##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138804_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9081916
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43410454357869	33.0	31.0	34.0	31.0	34.0
2	32.63267189434476	34.0	31.0	34.0	31.0	34.0
3	32.71790974503618	34.0	31.0	34.0	31.0	34.0
4	36.18293760920053	37.0	35.0	37.0	35.0	37.0
5	35.99555842621755	37.0	35.0	37.0	35.0	37.0
6	36.05741960176685	37.0	35.0	37.0	35.0	37.0
7	36.06384996293733	37.0	35.0	37.0	35.0	37.0
8	36.05270716003099	37.0	35.0	37.0	35.0	37.0
9	37.92370442536575	39.0	38.0	39.0	35.0	39.0
10-11	37.80368663396578	39.0	38.0	39.0	35.0	39.0
12-13	37.812248703907855	39.0	38.0	39.0	35.0	39.0
14-15	39.12900664353205	40.0	38.5	41.0	36.0	41.0
16-17	39.108039206704845	40.0	38.5	41.0	36.0	41.0
18-19	39.18836818133971	40.0	39.0	41.0	36.0	41.0
20-21	39.21105473642043	40.0	39.0	41.0	36.0	41.0
22-23	39.175862104776655	40.0	39.0	41.0	36.0	41.0
24-25	39.14574385834589	40.0	39.0	41.0	36.0	41.0
26-27	39.05815147656827	40.0	39.0	41.0	36.0	41.0
28-29	38.998585922611	40.0	39.0	41.0	35.5	41.0
30-31	38.866663407995844	40.0	38.0	41.0	35.0	41.0
32-33	38.735350808925475	40.0	38.0	41.0	35.0	41.0
34-35	38.69847318370279	40.0	38.0	41.0	35.0	41.0
36-37	38.653351415431445	40.0	38.0	41.0	35.0	41.0
38-39	38.59114694571185	40.0	38.0	41.0	35.0	41.0
40-41	38.51365623571911	40.0	38.0	41.0	35.0	41.0
42-43	38.53934219207582	40.0	38.0	41.0	35.0	41.0
44-45	38.488414009471455	40.0	38.0	41.0	34.0	41.0
46-47	38.44748762380438	40.0	38.0	41.0	34.0	41.0
48-49	38.41078693052974	40.0	38.0	41.0	34.0	41.0
50-51	38.362194502921895	40.0	38.0	41.0	34.0	41.0
52-53	38.12484822056573	40.0	37.5	41.0	33.5	41.0
54-55	37.99240665534957	40.0	37.0	41.0	33.0	41.0
56-57	37.91116661237761	40.0	37.0	41.0	33.0	41.0
58-59	37.79356183084981	39.5	36.5	41.0	33.0	41.0
60-61	37.627021514996216	39.0	36.0	41.0	33.0	41.0
62-63	37.392660731547394	39.0	36.0	40.5	32.5	41.0
64-65	37.1665610390613	39.0	35.0	40.0	32.0	41.0
66-67	36.91780279562811	38.5	35.0	40.0	32.0	41.0
68-69	36.68594593494328	38.0	35.0	40.0	32.0	41.0
70-71	36.384578352939045	37.0	35.0	40.0	31.0	41.0
72-73	36.09704001186083	37.0	35.0	39.0	31.0	41.0
74-75	35.652202042647346	36.0	34.5	39.0	31.0	40.5
76-77	33.71028242871368	34.5	32.0	36.5	28.0	39.0
78-79	34.879972399596454	35.0	34.0	37.0	30.5	39.0
80-81	34.84120714217656	35.0	34.0	37.0	31.0	39.0
82-83	34.56107871531645	35.0	34.0	36.5	31.0	39.0
84-85	34.267934220773256	35.0	34.0	36.0	30.0	37.5
86-87	34.02254913071058	35.0	34.0	36.0	30.0	37.0
88-89	33.87395937386073	35.0	34.0	35.5	30.0	37.0
90-91	33.69723591935104	35.0	34.0	35.0	30.0	36.0
92-93	33.65320245956761	35.0	34.0	35.0	30.0	36.0
94-95	33.515699111565155	35.0	33.5	35.0	30.0	36.0
96-97	33.527079810673655	35.0	34.0	35.0	30.0	36.0
98-99	33.616824985819946	35.0	34.0	35.0	30.5	35.0
100-101	32.40006146123084	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	8.0
20	35.0
21	158.0
22	624.0
23	2078.0
24	5705.0
25	12387.0
26	23484.0
27	38732.0
28	58929.0
29	83439.0
30	112441.0
31	148584.0
32	198287.0
33	272686.0
34	401467.0
35	622519.0
36	1123668.0
37	2138233.0
38	2678151.0
39	1143786.0
40	16512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.706606843754116	20.607501765046056	14.117131230898854	23.568760160300975
