##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138807_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12378094
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45429837582426	34.0	31.0	34.0	31.0	34.0
2	32.64655721632103	34.0	31.0	34.0	31.0	34.0
3	32.72825517401952	34.0	31.0	34.0	31.0	34.0
4	36.189523443593174	37.0	37.0	37.0	35.0	37.0
5	36.00518803621947	37.0	35.0	37.0	35.0	37.0
6	36.07382348203205	37.0	35.0	37.0	35.0	37.0
7	36.075049680508165	37.0	35.0	37.0	35.0	37.0
8	36.063701729846294	37.0	35.0	37.0	35.0	37.0
9	37.92953163871594	39.0	38.0	39.0	35.0	39.0
10-11	37.80782065477932	39.0	38.0	39.0	35.0	39.0
12-13	37.81469744049447	39.0	38.0	39.0	35.0	39.0
14-15	39.136666800235965	40.0	39.0	41.0	36.0	41.0
16-17	39.11267380906948	40.0	38.5	41.0	36.0	41.0
18-19	39.18705791861009	40.0	39.0	41.0	36.0	41.0
20-21	39.21006054165208	40.0	39.0	41.0	36.0	41.0
22-23	39.17081637049053	40.0	39.0	41.0	36.0	41.0
24-25	39.140910782734395	40.0	39.0	41.0	36.0	41.0
26-27	39.05264448269752	40.0	39.0	41.0	36.0	41.0
28-29	38.99049470209323	40.0	39.0	41.0	35.5	41.0
30-31	38.85731126842368	40.0	38.0	41.0	35.0	41.0
32-33	38.72202012530886	40.0	38.0	41.0	35.0	41.0
34-35	38.68648652380447	40.0	38.0	41.0	35.0	41.0
36-37	38.640573980455834	40.0	38.0	41.0	35.0	41.0
38-39	38.576918680975	40.0	38.0	41.0	35.0	41.0
40-41	38.49419197169451	40.0	38.0	41.0	34.0	41.0
42-43	38.52142114545447	40.0	38.0	41.0	34.0	41.0
44-45	38.466864467442264	40.0	38.0	41.0	34.0	41.0
46-47	38.42256884596269	40.0	38.0	41.0	34.0	41.0
48-49	38.38554446871332	40.0	38.0	41.0	34.0	41.0
50-51	38.33322619923993	40.0	38.0	41.0	34.0	41.0
52-53	38.091717716322634	40.0	37.0	41.0	33.5	41.0
54-55	37.95095564540927	40.0	37.0	41.0	33.0	41.0
56-57	37.866741473509904	40.0	37.0	41.0	33.0	41.0
58-59	37.741393906828606	39.0	36.5	41.0	33.0	41.0
60-61	37.56526285478063	39.0	36.0	41.0	33.0	41.0
62-63	37.3244584447459	39.0	35.5	40.5	32.5	41.0
64-65	37.091776075805704	39.0	35.0	40.0	32.0	41.0
66-67	36.836204120982146	38.0	35.0	40.0	32.0	41.0
68-69	36.60113802185599	37.5	35.0	40.0	31.5	41.0
70-71	36.297825452942185	37.0	35.0	39.5	31.0	41.0
72-73	36.01254154266646	36.5	35.0	39.0	31.0	41.0
74-75	35.56263185017445	36.0	34.0	39.0	31.0	40.0
76-77	33.621693974220605	34.5	32.0	36.5	28.0	39.0
78-79	34.81494283158784	35.0	34.0	37.0	30.5	39.0
80-81	34.78405374336185	35.0	34.0	37.0	31.0	39.0
82-83	34.50973997965995	35.0	34.0	36.5	31.0	39.0
84-85	34.22368792032442	35.0	34.0	36.0	30.0	37.5
86-87	33.98166716946936	35.0	34.0	36.0	30.0	37.0
88-89	33.83832233244392	35.0	34.0	35.0	30.0	37.0
90-91	33.6668049594314	35.0	34.0	35.0	30.0	36.0
92-93	33.62300449542589	35.0	34.0	35.0	30.0	36.0
94-95	33.48583577035712	35.0	33.5	35.0	30.0	36.0
96-97	33.50144903530433	35.0	33.5	35.0	30.0	36.0
98-99	33.59177544791997	35.0	34.0	35.0	30.5	35.0
100-101	32.367773111679085	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	2.0
19	14.0
20	48.0
21	192.0
22	866.0
23	2976.0
24	7891.0
25	17297.0
26	32275.0
27	53166.0
28	80808.0
29	113335.0
30	153526.0
31	203308.0
32	270665.0
33	374553.0
34	551568.0
35	865919.0
36	1561917.0
37	2933170.0
38	3565069.0
39	1563079.0
40	26448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.38549925376233	20.660159795199487	14.15957093232609	23.79477001871209
