##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138814_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7759443
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44252544931382	33.0	31.0	34.0	31.0	34.0
2	32.63404319098677	34.0	31.0	34.0	31.0	34.0
3	32.71762290154074	34.0	31.0	34.0	31.0	34.0
4	36.17466356799064	37.0	35.0	37.0	35.0	37.0
5	35.98857727287899	37.0	35.0	37.0	35.0	37.0
6	36.058433446833746	37.0	35.0	37.0	35.0	37.0
7	36.06120851715774	37.0	35.0	37.0	35.0	37.0
8	36.049251731084304	37.0	35.0	37.0	35.0	37.0
9	37.91607309957686	39.0	38.0	39.0	35.0	39.0
10-11	37.78956073264537	39.0	37.5	39.0	35.0	39.0
12-13	37.79333245955928	39.0	38.0	39.0	35.0	39.0
14-15	39.12628702859213	40.0	39.0	41.0	36.0	41.0
16-17	39.096387459769986	40.0	38.0	41.0	36.0	41.0
18-19	39.161439551782266	40.0	39.0	41.0	36.0	41.0
20-21	39.17998271456618	40.0	39.0	41.0	36.0	41.0
22-23	39.13875172198573	40.0	39.0	41.0	36.0	41.0
24-25	39.10858756836881	40.0	39.0	41.0	36.0	41.0
26-27	39.018725547865174	40.0	39.0	41.0	36.0	41.0
28-29	38.95653768048798	40.0	38.5	41.0	35.0	41.0
30-31	38.82852357618286	40.0	38.0	41.0	35.0	41.0
32-33	38.70171041551309	40.0	38.0	41.0	35.0	41.0
34-35	38.66414011045547	40.0	38.0	41.0	35.0	41.0
36-37	38.61863875258156	40.0	38.0	41.0	35.0	41.0
38-39	38.55307388842304	40.0	38.0	41.0	35.0	41.0
40-41	38.46677851452385	40.0	38.0	41.0	34.0	41.0
42-43	38.488910887535745	40.0	38.0	41.0	34.0	41.0
44-45	38.4333760924539	40.0	38.0	41.0	34.0	41.0
46-47	38.38740530562194	40.0	38.0	41.0	34.0	41.0
48-49	38.35230603773046	40.0	38.0	41.0	34.0	41.0
50-51	38.29518635652812	40.0	38.0	41.0	34.0	41.0
52-53	38.04824109593949	40.0	37.0	41.0	33.0	41.0
54-55	37.90186199414044	40.0	37.0	41.0	33.0	41.0
56-57	37.81094527909554	39.5	37.0	41.0	33.0	41.0
58-59	37.684080976193954	39.0	36.5	41.0	33.0	41.0
60-61	37.50878510079836	39.0	36.0	41.0	33.0	41.0
62-63	37.26585135919969	39.0	35.5	40.0	32.0	41.0
64-65	37.033160860274165	39.0	35.0	40.0	32.0	41.0
66-67	36.77622814342659	38.0	35.0	40.0	32.0	41.0
68-69	36.54324062954181	37.5	35.0	40.0	31.5	41.0
70-71	36.239441025899225	37.0	35.0	39.5	31.0	41.0
72-73	35.955337177217004	36.5	35.0	39.0	31.0	41.0
74-75	35.50419099974569	36.0	34.0	39.0	31.0	40.0
76-77	33.5717662551515	34.5	32.0	36.5	28.0	39.0
78-79	34.76571667831942	35.0	34.0	37.0	30.0	39.0
80-81	34.73803234916146	35.0	34.0	37.0	31.0	39.0
82-83	34.463025111677034	35.0	34.0	36.5	30.5	39.0
84-85	34.179618508648886	35.0	34.0	36.0	30.0	37.5
86-87	33.93982506783706	35.0	34.0	36.0	30.0	37.0
88-89	33.79330277897024	35.0	34.0	35.0	30.0	37.0
90-91	33.618682141132794	35.0	34.0	35.0	30.0	36.0
92-93	33.5756096251759	35.0	34.0	35.0	30.0	36.0
94-95	33.4410268681363	35.0	33.0	35.0	30.0	36.0
96-97	33.4619611927699	35.0	33.0	35.0	30.0	36.0
98-99	33.5547515155166	35.0	33.0	35.0	30.0	35.0
100-101	32.334675601355656	34.0	31.5	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	7.0
20	28.0
21	136.0
22	562.0
23	1962.0
24	5387.0
25	11837.0
26	21674.0
27	35466.0
28	53459.0
29	75201.0
30	101013.0
31	133116.0
32	176508.0
33	241513.0
34	354554.0
35	552157.0
36	987782.0
37	1823941.0
38	2213175.0
39	954298.0
