FastQCFastQC Report
Fri 27 May 2016
SRR2138808_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138808_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10249139
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107700.044.7754671
GTATCAA189850.035.3352621
TCAACGC219850.029.6721044
ATCAACG220700.029.5010173
CAACGCA227150.028.6629055
AACGCAG235450.027.866716
TATCAAC238500.027.5638522
ACGCAGA271700.024.086687
CGCAGAG275200.023.7650838
GTACATG259150.020.6343671
TACATGG256250.020.326932
ACATGGG256800.019.6778763
GCAGAGT334750.019.4747719
GAGTACT191300.018.22486712-13
AGAGTAC294100.017.71042310-11
CAGAGTA322600.017.28512410-11
CATGGGG184000.017.053214
GTGGTAT56850.016.7729451
TATACCG6800.016.0607455
GTACTTT222850.015.76754614-15
Produced by FastQC (version 0.11.5)