##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138806_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12066596
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.430737881669366	33.0	31.0	34.0	31.0	34.0
2	32.62454324318142	34.0	31.0	34.0	31.0	34.0
3	32.70626861129684	34.0	31.0	34.0	31.0	34.0
4	36.17176973522607	37.0	35.0	37.0	35.0	37.0
5	35.98466941298109	37.0	35.0	37.0	35.0	37.0
6	36.05223751586612	37.0	35.0	37.0	35.0	37.0
7	36.05289113847849	37.0	35.0	37.0	35.0	37.0
8	36.03942031373222	37.0	35.0	37.0	35.0	37.0
9	37.8990387181273	39.0	38.0	39.0	35.0	39.0
10-11	37.776724645459254	39.0	37.5	39.0	35.0	39.0
12-13	37.78183138807332	39.0	38.0	39.0	35.0	39.0
14-15	39.09750985282014	40.0	38.5	41.0	36.0	41.0
16-17	39.069951790877894	40.0	38.0	41.0	36.0	41.0
18-19	39.14276536647121	40.0	38.5	41.0	36.0	41.0
20-21	39.160902064235756	40.0	39.0	41.0	36.0	41.0
22-23	39.12233472523319	40.0	39.0	41.0	36.0	41.0
24-25	39.09165452061978	40.0	39.0	41.0	36.0	41.0
26-27	39.00013812258466	40.0	39.0	41.0	35.5	41.0
28-29	38.936343294503345	40.0	38.0	41.0	35.0	41.0
30-31	38.804209335964444	40.0	38.0	41.0	35.0	41.0
32-33	38.672629967419994	40.0	38.0	41.0	35.0	41.0
34-35	38.63636910634331	40.0	38.0	41.0	35.0	41.0
36-37	38.59284584162212	40.0	38.0	41.0	35.0	41.0
38-39	38.52694650926125	40.0	38.0	41.0	34.5	41.0
40-41	38.445277795674066	40.0	38.0	41.0	34.0	41.0
42-43	38.46919412820333	40.0	38.0	41.0	34.0	41.0
44-45	38.41614296349269	40.0	38.0	41.0	34.0	41.0
46-47	38.37226599774024	40.0	38.0	41.0	34.0	41.0
48-49	38.33237831944637	40.0	38.0	41.0	34.0	41.0
50-51	38.275798167480986	40.0	38.0	41.0	34.0	41.0
52-53	38.03140125129353	40.0	37.0	41.0	33.0	41.0
54-55	37.88670870520765	40.0	37.0	41.0	33.0	41.0
56-57	37.79554577980251	39.5	37.0	41.0	33.0	41.0
58-59	37.66975463532628	39.0	36.0	41.0	33.0	41.0
60-61	37.48996526259535	39.0	36.0	41.0	33.0	41.0
62-63	37.24641722426987	39.0	35.5	40.0	32.0	41.0
64-65	37.00944307382524	38.5	35.0	40.0	32.0	41.0
66-67	36.74925021067976	38.0	35.0	40.0	32.0	41.0
68-69	36.50961688484924	37.5	35.0	40.0	31.5	41.0
70-71	36.20200033212794	37.0	35.0	39.5	31.0	41.0
72-73	35.90704951791414	36.5	34.5	39.0	31.0	41.0
74-75	35.45669932211037	36.0	34.0	39.0	31.0	40.0
76-77	33.51493633317427	34.5	32.0	36.5	28.0	39.0
78-79	34.710596181243744	35.0	34.0	37.0	30.0	39.0
80-81	34.6819272587078	35.0	34.0	37.0	31.0	39.0
82-83	34.409857516109916	35.0	34.0	36.5	30.5	38.5
84-85	34.1291287394103	35.0	34.0	36.0	30.0	37.0
86-87	33.8972444584753	35.0	34.0	36.0	30.0	37.0
88-89	33.75685881293287	35.0	34.0	35.0	30.0	36.5
90-91	33.589257110050625	35.0	34.0	35.0	30.0	36.0
92-93	33.55357433132189	35.0	34.0	35.0	30.0	36.0
94-95	33.42172215925655	35.0	33.0	35.0	30.0	36.0
96-97	33.44249155911331	35.0	33.0	35.0	30.0	35.5
98-99	33.53849753858033	35.0	33.0	35.0	30.0	35.0
100-101	32.30973903758185	34.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	8.0
20	43.0
21	224.0
22	930.0
23	2989.0
24	8100.0
25	17876.0
26	33281.0
27	55274.0
28	82795.0
29	116299.0
30	157530.0
31	207402.0
32	275576.0
33	378726.0
34	556492.0
35	868121.0
36	1561785.0
37	2899823.0
38	3410983.0
