##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138805_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8261586
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38644480611834	33.0	31.0	34.0	31.0	34.0
2	32.58138631008622	34.0	31.0	34.0	31.0	34.0
3	32.66074625380647	34.0	31.0	34.0	31.0	34.0
4	36.13698507768363	37.0	35.0	37.0	35.0	37.0
5	35.94778472317543	37.0	35.0	37.0	35.0	37.0
6	36.01484073397045	37.0	35.0	37.0	35.0	37.0
7	36.012517330207544	37.0	35.0	37.0	35.0	37.0
8	35.99723709224839	37.0	35.0	37.0	35.0	37.0
9	37.846699047858365	39.0	38.0	39.0	35.0	39.0
10-11	37.71491006690483	39.0	37.0	39.0	35.0	39.0
12-13	37.719954437319906	39.0	37.0	39.0	35.0	39.0
14-15	39.02261666222442	40.0	38.0	41.0	36.0	41.0
16-17	38.988449009669566	40.0	38.0	41.0	36.0	41.0
18-19	39.05483474964734	40.0	38.5	41.0	36.0	41.0
20-21	39.07331128841936	40.0	39.0	41.0	36.0	41.0
22-23	39.03257667346972	40.0	39.0	41.0	36.0	41.0
24-25	38.99979682456882	40.0	38.5	41.0	36.0	41.0
26-27	38.90311975870749	40.0	38.0	41.0	35.0	41.0
28-29	38.83849367682352	40.0	38.0	41.0	35.0	41.0
30-31	38.71010267264934	40.0	38.0	41.0	35.0	41.0
32-33	38.57793938713774	40.0	38.0	41.0	35.0	41.0
34-35	38.53828786329005	40.0	38.0	41.0	35.0	41.0
36-37	38.49269664856875	40.0	38.0	41.0	34.0	41.0
38-39	38.42725229470508	40.0	38.0	41.0	34.0	41.0
40-41	38.34213997448313	40.0	38.0	41.0	34.0	41.0
42-43	38.36399226937499	40.0	38.0	41.0	34.0	41.0
44-45	38.30854170704383	40.0	38.0	41.0	34.0	41.0
46-47	38.26063852338884	40.0	38.0	41.0	34.0	41.0
48-49	38.22056214186293	40.0	38.0	41.0	34.0	41.0
50-51	38.16100801342819	40.0	37.5	41.0	33.5	41.0
52-53	37.910924423009945	40.0	37.0	41.0	33.0	41.0
54-55	37.75888602374578	39.5	37.0	41.0	33.0	41.0
56-57	37.663418468690814	39.0	36.0	41.0	33.0	41.0
58-59	37.52904626034744	39.0	36.0	41.0	32.5	41.0
60-61	37.3419567702697	39.0	36.0	40.0	32.0	41.0
62-63	37.09228928918889	39.0	35.0	40.0	32.0	41.0
64-65	36.84827881949384	38.0	35.0	40.0	31.5	41.0
66-67	36.58409489067558	38.0	35.0	40.0	31.0	41.0
68-69	36.340536982548684	37.0	35.0	40.0	31.0	41.0
70-71	36.03171094182855	37.0	35.0	39.0	31.0	41.0
72-73	35.74186917925965	36.0	34.0	39.0	31.0	40.5
74-75	35.288945617192546	36.0	34.0	39.0	30.5	40.0
76-77	33.33686285934441	34.5	32.0	36.5	27.5	39.0
78-79	34.55925615564735	35.0	34.0	37.0	30.0	39.0
80-81	34.543273060847014	35.0	34.0	37.0	30.0	39.0
82-83	34.280474843007305	35.0	34.0	36.0	30.0	38.5
84-85	34.00048210740027	35.0	34.0	36.0	30.0	37.0
86-87	33.7710186773418	35.0	34.0	35.5	30.0	37.0
88-89	33.63236017571346	35.0	33.0	35.0	30.0	36.5
90-91	33.467643280271304	35.0	33.0	35.0	29.5	36.0
92-93	33.434060504826164	35.0	33.0	35.0	30.0	36.0
94-95	33.30371282619157	35.0	33.0	35.0	29.0	36.0
96-97	33.33017308442689	35.0	33.0	35.0	29.5	35.0
98-99	33.43642022420099	35.0	33.0	35.0	30.0	35.0
100-101	32.195972687178866	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	14.0
20	33.0
21	134.0
22	733.0
23	2357.0
24	6180.0
25	13410.0
26	25057.0
27	40405.0
28	61006.0
29	85500.0
30	115013.0
31	151044.0
32	201590.0
33	277237.0
34	408897.0
35	636722.0
36	1132620.0
37	2005288.0
38	2218857.0
39	865623.0
40	13866.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.4691319560191	20.408381635196925	13.96952110648004	24.15296530230394
