FastQCFastQC Report
Fri 27 May 2016
SRR2138814_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138814_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7759443
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79400.044.8695561
GTATCAA133250.032.3405571
TCAACGC151550.027.5260874
CAACGCA154200.027.1341975
ATCAACG153800.027.096973
AACGCAG159950.026.1850076
TATCAAC167150.025.2823522
ACGCAGA189050.022.0660067
GTACATG197450.021.9730641
CGCAGAG190350.021.8276448
TACATGG195050.021.5804062
ACATGGG197750.020.6949393
CATGGGG140400.018.6889084
GAGTACT142550.017.44132412-13
GCAGAGT235850.017.4222229
CAGAGTA229550.016.52831610-11
AGAGTAC209400.016.34575810-11
GTACTTT162750.015.30273614-15
GAGTACA138200.015.1533991
AGTACTT156800.014.35310312-13
Produced by FastQC (version 0.11.5)