2	14.318262874337673	21.47488930341726	42.28498799853377	21.921859823711294
3	18.124446070180024	26.818202889524773	30.58040558701503	24.476945453280173
4	12.15492797079721	18.327895394253517	39.80415050993892	29.71302612501035
5	12.754041508136748	38.38850802069439	35.9887961548058	12.868654316363063
6	28.95680363836723	38.8332652857185	19.344395365010488	12.865535710903783
7	26.007573732238882	32.98074987700833	23.826073705152083	17.185602685600703
8	23.808555375319482	37.566753535267225	21.62934561385505	16.995345475558242
9	25.48214495707734	17.01365658964474	21.715902239131037	35.78829621414688
10-11	23.68568613086083	27.257432641825396	29.51821672421418	19.538664503099593
12-13	24.5761978420213	24.8382595053509	29.6470359724778	20.938506680149995
14-15	21.692057328190696	26.234760882130892	27.301426873457878	24.771754916220534
16-17	21.130914154121445	29.388191987599082	27.977717681507396	21.503176176772076
18-19	21.133095703593824	28.646053321788045	29.637716314486944	20.58313466013119
20-21	22.07263769425497	27.792167493464344	29.423215232200278	20.711979580080413
22-23	21.718308621409076	27.718615430376666	29.2930982628952	21.26997768531906
24-25	21.44032548528668	27.82860869348272	29.39553590060437	21.335529920626225
26-27	21.428292421059624	27.971712746678946	29.30552370099553	21.294471131265905
28-29	21.330223134288534	28.12098793364497	29.31620606876927	21.232582863297225
30-31	21.574455428696776	27.98407980613613	29.221871731982795	21.219593033184296
32-33	21.3901386579762	28.149139888253544	29.137874055720197	21.322847398050058
34-35	21.478908100353962	28.185024909592656	29.14896808671994	21.187098903333446
36-37	21.475571742491837	28.08575773026315	29.149696879305033	21.28897364793998
38-39	21.37036791823507	28.20161394795561	29.163053699469344	21.26496443433998
40-41	21.574878825892498	28.087072470318216	29.046431299731978	21.291617404057313
42-43	21.487053780739995	28.200957110258194	28.86996739737697	21.442021711624836
44-45	21.55570645184522	28.34246745258356	28.673163838575032	21.428662256996187
46-47	21.625915584825	28.276017687220772	28.472665836995752	21.62540089095848
48-49	21.605301728965898	28.345738033350475	28.430114489857637	21.61884574782599
50-51	21.592975339362102	28.46467132011688	28.29820432689748	21.64414901362354
52-53	21.752998850822326	28.394722341701563	28.17300455198149	21.67927425549462
54-55	21.739103356117774	28.320231440817395	28.229673748679968	21.710991454384867
56-57	21.708759322300907	28.410784778579977	28.196654197321752	21.68380170179736
58-59	21.71174777182382	28.440495507248357	28.168056551124852	21.679700169802967
60-61	21.776650989954266	28.387405719017256	28.178007496884504	21.657935794143974
62-63	21.770293849545585	28.395479630720505	28.110553967136486	21.723672552597424
64-65	21.72193848897262	28.39263069911993	28.138458555106176	21.74697225680127
66-67	21.83045417053658	28.365955530009725	27.990310345937708	21.81327995351599
68-69	21.782479470215485	28.372925009769794	28.061554187578956	21.783041332435765
70-71	21.750817723068025	28.194147472656656	28.1337236624889	21.92131114178642
72-73	21.833983497012987	28.209268113077506	28.13000399641895	21.826744393490554
