##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138813_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9175579
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45673411999395	34.0	31.0	34.0	31.0	34.0
2	32.655239631199294	34.0	31.0	34.0	31.0	34.0
3	32.73889200888576	34.0	31.0	34.0	31.0	34.0
4	36.19676534854095	37.0	37.0	37.0	35.0	37.0
5	36.01047410741055	37.0	35.0	37.0	35.0	37.0
6	36.07295942850037	37.0	35.0	37.0	35.0	37.0
7	36.076402371992	37.0	35.0	37.0	35.0	37.0
8	36.06378137009119	37.0	35.0	37.0	35.0	37.0
9	37.934543858213196	39.0	38.0	39.0	35.0	39.0
10-11	37.81154971255765	39.0	38.0	39.0	35.0	39.0
12-13	37.81416219074568	39.0	38.0	39.0	35.0	39.0
14-15	39.144086057130565	40.0	39.0	41.0	36.0	41.0
16-17	39.118486473714626	40.0	38.5	41.0	36.0	41.0
18-19	39.189251163332585	40.0	39.0	41.0	36.0	41.0
20-21	39.202283996193856	40.0	39.0	41.0	36.0	41.0
22-23	39.163688527434644	40.0	39.0	41.0	36.0	41.0
24-25	39.13178931867617	40.0	39.0	41.0	36.0	41.0
26-27	39.040450939121314	40.0	39.0	41.0	36.0	41.0
28-29	38.98154308197685	40.0	39.0	41.0	35.5	41.0
30-31	38.85355618240927	40.0	38.0	41.0	35.0	41.0
32-33	38.7312003356099	40.0	38.0	41.0	35.0	41.0
34-35	38.69552379794338	40.0	38.0	41.0	35.0	41.0
36-37	38.64664384607313	40.0	38.0	41.0	35.0	41.0
38-39	38.572283565406885	40.0	38.0	41.0	35.0	41.0
40-41	38.496914129659686	40.0	38.0	41.0	34.0	41.0
42-43	38.519094007634095	40.0	38.0	41.0	34.0	41.0
44-45	38.462542575489955	40.0	38.0	41.0	34.0	41.0
46-47	38.41833695018077	40.0	38.0	41.0	34.0	41.0
48-49	38.37641899841336	40.0	38.0	41.0	34.0	41.0
50-51	38.30245434080756	40.0	38.0	41.0	34.0	41.0
52-53	38.06014382620219	40.0	37.0	41.0	33.0	41.0
54-55	37.91290061573848	40.0	37.0	41.0	33.0	41.0
56-57	37.82000106099369	39.5	37.0	41.0	33.0	41.0
58-59	37.69648164065437	39.0	36.0	41.0	33.0	41.0
60-61	37.514353275470555	39.0	36.0	41.0	33.0	41.0
62-63	37.27521307088371	39.0	35.5	40.5	32.0	41.0
64-65	37.05186996036812	39.0	35.0	40.0	32.0	41.0
66-67	36.79885928146116	38.0	35.0	40.0	32.0	41.0
68-69	36.5680432172856	37.5	35.0	40.0	31.5	41.0
70-71	36.260233058561305	37.0	35.0	39.5	31.0	41.0
72-73	35.96825751320756	36.5	35.0	39.0	31.0	41.0
74-75	35.52181256295801	36.0	34.0	39.0	31.0	40.0
76-77	33.59382270414742	34.5	32.0	36.5	28.0	39.0
78-79	34.76841685048083	35.0	34.0	37.0	30.5	39.0
80-81	34.734101517116386	35.0	34.0	37.0	31.0	39.0
82-83	34.461818777969256	35.0	34.0	36.5	31.0	39.0
84-85	34.178446663082866	35.0	34.0	36.0	30.0	37.0
86-87	33.9464124958225	35.0	34.0	36.0	30.0	37.0
88-89	33.80690261895144	35.0	34.0	35.0	30.0	36.5
90-91	33.63745532485814	35.0	34.0	35.0	30.0	36.0
92-93	33.59670883663526	35.0	34.0	35.0	30.0	36.0
94-95	33.46778786757993	35.0	33.0	35.0	30.0	36.0
96-97	33.48784320548243	35.0	33.5	35.0	30.0	35.5
98-99	33.58077051168387	35.0	33.5	35.0	30.0	35.0
100-101	32.307643533040846	34.0	31.5	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	13.0
20	27.0
21	142.0
22	733.0
23	2385.0
24	6272.0
25	13584.0
26	25133.0
27	41662.0
28	62375.0
29	87267.0
30	117425.0
31	154176.0
32	205898.0
33	282641.0
34	417244.0
35	648766.0
36	1181865.0
37	2219090.0
38	2628236.0
39	1063428.0
40	17213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.165380408146454	20.482304168488984	13.87399094923601	24.478324474128556
