##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138812_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9734599
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.459305925185	34.0	31.0	34.0	31.0	34.0
2	32.648619835290596	34.0	31.0	34.0	31.0	34.0
3	32.728693703767355	34.0	31.0	34.0	31.0	34.0
4	36.18583908797887	37.0	35.0	37.0	35.0	37.0
5	36.00405738336011	37.0	35.0	37.0	35.0	37.0
6	36.07187763974664	37.0	35.0	37.0	35.0	37.0
7	36.07450096300834	37.0	35.0	37.0	35.0	37.0
8	36.06328190817105	37.0	35.0	37.0	35.0	37.0
9	37.92282907595886	39.0	38.0	39.0	35.0	39.0
10-11	37.80010126765366	39.0	38.0	39.0	35.0	39.0
12-13	37.80448773493392	39.0	38.0	39.0	35.0	39.0
14-15	39.13358043818754	40.0	39.0	41.0	36.0	41.0
16-17	39.11030361908077	40.0	38.5	41.0	36.0	41.0
18-19	39.18105825417154	40.0	39.0	41.0	36.0	41.0
20-21	39.197742885320736	40.0	39.0	41.0	36.0	41.0
22-23	39.16171832561778	40.0	39.0	41.0	36.0	41.0
24-25	39.13017173793331	40.0	39.0	41.0	36.0	41.0
26-27	39.04203532391123	40.0	39.0	41.0	36.0	41.0
28-29	38.980494776461754	40.0	38.5	41.0	35.5	41.0
30-31	38.850903045289485	40.0	38.0	41.0	35.0	41.0
32-33	38.72063587228194	40.0	38.0	41.0	35.0	41.0
34-35	38.68439780493892	40.0	38.0	41.0	35.0	41.0
36-37	38.63677336175927	40.0	38.0	41.0	35.0	41.0
38-39	38.57029948224424	40.0	38.0	41.0	35.0	41.0
40-41	38.48919914074091	40.0	38.0	41.0	34.0	41.0
42-43	38.504991801197434	40.0	38.0	41.0	34.0	41.0
44-45	38.44956388283417	40.0	38.0	41.0	34.0	41.0
46-47	38.4031478695716	40.0	38.0	41.0	34.0	41.0
48-49	38.36001716003787	40.0	38.0	41.0	34.0	41.0
50-51	38.30186420115511	40.0	38.0	41.0	34.0	41.0
52-53	38.05294129578711	40.0	37.0	41.0	33.0	41.0
54-55	37.90757377473814	40.0	37.0	41.0	33.0	41.0
56-57	37.81991342607848	39.5	37.0	41.0	33.0	41.0
58-59	37.694189084941975	39.0	36.5	41.0	33.0	41.0
60-61	37.5187443885118	39.0	36.0	41.0	33.0	41.0
62-63	37.27008423337794	39.0	35.5	40.0	32.0	41.0
64-65	37.03533662458564	39.0	35.0	40.0	32.0	41.0
66-67	36.77328021154949	38.0	35.0	40.0	32.0	41.0
68-69	36.5343595500043	37.5	35.0	40.0	31.5	41.0
70-71	36.22716167941227	37.0	35.0	39.5	31.0	41.0
72-73	35.931247814002504	36.5	35.0	39.0	31.0	41.0
74-75	35.48333356413161	36.0	34.0	39.0	31.0	40.0
76-77	33.5555369555353	34.5	32.0	36.5	28.0	39.0
78-79	34.7373261029699	35.0	34.0	37.0	30.0	39.0
80-81	34.705033205879104	35.0	34.0	37.0	31.0	39.0
82-83	34.433425447013676	35.0	34.0	36.5	30.5	39.0
84-85	34.1493029660047	35.0	34.0	36.0	30.0	37.0
86-87	33.91613042230354	35.0	34.0	36.0	30.0	37.0
88-89	33.77611859030121	35.0	34.0	35.0	30.0	37.0
90-91	33.60703520591866	35.0	34.0	35.0	30.0	36.0
92-93	33.568615691785645	35.0	34.0	35.0	30.0	36.0
94-95	33.43574207371844	35.0	33.0	35.0	30.0	36.0
96-97	33.45668823012472	35.0	33.0	35.0	30.0	35.5
98-99	33.55278461582846	35.0	33.0	35.0	30.0	35.0
100-101	32.283455812504066	34.0	31.5	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	10.0
20	40.0
21	173.0
22	706.0
23	2555.0
24	6678.0
25	14792.0
26	27113.0
27	45329.0
28	67399.0
29	95056.0
30	127671.0
31	168410.0
32	222471.0
33	304967.0
34	447568.0
35	702088.0
36	1275176.0
37	2368368.0
38	2769077.0
