358087
34-35	0.0019733478857304624
36-37	0.0013843157288296465
38-39	7.167973146324993E-4
40-41	3.08269320108051E-5
42-43	2.2191371284720618E-5
44-45	6.582686218707556E-4
46-47	9.048726396284086E-6
48-49	9.141669457161269E-6
50-51	0.0
52-53	0.0010106197506861618
54-55	0.003767206374564123
56-57	0.00691315592063678
58-59	0.00481665839870814
60-61	2.273315608565621E-4
62-63	2.443116431502051E-5
64-65	8.38874345110537E-5
66-67	1.945129687033122E-4
68-69	0.0
70-71	1.429333058901386E-4
72-73	2.065736592865989E-5
74-75	0.0
76-77	0.0
78-79	5.344275273785886E-6
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	2.905229367288895E-4
90-91	1.2139517041454021E-4
92-93	7.105003496131473E-4
94-95	4.3594337609121703E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	257.0
20-21	828.0
22-23	1679.0
24-25	3586.0
26-27	6763.0
28-29	15589.0
30-31	30864.0
32-33	36402.0
34-35	50204.0
36-37	77220.0
38-39	87584.0
40-41	86922.0
42-43	95276.0
44-45	112838.0
46-47	113748.0
48-49	108707.0
50-51	99493.0
52-53	89685.0
54-55	97930.0
56-57	101945.0
58-59	103872.0
60-61	107513.0
62-63	99017.0
64-65	105241.0
66-67	113127.0
68-69	116007.0
70-71	114969.0
72-73	107798.0
74-75	109119.0
76-77	107407.0
78-79	115983.0
80-81	118579.0
82-83	112995.0
84-85	111519.0
86-87	117395.0
88-89	125745.0
90-91	133515.0
92-93	137967.0
94-95	160584.0
96-97	240400.0
98-99	981728.0
100-101	7028154.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.4013038963185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.85629753842248	76.73844973076834
2	7.164553555485623	12.237244309469535
3	1.6916185660297152	4.333992937024746
4	0.6074637844128317	2.0751279703459176
5	0.27342083413064866	1.1675247873588208
6	0.13642149868832903	0.6990344320483921
7	0.07699646494399055	0.46029189511367796
8	0.04965849701109352	0.3392720315423057
9	0.031181690235665686	0.23966613034352566
>10	0.10940147529535979	1.455032974100808
>50	0.0023574057445621584	0.1368345857257693
>100	5.960238360775685E-4	0.09070526404401284
>500	1.6332900760149306E-5	0.012595504722573078
>1k	1.6332862846969115E-5	0.014227447391607168
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.5671405665200032E-5	0.0	8.557135221733343E-6	0.0
3	0.0	2.5671405665200032E-5	0.0	8.557135221733343E-6	0.0
4	0.0	6.845708177386674E-5	0.0	8.557135221733343E-6	0.0
5	0.0	6.845708177386674E-5	0.0	8.557135221733343E-6	0.0
6	4.278567610866672E-5	6.845708177386674E-5	0.0	8.557135221733343E-6	0.0
7	4.278567610866672E-5	6.845708177386674E-5	0.0	3.422854088693337E-5	8.557135221733343E-6
8	4.278567610866672E-5	7.70142169956001E-5	0.0	3.422854088693337E-5	8.557135221733343E-6
9	4.278567610866672E-5	1.8825697487813355E-4	0.0	8.557135221733344E-5	2.5671405665200032E-5
10-11	4.706424371953339E-5	1.8825697487813355E-4	0.0	1.3263559593686684E-4	2.5671405665200032E-5
12-13	5.1342811330400064E-5	1.8825697487813355E-4	0.0	1.5830700160206684E-4	2.5671405665200032E-5
14-15	5.1342811330400064E-5	2.695497594846003E-4	0.0	1.8825697487813355E-4	3.850710849780005E-5
16-17	7.70142169956001E-5	2.7382832709546697E-4	0.0	2.267640833759336E-4	4.278567610866672E-5
18-19	7.70142169956001E-5	2.823854623172003E-4	0.0	2.4815692143026697E-4	4.278567610866672E-5
20-21	7.70142169956001E-5	2.909425975389337E-4	0.0	3.0377830037153373E-4	4.278567610866672E-5
22-23	7.70142169956001E-5	3.2944970603673374E-4	0.0	3.5512111170193374E-4	4.706424371953339E-5
24-25	8.98499198282001E-5	3.4656397648020045E-4	0.0	3.9790678781060046E-4	5.9899946552133403E-5
26-27	9.840705504993345E-5	3.5939967931280045E-4	0.0	5.562137894126674E-4	6.845708177386674E-5