2	14.267687982788042	21.154237036728645	42.25540873552024	22.32266624496307
3	17.983810312569105	26.54570993336819	30.623800930907574	24.846678823155134
4	12.275040439045645	18.40503521428389	39.03453944607935	30.28538490059112
5	12.960199783053946	38.033914686123005	35.99505119745916	13.010834333363885
6	29.357656684153365	38.45496915987007	19.12506559555863	13.062308560417938
7	25.967941641611937	32.619827043067254	23.611218431011274	17.801012884309536
8	23.859246375623815	37.464709311532275	21.606712775291893	17.069331537552017
9	25.30617413898349	16.722443346626953	21.290623730665935	36.68075878372362
10-11	23.86329516644127	26.846403916308713	29.459448826063184	19.830852091186834
12-13	24.74403968091311	24.306008793313538	29.312131351612713	21.63782017416064
14-15	21.885611294192014	25.767835856580216	26.647704964709057	25.69884788451871
16-17	21.727570192283014	28.94178106881392	27.306797015790913	22.023851723112156
18-19	21.819320611810983	28.14989114038471	28.705289333784823	21.325498914019487
20-21	22.478382303872394	27.427552605798912	28.474794081563914	21.619271008764787
22-23	22.10142800908142	27.58027050135884	28.371327812957496	21.94697367660224
24-25	22.046174961789603	27.60131472444169	28.463461461203586	21.889048852565125
26-27	22.04552803677927	27.783542791457794	28.388916487036354	21.782012684726578
28-29	21.819507978391822	27.727142497109973	28.447140449934473	22.006209074563728
30-31	22.161630422133214	27.641150434983576	28.41452680682227	21.78269233606094
32-33	21.795282077800852	27.626686723195252	28.61149258808649	21.966538610917404
34-35	21.829685548403187	27.737498417907346	28.489923272009964	21.9428927616795
36-37	21.92557623731939	27.71724736460769	28.500261067140443	21.856915330932484
38-39	21.868240501873174	27.685019701292862	28.513360165073244	21.933379631760722
40-41	22.047853200358908	27.54000956380478	28.47774426430838	21.93439297152793
42-43	22.0806464455715	27.584362016748447	28.386229656690148	21.94876188098991
44-45	21.98232544370908	27.883092391352974	28.17508841296204	21.9594937519759
46-47	22.210977080049087	27.596433221623613	27.864308481255556	22.328281217071748
48-49	22.116795707688524	27.661275769967585	27.94315045220464	22.278778070139246
50-51	22.00850004934371	27.833444315049043	27.944251657510797	22.213803978096447
52-53	22.144131686078577	27.84434262546105	27.957783880376823	22.05374180808355
54-55	22.089798643468118	27.78142237182432	27.82833279053845	22.300446194169112
56-57	22.276700824465596	27.964809605128966	27.59404902989404	22.1644405405114
58-59	22.021308606140323	28.17087511004942	27.891007152201563	21.91680913160869
60-61	22.295013240227384	28.26656528345689	27.4908139027222	21.947607573593526
62-63	22.1309905153068	28.331630720967237	27.46185927778676	22.0755194859392
64-65	22.14625218661435	28.19066166989934	27.545476027728572	22.11761011575774
66-67	22.227382576816215	28.128210950729116	27.40771856042547	22.2366879120292
68-69	22.216353183244042	27.987492226702837	27.567945543411515	22.228209046641602
70-71	22.228466515421857	27.748704638154393	27.640643492508854	22.382185353914892
72-73	22.26169685236767	27.815840988554726	27.608063935455927	22.314398223621676
