FastQCFastQC Report
Fri 27 May 2016
SRR2138813_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138813_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9175579
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92800.043.6940771
CTTATAC85450.035.195531
GTATCAA161450.033.3216131
TATACAC118500.028.5383473
TCAACGC183350.028.4688684
ATCAACG185950.028.0489543
CAACGCA188700.027.5055435
AACGCAG194550.026.8080396
TATCAAC201200.026.2785132
TTATACA131900.024.140412
ACGCAGA225150.023.052367
CGCAGAG228650.022.6810998
GTACATG246000.022.6726321
TACATGG247050.021.9803662
ACATGGG249200.021.2842433
ACATCTC140900.021.1644738
ACACATC143200.021.1477436
TACACAT162750.020.5716445
CATGGGG178800.019.7366644
CACATCT158850.019.0111967
Produced by FastQC (version 0.11.5)