2	14.603184132909085	21.266891701739883	41.67439021866055	22.455533946690483
3	18.351298160449637	26.16290829386373	30.32641721079219	25.159376334894446
4	12.638052181491366	18.044216884240853	38.94006572616519	30.377665208102595
5	13.229690907730829	37.87387977988623	35.73373555022268	13.162693762160254
6	29.934991688614705	38.10558031480499	18.907085212367303	13.052342784213003
7	26.396118130344465	32.499776677262695	23.526451216509763	17.57765397588308
8	24.0300349109723	37.396112562406294	21.362314693570944	17.211537833050457
9	25.463125361159467	16.877401022031364	21.39025121810752	36.26922239870166
10-11	24.102764535935624	26.851510524220807	29.196018125092333	19.849706814751237
12-13	24.85592369189279	24.354241644155135	29.265796829452732	21.52403783449933
14-15	21.94333681835857	25.78173251649587	26.751067824844952	25.52386284030061
16-17	21.743162985648983	28.855584904280953	27.415453762200766	21.985798347869302
18-19	21.746932126591673	28.261516614364364	28.848292567553006	21.143258691490956
20-21	22.484076614303792	27.451445467998564	28.681594685036853	21.38288323266079
22-23	22.233380873951226	27.37498253013941	28.513590719283716	21.878045876625652
24-25	21.98483806359087	27.511944530664508	28.57380436184916	21.929413043895465
26-27	21.971913112604216	27.643663953005138	28.485021768118994	21.899401166271655
28-29	21.884297534684837	27.792079929148596	28.476334848270536	21.847287687896028
30-31	22.206028157361153	27.559675135756624	28.38203205720811	21.852264649674105
32-33	22.000078151971255	27.734746853348437	28.332232064122596	21.932942930557715
34-35	22.09949938300932	27.78977702453999	28.343999699964534	21.76672389248615
36-37	22.070964022441792	27.7309860089627	28.317827333431104	21.8802226351644
38-39	21.96385721158015	27.82576980591679	28.3735596773613	21.83681330514176
40-41	22.2208321314696	27.702515728173505	28.19469724727729	21.8819548930796
42-43	22.06081935603146	27.79434644325713	28.044888317353628	22.099945883357783
44-45	22.164167815503507	27.87106445157328	27.914228823139357	22.05053890978386
46-47	22.22642953122253	27.823888970564802	27.723136307044772	22.226545191167897
48-49	22.169093435224173	27.92122492513861	27.76065173198662	22.149029907650597
50-51	22.172121165317137	28.011952280450693	27.64964336159792	22.16628319263425
52-53	22.34470419342572	27.894791547202914	27.530385775426204	22.230118483945166
54-55	22.27261095932027	27.81912800444855	27.58121576791205	22.327045268319132
56-57	22.228546451623	27.924403489809578	27.575464527744998	22.27158553082242
58-59	22.265346020880934	27.969125935533345	27.538127677814266	22.22740036577146
60-61	22.379854818778394	27.882882723744967	27.541580570479585	22.195681886997054
62-63	22.33824547817608	27.952247707087107	27.47924675947422	22.230260055262594
64-65	22.30674477048595	27.917583859752426	27.474937962238588	22.30073340752304
66-67	22.395679117659657	27.89398313022883	27.347541265876025	22.362796486235492
68-69	22.268876921676164	27.95339639897213	27.426948903958092	22.350777775393617
70-71	22.297198070128868	27.73589044658892	27.48333593016602	22.48357555311619
72-73	22.34724543952834	27.764124569467004	27.418011590445758	22.4706184005589