39	1409715.0
40	22624.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.53707474750957	20.389047582267608	14.07656310031429	23.997314569908532
2	14.504429305316924	21.303486725012455	41.96701731378867	22.225066655881946
3	18.250557999641316	26.446514412518283	30.373669975160606	24.929257612679795
4	12.44134297619459	18.14531303621924	39.13688452442427	30.276459463161903
5	13.070316382116905	38.026260700243355	35.789183451638365	13.11423946600138
6	29.5413828030485	38.295014956241786	19.03472032648315	13.12888191422657
7	26.186515235945578	32.69337102195184	23.59911610532084	17.52099763678174
8	23.894029434647518	37.442962373149804	21.50983591395618	17.153172278246494
9	25.51269637269699	16.975185048045034	21.515678489608835	35.99644008964915
10-11	23.998664246154952	27.01976933094089	29.196642847325315	19.78492357557884
12-13	24.768025464294034	24.551487305995888	29.32338217213501	21.35710505757507
14-15	21.904294573705727	25.906703889849698	26.8750049983448	25.313996538099776
16-17	21.487803195312345	28.9856842233499	27.581765591044523	21.944746990293236
18-19	21.579511736367074	28.297330083811538	29.100398322774705	21.022759857046676
20-21	22.39952701042944	27.52215108151446	28.86006712288215	21.218254785173958
22-23	22.069989781748305	27.470018981489332	28.757588316887723	21.702402919874643
24-25	21.81859466619435	27.604261636044548	28.867257384746285	21.709886313014813
26-27	21.79486031206741	27.749581113675347	28.74991582466452	21.705642749592723
28-29	21.697051503460205	27.84204310917877	28.771845671364087	21.689059715996937
30-31	21.992095313078732	27.698759262227	28.6973807755301	21.611764649164165
32-33	21.76625378394303	27.86418707181478	28.612435475826807	21.75712366841538
34-35	21.825884160189858	27.88922662620013	28.643692633293412	21.641196580316603
36-37	21.868135627986394	27.81759226063183	28.617479204904793	21.69679290647698
38-39	21.708398239614795	27.942973518237164	28.68067458664367	21.667953655504377
40-41	21.968169658392966	27.80937464320548	28.526634642795358	21.6958210556062
42-43	21.86910512828511	27.893564112399034	28.382374835275183	21.854955924040677
44-45	21.91281591202509	27.990348541156802	28.25941647145337	21.837419075364743
46-47	21.988782393570297	27.938884166066686	28.006536531304764	22.065796909058246
48-49	21.942851202029043	28.022510370279115	28.012373384982748	22.022265042709098
50-51	21.90572090455808	28.124684409132456	27.896798392861992	22.072796293447475
52-53	22.11524576732552	28.057588497883767	27.770851339760487	22.05631439503023
54-55	22.08957732900261	27.968247229236066	27.800212223614412	22.141963218146913
56-57	22.083745128787687	28.064066618051903	27.785832466748083	22.066355786412327
58-59	22.03850159747974	28.131251310901728	27.810904281525616	22.01934281009292
60-61	22.14248379115365	28.11173129831765	27.723766762936158	22.02201814759254
62-63	22.11644238111698	28.125661534041726	27.685495476232397	22.0724006086089
64-65	22.074130821117226	28.094702055699162	27.69932540756025	22.131841715623363
66-67	22.17903344203335	28.105326538628773	27.556368415945993	22.159271603391883