40	15662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.98318100925543	20.23799904194154	13.227044776280977	24.551775172522046
2	14.730976468858259	21.39461711938987	41.27272073518273	22.601685676569137
3	18.393522590705963	26.076804856123797	30.325384781438984	25.20428777173125
4	12.320710962371049	17.70901557053782	39.08211693742221	30.888156529668926
5	12.932344237895316	38.045751656729706	36.06665865059638	12.955245454778602
6	30.13883911063891	38.257326939550715	18.718477802380153	12.885356147430224
7	26.298910888320204	32.436232858466774	23.723094557173756	17.541761696039266
8	24.014661877147624	37.592891654723154	21.526699790178238	16.865746677950984
9	25.524834707852094	16.264066892430296	21.459310932498635	36.751787467218975
10-11	24.108613473197334	26.834601841562716	29.35870070562415	19.698083979615806
12-13	24.583424432909567	24.38863404634902	29.523176924301747	21.50476459643967
14-15	21.706199513196363	25.835415057997785	26.87155300773263	25.586832421073225
16-17	21.53942126331226	28.89220485175758	27.650916100791097	21.917457784139067
18-19	21.548466816497008	28.3294625658053	29.033011776747376	21.08905884095031
20-21	22.23054354291076	27.639597457722687	28.871808057160642	21.258050942205912
22-23	21.9483452573022	27.591570957523214	28.715133950911465	21.744949834263117
24-25	21.69802819617716	27.63314442461005	28.86217484084394	21.806652538368848
26-27	21.744520642765437	27.83245820646718	28.69732852943674	21.725692621330637
28-29	21.697198605458762	27.934762324259488	28.733671996817968	21.634367073463785
30-31	21.928916395988463	27.78622506946445	28.65278532654367	21.632073208003423
32-33	21.681923469209433	27.928511414669995	28.61084775098958	21.778717365130994
34-35	21.805786319804945	28.003472192841052	28.627847241740362	21.56289424561364
36-37	21.807787060138704	27.958547466142363	28.55823277927151	21.675432694447426
38-39	21.675927546088644	28.033096361442965	28.603363580964867	21.68761251150352
40-41	21.93882017129713	27.926524561430956	28.496092677385526	21.638562589886394
42-43	21.770208303646626	28.024025046022512	28.387625367438048	21.818141282892814
44-45	21.894801120017995	28.10651485635575	28.19445463836223	21.804229385264023
46-47	21.873769281893676	28.08331020152296	28.04142932971734	22.001491186866026
48-49	21.886302768114934	28.146867635306304	28.01826725142083	21.94856234515793
50-51	21.81592793482576	28.279632152253974	27.932012074854445	21.972427838065823
52-53	22.01730711987413	28.201384853080835	27.83505081574508	21.946257211299947
54-55	21.960772775927666	28.13347945238428	27.913083933053535	21.992663838634517
56-57	21.964425611510332	28.195580837666434	27.870864208501768	21.969129342321462
58-59	21.96178160118367	28.247489279966935	27.87491795827725	21.915811160572144
60-61	22.009314381888473	28.23310718274029	27.848761448610116	21.90881698676112
62-63	21.9375694626471	28.268978674887833	27.84132858861629	21.952123273848777
64-65	21.981482566878487	28.22015968780901	27.837241707899494	21.961116037413003
66-67	22.007343169813364	28.24855563573215	27.68707131251703	22.057029881937456
68-69	21.968786836638614	28.275110995652447	27.7654475077803	21.99065465992864
