FastQCFastQC Report
Fri 27 May 2016
SRR2138806_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138806_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12066596
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133250.046.2747731
GTATCAA241700.035.3446851
TCAACGC285150.029.1268584
ATCAACG285800.029.0906523
CAACGCA290100.028.5579015
AACGCAG298750.027.8844766
TATCAAC303700.027.6092132
ACGCAGA352850.023.503177
CGCAGAG357200.023.1936848
GTACATG382850.021.0656341
TACATGG370850.020.9629862
ACATGGG374000.020.2082963
GCAGAGT434750.018.9708149
AGAGTAC380000.017.68041210-11
CATGGGG248550.017.6524264
GAGTACT226600.017.38247712-13
CAGAGTA417950.017.1536510-11
GTGGTAT72750.017.134281
TGGTATC68550.016.0626072
GTACTTT261450.015.11346614-15
Produced by FastQC (version 0.11.5)