68-69	22.10924047270959	28.095882928308814	27.666348448400097	22.1285281505815
70-71	22.10901653673899	27.884873342737738	27.71466864709742	22.291441473425852
72-73	22.17824454459652	27.90665477349347	27.687823541077115	22.22727714083289
74-75	22.046451694572426	28.048749003489686	27.61217390507746	22.292625396860426
76-77	22.11961358905843	28.00263170277609	27.61223927270493	22.26551543546055
78-79	22.12805082822201	28.006654033883986	27.532711255758148	22.332583882135857
80-81	22.09540984406093	28.040823735306247	27.560871464505976	22.302894956126845
82-83	22.167958356975625	27.97797263496603	27.67421871925977	22.179850288798576
84-85	22.271173560370226	27.945865176933232	27.555625163502185	22.227336099194357
86-87	22.11520253191191	27.990513206562962	27.62371205532976	22.270572206195364
88-89	22.149217549512368	27.977685743126557	27.618320403370124	22.254776303990955
90-91	22.18824301162125	27.99798129079214	27.616472592987023	22.197303104599584
92-93	22.173055096379812	27.989298850412315	27.605592760564573	22.232053292643297
94-95	22.062575774275324	28.01549868458254	27.63523162368997	22.286693917452162
96-97	22.05180921756774	28.03487259553225	27.71479879275408	22.19851939414593
98-99	22.33939137529225	28.601259319854016	27.74119665168594	21.318152653167793
100-101	26.38319072227443	35.686124086724405	28.301134640315173	9.629550550685993
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3486.0
1	2975.5
2	3572.0
3	5020.5
4	6838.5
5	10667.5
6	13772.0
7	15990.5
8	17421.5
9	17057.0
10	15621.5
11	14246.5
12	15281.5
13	19709.0
14	29909.0
15	43531.5
16	54249.0
17	60042.0
18	61174.5
19	59334.0
20	56456.5
21	54281.5
22	55462.0
23	62819.5
24	77192.5
25	97848.0
26	123628.5
27	151009.5
28	177545.5
29	203808.5
30	228420.0
31	252517.0
32	277862.5
33	303117.0
34	329306.0
35	355722.0
36	384576.5
37	410255.0
38	429331.5
39	445439.0
40	462895.5
41	479573.0
42	490909.5
43	502513.0
44	513111.5
45	520465.5
46	524150.5
47	525466.0
48	526233.5
49	520438.5
50	507506.0
51	489768.0
52	466062.0
53	438375.5
54	408059.0
55	376009.0
56	343462.5
57	309144.0
58	274436.5
59	238904.5
60	200444.5
61	162478.5
62	128120.0
63	99242.5
64	74716.0
65	55532.5
66	41769.0
67	31495.5
68	23475.0
69	16707.0
70	11624.0
71	8133.5
72	5450.5
73	3640.0
74	2523.0
75	1780.5
76	1247.5
77	851.0
78	594.0
79	432.0
80	310.0
81	214.5
82	163.5
83	118.0
84	78.0
85	56.5
86	44.5
87	34.5
88	26.0
89	19.0
90	12.0
91	7.5
92	5.5
93	4.0
94	2.5
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0011933771545844412
3	0.0023204555783586356
4	4.392290916178846E-4
5	3.977923848614804E-4
6	5.138151637794123E-4
7	0.0
8	0.0
9	0.0
10-11	1.2431012026921263E-5
12-13	0.0010897853876934308
14-15	8.411651471550054E-4
16-17	1.1187910824229137E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.293129623108772E-5
28-29	2.3236711215314854E-4
30-31	4.5716795506986585E-5
32-33	0.0013215563347781516
34-35	0.002272549783279106
36-37	0.001392976637046344
38-39	8.315401833944903E-4
40-41	6.849593919683974E-5
42-43	6.480547362583557E-5
44-45	7.68547507655017E-4
46-47	1.7681111604414125E-5
48-49	0.0
50-51	0.0
52-53	0.0011294137615546
