##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138810_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8177931
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.471289376249324	34.0	31.0	34.0	31.0	34.0
2	32.66359315088376	34.0	31.0	34.0	31.0	34.0
3	32.7433706642915	34.0	31.0	34.0	31.0	34.0
4	36.19702831926559	37.0	37.0	37.0	35.0	37.0
5	36.01668111408619	37.0	35.0	37.0	35.0	37.0
6	36.086334673158774	37.0	35.0	37.0	35.0	37.0
7	36.08861226635441	37.0	35.0	37.0	35.0	37.0
8	36.07841396558616	37.0	35.0	37.0	35.0	37.0
9	37.941082652812796	39.0	38.0	39.0	35.0	39.0
10-11	37.816461889932796	39.0	38.0	39.0	35.0	39.0
12-13	37.82285971843001	39.0	38.0	39.0	35.0	39.0
14-15	39.156608132301436	40.0	39.0	41.0	36.0	41.0
16-17	39.13107679680839	40.0	39.0	41.0	36.0	41.0
18-19	39.20020503963656	40.0	39.0	41.0	36.0	41.0
20-21	39.22126707285928	40.0	39.0	41.0	36.0	41.0
22-23	39.18052226399013	40.0	39.0	41.0	36.0	41.0
24-25	39.1506944500609	40.0	39.0	41.0	36.0	41.0
26-27	39.06413073137864	40.0	39.0	41.0	36.0	41.0
28-29	39.00281571959674	40.0	39.0	41.0	36.0	41.0
30-31	38.87198653911659	40.0	38.0	41.0	35.0	41.0
32-33	38.73862542981366	40.0	38.0	41.0	35.0	41.0
34-35	38.70422737040663	40.0	38.0	41.0	35.0	41.0
36-37	38.65755447702118	40.0	38.0	41.0	35.0	41.0
38-39	38.589733950122174	40.0	38.0	41.0	35.0	41.0
40-41	38.502108204655315	40.0	38.0	41.0	34.0	41.0
42-43	38.52442859833587	40.0	38.0	41.0	34.0	41.0
44-45	38.46651792649406	40.0	38.0	41.0	34.0	41.0
46-47	38.41791348562991	40.0	38.0	41.0	34.0	41.0
48-49	38.376044627661535	40.0	38.0	41.0	34.0	41.0
50-51	38.316883138261616	40.0	38.0	41.0	34.0	41.0
52-53	38.06924346344174	40.0	37.0	41.0	33.0	41.0
54-55	37.92491175703829	40.0	37.0	41.0	33.0	41.0
56-57	37.83293956105892	39.5	37.0	41.0	33.0	41.0
58-59	37.70137231206229	39.0	36.5	41.0	33.0	41.0
60-61	37.519492999501324	39.0	36.0	41.0	33.0	41.0
62-63	37.271416473565225	39.0	35.5	40.0	32.5	41.0
64-65	37.03424241933433	38.5	35.0	40.0	32.0	41.0
66-67	36.77340766428415	38.0	35.0	40.0	32.0	41.0
68-69	36.52992525423014	37.5	35.0	40.0	31.5	41.0
70-71	36.22042667064966	37.0	35.0	39.5	31.0	41.0
72-73	35.929165662707604	36.5	35.0	39.0	31.0	41.0
74-75	35.476644874732386	36.0	34.0	39.0	31.0	40.0
76-77	33.54386240257662	34.5	32.0	36.5	28.0	39.0
78-79	34.73525823551064	35.0	34.0	37.0	30.0	39.0
80-81	34.70604977314133	35.0	34.0	37.0	31.0	39.0
82-83	34.43225412888747	35.0	34.0	36.5	30.5	39.0
84-85	34.15084590425011	35.0	34.0	36.0	30.0	37.0
86-87	33.921001622471216	35.0	34.0	36.0	30.0	37.0
88-89	33.780310925006816	35.0	34.0	35.0	30.0	36.5
90-91	33.61499680368367	35.0	34.0	35.0	30.0	36.0
92-93	33.57450073401141	35.0	34.0	35.0	30.0	36.0
94-95	33.44562923295082	35.0	33.0	35.0	30.0	36.0
96-97	33.46433967141455	35.0	33.0	35.0	30.0	35.5
98-99	33.558618714209125	35.0	33.0	35.0	30.0	35.0
100-101	32.279515480447095	34.0	31.5	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	10.0
20	31.0
21	130.0
22	533.0
23	2080.0
24	5360.0
25	11987.0
26	22437.0
27	36840.0
28	55386.0
29	78098.0
30	104276.0
31	137822.0
32	182741.0
33	252922.0
34	375372.0
35	589301.0
36	1069122.0
37	1992048.0
38	2328454.0
39	918417.0
40	14563.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.74067988590268	20.24245496813314	13.966491035446497	24.050374110517687
