##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138808_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10249139
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45361722579818	34.0	31.0	34.0	31.0	34.0
2	32.63901104278125	34.0	31.0	34.0	31.0	34.0
3	32.71805036501115	34.0	31.0	34.0	31.0	34.0
4	36.18026382508814	37.0	35.0	37.0	35.0	37.0
5	36.00178932103467	37.0	35.0	37.0	35.0	37.0
6	36.06847941080709	37.0	35.0	37.0	35.0	37.0
7	36.06735092577045	37.0	35.0	37.0	35.0	37.0
8	36.052257365228435	37.0	35.0	37.0	35.0	37.0
9	37.911800688818836	39.0	38.0	39.0	35.0	39.0
10-11	37.78662266166943	39.0	37.5	39.0	35.0	39.0
12-13	37.79140286808482	39.0	38.0	39.0	35.0	39.0
14-15	39.116443683708454	40.0	38.5	41.0	36.0	41.0
16-17	39.09039120261711	40.0	38.0	41.0	36.0	41.0
18-19	39.16067330143537	40.0	38.5	41.0	36.0	41.0
20-21	39.17809539790959	40.0	39.0	41.0	36.0	41.0
22-23	39.14165482637607	40.0	39.0	41.0	36.0	41.0
24-25	39.11192157502643	40.0	39.0	41.0	36.0	41.0
26-27	39.02144963850428	40.0	39.0	41.0	36.0	41.0
28-29	38.95879011088652	40.0	38.5	41.0	35.0	41.0
30-31	38.82819181855683	40.0	38.0	41.0	35.0	41.0
32-33	38.69975434764871	40.0	38.0	41.0	35.0	41.0
34-35	38.662051991688365	40.0	38.0	41.0	35.0	41.0
36-37	38.6156268765079	40.0	38.0	41.0	35.0	41.0
38-39	38.54703736753405	40.0	38.0	41.0	35.0	41.0
40-41	38.464584825179806	40.0	38.0	41.0	34.0	41.0
42-43	38.48527880067266	40.0	38.0	41.0	34.0	41.0
44-45	38.4302504883274	40.0	38.0	41.0	34.0	41.0
46-47	38.38293518155271	40.0	38.0	41.0	34.0	41.0
48-49	38.33983743252129	40.0	38.0	41.0	34.0	41.0
50-51	38.283538729892506	40.0	38.0	41.0	34.0	41.0
52-53	38.03366691664239	40.0	37.0	41.0	33.0	41.0
54-55	37.88982660634577	40.0	37.0	41.0	33.0	41.0
56-57	37.796998312468645	39.5	37.0	41.0	33.0	41.0
58-59	37.66795510374476	39.0	36.0	41.0	33.0	41.0
60-61	37.484834094449226	39.0	36.0	41.0	33.0	41.0
62-63	37.234452429273155	39.0	35.5	40.0	32.0	41.0
64-65	36.9970477817885	38.5	35.0	40.0	32.0	41.0
66-67	36.73342920994606	38.0	35.0	40.0	31.5	41.0
68-69	36.49314236292402	37.5	35.0	40.0	31.0	41.0
70-71	36.18575034971138	37.0	35.0	39.5	31.0	41.0
72-73	35.894037123790724	36.5	34.5	39.0	31.0	41.0
74-75	35.44477909626667	36.0	34.0	39.0	30.5	40.0
76-77	33.50907788572216	34.5	32.0	36.5	27.5	39.0
78-79	34.70542669721026	35.0	34.0	37.0	30.0	39.0
80-81	34.67868882132129	35.0	34.0	37.0	31.0	39.0
82-83	34.405165044321066	35.0	34.0	36.5	30.0	39.0
84-85	34.12096034614429	35.0	34.0	36.0	30.0	37.0
86-87	33.88674995439143	35.0	34.0	36.0	30.0	37.0
88-89	33.747173108989266	35.0	34.0	35.0	30.0	36.5
90-91	33.58066399393471	35.0	33.5	35.0	30.0	36.0
92-93	33.54752072186079	35.0	34.0	35.0	30.0	36.0
94-95	33.418964029598406	35.0	33.0	35.0	30.0	36.0
96-97	33.43822894436737	35.0	33.0	35.0	30.0	35.5
98-99	33.536856036418	35.0	33.0	35.0	30.0	35.0
100-101	32.311302668369876	34.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	1.0
19	2.0
20	32.0
21	161.0
22	767.0
23	2630.0
24	6990.0
25	15406.0
26	28943.0
27	47837.0
28	71504.0
29	99531.0
30	133170.0
31	175102.0
32	232061.0
33	318770.0
34	469373.0
35	736396.0
36	1327071.0
37	2466468.0
38	2898706.0
39	1198310.0
40	19907.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.554460482432766	20.39738366289926	14.094941447758828	23.953214406909147