74-75	22.18326494570037	27.925234696650293	27.572734540323847	22.318765817325495
76-77	22.225704631332572	27.911347152491782	27.505082982276768	22.357865233898874
78-79	22.22731842089188	27.930006052768935	27.460198516221094	22.38247701011809
80-81	22.191957432818523	27.915537809158202	27.51760355302925	22.374901204994018
82-83	22.265213787013728	27.85895769398868	27.60997210652414	22.265856412473457
84-85	22.440587871441085	27.83003139649637	27.468924503422613	22.26045622863993
86-87	22.19594006974361	27.898537428253377	27.579952283887206	22.325570218115807
88-89	22.26894060028597	27.87422667739107	27.49617822070631	22.360654501616654
90-91	22.315076194505185	27.887103321438232	27.521447087262867	22.276373396793716
92-93	22.312934906157825	27.84024334417795	27.537899356501725	22.308922393162497
94-95	22.132053764863464	27.94304820094105	27.562867068060743	22.36203096613474
96-97	22.177722633366827	27.921913528477667	27.629244060001163	22.27111977815435
98-99	22.416838534634074	28.534674081749845	27.64819218359263	21.40029520002345
100-101	23.170308809015005	31.1223206320226	24.70588823715963	21.001482321802765
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1375.0
1	1315.0
2	2050.5
3	3035.0
4	4263.5
5	6987.5
6	9075.5
7	10424.5
8	11301.0
9	11003.5
10	9953.0
11	8916.5
12	9544.0
13	12429.5
14	19071.0
15	27919.0
16	34969.5
17	38999.5
18	39850.5
19	38561.5
20	36799.0
21	35647.5
22	36898.0
23	42546.0
24	52602.5
25	66922.0
26	85750.0
27	106433.0
28	127204.5
29	149075.5
30	167597.5
31	185086.0
32	205681.5
33	226217.0
34	246154.0
35	265072.0
36	287264.0
37	308058.0
38	322035.5
39	332921.0
40	345649.5
41	358544.0
42	366832.5
43	374279.5
44	383051.5
45	390178.5
46	392892.5
47	396577.0
48	405804.5
49	402715.5
50	389757.5
51	378513.5
52	362794.5
53	342462.0
54	317222.5
55	289084.0
56	263029.0
57	237086.5
58	209076.0
59	180925.0
60	151680.5
61	122907.5
62	96423.0
63	73913.0
64	55014.0
65	41056.5
66	31021.0
67	23347.5
68	17653.5
69	12646.5
70	8756.0
71	6099.5
72	4229.0
73	2990.0
74	2082.0
75	1415.5
76	974.0
77	664.5
78	472.0
79	329.5
80	229.0
81	162.5
82	124.5
83	91.5
84	64.5
85	42.5
86	31.0
87	26.0
88	17.0
89	9.5
90	5.0
91	3.0
92	2.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0010680524902025256
3	0.0025284507931325096
4	4.4683828671738315E-4
5	2.8336086474760883E-4
6	3.705488231314885E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	9.209228104297288E-4
14-15	7.247499040659996E-4
16-17	1.689266693687668E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.179466213487885E-5
28-29	2.1279230979504826E-4
30-31	4.9172269528070235E-5
32-33	0.0012106079273126898
34-35	0.0020169041850706883
36-37	0.001402350084773167
38-39	8.283391616351549E-4
40-41	6.162963547303211E-5
42-43	5.0824718590592154E-5
44-45	7.124166586496046E-4
46-47	3.455710604971097E-5
48-49	1.1638334789386002E-5
50-51	0.0
52-53	0.0011392056497005522
54-55	0.0038341656268489365
56-57	0.0069468868407854085
58-59	0.004943250322459796
60-61	2.975044582282667E-4
62-63	2.508298234402602E-5
64-65	1.6493745603385937E-4
66-67	1.7981291750311784E-4
68-69	0.0
70-71	1.6449638904106632E-4
72-73	6.655893880557854E-6
74-75	0.0
76-77	0.0
78-79	6.8867544847233885E-6
80-81	0.0
82-83	0.0