2	14.504623943551056	21.161517891949345	41.96899485293722	22.364863311562377
3	18.156251027941543	26.30603450753147	30.39246364923559	25.14525081529139
4	12.403188930965639	18.087120760516772	39.23906991918354	30.270620389334052
5	13.097816616847469	37.98850683640859	35.85233626460567	13.061340282138273
6	29.726878271319794	38.31328415684629	19.009935567747778	12.94990200408614
7	26.33985295302687	32.657991367254134	23.543754526664507	17.458401153054485
8	24.017725265718187	37.381961280915675	21.388710665325984	17.211602788040153
9	25.56982933702913	16.79862547140591	21.373975887055053	36.25756930450991
10-11	23.998790158537656	26.889246191976916	29.333446320346795	19.77851732913863
12-13	24.836454489360644	24.43256451637754	29.313580862246614	21.417400132015203
14-15	21.943626298450326	25.855248311189943	26.871667799703676	25.329457590656055
16-17	21.616539215773788	28.92018643858262	27.532828942710502	21.930445402933092
18-19	21.60086090234804	28.277042200527248	29.061360385652556	21.060736511472157
20-21	22.428232623436315	27.476792494602847	28.876118233165766	21.21885664879507
22-23	22.122520374548536	27.424605212357456	28.71112368840812	21.74175072468589
24-25	21.844303604625612	27.527889335936973	28.808871991209323	21.81893506822809
26-27	21.83531307283905	27.718957235705677	28.68022715986724	21.765502531588034
28-29	21.77174234028372	27.82870678944479	28.680044163688713	21.71950670658278
30-31	22.08641800875269	27.63734729611566	28.579467317484013	21.696767377647635
32-33	21.881831687378543	27.811484387558817	28.530208010209524	21.77647591485312
34-35	21.976460920133324	27.833468089333337	28.531070016061616	21.659000974471727
36-37	21.96977074810251	27.755727149336757	28.52326815098913	21.751233951571606
38-39	21.84608620532374	27.882020377145178	28.56684783340171	21.705045584129376
40-41	22.105985113471558	27.722839139986537	28.43001074728123	21.741164999260672
42-43	21.92103390880343	27.846804007576488	28.27612397254193	21.956038111078147
44-45	22.06824365448686	27.942693503328258	28.10544632183925	21.883616520345633
46-47	22.065683951564345	27.882994419836184	27.90960897610387	22.141712652495606
48-49	22.05633296415537	27.991220177424115	27.89695352679508	22.05549333162543
50-51	22.059350660476674	28.109513302824862	27.75427024856502	22.076865788133443
52-53	22.229657559637435	28.014595262510483	27.63706302316578	22.118684154686296
54-55	22.16166310224203	27.904678514568147	27.73278794695024	22.200870436239583
56-57	22.181270310487065	27.987792974252596	27.707160135628524	22.12377657963182
58-59	22.139586650582153	28.087821488086252	27.67665525378623	22.095936607545365
60-61	22.250038912937	28.043621299159494	27.64188565142734	22.06445413647617
62-63	22.20934958925925	28.027874646772684	27.636109333539	22.126666430429072
64-65	22.161252072140222	28.045905832520564	27.625578431559607	22.167263663779607
66-67	22.248874179018006	28.05371855382456	27.448816527460167	22.248590739697264
68-69	22.18025341383724	28.04569559527814	27.55141177037665	22.22263922050797
70-71	22.193477113108884	27.838301737086912	27.60655545867377	22.36166569113043
72-73	22.233520026519297	27.907136782872094	27.58754208064021	22.271801109968397
