FastQCFastQC Report
Fri 27 May 2016
SRR2138809_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138809_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11538095
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA138350.048.432141
GTATCAA253900.037.0522081
ATCAACG304900.029.9636123
TCAACGC305650.029.9576444
CAACGCA313300.029.1856485
TATCAAC323750.028.5290582
AACGCAG322650.028.405116
ACGCAGA375050.024.3584297
CGCAGAG378950.024.0967168
GTACATG392400.023.037571
TACATGG387200.022.8722532
ACATGGG389850.022.0307043
GCAGAGT454200.020.0309819
CATGGGG256700.019.739064
GAGTACT241650.018.35808212-13
AGAGTAC404350.017.95497310-11
GTGGTAT68100.017.8506621
CAGAGTA435350.017.82307810-11
GTACTTT275250.016.02631814-15
TGGTATC67350.015.8165232
Produced by FastQC (version 0.11.5)