28-29	1.0268562266080013E-4	3.5939967931280045E-4	0.0	0.001916798289668269	6.845708177386674E-5
30-31	1.1552132549340013E-4	4.7919957241706723E-4	0.0	0.00556641646173754	8.129278460646676E-5
32-33	1.1979989310426681E-4	4.7919957241706723E-4	0.0	0.011436611223846614	1.0268562266080013E-4
34-35	1.2835702832600013E-4	5.776066274670007E-4	0.0	0.01815396237290729	1.0268562266080013E-4
36-37	1.4547129876946684E-4	6.845708177386675E-4	0.0	0.027956160769402834	1.0268562266080013E-4
38-39	1.4547129876946684E-4	7.273564938473342E-4	0.0	0.046961558096872594	1.0268562266080013E-4
40-41	1.540284339912002E-4	7.958135756212009E-4	0.0	0.08876316365503997	1.0268562266080013E-4
42-43	1.540284339912002E-4	8.257635488972677E-4	0.0	0.10450829246302933	1.069641902716668E-4
44-45	1.540284339912002E-4	9.156134687254677E-4	0.0	0.12560163078460201	1.1552132549340013E-4
46-47	1.540284339912002E-4	9.926276857210679E-4	0.0	0.14951454516173585	1.1979989310426681E-4
48-49	1.5830700160206684E-4	0.0010054633885536678	0.0	0.17168180395363608	1.1979989310426681E-4
50-51	1.668641368238002E-4	0.0011081490112144678	0.0	0.19387901271881236	1.2407846071513348E-4
52-53	1.7114270443466687E-4	0.001120984714047068	0.0	0.22211755895053242	1.3691416354773349E-4
54-55	1.7114270443466687E-4	0.0011466561197122681	0.0	0.28072965665179495	1.4547129876946684E-4
56-57	1.7114270443466687E-4	0.0011637703901557347	0.0	0.344715635272306	1.4974986638033352E-4
58-59	1.7114270443466687E-4	0.0011808846605992013	0.0	0.40980120576880985	1.540284339912002E-4
60-61	1.8825697487813355E-4	0.0011894417958209348	0.0	0.4528863816102372	1.6258556921293352E-4
62-63	2.310426509868003E-4	0.0012022774986535348	0.0	0.4724864998356174	1.6258556921293352E-4
64-65	2.310426509868003E-4	0.0012921274184817348	0.0	0.4901869340417728	1.6258556921293352E-4
66-67	2.3959978620853361E-4	0.0013263559593686683	0.0	0.4983247696376413	1.6258556921293352E-4
68-69	2.3959978620853361E-4	0.001394813041142535	0.0	0.5052175420587475	1.668641368238002E-4
70-71	2.4815692143026697E-4	0.001420484446807735	0.0	0.5119434503430299	1.7969983965640022E-4
72-73	2.695497594846003E-4	0.0014290415820294683	0.0	0.5174414097229936	1.968141100998669E-4
74-75	2.7382832709546697E-4	0.0014461558524729349	0.0	0.5217542058747471	1.968141100998669E-4
76-77	2.7382832709546697E-4	0.0014846629609707352	0.0	0.5253182526945992	1.968141100998669E-4
78-79	2.823854623172003E-4	0.001497498663803335	0.0	0.5287624996213467	1.968141100998669E-4
80-81	2.823854623172003E-4	0.001497498663803335	0.0	0.5303156196640915	2.0537124532160026E-4
82-83	2.8666402992806703E-4	0.0015060557990250684	0.0	0.5308547191830606	2.310426509868003E-4
84-85	2.909425975389337E-4	0.0015060557990250684	0.0	0.5312569045384821	2.310426509868003E-4
86-87	2.909425975389337E-4	0.0015060557990250684	0.0	0.5314066544048623	2.310426509868003E-4
88-89	2.9949973276066703E-4	0.0015745128807989352	0.0	0.5314836686218579	2.310426509868003E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10850	0.0	44.648327	1
GTATCAA	18135	0.0	30.75442	1
TCAACGC	20240	0.0	26.369013	4
ATCAACG	20480	0.0	26.101585	3
CAACGCA	20885	0.0	25.594769	5
AACGCAG	21610	0.0	24.833546	6
TATCAAC	22700	0.0	23.883049	2
GTACATG	28125	0.0	23.529936	1
TACATGG	27805	0.0	22.922033	2
ACATGGG	28070	0.0	21.910166	3
ACGCAGA	26135	0.0	20.437172	7
CGCAGAG	26500	0.0	20.123888	8
CATGGGG	21180	0.0	19.530499	4
GAGTACA	19395	0.0	17.027988	1
AGAGTAC	28325	0.0	16.656485	10-11
GAGTACT	19795	0.0	16.332668	12-13
GCAGAGT	33670	0.0	15.939016	9
CAGAGTA	31805	0.0	15.761108	10-11
ATGGGGG	14275	0.0	15.31494	5
AGTACAT	20780	0.0	15.203823	2
>>END_MODULE