2	14.37325915644226	21.541093259246928	41.99305072338546	22.092596860925358
3	17.884655810569335	26.728266705747366	30.737999933106074	24.64907755057722
4	12.26687023162246	18.326121497029696	39.648107198955955	29.758901072391886
5	12.849017766032622	38.159876277855844	36.10011068779039	12.890995268321149
6	29.169632269551045	38.75722105788367	19.200666277051752	12.872480395513536
7	25.73763779787098	32.93340638712228	24.098241619428645	17.23071419557809
8	23.66596989811194	37.55600821903599	21.74496331987784	17.033058562974233
9	25.306755628128208	16.905340999995637	21.811443668144708	35.97645970373144
10-11	23.6763349833989	27.15152672131913	29.57005335393317	19.602084941348803
12-13	24.537237194817095	24.74142259150564	29.631688694584852	21.089651519092413
14-15	21.71362710381186	26.116625550734817	27.194064666948027	24.975682678505297
16-17	21.274919996721625	29.180952160901448	27.892909780522217	21.651218061854713
18-19	21.29039818246654	28.475054398520484	29.486062232198268	20.748485186814708
20-21	22.092603051953137	27.70597443970465	29.30622159360134	20.895200914740876
22-23	21.80638872134676	27.588787115743308	29.15972045758729	21.44510370532264
24-25	21.522786028480617	27.746122322315365	29.2513675819438	21.47972406726022
26-27	21.527497858873012	27.893743026280283	29.11546677053376	21.463292344312947
28-29	21.46556529011047	28.009852409236608	29.112662255934257	21.411920044718663
30-31	21.79392211105677	27.856844457065616	28.974112689239433	21.375120742638178
32-33	21.55082980385272	28.032679292332023	28.922250642296387	21.49424026151887
34-35	21.64274468755261	28.05467790027353	28.958483685810325	21.344093726363532
36-37	21.64945417580366	27.978682684991075	28.940117660068125	21.431745479137142
38-39	21.503814753909676	28.077948116171726	28.992795327571404	21.425441802347194
40-41	21.77439775854517	27.938285915541776	28.822414114485156	21.464902211427894
42-43	21.600185848749206	28.088139080798786	28.690796332341108	21.620878738110903
44-45	21.72710095021108	28.17527334155954	28.49322006229683	21.60440564593255
46-47	21.766214255666537	28.126633277324594	28.316369069254577	21.7907833977543
48-49	21.763737835386426	28.228454130929244	28.269653875510876	21.738154158173458
50-51	21.75689464651806	28.314022674852545	28.14499894470371	21.784083733925684
52-53	21.918846117175743	28.209826930051157	28.03939450913021	21.83193244364289
54-55	21.877182197715467	28.116711926396636	28.08444894650226	21.921656929385634
56-57	21.86452815059474	28.200984634224426	28.084396516557092	21.85009069862375
58-59	21.8561269999142	28.276699107417773	28.02870942421622	21.838464468451807
60-61	21.92388964154679	28.23937169492188	28.03282980939308	21.803908854138253
62-63	21.912472956062167	28.232786369091308	28.000810056548325	21.853930618298204
64-65	21.878521337297624	28.20101679251717	28.01306267520677	21.90739919497844
66-67	21.96125851415339	28.224390726188997	27.848356394017436	21.965994365640178
68-69	21.89811206531673	28.230139278470745	27.942013172806192	21.929735483406336
70-71	21.904791764054306	28.004856281352875	28.020799869875752	22.069552084717067
72-73	22.005844300803403	28.01051472487891	27.976763260668356	22.006877713649327
74-75	21.87390094925476	28.11752505810302	27.944739804720207	22.06383418792201