2	14.517102217167649	21.31172125496462	41.8361966634475	22.334979864420234
3	18.10882220042906	26.31562873729752	30.555690800646275	25.019858261627142
4	12.42230655487143	18.0669024923664	39.383496786789465	30.1272941659727
5	13.005990374967267	38.039876250694206	35.98699516094474	12.967138213393788
6	29.654820288540567	38.47804723460489	18.936557916912868	12.930574559941677
7	26.09243566703506	32.70560580747319	23.77674846638337	17.42521005910838
8	23.85501845569662	37.46920594988515	21.515826841649822	17.159948752768404
9	25.344304531336732	16.927197494345624	21.51036296805029	36.21813500626735
10-11	23.859030876547862	27.008900650962058	29.42478674791968	19.707281724570397
12-13	24.661198805761494	24.511628721573622	29.49334823163707	21.333824241027806
14-15	21.813285230088283	25.937890340446636	26.960412782979443	25.288411646485642
16-17	21.4887113616689	29.061064436429128	27.632860634790312	21.817363567111656
18-19	21.542678928575317	28.350865782524064	29.145849673121305	20.960605615779315
20-21	22.26690578269193	27.626799468428683	28.983545487010264	21.122749261869124
22-23	21.97343475914922	27.49337664448302	28.86319307382	21.669995522547755
24-25	21.765225365969634	27.581104670299517	28.97550266886179	21.678167294869056
26-27	21.75124183918386	27.773547423175454	28.803922844515277	21.67128789312541
28-29	21.702166312244024	27.872592447171733	28.807135237082253	21.618106003501982
30-31	21.97097648181229	27.692110124997022	28.74206301448496	21.594850378705726
32-33	21.756180194730128	27.843528022435265	28.705788861794414	21.694502921040193
34-35	21.833889975143258	27.861642257187707	28.71231572341638	21.592152044252654
36-37	21.859018588217914	27.804414047824437	28.693041485696664	21.643525878260984
38-39	21.698112596838186	27.9688823102482	28.739096172783103	21.593908920130517
40-41	21.960323590928382	27.812821742079656	28.571916025486566	21.654938641505396
42-43	21.82439527992706	27.900218563493322	28.454729642458133	21.82065651412148
44-45	21.91413981684425	27.96858374643896	28.321107695123963	21.79616874159283
46-47	21.934032665338517	27.922461193445454	28.118709746443482	22.02479639477255
48-49	21.88678114758552	28.037504782854615	28.12648375489174	21.94923031466812
50-51	21.9081995342825	28.139828505783292	27.994347148719363	21.957624811214846
52-53	22.080467002229696	28.02188479523094	27.836112371001132	22.061535831538233
54-55	22.039378868344244	27.916407646986002	27.905827560159473	22.138385924510278
56-57	21.979845206430113	28.01322906450267	27.93516953555638	22.071756193510836
58-59	21.98637778780322	28.027842372264512	27.929205075523512	22.056574764408758
60-61	22.06230804732259	27.96257769218291	27.92524769930743	22.049866561187066
62-63	22.001694035264478	28.007712300780852	27.900294403778105	22.09029926017657
64-65	21.967789294166014	27.96366947521295	27.897732935657466	22.170808294963578
66-67	22.050967810011674	28.060266084982228	27.71738655390989	22.17137955109621
68-69	21.97811147892432	28.06887738409664	27.778227364362362	22.174783772616678
70-71	22.007106695449803	27.886676689589578	27.81244675712261	22.293769857838004
72-73	22.035482086382256	27.859247139137842	27.77526026368587	22.330010510794036
74-75	21.984874883232557	27.917346892744447	27.740709271489177	22.357068952533815
