FastQCFastQC Report
Fri 27 May 2016
SRR2138807_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138807_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12378094
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA137600.044.9474641
GTATCAA245300.035.3558961
TCAACGC291000.029.145794
ATCAACG293000.028.9193123
CAACGCA297300.028.5698535
AACGCAG307850.027.6856566
TATCAAC313950.027.386642
ACGCAGA357650.023.807617
CGCAGAG361050.023.5604068
GTACATG331500.021.0501231
TACATGG328100.020.6479362
ACATGGG331350.019.918763
GCAGAGT442250.019.2725169
CATGGGG230750.018.1619554
GAGTACT255050.017.84079412-13
AGAGTAC387650.017.78145410-11
CAGAGTA425400.017.11158810-11
GTGGTAT75550.017.0983731
TGGTATC71850.015.839912
AGTACTT284200.015.616281512-13
Produced by FastQC (version 0.11.5)