76-77	21.942254152622663	28.061287985053568	27.939908676127974	22.0565491861958
78-79	21.91906738852588	28.07185846711485	27.885377243088357	22.12369690127091
80-81	21.90914185478201	28.09399439174652	27.92304121323861	22.073822540232864
82-83	21.97567293434305	28.000956368679695	27.99868440364052	22.02468629333673
84-85	22.123324504548744	27.9853640624287	27.88882141157708	22.00249002144547
86-87	21.93147993733367	27.98865585660379	27.986292814711188	22.093571391351354
88-89	21.968116652887524	27.950508233189275	27.941122129642604	22.140252984280597
90-91	22.011688847783716	27.943198074751287	28.001851323017814	22.043261754447187
92-93	22.04908924668497	27.863111920145865	28.00569620852763	22.082102624641532
94-95	21.93086033178604	27.88726452885125	28.064631242221644	22.11724389714107
96-97	21.94297042158382	27.895347226635316	28.08857559638011	22.073106755400755
98-99	22.201060428549173	28.450669110328946	28.10562035646147	21.242650104660406
100-101	26.155580745866462	35.42617264297112	28.846905637788815	9.571340973373607
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2490.0
1	2255.0
2	3362.5
3	4953.0
4	6683.5
5	10699.0
6	14255.5
7	17481.0
8	20037.5
9	19851.0
10	17470.5
11	15296.0
12	16600.0
13	21807.0
14	33010.0
15	47506.5
16	58715.5
17	64724.5
18	65979.5
19	63966.5
20	61294.5
21	60327.0
22	63391.0
23	72779.5
24	88772.5
25	110895.5
26	138671.5
27	168423.5
28	198497.5
29	229475.5
30	256744.5
31	281449.0
32	307390.0
33	333519.5
34	358718.0
35	382469.0
36	408249.5
37	431375.0
38	449243.0
39	464111.5
40	478999.0
41	492337.5
42	500532.5
43	509068.0
44	517233.5
45	524495.5
46	527796.0
47	526790.0
48	523463.0
49	513329.5
50	500916.0
51	485328.0
52	460753.0
53	432296.5
54	403347.0
55	371271.0
56	337389.5
57	302550.0
58	266392.0
59	230348.0
60	193098.5
61	156493.0
62	123886.5
63	95960.5
64	72101.0
65	53725.0
66	40152.5
67	30504.0
68	23450.5
69	16933.0
70	11902.0
71	8546.5
72	5987.0
73	3991.5
74	2689.5
75	1846.5
76	1283.0
77	876.0
78	594.5
79	427.5
80	307.0
81	212.5
82	157.5
83	113.0
84	77.5
85	55.0
86	39.5
87	28.0
88	20.0
89	14.0
90	8.5
91	7.0
92	6.0
93	3.5
94	1.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	9.290606453626867E-4
3	0.0023266910075169893
4	5.089636578943414E-4
5	2.8275758771907855E-4
6	4.8472729323270614E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0010300454981195003
14-15	6.947757869668787E-4
16-17	6.059091165408827E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.254792562819327E-5
28-29	2.3461144118289146E-4
30-31	4.051594790767339E-5
32-33	0.001133809931760491
34-35	0.0021742886975659875
36-37	0.0014683503641467887
38-39	8.557821458129578E-4
40-41	3.75388652384769E-5
42-43	5.051150262670337E-5
44-45	7.743116082626536E-4
46-47	2.153298579955572E-5
48-49	0.0
50-51	0.0
52-53	0.0011940017628455878
54-55	0.0041154431328157964
56-57	0.007213651179069919
58-59	0.005216003842517601
60-61	3.263630880736484E-4
62-63	2.358105371749888E-5
64-65	1.3833113879726706E-4
66-67	2.558608425980303E-4
68-69	0.0
70-71	1.337298274127424E-4
72-73	1.504969937473014E-5
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	1.1016511106185508E-5
88-89	3.242359979662577E-4
90-91	1.7038776506798643E-4
92-93	6.06541322880489E-4