74-75	22.245776636177023	27.872539363343517	27.40381730397436	22.477866696505096
76-77	22.32601503425155	27.819142915364147	27.378877282291814	22.47596476809249
78-79	22.327509466449115	27.829071343846557	27.346426950150178	22.49699223955415
80-81	22.32423855405107	27.827450440126523	27.370669170853002	22.477641834969404
82-83	22.394044820099587	27.753241303944655	27.43196243849622	22.420751437459536
84-85	22.48243696829303	27.795023484209068	27.33553274235444	22.38700680514346
86-87	22.312371840354224	27.807898088343702	27.42532300497643	22.454407066325636
88-89	22.359568521928505	27.782804139223078	27.395301211682398	22.46232612716602
90-91	22.380434191196475	27.779849075240552	27.437259079132957	22.402457654430012
92-93	22.396205044534724	27.788878417871203	27.383639823310936	22.43127671428314
94-95	22.297740283958923	27.81777706105098	27.425929200979915	22.45855345401019
96-97	22.241788088307807	27.84236017971925	27.47749350193783	22.438358230035117
98-99	22.54638108747587	28.39946963213421	27.543733590577702	21.510415689812223
100-101	26.695269197827407	35.46818161961441	28.02263812799799	9.813911054560185
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1559.0
1	1426.0
2	1936.5
3	2757.5
4	3852.0
5	6371.5
6	8455.0
7	9663.0
8	10264.0
9	9901.0
10	9044.5
11	8291.5
12	8984.5
13	11634.5
14	17781.5
15	26067.5
16	32553.0
17	36323.0
18	37602.0
19	36862.5
20	35473.0
21	34616.5
22	35846.5
23	40658.0
24	49505.0
25	62526.0
26	79059.0
27	97007.0
28	115711.5
29	135617.5
30	153512.0
31	169520.0
32	187510.0
33	205721.5
34	222047.5
35	238341.5
36	256761.5
37	273716.0
38	287620.0
39	299338.5
40	310545.0
41	321657.0
42	329624.0
43	336918.0
44	344139.5
45	350715.5
46	355130.0
47	357415.0
48	358515.5
49	353848.5
50	345676.5
51	336065.5
52	321723.5
53	305464.5
54	288343.0
55	267856.5
56	245808.5
57	222253.5
58	196815.5
59	170420.5
60	142670.5
61	115874.5
62	92078.5
63	71645.0
64	53755.0
65	39806.0
66	29909.5
67	22600.0
68	17054.5
69	12086.5
70	8314.0
71	5805.5
72	3948.0
73	2567.5
74	1688.5
75	1178.5
76	833.0
77	578.0
78	415.5
79	297.0
80	215.5
81	159.5
82	116.0
83	75.5
84	50.5
85	33.0
86	23.5
87	20.0
88	15.5
89	10.0
90	6.0
91	3.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	7.141485908395797E-4
3	0.001997195211670011
4	4.357516825461842E-4
5	4.8416853616242695E-4
6	3.2681376190963815E-4
7	0.0
8	0.0
9	0.0
10-11	6.0521067020303365E-6
12-13	9.501807522187628E-4
14-15	5.870543500969427E-4
16-17	1.452505608487281E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.0281435051742194E-5
28-29	2.0604751892122245E-4
30-31	4.248461950562348E-5
32-33	0.001150354567884523
34-35	0.0017713506389811483
36-37	0.0013076081183187671
38-39	6.865185143219822E-4
40-41	1.8710091375096917E-5
42-43	5.032348249066767E-5
44-45	6.226202643455044E-4
46-47	3.212775228012265E-5
48-49	0.0
50-51	0.0
52-53	8.622873392140887E-4
54-55	0.0034908031741786158
56-57	0.005885860632031707
58-59	0.004314933429823464
60-61	2.0781496488031E-4
62-63	4.2025832859200224E-5
64-65	9.20466979804813E-5
66-67	1.3608563424894374E-4
68-69	0.0
70-71	1.2476523952357292E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	2.9677198637321963E-4
