FastQCFastQC Report
Fri 27 May 2016
SRR2138812_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138812_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9734599
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133250.035.2306181
GTATCAA211100.031.6132971
TCAACGC232300.027.701224
ATCAACG232600.027.6480463
CAACGCA236200.027.2255345
AACGCAG245550.026.3459196
TATCAAC260450.025.2310222
ACGCAGA284700.022.6333147
CGCAGAG288950.022.270958
GTACATG268550.021.6806771
TACATGG261250.021.5533832
ACATGGG267900.020.6052043
TATACCG8850.018.7548315
CATGGGG183800.018.5242564
GCAGAGT346350.018.4453269
AGAGTAC289350.017.95292310-11
GAGTACT184850.017.82105612-13
CAGAGTA323650.016.78015310-11
AGTACTT207400.015.93472812-13
GTACTTT212000.015.54930214-15
Produced by FastQC (version 0.11.5)