54-55	0.004126194650021299
56-57	0.006705733130793713
58-59	0.004973850407004694
60-61	2.487866031476194E-4
62-63	3.8718026774483466E-5
64-65	1.419399173224454E-4
66-67	2.4764147497441743E-4
68-69	0.0
70-71	1.6760901023374906E-4
72-73	1.5428756661815695E-5
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	5.5518564769595454E-6
86-87	3.375521349272395E-5
88-89	3.080998421501809E-4
90-91	1.1006494700807269E-4
92-93	5.827965865074113E-4
94-95	3.47422347810742E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	221.0
20-21	829.0
22-23	1835.0
24-25	4176.0
26-27	6823.0
28-29	13809.0
30-31	35523.0
32-33	43658.0
34-35	58022.0
36-37	89425.0
38-39	106768.0
40-41	104894.0
42-43	117226.0
44-45	136849.0
46-47	137026.0
48-49	128775.0
50-51	117963.0
52-53	112392.0
54-55	119725.0
56-57	124283.0
58-59	124388.0
60-61	121952.0
62-63	115409.0
64-65	118266.0
66-67	124909.0
68-69	125867.0
70-71	124028.0
72-73	116561.0
74-75	117790.0
76-77	115803.0
78-79	123110.0
80-81	124400.0
82-83	118793.0
84-85	117600.0
86-87	122332.0
88-89	130100.0
90-91	137078.0
92-93	141613.0
94-95	164475.0
96-97	239421.0
98-99	976442.0
100-101	7006037.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.76280314215369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.97609649202886	77.16602250945296
2	7.083422219540142	12.14988290774357
3	1.6623398958790239	4.2770078773686295
4	0.6053567445884225	2.0766836526764756
5	0.27381725243180105	1.1741667558616977
6	0.14437547056589975	0.7429227036419438
7	0.08475020904403917	0.5087890846150214
8	0.045050693812822507	0.309093902790923
9	0.030394555317399555	0.23460500378514945
>10	0.09236681106060622	1.173922178451965
>50	0.0015998807549239367	0.09026468286431147
>100	3.701617378355075E-4	0.053809687578263835
>500	4.471238987255841E-5	0.025755794502742284
>1k	1.4900848341076999E-5	0.017073258666303034
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.3149365405123366E-5
2	0.0	4.143670675640421E-5	0.0	0.0	4.143670675640421E-5
3	0.0	4.143670675640421E-5	0.0	0.0	4.143670675640421E-5
4	0.0	8.287341351280842E-5	0.0	8.287341351280841E-6	4.143670675640421E-5
5	0.0	8.287341351280842E-5	0.0	8.287341351280841E-6	4.143670675640421E-5
6	0.0	8.287341351280842E-5	0.0	8.287341351280841E-6	5.8011389458965895E-5
7	0.0	8.287341351280842E-5	0.0	8.287341351280841E-6	6.629873081024673E-5
8	0.0	9.94480962153701E-5	0.0	8.287341351280841E-6	6.629873081024673E-5
9	0.0	1.8232150972817852E-4	0.0	2.4862024053842526E-5	8.287341351280842E-5
10-11	0.0	1.8232150972817852E-4	0.0	2.4862024053842526E-5	9.530442553972968E-5
12-13	0.0	1.8232150972817852E-4	0.0	2.4862024053842526E-5	1.0359176689101053E-4
14-15	0.0	2.4862024053842524E-4	0.0	2.4862024053842526E-5	1.408848029717743E-4
16-17	0.0	2.569075818897061E-4	0.0	4.143670675640421E-5	1.7403416837689768E-4
18-19	0.0	2.6933859391662735E-4	0.0	4.972404810768505E-5	1.8232150972817852E-4
20-21	0.0	2.8591327661918903E-4	0.0	6.629873081024673E-5	2.1132720445766147E-4
22-23	0.0	3.14918971348672E-4	0.0	8.287341351280842E-5	2.403328991871444E-4
24-25	0.0	3.190626420243124E-4	0.0	1.0773543756665095E-4	2.569075818897061E-4