84-85	7.1492153485932384E-6
86-87	2.8953065127097084E-5
88-89	3.3739805077811325E-4
90-91	1.5626946391983644E-4
92-93	5.742923471464245E-4
94-95	3.0732167774894247E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	178.0
20-21	575.0
22-23	1324.0
24-25	3247.0
26-27	4531.0
28-29	8929.0
30-31	22929.0
32-33	27529.0
34-35	36940.0
36-37	57713.0
38-39	70445.0
40-41	69224.0
42-43	77136.0
44-45	90605.0
46-47	89954.0
48-49	87119.0
50-51	80900.0
52-53	78822.0
54-55	88661.0
56-57	92883.0
58-59	94460.0
60-61	95292.0
62-63	91046.0
64-65	93440.0
66-67	101249.0
68-69	89486.0
70-71	87692.0
72-73	84315.0
74-75	83842.0
76-77	82550.0
78-79	88893.0
80-81	91346.0
82-83	87367.0
84-85	85343.0
86-87	89325.0
88-89	95802.0
90-91	102020.0
92-93	105522.0
94-95	122872.0
96-97	180957.0
98-99	755425.0
100-101	5477691.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.57255487359394
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.33022084248688	75.54885004071487
2	7.360774463591261	12.450390044684418
3	1.8402339348767067	4.6689985631283
4	0.6864309942204635	2.322128917025832
5	0.31849264383985965	1.3467868298991272
6	0.162413952634909	0.8241457752871877
7	0.09321362182209215	0.5518319902560725
8	0.054176061021042325	0.36654463148298205
9	0.03457895948786539	0.263198785388335
>10	0.11718494437966745	1.4788110298110122
>50	0.0018486128717587456	0.10060702816416048
>100	3.892950870842733E-4	0.050585876538478895
>500	4.167368042449055E-5	0.02712048761918759
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0898494797984955E-5
2	0.0	4.359397919193982E-5	0.0	0.0	1.0898494797984955E-5
3	0.0	4.359397919193982E-5	0.0	1.0898494797984955E-5	1.0898494797984955E-5
4	0.0	5.4492473989924776E-5	0.0	2.179698959596991E-5	2.179698959596991E-5
5	0.0	5.4492473989924776E-5	0.0	2.179698959596991E-5	2.179698959596991E-5
6	0.0	5.4492473989924776E-5	0.0	2.179698959596991E-5	2.179698959596991E-5
7	0.0	5.4492473989924776E-5	0.0	2.179698959596991E-5	2.179698959596991E-5
8	0.0	5.4492473989924776E-5	0.0	2.179698959596991E-5	2.179698959596991E-5
9	0.0	9.80864531818646E-5	0.0	3.269548439395487E-5	2.179698959596991E-5
10-11	0.0	9.80864531818646E-5	0.0	5.4492473989924776E-5	2.179698959596991E-5
12-13	0.0	9.80864531818646E-5	0.0	5.4492473989924776E-5	2.179698959596991E-5
14-15	0.0	1.3078193757581948E-4	0.0	5.4492473989924776E-5	3.8144731792947345E-5
16-17	0.0	1.3078193757581948E-4	0.0	6.539096878790973E-5	5.4492473989924776E-5
18-19	0.0	1.471296797727969E-4	0.0	9.80864531818646E-5	5.994172138891726E-5
20-21	0.0	1.6347742196977434E-4	0.0	1.0898494797984955E-4	6.539096878790974E-5
22-23	1.0898494797984955E-5	1.7982516416675175E-4	0.0	1.25332690176827E-4	7.628946358589469E-5
24-25	2.179698959596991E-5	2.288683907576841E-4	0.0	1.3623118497481195E-4	8.718795838387964E-5
26-27	2.7246236994962388E-5	2.6156387515163896E-4	0.0	1.9617290636372918E-4	8.718795838387964E-5
28-29	3.269548439395487E-5	2.724623699496239E-4	0.0	5.122292555052929E-4	9.263720578287212E-5
30-31	3.269548439395487E-5	3.4875183353551856E-4	0.0	0.0016947159410866605	1.0353570058085708E-4
32-33	3.269548439395487E-5	3.4875183353551856E-4	0.0	0.004397542650986929	1.3623118497481195E-4
34-35	3.8144731792947345E-5	4.413890393183907E-4	0.0	0.008043089160912897	1.5257892717178938E-4