39	1072246.0
40	16702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.82043862310096	20.21746350311913	14.019971444124202	23.942126429655705
2	14.506526836866113	21.100783697079077	42.0710522295904	22.321637236464404
3	18.406474619490364	26.127891671363933	30.312740032004097	25.152893677141602
4	12.43431394242494	18.056980889330205	39.24059449207884	30.268110676166017
5	13.095742067875479	37.98352077715677	35.810418158558974	13.110318996408774
6	29.702736974633844	38.36604981188641	18.937733498353904	12.993479715125844
7	26.41192513425566	32.56164943209268	23.47342710264696	17.552998331004698
8	23.954032415716352	37.55013431986258	21.24036131329087	17.25547195113019
9	25.36552353106687	17.007017957288227	21.376977110202485	36.25048140144242
10-11	23.985851908229606	26.966806747766398	29.285145695266955	19.76219564873705
12-13	24.83557450305614	24.393923673532285	29.343674559556217	21.426827263855362
14-15	21.92136560625132	25.910879623328075	26.868800605350916	25.298954165069688
16-17	21.519213209922576	29.042245891879492	27.522105062138458	21.916435836059478
18-19	21.555931579718898	28.369961618347094	29.037123152170928	21.036983649763076
20-21	22.397794564641483	27.53736914922824	28.84564186895956	21.219194417170716
22-23	22.039000557793706	27.437475784674163	28.756001881847393	21.767521775684738
24-25	21.8288141238455	27.57607581185402	28.819606481369675	21.775503582930803
26-27	21.826967278257076	27.706556268858666	28.745136474285232	21.721339978599023
28-29	21.734481575608132	27.841758182975006	28.757435619685122	21.666324621731736
30-31	22.04771895996399	27.705487247785705	28.615214899093683	21.63157889315662
32-33	21.832345250688586	27.853296415914414	28.587606220346938	21.72675211305006
34-35	21.92148320783135	27.87210007119496	28.55853526941243	21.647881451561254
36-37	21.952089277487698	27.7501785488405	28.582174293095026	21.71555788057678
38-39	21.766575044618868	27.917307183223294	28.641037495855354	21.675080276302484
40-41	22.0318661274228	27.78097385352573	28.51511572965707	21.672044289394396
42-43	21.904792615104192	27.899738076642677	28.352506007117213	21.842963301135914
44-45	21.97091197541727	27.979136168758977	28.18466612799955	21.86528572782421
46-47	22.010002776869015	27.93854792107989	28.007553818173296	22.043895483877797
48-49	22.0282467240397	27.98404204569307	28.026572965251784	21.961138265015443
50-51	21.97565835281707	28.13657358317186	27.919904856320404	21.967863207690666
52-53	22.14573056620111	28.053495143013457	27.798110994993507	22.002663295791926
54-55	22.11440610309701	27.957590453331584	27.820674474974528	22.107328968596878
56-57	22.073121587749757	28.09282980461037	27.810116830457687	22.02393177718218
58-59	22.075997046051214	28.103296110341546	27.791761428172002	22.028945415435235
60-61	22.14625236029692	28.04710829571905	27.80934813658165	21.997291207402384
62-63	22.101674346037708	28.070304227611075	27.76996315109908	22.058058275252137
64-65	22.06146242881626	28.073889252280576	27.780890051742286	22.08375826716088
66-67	22.18676486586016	28.1091028583205	27.59957163131078	22.10456064450856