26-27	0.0	3.232063126999528E-4	0.0	1.8646518040381894E-4	2.817696059435486E-4
28-29	0.0	3.232063126999528E-4	0.0	6.754183201293886E-4	2.9005694729482945E-4
30-31	0.0	3.4392466607815493E-4	0.0	0.0023991853211958038	2.9005694729482945E-4
32-33	0.0	3.4806833675379536E-4	0.0	0.006368821828459327	3.522120074294358E-4
34-35	0.0	4.1851073823968247E-4	0.0	0.011204485506931698	3.729303608076379E-4
36-37	0.0	4.889531397255696E-4	0.0	0.017569163664715386	3.8950504351019957E-4
38-39	0.0	4.889531397255696E-4	0.0	0.02715761760814732	4.185107382396825E-4
40-41	0.0	5.303898464819739E-4	0.0	0.03684137597711898	4.2265440891532294E-4
42-43	0.0	5.469645291845355E-4	0.0	0.04534418820353313	4.350854209422442E-4
44-45	0.0	6.132632599947823E-4	0.0	0.05465087254102151	4.516601036448059E-4
46-47	0.0	6.629873081024673E-4	0.0	0.06413573471756244	4.7652212769864837E-4
48-49	0.0	6.629873081024673E-4	0.0	0.07297004059802781	5.22102505130693E-4
50-51	0.0	7.375733802639949E-4	0.0	0.08178362812511498	5.386771878332547E-4
52-53	0.0	7.375733802639949E-4	0.0	0.09029058402220477	5.676828825627376E-4
54-55	0.0	7.375733802639949E-4	0.0	0.09863179309226894	6.00832247967861E-4
56-57	0.0	7.458607216152758E-4	0.0	0.10669123255638956	6.215506013460631E-4
58-59	0.0	7.500043922909162E-4	0.0	0.11379348409443724	6.546999667511865E-4
60-61	0.0	7.707227456691183E-4	0.0	0.12106976980086182	6.671309787781078E-4
62-63	0.0	7.914410990473204E-4	0.0	0.12816787766823384	6.79561990805029E-4
64-65	0.0	8.784581832357693E-4	0.0	0.13444968241250474	6.79561990805029E-4
66-67	0.0	9.116075486408926E-4	0.0	0.14097182005596276	6.878493321563099E-4
68-69	0.0	9.281822313434543E-4	0.0	0.14760169313698746	6.919930028319503E-4
70-71	0.0	9.364695726947352E-4	0.0	0.15414454913382364	7.375733802639949E-4
72-73	0.0	9.364695726947352E-4	0.0	0.1593448558317524	7.790100870203991E-4
74-75	0.0	9.530442553972968E-4	0.0	0.16389046256292994	7.8729742837168E-4
76-77	0.0	9.903372914780604E-4	0.0	0.1678145186927614	7.955847697229608E-4
78-79	0.0	0.0010027683035049818	0.0	0.17149824192340574	7.955847697229608E-4
80-81	0.0	0.0010027683035049818	0.0	0.17298167602528502	8.121594524255225E-4
82-83	0.0	0.0010027683035049818	0.0	0.1735659335905503	8.494524885062863E-4
84-85	0.0	0.0010110556448562626	0.0	0.1741501911558156	8.535961591819267E-4
86-87	0.0	0.0010110556448562626	0.0	0.17438638038432713	8.577398298575672E-4
88-89	0.0	0.0010359176689101052	0.0	0.174456822785813	8.701708418844884E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13325	0.0	46.274773	1
GTATCAA	24170	0.0	35.344685	1
TCAACGC	28515	0.0	29.126858	4
ATCAACG	28580	0.0	29.090652	3
CAACGCA	29010	0.0	28.557901	5
AACGCAG	29875	0.0	27.884476	6
TATCAAC	30370	0.0	27.609213	2
ACGCAGA	35285	0.0	23.50317	7
CGCAGAG	35720	0.0	23.193684	8
GTACATG	38285	0.0	21.065634	1
TACATGG	37085	0.0	20.962986	2
ACATGGG	37400	0.0	20.208296	3
GCAGAGT	43475	0.0	18.970814	9
AGAGTAC	38000	0.0	17.680412	10-11
CATGGGG	24855	0.0	17.652426	4
GAGTACT	22660	0.0	17.382477	12-13
CAGAGTA	41795	0.0	17.15365	10-11
GTGGTAT	7275	0.0	17.13428	1
TGGTATC	6855	0.0	16.062607	2
GTACTTT	26145	0.0	15.113466	14-15
>>END_MODULE