74-75	22.192777055996274	27.979267748246194	27.53630759934173	22.2916475964158
76-77	22.182867701204124	27.958875157283213	27.503740420957552	22.35451672055511
78-79	22.184770771780208	27.96686788573218	27.494245795411693	22.354115547075917
80-81	22.18900908024368	27.958957151576158	27.50839013780987	22.343643630370295
82-83	22.23667383331206	27.9475919583217	27.573387200589888	22.242347007776356
84-85	22.370651096889112	27.938191354481866	27.46457907500827	22.22657847362075
86-87	22.16213838888565	27.959911638764943	27.556568608008874	22.321381364340528
88-89	22.225290978703114	27.91947050385264	27.517316848052157	22.337921669392085
90-91	22.24561221469892	27.94097205567525	27.545272768860652	22.26814296076518
92-93	22.303201878697852	27.90473813387958	27.517817337079293	22.27424265034328
94-95	22.116155388444596	27.968807226237256	27.586111400815376	22.328925984502774
96-97	22.13281593984983	27.9751384612003	27.606644145613856	22.285401453336018
98-99	22.366700567263223	28.572799794181126	27.65156174284431	21.40893789571134
100-101	23.14397449579619	31.155961041087956	24.75241503159241	20.94764943152345
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1819.0
1	1580.0
2	2030.5
3	2948.5
4	4154.5
5	6650.0
6	8635.5
7	9951.5
8	10746.5
9	10474.0
10	9521.5
11	8655.0
12	9373.5
13	12357.0
14	19322.0
15	28547.0
16	35700.5
17	39519.0
18	40245.0
19	38839.5
20	36611.0
21	34748.5
22	35295.0
23	39898.5
24	48370.5
25	60716.0
26	76952.5
27	95084.0
28	113594.5
29	132509.5
30	150015.5
31	166518.0
32	184261.5
33	201905.0
34	218393.5
35	235096.5
36	253528.0
37	270331.0
38	283312.5
39	293979.5
40	304885.5
41	315679.5
42	322871.0
43	328550.0
44	335019.0
45	341098.5
46	344639.0
47	347196.0
48	347735.0
49	343262.5
50	334931.5
51	324059.0
52	309622.5
53	292154.0
54	273720.5
55	252050.0
56	229614.5
57	206832.5
58	182607.5
59	157693.0
60	131673.5
61	107468.0
62	85891.5
63	67113.0
64	50811.5
65	37775.0
66	28298.5
67	21472.0
68	16520.0
69	12185.5
70	8750.5
71	6330.5
72	4542.5
73	3173.5
74	2208.5
75	1550.0
76	1043.5
77	693.0
78	482.5
79	346.5
80	245.0
81	163.5
82	117.0
83	88.0
84	65.0
85	53.5
86	45.5
87	32.0
88	18.5
89	10.0
90	6.0
91	4.0
92	3.5
93	3.0
94	2.5
95	2.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.703660008870214E-4
3	0.002201045716820061
4	2.2010457168200614E-4
5	3.179288257628977E-4
6	4.768932386443466E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0010393826996094734
14-15	7.031118262064085E-4
16-17	1.2228031760111452E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.894260421272242E-5
28-29	2.326203519545925E-4
30-31	3.6778563197616554E-5
32-33	0.001345733664805765
34-35	0.0020587441056892654
36-37	0.0013744263704625124
38-39	7.975725878289426E-4
40-41	5.648532846940262E-5
42-43	4.426107432629429E-5
44-45	6.953291994630272E-4
46-47	1.288840331113392E-5
48-49	0.0
50-51	0.0
52-53	0.001139807004167055
54-55	0.003796917330948941
56-57	0.006288824805554193
58-59	0.004701391461973306
60-61	2.408398788451549E-4
62-63	2.7792650705995865E-5
64-65	1.262643090781934E-4
66-67	2.409228421922788E-4
68-69	0.0
70-71	9.413801153871332E-5
72-73	1.464183509047556E-5
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	1.5877456520379192E-5
88-89	3.8605057712952736E-4
90-91	1.2236088973986809E-4