74-75	21.755347192547237	28.287542598186814	28.064558226235793	21.892551983030163
76-77	21.800645110512324	28.268707082968312	28.029095202392607	21.90155260412676
78-79	21.78349214009419	28.25120745079183	27.99880969675055	21.96649071236343
80-81	21.761369644398677	28.25039168175128	28.03494443655761	21.953294237292436
82-83	21.87684770367929	28.18887814299365	28.09295295755468	21.84132119577238
84-85	21.981221913827184	28.164316567566893	28.005003954248934	21.849457564356985
86-87	21.79340988096899	28.183600882804487	28.108608383168093	21.91438085305843
88-89	21.861503634328876	28.136813056334674	28.0646378734659	21.937045435870548
90-91	21.895232233044112	28.098464050532552	28.137311468005503	21.868992248417836
92-93	21.90572605555509	28.027530172385628	28.131550885669633	21.935192886389654
94-95	21.781462687713514	28.082017311352146	28.146312320927112	21.99020768000722
96-97	21.832391666713143	28.081435390742143	28.193363628641055	21.892809313903662
98-99	22.050186650169486	28.63400641702331	28.272639359803435	21.043167573003768
100-101	25.94006086828048	35.636883351546494	28.99038852550276	9.432667254670267
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1392.0
1	1323.5
2	2138.0
3	3297.5
4	4760.0
5	8005.5
6	10666.0
7	12500.5
8	13658.5
9	13160.0
10	11816.0
11	10672.0
12	11671.0
13	15832.5
14	24889.0
15	36476.5
16	45291.5
17	50156.5
18	51079.5
19	48861.5
20	45610.5
21	43422.5
22	44420.5
23	50496.0
24	61975.0
25	78500.0
26	99289.0
27	121636.5
28	144922.5
29	168362.5
30	188968.0
31	209009.5
32	229681.0
33	248975.5
34	268685.0
35	287966.5
36	307889.0
37	325325.5
38	338293.5
39	349229.5
40	359729.5
41	368691.5
42	374299.0
43	379685.5
44	384981.0
45	389078.0
46	389260.5
47	386656.5
48	382062.0
49	372929.5
50	361157.0
51	345508.0
52	325632.0
53	305083.5
54	283891.0
55	260028.5
56	235768.5
57	211259.0
58	184837.5
59	158473.0
60	132603.0
61	107860.5
62	84550.5
63	64914.0
64	48777.5
65	35828.5
66	26676.5
67	20057.0
68	14912.5
69	10532.0
70	7098.5
71	4788.0
72	3180.0
73	2063.5
74	1382.5
75	957.5
76	684.0
77	464.0
78	313.0
79	230.5
80	177.5
81	128.5
82	94.0
83	66.0
84	44.0
85	30.5
86	26.5
87	24.5
88	20.5
89	18.0
90	14.5
91	11.5
92	8.5
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001200187273258198
3	0.0023673418692707576
4	4.844792662693643E-4
5	3.74370342117236E-4
6	4.40435696608513E-4
7	0.0
8	0.0
9	0.0
10-11	5.505446207606412E-6
12-13	9.249149628778772E-4
14-15	7.322243456116529E-4
16-17	7.157080069888336E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.958382095348365E-5
28-29	2.1500549835214825E-4
30-31	4.4169369878112964E-5
32-33	0.0013398277701229813
34-35	0.001961171798482875
36-37	0.0014071298487698361
38-39	7.818854577179969E-4
40-41	4.5377167071695694E-5
42-43	4.577009122494095E-5
44-45	6.993739793691612E-4
46-47	2.924396113664726E-5
48-49	0.0
50-51	0.0
52-53	0.0010154633305311381
54-55	0.003687871950735892
56-57	0.006567688392814431
58-59	0.004694850042931032
60-61	2.715431436903383E-4
62-63	5.1084808252852946E-5
64-65	1.1619579611356049E-4
66-67	2.2855369653606628E-4
68-69	0.0
70-71	1.3388816589279308E-4
72-73	6.7781863593201996E-6
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	1.4848527571748272E-5
88-89	3.2385712327508425E-4
90-91	1.3005223280175885E-4
92-93	6.378734729736899E-4