94-95	3.9408134521255273E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	232.0
20-21	843.0
22-23	1884.0
24-25	4137.0
26-27	7139.0
28-29	14765.0
30-31	35560.0
32-33	43525.0
34-35	58974.0
36-37	89689.0
38-39	107580.0
40-41	106995.0
42-43	120350.0
44-45	140526.0
46-47	140152.0
48-49	131906.0
50-51	122227.0
52-53	116496.0
54-55	124123.0
56-57	127773.0
58-59	127499.0
60-61	124077.0
62-63	119582.0
64-65	122664.0
66-67	130119.0
68-69	131574.0
70-71	129852.0
72-73	123336.0
74-75	125366.0
76-77	123428.0
78-79	130536.0
80-81	132098.0
82-83	126921.0
84-85	126482.0
86-87	131509.0
88-89	138505.0
90-91	147717.0
92-93	149255.0
94-95	171229.0
96-97	243179.0
98-99	983687.0
100-101	7144603.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.60085505961653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.64848602789014	75.84338572759592
2	7.04552391631978	11.921146953272633
3	1.788334064956882	4.53883773082776
4	0.6857740382175179	2.320682800435527
5	0.3393935839684398	1.4356493702738884
6	0.16706585954716743	0.84803487413761
7	0.10204457416398224	0.6043140759927146
8	0.058947772298196144	0.39896255522295765
9	0.03883668121098831	0.2957054794314619
>10	0.12298738650468374	1.566534496425303
>50	0.002069205404190572	0.11583552191230825
>100	4.7382841718385254E-4	0.05914887449130001
>500	3.15330035920363E-5	0.019993949968652662
>1k	3.1528097250091335E-5	0.031767590011993946
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6157576441090202E-5
2	0.0	8.078788220545101E-6	0.0	0.0	1.6157576441090202E-5
3	0.0	8.078788220545101E-6	0.0	0.0	1.6157576441090202E-5
4	0.0	9.694545864654122E-5	0.0	8.078788220545101E-6	1.6157576441090202E-5
5	0.0	9.694545864654122E-5	0.0	8.078788220545101E-6	1.6157576441090202E-5
6	2.4236364661635306E-5	9.694545864654122E-5	0.0	8.078788220545101E-6	5.6551517543815713E-5
7	2.4236364661635306E-5	9.694545864654122E-5	0.0	2.4236364661635306E-5	5.6551517543815713E-5
8	2.4236364661635306E-5	9.694545864654122E-5	0.0	2.4236364661635306E-5	7.270909398490592E-5
9	2.4236364661635306E-5	1.9389091729308245E-4	0.0	4.039394110272551E-5	9.694545864654122E-5
10-11	2.4236364661635306E-5	1.9389091729308245E-4	0.0	4.847272932327061E-5	9.694545864654122E-5
12-13	2.4236364661635306E-5	1.9389091729308245E-4	0.0	4.847272932327061E-5	1.0502424686708632E-4
14-15	2.4236364661635306E-5	2.6660001127798834E-4	0.0	7.270909398490592E-5	1.3733939974926672E-4
16-17	6.059091165408826E-5	2.6660001127798834E-4	0.0	8.078788220545102E-5	1.7773334085199223E-4
18-19	6.463030576436081E-5	2.8275758771907855E-4	0.0	8.482727631572358E-5	2.1812728195471775E-4
20-21	6.463030576436081E-5	3.1103334649098646E-4	0.0	9.290606453626867E-5	2.221666760649903E-4
22-23	6.463030576436081E-5	3.59506075814257E-4	0.0	9.694545864654122E-5	2.4236364661635304E-4
24-25	6.463030576436081E-5	3.918212286964374E-4	0.0	1.4137879385953928E-4	2.7063940538826086E-4
26-27	6.463030576436081E-5	3.9586062280671E-4	0.0	1.898515231828099E-4	2.8275758771907855E-4
28-29	6.463030576436081E-5	3.9586062280671E-4	0.0	6.947757869668787E-4	2.9487577004989624E-4
30-31	6.463030576436081E-5	4.1201819924780017E-4	0.0	0.0019106334141589165	3.029545582704413E-4
32-33	6.866969987463336E-5	4.1201819924780017E-4	0.0	0.004390821397866263	3.756636522553472E-4
34-35	8.078788220545102E-5	4.887666873429786E-4	0.0	0.00736381546302686	4.0797880513752764E-4