36-37	4.359397919193982E-5	5.394754925002553E-4	0.0	0.012991005799198065	1.6347742196977434E-4
38-39	4.359397919193982E-5	5.885187190911875E-4	0.0	0.02036928677743388	1.7437591676775928E-4
40-41	4.359397919193982E-5	6.430111930811124E-4	0.0	0.02878837400887726	1.7982516416675175E-4
42-43	4.359397919193982E-5	6.484604404801049E-4	0.0	0.03569801971079972	1.9617290636372918E-4
44-45	4.359397919193982E-5	6.757066774750672E-4	0.0	0.04308174993643453	2.3976688555566903E-4
46-47	4.9043226590932295E-5	7.029529144700296E-4	0.0	0.05096681092277665	2.5066538035365397E-4
48-49	5.4492473989924776E-5	7.084021618690221E-4	0.0	0.05878648094033085	2.5066538035365397E-4
50-51	5.4492473989924776E-5	8.010393676518943E-4	0.0	0.06697125053361755	2.5066538035365397E-4
52-53	7.084021618690222E-5	8.173871098488717E-4	0.0	0.07445306721243422	2.5066538035365397E-4
54-55	7.628946358589469E-5	8.282856046468567E-4	0.0	0.08131911893516475	2.5066538035365397E-4
56-57	7.628946358589469E-5	8.391840994448416E-4	0.0	0.08844673453304691	2.5066538035365397E-4
58-59	7.628946358589469E-5	8.44633346843834E-4	0.0	0.094893194206055	2.5066538035365397E-4
60-61	7.628946358589469E-5	8.609810890408114E-4	0.0	0.1012034226940883	2.5066538035365397E-4
62-63	7.628946358589469E-5	8.718795838387964E-4	0.0	0.10702866816361126	2.5066538035365397E-4
64-65	7.628946358589469E-5	9.427198000256986E-4	0.0	0.11270133470596241	2.5066538035365397E-4
66-67	7.628946358589469E-5	9.590675422226761E-4	0.0	0.1185102324332884	2.5611462775264646E-4
68-69	8.718795838387964E-5	9.863137792176383E-4	0.0	0.12441721661379626	2.779116173486164E-4
70-71	8.718795838387964E-5	0.0010026615214146159	0.0	0.13045498273187994	2.942593595455938E-4
72-73	8.718795838387964E-5	0.0010244585110105859	0.0	0.1351903787216044	3.160563491415637E-4
74-75	8.718795838387964E-5	0.0010517047480055482	0.0	0.13934270523963666	3.160563491415637E-4
76-77	8.718795838387964E-5	0.0011007479745964804	0.0	0.14304274422355256	3.160563491415637E-4
78-79	8.718795838387964E-5	0.0011334434589904353	0.0	0.1462632494363571	3.160563491415637E-4
80-81	9.263720578287212E-5	0.0011334434589904353	0.0	0.14762011203870623	3.160563491415637E-4
82-83	9.80864531818646E-5	0.0011334434589904353	0.0	0.1482140800051964	3.160563491415637E-4
84-85	9.80864531818646E-5	0.0011443419537884203	0.0	0.14869906302370672	3.160563491415637E-4
86-87	9.80864531818646E-5	0.0011661389433843903	0.0	0.14888978668267147	3.215055965405562E-4
88-89	9.80864531818646E-5	0.00120973292257633	0.0	0.1490151193728483	3.378533387375336E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9280	0.0	43.694077	1
CTTATAC	8545	0.0	35.19553	1
GTATCAA	16145	0.0	33.321613	1
TATACAC	11850	0.0	28.538347	3
TCAACGC	18335	0.0	28.468868	4
ATCAACG	18595	0.0	28.048954	3
CAACGCA	18870	0.0	27.505543	5
AACGCAG	19455	0.0	26.808039	6
TATCAAC	20120	0.0	26.278513	2
TTATACA	13190	0.0	24.14041	2
ACGCAGA	22515	0.0	23.05236	7
CGCAGAG	22865	0.0	22.681099	8
GTACATG	24600	0.0	22.672632	1
TACATGG	24705	0.0	21.980366	2
ACATGGG	24920	0.0	21.284243	3
ACATCTC	14090	0.0	21.164473	8
ACACATC	14320	0.0	21.147743	6
TACACAT	16275	0.0	20.571644	5
CATGGGG	17880	0.0	19.736664	4
CACATCT	15885	0.0	19.011196	7
>>END_MODULE