94-95	2.8505095404574796E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	214.0
20-21	783.0
22-23	1504.0
24-25	2952.0
26-27	4909.0
28-29	9911.0
30-31	23993.0
32-33	29049.0
34-35	40171.0
36-37	61167.0
38-39	74074.0
40-41	74080.0
42-43	84220.0
44-45	100640.0
46-47	99932.0
48-49	94304.0
50-51	87442.0
52-53	82126.0
54-55	87485.0
56-57	91457.0
58-59	90987.0
60-61	89142.0
62-63	84458.0
64-65	87401.0
66-67	91778.0
68-69	95256.0
70-71	93419.0
72-73	89670.0
74-75	90089.0
76-77	88462.0
78-79	94368.0
80-81	96009.0
82-83	91587.0
84-85	90992.0
86-87	94616.0
88-89	100795.0
90-91	108105.0
92-93	109279.0
94-95	124424.0
96-97	178107.0
98-99	732205.0
100-101	5310354.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.31513761361866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.31705008262301	77.95728606737693
2	6.84503346484507	11.816600109758541
3	1.6228873421329737	4.202392328028223
4	0.5977170593545186	2.0636812092877097
5	0.2517972357562249	1.0866956527513667
6	0.13130739984563136	0.6800289772417271
7	0.07698019715271183	0.4651189417531872
8	0.04242697738744588	0.2929672313381822
9	0.03050069075347611	0.23694041877270444
>10	0.08274468329449818	1.0504262529459556
>50	0.001288797633128582	0.07207852346385088
>100	2.2769604849745794E-4	0.045444686165189534
>500	1.9186643822617234E-5	0.01185766026155168
>1k	1.9186528991427863E-5	0.018481940854886223
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1010892415212825E-5
2	0.0	2.202178483042565E-5	0.0	0.0	1.1010892415212825E-5
3	0.0	2.202178483042565E-5	0.0	0.0	1.1010892415212825E-5
4	0.0	8.80871393217026E-5	0.0	0.0	1.1010892415212825E-5
5	0.0	8.80871393217026E-5	0.0	0.0	1.1010892415212825E-5
6	3.3032677245638474E-5	8.80871393217026E-5	0.0	0.0	2.202178483042565E-5
7	5.505446207606412E-5	8.80871393217026E-5	0.0	2.202178483042565E-5	2.202178483042565E-5
8	5.505446207606412E-5	1.321307089825539E-4	0.0	2.202178483042565E-5	2.202178483042565E-5
9	8.80871393217026E-5	2.8628320279553343E-4	0.0	4.40435696608513E-5	2.202178483042565E-5
10-11	1.321307089825539E-4	2.8628320279553343E-4	0.0	7.157080069888336E-5	2.202178483042565E-5
12-13	1.5415249381297956E-4	2.917886490031398E-4	0.0	9.909803173691542E-5	2.202178483042565E-5
14-15	2.0920695588904368E-4	4.5144658902372583E-4	0.0	1.1561437035973466E-4	2.202178483042565E-5
16-17	2.53250525549895E-4	4.7346837385415147E-4	0.0	1.321307089825539E-4	3.3032677245638474E-5
18-19	2.6426141796510785E-4	4.954901586845771E-4	0.0	1.816797248510116E-4	4.40435696608513E-5
20-21	2.7527231038032064E-4	5.175119435150028E-4	0.0	2.4774507934228855E-4	4.40435696608513E-5
22-23	2.7527231038032064E-4	5.835772980062796E-4	0.0	3.46843111079204E-4	6.055990828367054E-5
24-25	3.0279954141835267E-4	6.27620867667131E-4	0.0	4.5144658902372583E-4	7.707624690648978E-5
26-27	3.248213262487783E-4	6.606535449127695E-4	0.0	5.890827442138862E-4	7.707624690648978E-5
28-29	3.358322186639912E-4	6.606535449127695E-4	0.0	0.001602084846413466	7.707624690648978E-5
30-31	3.6335944970202323E-4	7.377297918192593E-4	0.0	0.003325289509394273	7.707624690648978E-5
32-33	3.743703421172361E-4	7.487406842344722E-4	0.0	0.006606535449127695	9.909803173691543E-5
34-35	4.0740301936287456E-4	9.028931780474517E-4	0.0	0.010493380471697823	9.909803173691543E-5