36-37	8.886667042599612E-5	5.695545695484297E-4	0.0	0.01213030051314847	4.1201819924780017E-4
38-39	8.886667042599612E-5	5.816727518792473E-4	0.0	0.018771064430436545	4.322151697991629E-4
40-41	8.886667042599612E-5	6.139879047614278E-4	0.0	0.026009658676044954	4.483727462402531E-4
42-43	1.1310303508763143E-4	6.38224269423063E-4	0.0	0.03246057107015022	4.604909285710708E-4
44-45	1.2118182330817652E-4	7.392091221798768E-4	0.0	0.03963453500999427	4.7260911090188845E-4
46-47	1.2926061152872162E-4	8.240363984956003E-4	0.0	0.047470959583923014	4.887666873429786E-4
48-49	1.2926061152872162E-4	8.240363984956003E-4	0.0	0.054794381105847154	5.089636578943414E-4
50-51	1.6561515852117457E-4	9.209818571421415E-4	0.0	0.06187947837526521	5.089636578943414E-4
52-53	1.7773334085199223E-4	9.290606453626867E-4	0.0	0.06886763018603673	5.533969931073395E-4
54-55	1.7773334085199223E-4	9.290606453626867E-4	0.0	0.07604159412588077	6.0186972243061E-4
56-57	1.7773334085199223E-4	9.290606453626867E-4	0.0	0.08342964595356928	6.139879047614278E-4
58-59	1.7773334085199223E-4	9.371394335832318E-4	0.0	0.09059957049930303	6.220666929819728E-4
60-61	2.0196970551362753E-4	9.654151923551397E-4	0.0	0.09764831322172865	6.341848753127904E-4
62-63	2.1812728195471775E-4	9.89651557016775E-4	0.0	0.10434966805067081	6.624606340846983E-4
64-65	2.1812728195471775E-4	0.001114872774435224	0.0	0.11076422589778362	6.665000281949709E-4
66-67	2.1812728195471775E-4	0.0011189121685454967	0.0	0.1171101140450218	6.866969987463337E-4
68-69	2.1812728195471775E-4	0.0011673848978687672	0.0	0.12394476887960296	7.028545751874239E-4
70-71	2.221666760649903E-4	0.00118758186842013	0.0	0.13024218429751785	7.109333634079689E-4
72-73	2.3428485839580796E-4	0.00118758186842013	0.0	0.13529546632946882	7.513273045106944E-4
74-75	2.3428485839580796E-4	0.0012077788389714926	0.0	0.13940756953372627	7.513273045106944E-4
76-77	2.3428485839580796E-4	0.001223936415412583	0.0	0.14305918180941268	7.513273045106944E-4
78-79	2.3428485839580796E-4	0.0012360545977434005	0.0	0.14669059711454768	7.513273045106944E-4
80-81	2.3428485839580796E-4	0.0012360545977434005	0.0	0.14850832446417034	7.715242750620572E-4
82-83	2.3428485839580796E-4	0.0012360545977434005	0.0	0.14917886388647555	8.159576102750553E-4
84-85	2.3428485839580796E-4	0.0012522121741844908	0.0	0.14984132452056026	8.159576102750553E-4
86-87	2.3428485839580796E-4	0.001276448538846126	0.0	0.15007964877306634	8.199970043853279E-4
88-89	2.4236364661635304E-4	0.001357236421051577	0.0	0.15016043665527182	8.401939749366906E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13760	0.0	44.947464	1
GTATCAA	24530	0.0	35.355896	1
TCAACGC	29100	0.0	29.14579	4
ATCAACG	29300	0.0	28.919312	3
CAACGCA	29730	0.0	28.569853	5
AACGCAG	30785	0.0	27.685656	6
TATCAAC	31395	0.0	27.38664	2
ACGCAGA	35765	0.0	23.80761	7
CGCAGAG	36105	0.0	23.560406	8
GTACATG	33150	0.0	21.050123	1
TACATGG	32810	0.0	20.647936	2
ACATGGG	33135	0.0	19.91876	3
GCAGAGT	44225	0.0	19.272516	9
CATGGGG	23075	0.0	18.161955	4
GAGTACT	25505	0.0	17.840794	12-13
AGAGTAC	38765	0.0	17.781454	10-11
CAGAGTA	42540	0.0	17.111588	10-11
GTGGTAT	7555	0.0	17.098373	1
TGGTATC	7185	0.0	15.83991	2
AGTACTT	28420	0.0	15.6162815	12-13
>>END_MODULE