FastQCFastQC Report
Fri 27 May 2016
SRR2138810_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138810_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8177931
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87950.044.8833271
GTATCAA154000.036.7838331
TCAACGC182950.030.1516324
ATCAACG182600.030.1401313
CAACGCA186500.029.532055
TATCAAC194550.028.7464872
AACGCAG192200.028.7444696
ACGCAGA223200.024.7713327
CGCAGAG226250.024.4186558
GTACATG231450.022.0200611
TACATGG227800.021.4794582
ACATGGG226750.021.0927413
GCAGAGT274150.020.0757129
CATGGGG166150.018.4758384
GTGGTAT48050.018.1779561
GAGTACT158100.018.02273212-13
AGAGTAC247050.017.85836610-11
CAGAGTA269950.017.32513810-11
GTACTTT178150.015.89919514-15
AGTACTT172850.015.73658512-13
Produced by FastQC (version 0.11.5)