90-91	1.0881973032294431E-4
92-93	4.0840205548754526E-4
94-95	2.858045801656298E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	131.0
20-21	551.0
22-23	1242.0
24-25	2881.0
26-27	4426.0
28-29	8862.0
30-31	22430.0
32-33	27213.0
34-35	36295.0
36-37	56356.0
38-39	67585.0
40-41	67312.0
42-43	75233.0
44-45	87346.0
46-47	87276.0
48-49	82774.0
50-51	77181.0
52-53	74353.0
54-55	79715.0
56-57	81582.0
58-59	82170.0
60-61	80858.0
62-63	76958.0
64-65	79208.0
66-67	83195.0
68-69	84495.0
70-71	84167.0
72-73	80645.0
74-75	81144.0
76-77	79628.0
78-79	84761.0
80-81	85992.0
82-83	82809.0
84-85	81862.0
86-87	85386.0
88-89	90692.0
90-91	95919.0
92-93	99904.0
94-95	117403.0
96-97	171364.0
98-99	683301.0
100-101	4828981.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.22685842667003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.4154530879857	77.96240473000968
2	6.760096627079433	11.658037896275756
3	1.637436852949167	4.235731071055798
4	0.553776493950975	1.910016293757136
5	0.2545861460137183	1.0976081784858216
6	0.13384382630006936	0.6924559596995937
7	0.06773781573632583	0.40885733333396196
8	0.0485976508634841	0.33523382086995057
9	0.029526697732046497	0.2291394946633427
>10	0.09676036194141026	1.304554472001806
>50	0.001786785506558393	0.10125005320603413
>100	3.7591245672228266E-4	0.05408114818637031
>500	2.174148447529994E-5	0.010629548454757673
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2104213404060673E-5	0.0
2	0.0	0.0	0.0	2.4208426808121346E-5	0.0
3	0.0	0.0	0.0	3.631264021218202E-5	0.0
4	0.0	2.4208426808121346E-5	0.0	3.631264021218202E-5	0.0
5	0.0	2.4208426808121346E-5	0.0	3.631264021218202E-5	0.0
6	0.0	2.4208426808121346E-5	0.0	3.631264021218202E-5	2.4208426808121346E-5
7	0.0	2.4208426808121346E-5	0.0	6.052106702030337E-5	3.631264021218202E-5
8	0.0	2.4208426808121346E-5	0.0	7.262528042436404E-5	3.631264021218202E-5
9	0.0	6.052106702030337E-5	0.0	1.2104213404060674E-4	3.631264021218202E-5
10-11	6.0521067020303365E-6	6.052106702030337E-5	0.0	1.4525056084872806E-4	3.631264021218202E-5
12-13	1.2104213404060673E-5	6.052106702030337E-5	0.0	1.5735477425278874E-4	4.2364746914212354E-5
14-15	1.2104213404060673E-5	1.2104213404060674E-4	0.0	1.6340688095481908E-4	5.446896031827303E-5
16-17	1.2104213404060673E-5	1.331463474446674E-4	0.0	1.815632010609101E-4	6.052106702030337E-5
18-19	1.2104213404060673E-5	1.6340688095481908E-4	0.0	2.2392794797512246E-4	6.052106702030337E-5
20-21	1.2104213404060673E-5	1.9366741446497077E-4	0.0	2.5418848148527415E-4	6.052106702030337E-5
22-23	1.2104213404060673E-5	1.9366741446497077E-4	0.0	2.783969082933955E-4	9.078160053045505E-5
24-25	1.2104213404060673E-5	2.3603216137918313E-4	0.0	2.783969082933955E-4	1.331463474446674E-4
26-27	1.2104213404060673E-5	2.5418848148527415E-4	0.0	3.631264021218202E-4	1.452505608487281E-4
28-29	1.2104213404060673E-5	2.602405881873045E-4	0.0	7.141485908395797E-4	1.452505608487281E-4
30-31	1.2104213404060673E-5	3.3891797531369883E-4	0.0	0.0019306220379476772	1.513026675507584E-4
32-33	1.2104213404060673E-5	3.3891797531369883E-4	0.0	0.004393829465674024	1.815632010609101E-4
34-35	1.2104213404060673E-5	3.812827222279112E-4	0.0	0.0077890613255130435	1.9366741446497077E-4
