FastQCFastQC Report
Fri 27 May 2016
SRR2138803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5266359
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62450.043.5381051
GTATCAA103950.034.4162751
TCAACGC124950.027.755434
ATCAACG125300.027.6784293
CAACGCA127100.027.2859295
AACGCAG130650.026.7697646
TATCAAC132800.026.6543052
ACGCAGA150600.023.0838387
CGCAGAG152750.022.7864788
GTACATG146400.020.1533091
TACATGG144750.019.6851862
GCAGAGT183050.019.0376599
ACATGGG146800.018.9227033
AGAGTAC157750.018.07560510-11
GAGTACT99900.018.07351712-13
CATGGGG109100.017.7455774
CAGAGTA176100.017.27952810-11
GTACTTT115850.015.512914-15
AGTACTT111600.015.34903612-13
GTGGTAT34200.014.5219621
Produced by FastQC (version 0.11.5)