##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138809_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11538095
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45782557692583	33.0	31.0	34.0	31.0	34.0
2	32.64196056628066	34.0	31.0	34.0	31.0	34.0
3	32.72203609001313	34.0	31.0	34.0	31.0	34.0
4	36.185577948526166	37.0	35.0	37.0	35.0	37.0
5	36.00677338850131	37.0	35.0	37.0	35.0	37.0
6	36.07525341055001	37.0	35.0	37.0	35.0	37.0
7	36.0759126181575	37.0	35.0	37.0	35.0	37.0
8	36.06583868480889	37.0	35.0	37.0	35.0	37.0
9	37.92625255728957	39.0	38.0	39.0	35.0	39.0
10-11	37.80336177679244	39.0	38.0	39.0	35.0	39.0
12-13	37.8069182131019	39.0	38.0	39.0	35.0	39.0
14-15	39.1251999571853	40.0	38.5	41.0	36.0	41.0
16-17	39.102147841563095	40.0	38.0	41.0	36.0	41.0
18-19	39.18006633677396	40.0	39.0	41.0	36.0	41.0
20-21	39.200652358827014	40.0	39.0	41.0	36.0	41.0
22-23	39.16062076725848	40.0	39.0	41.0	36.0	41.0
24-25	39.12821364796909	40.0	39.0	41.0	36.0	41.0
26-27	39.03981868122463	40.0	39.0	41.0	36.0	41.0
28-29	38.977072924487906	40.0	38.5	41.0	35.0	41.0
30-31	38.842223560481926	40.0	38.0	41.0	35.0	41.0
32-33	38.70774980251098	40.0	38.0	41.0	35.0	41.0
34-35	38.666807322290424	40.0	38.0	41.0	35.0	41.0
36-37	38.619438638736355	40.0	38.0	41.0	35.0	41.0
38-39	38.55055088550782	40.0	38.0	41.0	35.0	41.0
40-41	38.46978919717047	40.0	38.0	41.0	34.0	41.0
42-43	38.489360550668465	40.0	38.0	41.0	34.0	41.0
44-45	38.429465084630074	40.0	38.0	41.0	34.0	41.0
46-47	38.38296903272988	40.0	38.0	41.0	34.0	41.0
48-49	38.33753914421527	40.0	38.0	41.0	34.0	41.0
50-51	38.27635875139761	40.0	38.0	41.0	34.0	41.0
52-53	38.02767484181914	40.0	37.0	41.0	33.0	41.0
54-55	37.88093556545197	40.0	37.0	41.0	33.0	41.0
56-57	37.78289615779306	39.0	37.0	41.0	33.0	41.0
58-59	37.64882168414519	39.0	36.0	41.0	33.0	41.0
60-61	37.4641600661858	39.0	36.0	41.0	33.0	41.0
62-63	37.21367264215978	39.0	35.0	40.0	32.0	41.0
64-65	36.97148008696263	38.5	35.0	40.0	32.0	41.0
66-67	36.70671747864796	38.0	35.0	40.0	32.0	41.0
68-69	36.46540316751771	37.0	35.0	40.0	31.5	41.0
70-71	36.1566218134258	37.0	35.0	39.0	31.0	41.0
72-73	35.86506947252266	36.0	34.5	39.0	31.0	41.0
74-75	35.41722454828758	36.0	34.0	39.0	31.0	40.0
76-77	33.500752162264895	34.5	32.0	36.5	28.0	39.0
78-79	34.688678039913725	35.0	34.0	37.0	30.0	39.0
80-81	34.66430695519921	35.0	34.0	37.0	31.0	39.0
82-83	34.40121790764415	35.0	34.0	36.0	30.5	38.5
84-85	34.12871580085937	35.0	34.0	36.0	30.0	37.0
86-87	33.9056323389713	35.0	34.0	35.5	30.0	37.0
88-89	33.77447758988188	35.0	34.0	35.0	30.0	36.5
90-91	33.60879470576598	35.0	34.0	35.0	30.0	36.0
92-93	33.57524166313299	35.0	34.0	35.0	30.0	36.0
94-95	33.44999435249426	35.0	33.0	35.0	30.0	36.0
96-97	33.46933852385044	35.0	33.0	35.0	30.0	35.5
98-99	33.563191883818575	35.0	33.0	35.0	30.0	35.0
100-101	32.28238591362789	34.0	31.5	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	6.0
19	7.0
20	49.0
21	210.0
22	884.0
23	3006.0
24	7800.0
25	16936.0
26	31700.0
27	51787.0
28	77753.0
29	108773.0
30	146963.0
31	194369.0
32	258988.0
33	356954.0
34	531095.0
35	836682.0
36	1525188.0
37	2819713.0
38	3213187.0
39	1333127.0
40	22917.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.99188860899481	20.14111514942458	13.972713866543826	23.89428237503678