76-77	22.036900037023347	27.89070528475998	27.735476399298527	22.336918278918148
78-79	22.066946854514025	27.858182976287125	27.65989147169802	22.41497869750083
80-81	22.088596764026263	27.887990411340297	27.60946699879539	22.41394582583805
82-83	22.137267461377665	27.81780724510895	27.69957682174805	22.345348471765337
84-85	22.213953589258335	27.78769552986644	27.67672927046096	22.321621610414265
86-87	22.07808265564045	27.83022431940882	27.748022797446154	22.34367022750457
88-89	22.098071725882416	27.802319917109486	27.731085882499762	22.368522474508335
90-91	22.169039928466425	27.712858735096923	27.803040975490756	22.3150603609459
92-93	22.1086126873846	27.6389415540924	27.880835483043143	22.37161027547986
94-95	22.029393733671284	27.685290814218945	27.931779897560638	22.353535554549136
96-97	22.030598739376885	27.71390784095597	27.955784742144346	22.299708677522805
98-99	22.270637441281416	28.285042304948284	28.015786007338583	21.42853424643172
100-101	26.38961387749621	35.282445561569745	28.67783506259187	9.65010549834217
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1906.0
1	1704.5
2	2394.0
3	3555.0
4	4794.5
5	7598.5
6	10137.0
7	12502.5
8	14388.0
9	14357.0
10	12763.5
11	11138.0
12	11954.0
13	15601.5
14	23918.5
15	34983.5
16	43467.5
17	47900.5
18	49049.0
19	47791.5
20	45689.0
21	45028.5
22	47396.5
23	54039.5
24	66055.0
25	84163.5
26	106662.5
27	129976.0
28	153285.0
29	177143.0
30	198437.0
31	218288.0
32	239704.0
33	260305.0
34	280677.5
35	300676.0
36	322952.0
37	343151.0
38	358340.0
39	370947.5
40	384555.5
41	399696.5
42	410058.5
43	418794.5
44	426233.0
45	432478.5
46	436204.5
47	436411.0
48	433831.5
49	426682.0
50	416541.5
51	403379.0
52	383957.5
53	361575.0
54	339670.5
55	314775.5
56	287741.5
57	259505.5
58	229967.0
59	199684.5
60	168002.0
61	136403.0
62	107682.5
63	82747.0
64	61700.5
65	45863.0
66	34276.5
67	25740.0
68	19472.0
69	14054.5
70	9902.0
71	7165.5
72	5029.0
73	3394.0
74	2290.0
75	1583.0
76	1074.5
77	724.5
78	507.0
79	371.0
80	275.0
81	189.0
82	133.5
83	94.5
84	69.0
85	49.5
86	33.5
87	24.5
88	19.0
89	15.0
90	10.5
91	9.5
92	8.5
93	4.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.756917142015539E-6
2	0.0011415593056158181
3	0.002497770788355978
4	4.293043542486837E-4
5	3.024644314024817E-4
6	4.195474371066682E-4
7	0.0
8	0.0
9	0.0
10-11	9.756917142015539E-6
12-13	9.512994213465151E-4
14-15	7.122549513671343E-4
16-17	6.3419961423101E-5
18-19	4.8784585710077695E-6
20-21	9.757181798055414E-6
22-23	9.757985850237459E-6
24-25	9.759872489217903E-6
26-27	4.881488920509005E-5
28-29	2.1487513142178134E-4
30-31	2.9341671193026972E-5
32-33	0.001171942729953797
34-35	0.0017221009316467635
36-37	0.0013795377955035475
38-39	6.423677695905489E-4
40-41	2.50895408077116E-5
42-43	4.5532986371977176E-5
44-45	5.873747225484422E-4
46-47	5.1634841704098485E-6
48-49	0.0
50-51	0.0
52-53	9.207372433584642E-4
54-55	0.003415586491913948
56-57	0.006296213925500659
58-59	0.004518914450486035
60-61	2.047749307667028E-4
62-63	3.355410067460519E-5
64-65	8.475126436173738E-5
66-67	1.4282342020729735E-4
68-69	0.0
70-71	1.3456972879694488E-4
72-73	1.184247962575396E-5
74-75	0.0
76-77	0.0
78-79	1.2272790756526733E-5
80-81	0.0
82-83	0.0
84-85	6.379570675308178E-6