70-71	21.965292417024806	28.074018540603912	27.792043819512667	22.16864522285861
72-73	21.96904437493224	28.119989115000525	27.83319111081642	22.07777539925082
74-75	21.91521828774132	28.22063456413345	27.7583874221146	22.105759726010625
76-77	21.971582817069468	28.143871781301723	27.77385527920545	22.11069012242336
78-79	21.973143027907465	28.17486685601568	27.70396478492903	22.148025331147824
80-81	21.948355801188306	28.206669026840974	27.739259618369307	22.105715553601417
82-83	21.996992146892264	28.18792031213392	27.798807148070477	22.016280392903344
84-85	22.10020135379166	28.161782339762485	27.725074525499917	22.012941780945933
86-87	21.938211490134247	28.179161351196775	27.828902592911298	22.053724565757683
88-89	21.995019628255708	28.164740553463496	27.760933738151493	22.079306080129303
90-91	22.024697827548103	28.19464241977193	27.758925621717683	22.02173413096228
92-93	21.999962748461883	28.16079753011452	27.810162906762837	22.02907681466076
94-95	21.90952918006543	28.235953642661816	27.830073120292038	22.02444405698071
96-97	21.89845615480587	28.22387186408644	27.88938067422816	21.988291306879525
98-99	22.191855845283065	28.764636129142673	27.940822239336928	21.102685786237334
100-101	26.105636052517546	35.788070002308956	28.6223791043171	9.483914840856398
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1812.0
1	1540.0
2	1933.0
3	2808.5
4	3954.0
5	6583.5
6	8802.5
7	10191.5
8	10876.0
9	10462.0
10	9316.5
11	8161.0
12	8558.0
13	10972.5
14	17073.5
15	25301.0
16	31716.5
17	35457.5
18	37317.0
19	37728.0
20	37375.0
21	37743.0
22	40396.5
23	46404.0
24	55887.0
25	68461.5
26	83538.5
27	98565.5
28	113504.0
29	129275.5
30	143321.0
31	158019.0
32	175556.5
33	192888.0
34	210218.0
35	227230.5
36	244168.5
37	259900.5
38	272868.5
39	284440.5
40	295508.0
41	305636.5
42	312474.5
43	317988.5
44	324525.5
45	329458.0
46	331566.0
47	332434.5
48	331446.5
49	326235.5
50	318739.0
51	308430.0
52	292962.5
53	277752.5
54	262168.0
55	242346.0
56	221793.5
57	199980.0
58	176107.0
59	153328.5
60	130626.0
61	107552.5
62	86446.5
63	68098.0
64	51915.5
65	39206.5
66	30141.0
67	23638.0
68	18137.5
69	13308.5
70	9805.0
71	7121.5
72	5187.0
73	3747.0
74	2657.5
75	1865.5
76	1283.0
77	867.0
78	597.5
79	439.5
80	316.5
81	214.0
82	160.5
83	116.0
84	86.5
85	65.0
86	44.5
87	31.5
88	22.0
89	16.0
90	12.5
91	11.0
92	10.0
93	7.0
94	3.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	8.505765168968958E-4
3	0.002165103861192099
4	5.799385342478835E-4
5	3.86625689498589E-4
6	3.86625689498589E-4
7	0.0
8	0.0
9	0.0
10-11	6.44376149164315E-6
12-13	9.85895508221402E-4
14-15	6.637074336392444E-4
16-17	9.665642237464725E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.158359376062904E-5
28-29	1.548684843924832E-4
30-31	1.9392639342739496E-5
32-33	0.001018053657782495
34-35	0.0015491322255953095
36-37	0.0011780983659775664
38-39	5.674202843778507E-4
40-41	1.9970127352164808E-5
42-43	1.3430365034635567E-5
44-45	5.969341732197151E-4
46-47	6.862341296348425E-6
48-49	0.0
50-51	0.0
52-53	8.433783354815856E-4
54-55	0.0032927959137261697
56-57	0.006172361122055655
58-59	0.004207559880162841
60-61	1.332167834496797E-4
62-63	0.0
64-65	4.537657680768371E-5