2	14.615857370045488	21.160889971616253	41.890836501937386	22.332416156400868
3	18.188426497798023	26.4297868121178	30.45359411548333	24.928192574600853
4	12.443432796159765	18.03588753242967	39.070671065100626	30.450008606309943
5	13.098176718214125	38.089316895303114	35.64410110867174	13.168405277811027
6	29.620762015567298	38.18373878664923	18.99314371809681	13.202355479686656
7	26.406560181728437	32.760694031380396	23.383894828392382	17.44885095849878
8	24.30119530130407	37.04889758664667	21.315719796032187	17.334187316017072
9	25.83509669490501	16.69458433129559	21.23222247693402	36.23809649686539
10-11	24.153086976363387	26.84330588556022	29.137878875927044	19.86572826214935
12-13	25.03491946941127	24.394924205077217	29.15408213710966	21.416074188401847
14-15	22.187199295198322	25.694970032711485	26.771170647246194	25.346660024844
16-17	21.67790033131142	28.788515808556483	27.44557655008148	22.088007310050617
18-19	21.736733836911554	28.038723030101593	29.069231099241254	21.155312033745606
20-21	22.672072391821036	27.218532528064898	28.827418302544235	21.281976777569838
22-23	22.319570590245984	27.16645288087282	28.696635336505572	21.81734119237562
24-25	22.000098139136327	27.34073977489032	28.835436967105355	21.823725118867994
26-27	21.957642292456185	27.520450890025437	28.722536366532953	21.79937045098542
28-29	21.89428044679924	27.564372781415038	28.77174033735183	21.769606434433893
30-31	22.2075618151047	27.45755775202378	28.589323509629327	21.745556923242187
32-33	22.070690352267444	27.58486619627144	28.522024106313914	21.8224193451472
34-35	22.060117269996457	27.578186556363494	28.58897784462819	21.772718329011862
36-37	22.07269954957629	27.536293335063945	28.558583928418347	21.832423186941416
38-39	21.91069834806273	27.61418072429489	28.655275411975335	21.819845515667048
40-41	22.16637276887588	27.51036791637248	28.50451366510963	21.81874564964201
42-43	22.081381011985115	27.575995534277236	28.341766557636344	22.000856896101304
44-45	22.115994927295784	27.75801019086277	28.161803809982683	21.964191071858764
46-47	22.207814582414077	27.658400409803686	27.909146692738396	22.224638315043848
48-49	22.159377213792755	27.747638547399774	27.882942908705544	22.210041330101934
50-51	22.12845026867137	27.856591275201215	27.787732053015535	22.227226403111885
52-53	22.31230772709747	27.803476451801153	27.628693269220356	22.255522551881022
54-55	22.330353934156786	27.661769004244878	27.66958608212711	22.33829097947122
56-57	22.306187407440863	27.811783270823952	27.608117391728094	22.27391193000709
58-59	22.22171963482654	27.91668949977896	27.643251430612025	22.218339434782475
60-61	22.374557857108865	27.85597598349237	27.556945691968483	22.21252046743028
62-63	22.31088231059526	27.911462671320404	27.48877811856194	22.28887689952239
64-65	22.273721987329708	27.86065236283399	27.522496451240446	22.34312919859586
66-67	22.40321667812238	27.844721729881567	27.380531695048184	22.371529896947873
68-69	22.33837021021698	27.826522520989904	27.475581167003487	22.35952610178963
70-71	22.359487727374276	27.572750632900927	27.549641278477516	22.51812036124728
72-73	22.42854848071439	27.621324074899945	27.48956392315985	22.46056352122581
74-75	22.278728277522262	27.779734792194482	27.45708557023499	22.48445136004826