86-87	2.5853593555784978E-5
88-89	3.473859697361276E-4
90-91	1.1978146271399375E-4
92-93	6.628669509689491E-4
94-95	3.9249699980802076E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	163.0
20-21	667.0
22-23	1396.0
24-25	3168.0
26-27	3996.0
28-29	9127.0
30-31	26403.0
32-33	32570.0
34-35	43886.0
36-37	66624.0
38-39	77423.0
40-41	79736.0
42-43	89361.0
44-45	104653.0
46-47	105128.0
48-49	98604.0
50-51	91043.0
52-53	82860.0
54-55	89537.0
56-57	91409.0
58-59	92885.0
60-61	95537.0
62-63	90226.0
64-65	95617.0
66-67	101472.0
68-69	103968.0
70-71	102987.0
72-73	98052.0
74-75	99257.0
76-77	97444.0
78-79	103842.0
80-81	105922.0
82-83	101502.0
84-85	100932.0
86-87	105711.0
88-89	112904.0
90-91	120779.0
92-93	125366.0
94-95	145817.0
96-97	206953.0
98-99	852822.0
100-101	6091390.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.42506484252894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.39598861007427	78.12479177130177
2	6.8015166300669865	11.756430315621564
3	1.6158031290919834	4.189376706136075
4	0.563629594200288	1.9484689690371266
5	0.2697003091336042	1.1654433352460924
6	0.12047391525357598	0.6247179562574185
7	0.07720173358340135	0.4670515381630774
8	0.03985899098215188	0.2755852704152202
9	0.028633485002900444	0.22271857182389257
>10	0.08588195163577976	1.1002015546236965
>50	0.0010491183013562795	0.062043434575419505
>100	2.1133755996312716E-4	0.03019340097939745
>500	5.1195113722767674E-5	0.03297717581923746
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.756917142015539E-6	0.0
2	0.0	1.9513834284031078E-5	0.0	9.756917142015539E-6	0.0
3	0.0	1.9513834284031078E-5	0.0	9.756917142015539E-6	0.0
4	0.0	2.9270751426046616E-5	0.0	9.756917142015539E-6	0.0
5	0.0	2.9270751426046616E-5	0.0	9.756917142015539E-6	0.0
6	9.756917142015539E-6	2.9270751426046616E-5	0.0	9.756917142015539E-6	0.0
7	9.756917142015539E-6	2.9270751426046616E-5	0.0	1.9513834284031078E-5	0.0
8	9.756917142015539E-6	3.9027668568062156E-5	0.0	1.9513834284031078E-5	0.0
9	9.756917142015539E-6	9.756917142015539E-5	0.0	2.9270751426046616E-5	9.756917142015539E-6
10-11	9.756917142015539E-6	9.756917142015539E-5	0.0	5.366304428108546E-5	9.756917142015539E-6
12-13	9.756917142015539E-6	9.756917142015539E-5	0.0	7.805533713612431E-5	9.756917142015539E-6
14-15	9.756917142015539E-6	1.5611067427224863E-4	0.0	8.293379570713209E-5	3.9027668568062156E-5
16-17	1.9513834284031078E-5	1.5611067427224863E-4	0.0	1.3171838141720976E-4	3.9027668568062156E-5
18-19	1.9513834284031078E-5	1.6098913284325641E-4	0.0	1.90259884269303E-4	3.9027668568062156E-5
20-21	1.9513834284031078E-5	1.6586759141426418E-4	0.0	3.366136413995361E-4	3.9027668568062156E-5
22-23	1.9513834284031078E-5	2.097737185533341E-4	0.0	7.464041613641887E-4	4.3906127139069925E-5
24-25	1.9513834284031078E-5	2.53679845692404E-4	0.0	0.0018391788812699291	4.8784585710077694E-5
26-27	1.9513834284031078E-5	2.731936799764351E-4	0.0	0.004668684852454435	4.8784585710077694E-5
28-29	1.9513834284031078E-5	2.731936799764351E-4	0.0	0.030680625953067864	4.8784585710077694E-5
30-31	1.9513834284031078E-5	3.219782656865128E-4	0.0	0.04422810540475644	4.8784585710077694E-5
32-33	2.4392292855038847E-5	3.219782656865128E-4	0.0	0.04990175272283848	5.854150285209323E-5
