FastQCFastQC Report
Fri 27 May 2016
SRR2138811_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138811_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10020415
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107250.045.298451
GTATCAA192650.035.837351
TCAACGC224400.029.7470654
ATCAACG225250.029.6351073
CAACGCA230900.028.9278625
TATCAAC243200.027.9356042
AACGCAG240050.027.9312236
ACGCAGA273200.024.4642947
CGCAGAG278050.023.9918698
GTACATG269050.021.3473471
TACATGG268000.020.9094352
ACATGGG269300.020.1320843
CTTATAC69150.019.8458671
GCAGAGT336000.019.8036619
GAGTACT190850.018.47559712-13
CATGGGG190550.018.338164
GTGGTAT59350.018.1985451
TGGTATC55450.018.1035632
AGAGTAC300100.018.07266210-11
CAGAGTA326800.017.40614310-11
Produced by FastQC (version 0.11.5)