76-77	22.37038666712351	27.68684171310376	27.432850986478474	22.509920633294257
78-79	22.350041766840295	27.731477186759214	27.37162148549142	22.54685956090907
80-81	22.375136169053583	27.6806275657504	27.407041037698665	22.537195227497353
82-83	22.460539401322308	27.602394810894154	27.497136587207887	22.439929200575655
84-85	22.55405462905083	27.582450541091134	27.41763075316883	22.4458640766892
86-87	22.353237437280587	27.60935464963788	27.488324750371813	22.54908316270972
88-89	22.454232084532848	27.583708523127815	27.42625188731761	22.535807505021726
90-91	22.497011039868873	27.523110205852575	27.52011521959215	22.4597635346864
92-93	22.52696081540987	27.46020792463763	27.499783489726383	22.51304777022612
94-95	22.35767723054728	27.500318418492576	27.563315668517518	22.57868868244263
96-97	22.373311682766513	27.51421455728712	27.618021292839636	22.494452467106733
98-99	22.632398422662202	28.108138281541773	27.6475298608333	21.611933434962726
100-101	23.45410653372611	30.67350277822435	24.652658714226156	21.219731973823382
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2027.0
1	1881.5
2	2876.0
3	4400.0
4	6366.5
5	10508.0
6	13818.0
7	16153.0
8	17776.5
9	17481.5
10	15795.0
11	14161.0
12	15479.0
13	20675.5
14	32184.0
15	47223.0
16	58350.5
17	63592.5
18	63621.5
19	59825.5
20	54998.5
21	51082.0
22	50810.0
23	57175.0
24	69755.0
25	88282.0
26	112435.0
27	137476.5
28	160878.0
29	184867.0
30	206408.5
31	227058.5
32	250451.5
33	275721.0
34	301477.5
35	326665.0
36	354862.0
37	377955.5
38	393579.0
39	407124.0
40	422494.5
41	438814.0
42	450258.5
43	462264.0
44	474235.0
45	482074.0
46	488599.0
47	495082.5
48	495559.0
49	489709.5
50	481787.0
51	468364.5
52	448898.0
53	428152.5
54	405186.5
55	377125.0
56	346011.5
57	312944.0
58	280203.5
59	245767.0
60	207194.5
61	169517.0
62	134732.0
63	104537.5
64	79195.0
65	58608.0
66	43335.5
67	32802.0
68	24910.5
69	17732.0
70	12463.5
71	8958.0
72	6294.0
73	4227.5
74	2854.5
75	1981.0
76	1383.0
77	948.5
78	639.5
79	434.5
80	302.0
81	204.0
82	149.5
83	117.5
84	91.0
85	69.5
86	51.5
87	40.5
88	28.5
89	18.0
90	14.0
91	10.5
92	8.0
93	4.5
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001334709065924661
3	0.0025220801180784176
4	4.246801573396648E-4
5	4.6801486727228366E-4
6	5.460173451509977E-4
7	0.0
8	0.0
9	0.0
10-11	4.333470993261886E-6
12-13	0.001031366096396329
14-15	8.450268436860679E-4
16-17	6.933553589219017E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.769834043164136E-5
28-29	1.95257007687225E-4
30-31	8.691081420831896E-6
32-33	0.0012505333284996807
34-35	0.0020287909927276587
36-37	0.0013489109850565268
38-39	7.124793862233481E-4
40-41	2.2306663352788758E-5
42-43	2.24977921791645E-5
44-45	6.318113180497721E-4
46-47	9.198322078879658E-6
48-49	0.0
50-51	0.0
52-53	0.0011116525798748507
54-55	0.00406433140836617
56-57	0.0066457937001077345
58-59	0.00495967017948021
60-61	2.0345193423242547E-4
62-63	1.5055011010231386E-5
64-65	7.611546838338508E-5
66-67	2.156606610307347E-4
68-69	0.0
70-71	1.2110150559710097E-4
72-73	2.1325584468960693E-5
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	3.507264626447967E-5
88-89	4.0333790588026927E-4
90-91	1.5667911622854565E-4
92-93	6.1903474219103E-4
94-95	3.1202106716322116E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	196.0