34-35	2.9270751426046616E-5	4.049120613936449E-4	0.0	0.05671695934653633	7.317687856511654E-5
36-37	2.9270751426046616E-5	4.7808893995876143E-4	0.0	0.06683000396423544	7.805533713612431E-5
38-39	2.9270751426046616E-5	4.976027742427925E-4	0.0	0.08642677204397364	7.805533713612431E-5
40-41	3.414920999705439E-5	5.659011942369013E-4	0.0	0.12761071930042123	8.781225427813985E-5
42-43	3.9027668568062156E-5	5.951719456629479E-4	0.0	0.1445438490003892	8.781225427813985E-5
44-45	3.9027668568062156E-5	6.634703656570567E-4	0.0	0.16684328312846572	8.781225427813985E-5
46-47	3.9027668568062156E-5	7.024980342251188E-4	0.0	0.192123455443428	9.269071284914761E-5
48-49	3.9027668568062156E-5	7.024980342251188E-4	0.0	0.2150424538100225	9.756917142015539E-5
50-51	3.9027668568062156E-5	7.756749127902354E-4	0.0	0.23757605394950737	9.756917142015539E-5
52-53	3.9027668568062156E-5	7.903102885032586E-4	0.0	0.2678761601340366	1.0732608856217092E-4
54-55	3.9027668568062156E-5	7.903102885032586E-4	0.0	0.32873980926592955	1.1708300570418646E-4
56-57	3.9027668568062156E-5	7.903102885032586E-4	0.0	0.3933208438289304	1.1708300570418646E-4
58-59	3.9027668568062156E-5	8.000672056452743E-4	0.0	0.458999531570408	1.1708300570418646E-4
60-61	3.9027668568062156E-5	8.293379570713208E-4	0.0	0.5022421883438208	1.1708300570418646E-4
62-63	3.9027668568062156E-5	8.537302499263596E-4	0.0	0.5220633655178255	1.1708300570418646E-4
64-65	3.9027668568062156E-5	9.366640456334917E-4	0.0	0.5400404853519891	1.2196146427519424E-4
66-67	4.8784585710077694E-5	9.415425042044995E-4	0.0	0.5478655328998856	1.26839922846202E-4
68-69	4.8784585710077694E-5	9.708132556305461E-4	0.0	0.5554417790606606	1.26839922846202E-4
70-71	5.854150285209324E-5	9.805701727725617E-4	0.0	0.5624423671100567	1.26839922846202E-4
72-73	8.781225427813985E-5	9.854486313435695E-4	0.0	0.5678233069138783	1.26839922846202E-4
74-75	8.781225427813985E-5	0.0010000840070565928	0.0	0.5722285550034983	1.26839922846202E-4
76-77	8.781225427813985E-5	0.0010147193827696162	0.0	0.5760093603960293	1.26839922846202E-4
78-79	8.781225427813985E-5	0.0010244762999116316	0.0	0.5793511045171696	1.26839922846202E-4
80-81	8.781225427813985E-5	0.001034233217053647	0.0	0.5809561173870312	1.26839922846202E-4
82-83	8.781225427813985E-5	0.0010391116756246549	0.0	0.5815268970398391	1.26839922846202E-4
84-85	8.781225427813985E-5	0.0010439901341956627	0.0	0.5820976766926471	1.26839922846202E-4
86-87	8.781225427813985E-5	0.0010537470513376783	0.0	0.5823806272897655	1.26839922846202E-4
88-89	8.781225427813985E-5	0.001122045471331787	0.0	0.5824879533783277	1.26839922846202E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10770	0.0	44.775467	1
GTATCAA	18985	0.0	35.335262	1
TCAACGC	21985	0.0	29.672104	4
ATCAACG	22070	0.0	29.501017	3
CAACGCA	22715	0.0	28.662905	5
AACGCAG	23545	0.0	27.86671	6
TATCAAC	23850	0.0	27.563852	2
ACGCAGA	27170	0.0	24.08668	7
CGCAGAG	27520	0.0	23.765083	8
GTACATG	25915	0.0	20.634367	1
TACATGG	25625	0.0	20.32693	2
ACATGGG	25680	0.0	19.677876	3
GCAGAGT	33475	0.0	19.474771	9
GAGTACT	19130	0.0	18.224867	12-13
AGAGTAC	29410	0.0	17.710423	10-11
CAGAGTA	32260	0.0	17.285124	10-11
CATGGGG	18400	0.0	17.05321	4
GTGGTAT	5685	0.0	16.772945	1
TATACCG	680	0.0	16.060745	5
GTACTTT	22285	0.0	15.767546	14-15
>>END_MODULE