68-69	22.103715027628546	28.097413651490616	27.678220903855845	22.120650417024994
70-71	22.110955460951207	27.855183778943033	27.759833280787298	22.274027479318462
72-73	22.16849406085688	27.896744999153277	27.727333596106014	22.20742734388383
74-75	22.07926592416575	28.023126958965626	27.658824703643326	22.238782413225294
76-77	22.112655682788642	28.031687751850924	27.65552965317093	22.200126912189504
78-79	22.115850549274274	28.04110702681271	27.55966326806828	22.28337915584473
80-81	22.12292583396133	28.014999441485728	27.595408814905436	22.26666590964751
82-83	22.146571516771324	27.977263387409778	27.68794301335	22.188222082468894
84-85	22.28494566077676	27.953603085186913	27.622534256200698	22.138916997835626
86-87	22.098410339643674	27.975012166610753	27.70003288916009	22.22654460458548
88-89	22.13564633523809	28.00637728050333	27.64429333081775	22.213683053440835
90-91	22.167869078218068	28.04148129940753	27.652454227241673	22.13819539513273
92-93	22.15566698443399	28.013229215780072	27.633808148839	22.197295650946934
94-95	22.02000511983348	28.059352299098993	27.677770830098464	22.242871750969062
96-97	22.058341698013116	28.094790068052383	27.730286397982262	22.116581835952235
98-99	22.29347440726213	28.635071559657288	27.78695077548	21.28450325760058
100-101	23.04380553263447	31.236952913034187	24.893216554965143	20.8260249993662
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2597.0
1	2196.5
2	2562.0
3	3560.5
4	4806.0
5	7589.5
6	9995.0
7	11845.5
8	13005.5
9	12729.0
10	11505.0
11	10318.0
12	11131.0
13	14523.0
14	22280.0
15	32548.0
16	40590.5
17	45215.5
18	46258.0
19	44336.5
20	41703.0
21	39863.5
22	40879.0
23	46472.5
24	56782.0
25	71986.5
26	92246.0
27	114182.0
28	135483.5
29	157270.5
30	176054.5
31	194419.5
32	215045.0
33	235931.5
34	257903.0
35	279065.5
36	302082.0
37	322632.5
38	337155.5
39	349834.5
40	363643.0
41	377000.0
42	386345.0
43	394477.0
44	403327.5
45	412193.5
46	418163.5
47	420715.0
48	418290.0
49	408204.0
50	395414.0
51	381286.0
52	361482.0
53	338873.5
54	315877.5
55	291573.5
56	266671.5
57	240362.5
58	213671.5
59	186572.0
60	157088.0
61	127857.5
62	101545.5
63	78707.0
64	59005.5
65	43659.0
66	32853.0
67	25092.5
68	19042.0
69	13693.5
70	9637.0
71	7019.5
72	5001.0
73	3428.5
74	2387.0
75	1641.0
76	1122.5
77	757.0
78	510.0
79	365.5
80	261.5
81	173.5
82	116.5
83	80.5
84	63.0
85	54.0
86	43.5
87	36.0
88	26.0
89	17.5
90	12.5
91	9.0
92	7.0
93	6.0
94	5.5
95	5.0
96	5.0
97	4.5
98	3.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0010067184071988996
3	0.002259980097793448
4	3.8008756190162536E-4
5	3.903601987097774E-4
6	5.64995024448362E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0010169910440070516
14-15	8.218109446521629E-4
16-17	7.704477606114027E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.140262074205749E-5
28-29	2.0059737899464605E-4
30-31	4.120095001150536E-5
32-33	0.0012440786887513865
34-35	0.00202951484085316
36-37	0.001315345780490313
38-39	7.689016423111405E-4
40-41	4.7396926562096056E-5
42-43	4.2428744371164114E-5
44-45	7.11300810342763E-4
46-47	1.6201100151906916E-5
48-49	0.0
50-51	0.0
52-53	0.0010262970022530272