66-67	1.9122004738738726E-4
68-69	0.0
70-71	1.410537704025463E-4
72-73	7.931360106115253E-6
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	2.9814095086970347E-4
90-91	1.1569973284931685E-4
92-93	6.329104768022035E-4
94-95	4.0462821104671805E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	159.0
20-21	542.0
22-23	1157.0
24-25	2376.0
26-27	4608.0
28-29	10133.0
30-31	23493.0
32-33	27159.0
34-35	37117.0
36-37	57500.0
38-39	67857.0
40-41	64824.0
42-43	71248.0
44-45	83909.0
46-47	83527.0
48-49	79467.0
50-51	72352.0
52-53	68789.0
54-55	74170.0
56-57	77014.0
58-59	76754.0
60-61	75318.0
62-63	70789.0
64-65	72939.0
66-67	77551.0
68-69	78557.0
70-71	77540.0
72-73	73408.0
74-75	72786.0
76-77	72057.0
78-79	77893.0
80-81	79203.0
82-83	74631.0
84-85	73371.0
86-87	76940.0
88-89	82655.0
90-91	87574.0
92-93	90163.0
94-95	104148.0
96-97	153920.0
98-99	629445.0
100-101	4574400.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.4713704225924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.8790679210183	79.49316613779325
2	6.67775230358008	11.682242906735455
3	1.4838710870759577	3.8938871255098784
4	0.48819441905102057	1.708121394682165
5	0.19782743120947596	0.8652118257536994
6	0.08737294452868287	0.45855787174686224
7	0.05978698906346126	0.36607549067750506
8	0.03307720501010422	0.23146467615862842
9	0.020282886855923433	0.1596754718472608
>10	0.07085902649030817	0.9791198096939503
>50	0.0016353083170554256	0.09455433051413192
>100	2.2959549688306803E-4	0.03892793441592269
>500	4.2882302771166896E-5	0.028995024471292683
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.57750459665726E-5	0.0	1.28875229832863E-5	1.28875229832863E-5
3	0.0	2.57750459665726E-5	0.0	1.28875229832863E-5	1.28875229832863E-5
4	0.0	7.73251378997178E-5	0.0	1.28875229832863E-5	1.28875229832863E-5
5	0.0	7.73251378997178E-5	0.0	2.57750459665726E-5	1.28875229832863E-5
6	0.0	7.73251378997178E-5	0.0	2.57750459665726E-5	1.28875229832863E-5
7	0.0	7.73251378997178E-5	0.0	2.57750459665726E-5	1.28875229832863E-5
8	0.0	7.73251378997178E-5	0.0	2.57750459665726E-5	1.28875229832863E-5
9	0.0	1.675377987827219E-4	0.0	3.86625689498589E-5	1.28875229832863E-5
10-11	0.0	1.8042532176600821E-4	0.0	1.159877068495767E-4	1.28875229832863E-5
12-13	0.0	1.8042532176600821E-4	0.0	1.159877068495767E-4	1.28875229832863E-5
14-15	0.0	2.5130669817408286E-4	0.0	1.2243146834121986E-4	1.28875229832863E-5
16-17	0.0	2.57750459665726E-4	0.0	1.546502757994356E-4	1.28875229832863E-5
18-19	0.0	2.7708174414065544E-4	0.0	1.8686908325765136E-4	1.28875229832863E-5
20-21	0.0	3.093005515988712E-4	0.0	1.933128447492945E-4	2.57750459665726E-5
22-23	0.0	3.4151935905708694E-4	0.0	2.1908789071586708E-4	2.57750459665726E-5
24-25	0.0	3.86625689498589E-4	0.0	2.448629366824397E-4	2.57750459665726E-5
26-27	0.0	4.2528825844844793E-4	0.0	3.093005515988712E-4	2.57750459665726E-5
28-29	0.0	4.2528825844844793E-4	0.0	7.73251378997178E-4	2.57750459665726E-5
30-31	0.0	4.639508273983068E-4	0.0	0.0018171407406433683	2.57750459665726E-5
32-33	0.0	4.639508273983068E-4	0.0	0.0038984757024441057	3.86625689498589E-5
34-35	6.44376149164315E-6	5.477197267896678E-4	0.0	0.006611299290425872	3.86625689498589E-5