92-93	4.7202408482189997E-4
94-95	2.356910491021055E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	147.0
20-21	549.0
22-23	1163.0
24-25	2438.0
26-27	4113.0
28-29	8120.0
30-31	19242.0
32-33	23452.0
34-35	31477.0
36-37	48393.0
38-39	58051.0
40-41	57835.0
42-43	66174.0
44-45	77848.0
46-47	78665.0
48-49	72610.0
50-51	66635.0
52-53	64259.0
54-55	69096.0
56-57	71484.0
58-59	71755.0
60-61	71119.0
62-63	68275.0
64-65	70348.0
66-67	74879.0
68-69	75994.0
70-71	75593.0
72-73	73271.0
74-75	73825.0
76-77	72585.0
78-79	77552.0
80-81	80206.0
82-83	76502.0
84-85	76418.0
86-87	79966.0
88-89	85839.0
90-91	91231.0
92-93	94398.0
94-95	111310.0
96-97	165767.0
98-99	689857.0
100-101	4999490.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.69744308430124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.87437630904886	77.02004248481786
2	7.140392916989382	12.238268312064905
3	1.7187896916898042	4.41887645332412
4	0.6296371424047625	2.1583317269997684
5	0.2549563737572212	1.0924554664519648
6	0.1348755508026438	0.6935093903024008
7	0.07621819162431143	0.4572192897098955
8	0.047043002251961105	0.3225172006401673
9	0.0265175346339633	0.20452364235270512
>10	0.0951451760750105	1.2234584343473596
>50	0.0016247424034865512	0.09393138672169485
>100	4.014638909533284E-4	0.06473980102051297
>500	2.1904427672429696E-5	0.01212641124663945
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2228031760111451E-5
2	0.0	4.8912127040445806E-5	0.0	0.0	1.2228031760111451E-5
3	0.0	4.8912127040445806E-5	0.0	0.0	1.2228031760111451E-5
4	0.0	6.114015880055726E-5	0.0	0.0	1.2228031760111451E-5
5	0.0	6.114015880055726E-5	0.0	0.0	1.2228031760111451E-5
6	7.33681905606687E-5	6.114015880055726E-5	0.0	0.0	1.2228031760111451E-5
7	7.33681905606687E-5	6.114015880055726E-5	0.0	0.0	2.4456063520222903E-5
8	7.33681905606687E-5	6.114015880055726E-5	0.0	0.0	4.8912127040445806E-5
9	7.33681905606687E-5	1.3450834936122596E-4	0.0	3.668409528033435E-5	4.8912127040445806E-5
10-11	7.33681905606687E-5	1.3450834936122596E-4	0.0	3.668409528033435E-5	4.8912127040445806E-5
12-13	7.33681905606687E-5	1.3450834936122596E-4	0.0	4.8912127040445806E-5	5.5026142920501536E-5
14-15	7.33681905606687E-5	2.0787653992189467E-4	0.0	8.559622232078016E-5	9.171023820083587E-5
16-17	9.782425408089161E-5	2.323326034421176E-4	0.0	1.2228031760111452E-4	1.1005228584100306E-4
18-19	9.782425408089161E-5	2.812447304825634E-4	0.0	1.2228031760111452E-4	1.467363811213374E-4
20-21	9.782425408089161E-5	3.179288257628977E-4	0.0	1.3450834936122596E-4	1.5896441288144886E-4
22-23	1.0393826996094733E-4	3.3015685752300916E-4	0.0	1.711924446415603E-4	1.711924446415603E-4
24-25	1.1005228584100306E-4	3.729549686833993E-4	0.0	2.2010457168200612E-4	1.8342047640167178E-4
26-27	1.1005228584100306E-4	4.035250480836779E-4	0.0	2.6901669872245193E-4	1.895344922817275E-4
28-29	1.1005228584100306E-4	4.096390639637336E-4	0.0	5.991735562454611E-4	2.0176252404183895E-4
30-31	1.1005228584100306E-4	5.13577333924681E-4	0.0	0.0016752403511352688	2.0787653992189467E-4
32-33	1.1005228584100306E-4	5.196913498047367E-4	0.0	0.0036011553533528227	2.139905558019504E-4
34-35	1.1005228584100306E-4	5.930595403654054E-4	0.0	0.006230182181776784	2.3233260344211756E-4