54-55	0.0036978758248822513
56-57	0.006604875143020951
58-59	0.004695824010546
60-61	2.646759201587826E-4
62-63	1.7423194348705605E-5
64-65	1.055007010521585E-4
66-67	2.2490844450715578E-4
68-69	5.980010022496798E-6
70-71	1.2693235623626223E-4
72-73	1.8327291334471785E-5
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	2.6427602573784214E-5
88-89	3.077570293043567E-4
90-91	1.1530332132573588E-4
92-93	5.23108813653911E-4
94-95	3.357256584209647E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	243.0
20-21	965.0
22-23	1737.0
24-25	3473.0
26-27	5321.0
28-29	9331.0
30-31	21693.0
32-33	26464.0
34-35	35583.0
36-37	54484.0
38-39	65168.0
40-41	65216.0
42-43	74506.0
44-45	88968.0
46-47	89360.0
48-49	83383.0
50-51	77469.0
52-53	74185.0
54-55	79429.0
56-57	82960.0
58-59	84007.0
60-61	81711.0
62-63	78284.0
64-65	81389.0
66-67	86099.0
68-69	88642.0
70-71	88822.0
72-73	84314.0
74-75	85225.0
76-77	84025.0
78-79	90879.0
80-81	93463.0
82-83	89485.0
84-85	87848.0
86-87	92667.0
88-89	99771.0
90-91	106624.0
92-93	111762.0
94-95	131272.0
96-97	197039.0
98-99	825885.0
100-101	6025448.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.72738838964618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.99122919521182	77.14713056879589
2	7.052898791329929	12.092531879144138
3	1.6984070121978347	4.3679999273514705
4	0.6007843639240449	2.0601469801817247
5	0.2691998489441774	1.1538900002435788
6	0.12735533137479868	0.6550703973755673
7	0.0804867106572825	0.4829940853305352
8	0.04983122375572354	0.3417520538270619
9	0.029124526693875233	0.22470926503954136
>10	0.09839854092188949	1.275715983803754
>50	0.001854891444932817	0.10651237030756919
>100	3.733335344006879E-4	0.05381862224753176
>500	3.748713862587616E-5	0.020583552038253752
>1k	1.874287061744392E-5	0.017144314313387216
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.081791042445611E-5	0.0	1.0272636808152035E-5	1.0272636808152035E-5
3	0.0	3.081791042445611E-5	0.0	3.081791042445611E-5	1.0272636808152035E-5
4	0.0	8.218109446521628E-5	0.0	6.163582084891221E-5	1.0272636808152035E-5
5	0.0	9.245373127336832E-5	0.0	6.163582084891221E-5	1.0272636808152035E-5
6	0.0	9.245373127336832E-5	0.0	6.163582084891221E-5	2.054527361630407E-5
7	0.0	1.0272636808152036E-4	0.0	7.190845765706425E-5	2.054527361630407E-5
8	0.0	1.129990048896724E-4	0.0	7.190845765706425E-5	2.054527361630407E-5
9	0.0	1.5408955212228053E-4	0.0	1.129990048896724E-4	3.081791042445611E-5
10-11	0.0	1.5408955212228053E-4	0.0	1.2327164169782443E-4	3.081791042445611E-5
12-13	0.0	1.5408955212228053E-4	0.0	1.3354427850597645E-4	3.081791042445611E-5
14-15	0.0	2.3113432818342081E-4	0.0	1.4381691531412848E-4	3.081791042445611E-5
16-17	0.0	2.3627064658749683E-4	0.0	2.1058905456711673E-4	4.109054723260814E-5
18-19	0.0	2.5681592020380093E-4	0.0	2.77361193820105E-4	4.109054723260814E-5
20-21	0.0	2.9277014903233305E-4	0.0	3.544059698812452E-4	4.622686563668416E-5
22-23	0.0	3.8008756190162536E-4	0.0	4.4685970115461356E-4	6.163582084891221E-5
24-25	0.0	4.879502483872217E-4	0.0	5.393134324279819E-4	7.190845765706425E-5
