FastQCFastQC Report
Fri 27 May 2016
SRR2138805_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138805_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8261586
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86750.043.537311
GTATCAA148350.033.251591
TCAACGC166900.028.5794584
ATCAACG170450.027.9357683
CAACGCA171300.027.8450325
AACGCAG177000.027.0193846
TATCAAC184000.026.4235152
ACGCAGA206450.023.1044397
CGCAGAG209800.022.755418
GTACATG218800.020.8475671
TACATGG217650.020.152392
ACATGGG219250.019.300583
GCAGAGT259400.018.3080319
CATGGGG164000.017.4814324
GAGTACT144100.017.30371512-13
GTGGTAT47900.017.2509171
CAGAGTA248450.016.95651810-11
AGAGTAC227400.016.66344310-11
TGGTATC47500.015.6389982
GTACTTT164250.015.2447114-15
Produced by FastQC (version 0.11.5)