36-37	1.28875229832863E-5	6.057135802144561E-4	0.0	0.01078041297551899	3.86625689498589E-5
38-39	1.28875229832863E-5	6.44376149164315E-4	0.0	0.01728861208207857	3.86625689498589E-5
40-41	1.28875229832863E-5	7.474763330306054E-4	0.0	0.02438319348437768	3.86625689498589E-5
42-43	1.28875229832863E-5	7.73251378997178E-4	0.0	0.030504766901438676	3.86625689498589E-5
44-45	1.28875229832863E-5	8.699078013718253E-4	0.0	0.0373029352751222	3.86625689498589E-5
46-47	1.28875229832863E-5	9.536767007631862E-4	0.0	0.04502256154211069	3.86625689498589E-5
48-49	1.28875229832863E-5	9.601204622548293E-4	0.0	0.05206559285247665	3.86625689498589E-5
50-51	1.28875229832863E-5	0.0010567768846294766	0.0	0.05964990012814064	3.86625689498589E-5
52-53	1.28875229832863E-5	0.0010567768846294766	0.0	0.06658338749314867	3.86625689498589E-5
54-55	1.28875229832863E-5	0.0010632206461211199	0.0	0.07357486871158148	3.86625689498589E-5
56-57	1.28875229832863E-5	0.0010696644076127629	0.0	0.08036014956228173	3.86625689498589E-5
58-59	1.28875229832863E-5	0.0011018832150709785	0.0	0.0868232423383998	3.86625689498589E-5
60-61	1.28875229832863E-5	0.0011534333070041239	0.0	0.09385982988727412	3.86625689498589E-5
62-63	1.28875229832863E-5	0.0012114271604289123	0.0	0.10011027853416798	3.86625689498589E-5
64-65	1.28875229832863E-5	0.001282308536836987	0.0	0.10608364543692118	3.86625689498589E-5
66-67	1.28875229832863E-5	0.0013016398213119163	0.0	0.1123598691297816	3.86625689498589E-5
68-69	1.28875229832863E-5	0.0013209711057868456	0.0	0.11897116842020748	3.86625689498589E-5
70-71	1.28875229832863E-5	0.0013660774362283478	0.0	0.1255180300957169	3.86625689498589E-5
72-73	1.28875229832863E-5	0.0013854087207032773	0.0	0.13078902699588102	3.86625689498589E-5
74-75	1.28875229832863E-5	0.0014176275281614931	0.0	0.13501613453439892	3.86625689498589E-5
76-77	1.28875229832863E-5	0.0015013964275528541	0.0	0.13916591693501712	3.86625689498589E-5
78-79	1.28875229832863E-5	0.001546502757994356	0.0	0.14273576080138742	3.86625689498589E-5
80-81	1.28875229832863E-5	0.001546502757994356	0.0	0.14442402631219792	3.86625689498589E-5
82-83	1.28875229832863E-5	0.0015658340424692854	0.0	0.1450941775073288	3.86625689498589E-5
84-85	1.28875229832863E-5	0.001591609088435858	0.0	0.1457063348490349	3.86625689498589E-5
86-87	1.28875229832863E-5	0.00163671541887736	0.0	0.14589320393229255	3.86625689498589E-5
88-89	1.28875229832863E-5	0.0017011530337937917	0.0	0.1459898603546672	3.86625689498589E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7940	0.0	44.869556	1
GTATCAA	13325	0.0	32.340557	1
TCAACGC	15155	0.0	27.526087	4
CAACGCA	15420	0.0	27.134197	5
ATCAACG	15380	0.0	27.09697	3
AACGCAG	15995	0.0	26.185007	6
TATCAAC	16715	0.0	25.282352	2
ACGCAGA	18905	0.0	22.066006	7
GTACATG	19745	0.0	21.973064	1
CGCAGAG	19035	0.0	21.827644	8
TACATGG	19505	0.0	21.580406	2
ACATGGG	19775	0.0	20.694939	3
CATGGGG	14040	0.0	18.688908	4
GAGTACT	14255	0.0	17.441324	12-13
GCAGAGT	23585	0.0	17.422222	9
CAGAGTA	22955	0.0	16.528316	10-11
AGAGTAC	20940	0.0	16.345758	10-11
GTACTTT	16275	0.0	15.302736	14-15
GAGTACA	13820	0.0	15.153399	1
AGTACTT	15680	0.0	14.353103	12-13
>>END_MODULE