20-21	727.0
22-23	1565.0
24-25	3628.0
26-27	6055.0
28-29	12949.0
30-31	29532.0
32-33	36952.0
34-35	49661.0
36-37	75127.0
38-39	91753.0
40-41	93075.0
42-43	105968.0
44-45	127087.0
46-47	126317.0
48-49	118726.0
50-51	108340.0
52-53	103292.0
54-55	111268.0
56-57	114592.0
58-59	115952.0
60-61	114380.0
62-63	109547.0
64-65	113652.0
66-67	121286.0
68-69	120212.0
70-71	119162.0
72-73	114448.0
74-75	115248.0
76-77	113629.0
78-79	121628.0
80-81	123195.0
82-83	117656.0
84-85	117528.0
86-87	122335.0
88-89	130044.0
90-91	139135.0
92-93	140901.0
94-95	160600.0
96-97	229554.0
98-99	939175.0
100-101	6722018.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.77389013100945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.96032673016265	77.16247181102685
2	7.067814999051726	12.12467974389927
3	1.7305063875933069	4.452967942813151
4	0.6042324293169248	2.0730946402329136
5	0.2584494512219905	1.108410741676735
6	0.1356900338903865	0.6983197235272176
7	0.07828780345529597	0.4700534616520833
8	0.047239673575846054	0.32415444568954954
9	0.02799978592988166	0.21614855058373086
>10	0.08747012467627965	1.1195164093413574
>50	0.0014728113726999884	0.08301620245868846
>100	3.846093069156019E-4	0.05355938584630036
>500	7.822706793901171E-5	0.049384270545869795
>1k	4.693337820563782E-5	0.06422267070630874
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.666941986523772E-6
2	0.0	6.06685939056664E-5	0.0	8.666941986523772E-6	1.7333883973047543E-5
3	0.0	6.06685939056664E-5	0.0	1.7333883973047543E-5	1.7333883973047543E-5
4	0.0	1.3867107178438035E-4	0.0	1.7333883973047543E-5	2.6000825959571315E-5
5	0.0	1.3867107178438035E-4	0.0	1.7333883973047543E-5	2.6000825959571315E-5
6	1.7333883973047543E-5	1.3867107178438035E-4	0.0	2.6000825959571315E-5	6.06685939056664E-5
7	1.7333883973047543E-5	1.3867107178438035E-4	0.0	2.6000825959571315E-5	6.06685939056664E-5
8	1.7333883973047543E-5	1.560049557574279E-4	0.0	3.466776794609509E-5	6.06685939056664E-5
9	1.7333883973047543E-5	2.3400743363614183E-4	0.0	5.200165191914263E-5	1.3000412979785657E-4
10-11	1.7333883973047543E-5	2.3400743363614183E-4	0.0	9.533636185176148E-5	1.3867107178438035E-4
12-13	1.7333883973047543E-5	2.3400743363614183E-4	0.0	1.3433760079111846E-4	1.4733801377090413E-4
14-15	1.7333883973047543E-5	2.9467602754180825E-4	0.0	1.560049557574279E-4	1.9500619469678485E-4
16-17	1.7333883973047543E-5	2.9900949853507014E-4	0.0	1.863392527102611E-4	2.2534049164961808E-4
18-19	1.7333883973047543E-5	3.2934379548790337E-4	0.0	2.0800660767657052E-4	2.426743756226656E-4
20-21	1.7333883973047543E-5	3.640115634339984E-4	0.0	2.3834090462940372E-4	2.5567478860245125E-4
22-23	1.7333883973047543E-5	4.376805703194505E-4	0.0	2.730086725754988E-4	2.860090855552845E-4
24-25	2.166735496630943E-5	4.983491642251168E-4	0.0	3.856789184003078E-4	2.9900949853507014E-4
26-27	2.6000825959571315E-5	5.330169321712119E-4	0.0	6.500206489892828E-4	3.0334296952833203E-4
28-29	2.6000825959571315E-5	5.503508161442595E-4	0.0	0.0024830788791390607	3.0334296952833203E-4
30-31	2.6000825959571315E-5	6.500206489892828E-4	0.0	0.006383202773074758	3.0334296952833203E-4
32-33	2.6000825959571315E-5	6.500206489892828E-4	0.0	0.014850805093908483	3.2067685350137953E-4