26-27	0.0	5.239044772157538E-4	0.0	6.214945268931981E-4	7.190845765706425E-5
28-29	0.0	5.239044772157538E-4	0.0	0.0010580815912396597	7.190845765706425E-5
30-31	0.0	6.779940293380343E-4	0.0	0.002023709451205951	7.190845765706425E-5
32-33	0.0	7.088119397624904E-4	0.0	0.004216917409746411	9.245373127336832E-5
34-35	0.0	7.961293526317827E-4	0.0	0.0069494388007148526	9.245373127336832E-5
36-37	0.0	8.783104470969991E-4	0.0	0.010775996011751485	9.759004967744433E-5
38-39	0.0	9.091283575214552E-4	0.0	0.01663653531080222	1.129990048896724E-4
40-41	0.0	9.964457703907474E-4	0.0	0.0230877512263217	1.2327164169782443E-4
42-43	0.0	0.0010169910440070516	0.0	0.02909724375909064	1.2327164169782443E-4
44-45	0.0	0.001124853730492648	0.0	0.03529164375440632	1.2840796010190044E-4
46-47	0.0	0.0012121711433619403	0.0	0.04211267459501927	1.4895323371820452E-4
48-49	0.0	0.0012173074617660164	0.0	0.048815570112338474	1.5408955212228053E-4
50-51	0.0	0.0013354427850597646	0.0	0.05596532533081229	1.5408955212228053E-4
52-53	0.0	0.0013457154218679166	0.0	0.062303542241442106	2.054527361630407E-4
54-55	0.0	0.0013508517402719927	0.0	0.06909889149003468	2.568159202038009E-4
56-57	0.0	0.0013765333322923728	0.0	0.07645409944467152	2.568159202038009E-4
58-59	0.0	0.0013816696506964488	0.0	0.08319294919081927	2.568159202038009E-4
60-61	0.0	0.001391942287504601	0.0	0.09011157008110966	2.568159202038009E-4
62-63	0.0	0.001412487561120905	0.0	0.09604401783781746	2.670885570119529E-4
64-65	0.0	0.001489532337182045	0.0	0.10199187454973749	2.670885570119529E-4
66-67	0.0	0.0014946686555861212	0.0	0.10808354817697165	2.7222487541602895E-4
68-69	0.0	0.0015665771132431856	0.0	0.11438581085877292	2.77361193820105E-4
70-71	0.0	0.0016076676604757935	0.0	0.12028230438665219	2.77361193820105E-4
72-73	0.0	0.0016230766156880216	0.0	0.12460194816448011	3.0817910424456107E-4
74-75	0.0	0.0016693034813247058	0.0	0.12823332527616188	3.0817910424456107E-4
76-77	0.0	0.001730939302173618	0.0	0.13153084169157866	3.0817910424456107E-4
78-79	0.0	0.0017566208941939981	0.0	0.1346074964156202	3.0817910424456107E-4
80-81	0.0	0.0017566208941939981	0.0	0.13600971133993295	3.184517410527131E-4
82-83	0.0	0.0017617572125980742	0.0	0.13668256905086693	3.389970146690172E-4
84-85	0.0	0.00176689353100215	0.0	0.13725783671212344	3.389970146690172E-4
86-87	0.0	0.0017874388046184542	0.0	0.1375146526323272	3.389970146690172E-4
88-89	0.0	0.0018798925358918226	0.0	0.13756087949796392	3.389970146690172E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13325	0.0	35.230618	1
GTATCAA	21110	0.0	31.613297	1
TCAACGC	23230	0.0	27.70122	4
ATCAACG	23260	0.0	27.648046	3
CAACGCA	23620	0.0	27.225534	5
AACGCAG	24555	0.0	26.345919	6
TATCAAC	26045	0.0	25.231022	2
ACGCAGA	28470	0.0	22.633314	7
CGCAGAG	28895	0.0	22.27095	8
GTACATG	26855	0.0	21.680677	1
TACATGG	26125	0.0	21.553383	2
ACATGGG	26790	0.0	20.605204	3
TATACCG	885	0.0	18.754831	5
CATGGGG	18380	0.0	18.524256	4
GCAGAGT	34635	0.0	18.445326	9
AGAGTAC	28935	0.0	17.952923	10-11
GAGTACT	18485	0.0	17.821056	12-13
CAGAGTA	32365	0.0	16.780153	10-11
AGTACTT	20740	0.0	15.934728	12-13
GTACTTT	21200	0.0	15.549302	14-15
>>END_MODULE