36-37	1.1005228584100306E-4	6.603137150460183E-4	0.0	0.010033100059171445	2.3233260344211756E-4
38-39	1.1005228584100306E-4	6.847697785662413E-4	0.0	0.016128773891587005	2.3233260344211756E-4
40-41	1.1005228584100306E-4	7.459099373667986E-4	0.0	0.022548490565645514	2.4456063520222903E-4
42-43	1.1005228584100306E-4	7.825940326471329E-4	0.0	0.027696491936652436	2.4456063520222903E-4
44-45	1.1005228584100306E-4	8.987603343681917E-4	0.0	0.03360263127678627	2.4456063520222903E-4
46-47	1.1005228584100306E-4	9.782425408089161E-4	0.0	0.040456443078328734	2.629026828423962E-4
48-49	1.1005228584100306E-4	9.843565566889719E-4	0.0	0.04756704354683355	2.934727622426748E-4
50-51	1.1005228584100306E-4	0.0011494349854504764	0.0	0.05402344431617239	2.9958677812273054E-4
52-53	1.1005228584100306E-4	0.0011616630172105878	0.0	0.06092005422887525	3.362708734030649E-4
54-55	1.1005228584100306E-4	0.0011738910489706993	0.0	0.06790226036389889	3.6684095280334355E-4
56-57	1.1005228584100306E-4	0.0012105751442510336	0.0	0.07426083687915684	3.6684095280334355E-4
58-59	1.1005228584100306E-4	0.0012166891601310893	0.0	0.08047267701329346	3.6684095280334355E-4
60-61	1.2839433348117024E-4	0.0012289171918912008	0.0	0.08670897321095031	3.729549686833993E-4
62-63	1.467363811213374E-4	0.0012533732554114237	0.0	0.09279241901160576	3.79068984563455E-4
64-65	1.467363811213374E-4	0.0013695395571324826	0.0	0.09888809284402131	3.79068984563455E-4
66-67	1.467363811213374E-4	0.0013756535730125384	0.0	0.10503879281935737	3.851830004435107E-4
68-69	1.467363811213374E-4	0.0014123376682928727	0.0	0.11109166854061253	3.9129701632356645E-4
70-71	1.467363811213374E-4	0.0014306797159330399	0.0	0.11715677229362781	4.035250480836779E-4
72-73	1.467363811213374E-4	0.0014306797159330399	0.0	0.12202352893415216	4.4020914336401223E-4
74-75	1.467363811213374E-4	0.0014551357794532628	0.0	0.12617494571671	4.4020914336401223E-4
76-77	1.5285039700139313E-4	0.0014979338906136528	0.0	0.1300695738323055	4.4020914336401223E-4
78-79	1.5896441288144886E-4	0.00151627593825382	0.0	0.13385414966206	4.4020914336401223E-4
80-81	1.5896441288144886E-4	0.0015223899541338757	0.0	0.13540710969559416	4.4632315924406795E-4
82-83	1.5896441288144886E-4	0.0015285039700139314	0.0	0.13597571317243934	4.7689323864434657E-4
84-85	1.5896441288144886E-4	0.0015407320017740429	0.0	0.13652597460164434	4.7689323864434657E-4
86-87	1.5896441288144886E-4	0.0015529600335341543	0.0	0.1367399651574463	4.7689323864434657E-4
88-89	1.5896441288144886E-4	0.001626328224094823	0.0	0.13680721933212692	4.7689323864434657E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8795	0.0	44.883327	1
GTATCAA	15400	0.0	36.783833	1
TCAACGC	18295	0.0	30.151632	4
ATCAACG	18260	0.0	30.140131	3
CAACGCA	18650	0.0	29.53205	5
TATCAAC	19455	0.0	28.746487	2
AACGCAG	19220	0.0	28.744469	6
ACGCAGA	22320	0.0	24.771332	7
CGCAGAG	22625	0.0	24.418655	8
GTACATG	23145	0.0	22.020061	1
TACATGG	22780	0.0	21.479458	2
ACATGGG	22675	0.0	21.092741	3
GCAGAGT	27415	0.0	20.075712	9
CATGGGG	16615	0.0	18.475838	4
GTGGTAT	4805	0.0	18.177956	1
GAGTACT	15810	0.0	18.022732	12-13
AGAGTAC	24705	0.0	17.858366	10-11
CAGAGTA	26995	0.0	17.325138	10-11
GTACTTT	17815	0.0	15.899195	14-15
AGTACTT	17285	0.0	15.736585	12-13
>>END_MODULE