FastQCFastQC Report
Fri 27 May 2016
SRR2138804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9081916
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126400.042.776581
GTATCAA213850.032.9119761
ATCAACG243650.028.02973
TCAACGC243750.027.9659274
CAACGCA247900.027.497765
AACGCAG255150.026.749866
TATCAAC263350.026.2029572
ACGCAGA294950.023.1402847
CGCAGAG297700.022.8844858
TACATGG302250.022.319882
GTACATG308850.022.2616711
ACATGGG304500.021.3459913
GCAGAGT354750.019.1222559
CATGGGG199150.018.6855724
GAGTACT190200.018.41387412-13
CAGAGTA338200.017.5661810-11
AGAGTAC316450.017.37605910-11
GTACTTT221450.015.87204214-15
AGTACTT208100.015.67724112-13
ATGGGGG122750.015.463645
Produced by FastQC (version 0.11.5)