36-37	1.2104213404060673E-5	4.2364746914212356E-4	0.0	0.01260048615362716	1.9366741446497077E-4
38-39	2.4208426808121346E-5	4.599601093543056E-4	0.0	0.019923535263083866	1.9366741446497077E-4
40-41	2.4208426808121346E-5	5.20481176374609E-4	0.0	0.02768233605508676	1.997195211670011E-4
42-43	2.4208426808121346E-5	5.507417098847607E-4	0.0	0.034484903988168863	2.1182373457106178E-4
44-45	2.4208426808121346E-5	5.810022433949124E-4	0.0	0.04180795309762556	2.1787584127309212E-4
46-47	2.4208426808121346E-5	5.810022433949124E-4	0.0	0.04979068183760358	2.4208426808121347E-4
48-49	2.4208426808121346E-5	5.810022433949124E-4	0.0	0.05757369105641459	2.4208426808121347E-4
50-51	2.4208426808121346E-5	6.052106702030336E-4	0.0	0.06524776235458907	2.4208426808121347E-4
52-53	2.4208426808121346E-5	6.052106702030336E-4	0.0	0.07234688351607065	2.5418848148527415E-4
54-55	2.4208426808121346E-5	6.112627769050639E-4	0.0	0.0797788705461639	2.7234480159136516E-4
56-57	2.4208426808121346E-5	6.173148836070943E-4	0.0	0.08707771122881248	2.783969082933955E-4
58-59	2.4208426808121346E-5	6.173148836070943E-4	0.0	0.09412841553667782	2.9655322839948646E-4
60-61	2.4208426808121346E-5	6.29419097011155E-4	0.0	0.10125779723166956	3.147095485055775E-4
62-63	2.4208426808121346E-5	6.415233104152156E-4	0.0	0.10854453370091408	3.2681376190963815E-4
64-65	2.4208426808121346E-5	6.899401640314583E-4	0.0	0.11569207171601192	3.2681376190963815E-4
66-67	2.4208426808121346E-5	6.899401640314583E-4	0.0	0.12244017068877575	3.328658686116685E-4
68-69	2.4208426808121346E-5	7.323049109456707E-4	0.0	0.12949087499664108	3.3891797531369883E-4
70-71	3.631264021218202E-5	7.504612310517617E-4	0.0	0.13605135866164197	3.510221887177595E-4
72-73	3.631264021218202E-5	7.504612310517617E-4	0.0	0.14137721255942864	3.994390423340022E-4
74-75	3.631264021218202E-5	7.746696578598831E-4	0.0	0.14548054090340523	3.994390423340022E-4
76-77	3.631264021218202E-5	8.049301913700348E-4	0.0	0.14925100337877012	4.0549114903603255E-4
78-79	3.631264021218202E-5	8.109822980720651E-4	0.0	0.15260387049169494	4.115432557380629E-4
80-81	3.631264021218202E-5	8.109822980720651E-4	0.0	0.1541290013806066	4.115432557380629E-4
82-83	3.631264021218202E-5	8.170344047740954E-4	0.0	0.1546676388770873	4.115432557380629E-4
84-85	3.631264021218202E-5	8.230865114761258E-4	0.0	0.15510339055963346	4.115432557380629E-4
86-87	3.631264021218202E-5	8.230865114761258E-4	0.0	0.15531521429420453	4.2364746914212356E-4
88-89	4.841685361624269E-5	8.351907248801865E-4	0.0	0.15540599589473497	4.2364746914212356E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8675	0.0	43.53731	1
GTATCAA	14835	0.0	33.25159	1
TCAACGC	16690	0.0	28.579458	4
ATCAACG	17045	0.0	27.935768	3
CAACGCA	17130	0.0	27.845032	5
AACGCAG	17700	0.0	27.019384	6
TATCAAC	18400	0.0	26.423515	2
ACGCAGA	20645	0.0	23.104439	7
CGCAGAG	20980	0.0	22.75541	8
GTACATG	21880	0.0	20.847567	1
TACATGG	21765	0.0	20.15239	2
ACATGGG	21925	0.0	19.30058	3
GCAGAGT	25940	0.0	18.308031	9
CATGGGG	16400	0.0	17.481432	4
GAGTACT	14410	0.0	17.303715	12-13
GTGGTAT	4790	0.0	17.250917	1
CAGAGTA	24845	0.0	16.956518	10-11
AGAGTAC	22740	0.0	16.663443	10-11
TGGTATC	4750	0.0	15.638998	2
GTACTTT	16425	0.0	15.24471	14-15
>>END_MODULE