34-35	2.6000825959571315E-5	7.713578368006156E-4	0.0	0.022529715693968545	3.2067685350137953E-4
36-37	2.6000825959571315E-5	8.926950246119485E-4	0.0	0.03329839111222434	3.2067685350137953E-4
38-39	2.6000825959571315E-5	9.273627925580435E-4	0.0	0.05014259286303328	3.250103244946414E-4
40-41	2.6000825959571315E-5	0.0010486999803693763	0.0	0.06615476818313595	3.5534462144747465E-4
42-43	2.6000825959571315E-5	0.0010747008063289477	0.0	0.07735245722972467	3.5967809244073654E-4
44-45	2.6000825959571315E-5	0.0011960379941402806	0.0	0.08977218509641323	3.640115634339984E-4
46-47	2.6000825959571315E-5	0.0012827074140055181	0.0	0.10245625469369077	3.640115634339984E-4
48-49	2.6000825959571315E-5	0.0012827074140055181	0.0	0.11382728258000996	3.986793313800935E-4
50-51	2.6000825959571315E-5	0.0014430458407562081	0.0	0.12435761709363635	4.1167974435987915E-4
52-53	2.6000825959571315E-5	0.0014560462537359937	0.0	0.13341457146955368	4.5068098329923616E-4
54-55	2.6000825959571315E-5	0.0014690466667157793	0.0	0.1428485378218848	4.896822222385932E-4
56-57	2.6000825959571315E-5	0.001482047079695565	0.0	0.15181015583595037	4.94015693231855E-4
58-59	2.6000825959571315E-5	0.0014907140216820888	0.0	0.16046409740949438	4.983491642251169E-4
60-61	2.6000825959571315E-5	0.0015123813766483984	0.0	0.1686456906447728	5.026826352183787E-4
62-63	2.6000825959571315E-5	0.0015427156736012315	0.0	0.17641560413569138	5.113495772049025E-4
64-65	2.6000825959571315E-5	0.0017247214553182306	0.0	0.18427652051746846	5.286834611779501E-4
66-67	3.466776794609509E-5	0.001750722281277802	0.0	0.19244077986877384	5.330169321712119E-4
68-69	3.466776794609509E-5	0.0018113908751834684	0.0	0.20025836154061827	5.416838741577357E-4
70-71	3.466776794609509E-5	0.0018417251721363015	0.0	0.2073262527306284	5.850185840903546E-4
72-73	4.766818092588074E-5	0.0018460586431295635	0.0	0.2126260877553877	6.586875909758066E-4
74-75	5.200165191914263E-5	0.0018937268240554442	0.0	0.2167558856119663	6.586875909758066E-4
76-77	5.200165191914263E-5	0.0019717293019341584	0.0	0.22068201033186152	6.760214749488542E-4
78-79	5.200165191914263E-5	0.0019933966569004673	0.0	0.22416178753945082	7.020223009084255E-4
80-81	5.200165191914263E-5	0.001997730127893729	0.0	0.22573050403901163	7.106892428949493E-4
82-83	5.200165191914263E-5	0.002015064011866777	0.0	0.2263501903910481	7.583574238208301E-4
84-85	5.200165191914263E-5	0.00204539830881961	0.0	0.22694387591712495	7.843582497804014E-4
86-87	5.200165191914263E-5	0.0020713991347791815	0.0	0.2271648829377813	7.886917207736632E-4
88-89	5.200165191914263E-5	0.0021970697935837762	0.0	0.2272472188866533	7.886917207736632E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13835	0.0	48.43214	1
GTATCAA	25390	0.0	37.052208	1
ATCAACG	30490	0.0	29.963612	3
TCAACGC	30565	0.0	29.957644	4
CAACGCA	31330	0.0	29.185648	5
TATCAAC	32375	0.0	28.529058	2
AACGCAG	32265	0.0	28.40511	6
ACGCAGA	37505	0.0	24.358429	7
CGCAGAG	37895	0.0	24.096716	8
GTACATG	39240	0.0	23.03757	1
TACATGG	38720	0.0	22.872253	2
ACATGGG	38985	0.0	22.030704	3
GCAGAGT	45420	0.0	20.030981	9
CATGGGG	25670	0.0	19.73906	4
GAGTACT	24165	0.0	18.358082	12-13
AGAGTAC	40435	0.0	17.954973	10-11
GTGGTAT	6810	0.0	17.850662	1
CAGAGTA	43535	0.0	17.823078	10-11
GTACTTT	27525	0.0	16.026318	14-15
TGGTATC	6735	0.0	15.816523	2
>>END_MODULE