36-37	4.40435696608513E-4	0.0010350238870300056	0.0	0.015954783109643385	9.909803173691543E-5
38-39	4.5144658902372583E-4	0.0010515402256528247	0.0	0.02397071278791832	9.909803173691543E-5
40-41	4.624574814389387E-4	0.0012001872732581979	0.0	0.03308222626150693	1.1010892415212825E-4
42-43	4.6796292764654507E-4	0.0012222090580886235	0.0	0.04141196637361544	1.1010892415212825E-4
44-45	4.789738200617579E-4	0.0013378234284483583	0.0	0.05096391554381256	1.1010892415212825E-4
46-47	4.844792662693643E-4	0.0014369214601852739	0.0	0.06086821327129649	1.1010892415212825E-4
48-49	4.844792662693643E-4	0.0014424269063928801	0.0	0.07045870056494688	1.1010892415212825E-4
50-51	4.954901586845771E-4	0.001574557615375434	0.0	0.08043456909312968	1.1010892415212825E-4
52-53	5.0650105109979E-4	0.001574557615375434	0.0	0.08974427863019213	1.1561437035973466E-4
54-55	5.175119435150028E-4	0.0015855685077906469	0.0	0.0987291668410058	1.2662526277494747E-4
56-57	5.285228359302156E-4	0.001629612077451498	0.0	0.10727912480141855	1.321307089825539E-4
58-59	5.285228359302156E-4	0.0016351175236591045	0.0	0.11461237914995029	1.321307089825539E-4
60-61	5.395337283454285E-4	0.0016681502009047431	0.0	0.121736426542593	1.321307089825539E-4
62-63	5.395337283454285E-4	0.0017452264478112329	0.0	0.1285246417165717	1.4314160139776671E-4
64-65	5.395337283454285E-4	0.0018718517105861803	0.0	0.13510364993466137	1.4314160139776671E-4
66-67	5.560500669682477E-4	0.0018938734954166059	0.0	0.14219466465005842	1.4314160139776671E-4
68-69	5.615555131758541E-4	0.0019048843878318187	0.0	0.14925815213441745	1.4314160139776671E-4
70-71	5.615555131758541E-4	0.0019379170650774573	0.0	0.1557490732131854	1.5415249381297956E-4
72-73	6.055990828367053E-4	0.001965444296115489	0.0	0.16124901397458422	1.761742786434052E-4
74-75	6.055990828367053E-4	0.001992971527153521	0.0	0.16595617048208772	1.761742786434052E-4
76-77	6.055990828367053E-4	0.002081058666475224	0.0	0.16992559719777192	1.8167972485101162E-4
78-79	6.055990828367053E-4	0.0021251022361360753	0.0	0.17329493027682705	1.8718517105861804E-4
80-81	6.055990828367053E-4	0.0021251022361360753	0.0	0.1746217428128602	1.9819606347383086E-4
82-83	6.055990828367053E-4	0.0021306076823436816	0.0	0.1751447602025828	2.202178483042565E-4
84-85	6.055990828367053E-4	0.002136113128551288	0.0	0.17568429393092821	2.202178483042565E-4
86-87	6.111045290443117E-4	0.002180156698212139	0.0	0.17595406079510095	2.202178483042565E-4
88-89	6.166099752519182E-4	0.0023012765147794804	0.0	0.17603113704200743	2.202178483042565E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12640	0.0	42.77658	1
GTATCAA	21385	0.0	32.911976	1
ATCAACG	24365	0.0	28.0297	3
TCAACGC	24375	0.0	27.965927	4
CAACGCA	24790	0.0	27.49776	5
AACGCAG	25515	0.0	26.74986	6
TATCAAC	26335	0.0	26.202957	2
ACGCAGA	29495	0.0	23.140284	7
CGCAGAG	29770	0.0	22.884485	8
TACATGG	30225	0.0	22.31988	2
GTACATG	30885	0.0	22.261671	1
ACATGGG	30450	0.0	21.345991	3
GCAGAGT	35475	0.0	19.122255	9
CATGGGG	19915	0.0	18.685572	4
GAGTACT	19020	0.0	18.413874	12-13
CAGAGTA	33820	0.0	17.56618	10-11
AGAGTAC	31645	0.0	17.376059	10-11
GTACTTT	22145	0.0	15.872042	14-15
AGTACTT	20810	0.0	15.677241	12-13
ATGGGGG	12275	0.0	15.46364	5
>>END_MODULE