##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138803_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5266359
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44885508185067	33.0	31.0	34.0	31.0	34.0
2	32.6395927432976	34.0	31.0	34.0	31.0	34.0
3	32.71968014334002	34.0	31.0	34.0	31.0	34.0
4	36.17985234200707	37.0	35.0	37.0	35.0	37.0
5	35.99702394006941	37.0	35.0	37.0	35.0	37.0
6	36.06400778982215	37.0	35.0	37.0	35.0	37.0
7	36.06436078512688	37.0	35.0	37.0	35.0	37.0
8	36.05125286749346	37.0	35.0	37.0	35.0	37.0
9	37.90920121472919	39.0	38.0	39.0	35.0	39.0
10-11	37.78656354418679	39.0	37.5	39.0	35.0	39.0
12-13	37.79222580534294	39.0	38.0	39.0	35.0	39.0
14-15	39.11417898020245	40.0	38.5	41.0	36.0	41.0
16-17	39.085932994693295	40.0	38.0	41.0	36.0	41.0
18-19	39.151482646739424	40.0	38.5	41.0	36.0	41.0
20-21	39.16995120849535	40.0	39.0	41.0	36.0	41.0
22-23	39.13111177962283	40.0	39.0	41.0	36.0	41.0
24-25	39.09904887534727	40.0	39.0	41.0	36.0	41.0
26-27	39.007268207758926	40.0	39.0	41.0	35.5	41.0
28-29	38.94087754759285	40.0	38.0	41.0	35.0	41.0
30-31	38.809670824890375	40.0	38.0	41.0	35.0	41.0
32-33	38.67576304270706	40.0	38.0	41.0	35.0	41.0
34-35	38.63888303882098	40.0	38.0	41.0	35.0	41.0
36-37	38.590001080715496	40.0	38.0	41.0	35.0	41.0
38-39	38.51942534126946	40.0	38.0	41.0	34.5	41.0
40-41	38.4309114164529	40.0	38.0	41.0	34.0	41.0
42-43	38.45122992274105	40.0	38.0	41.0	34.0	41.0
44-45	38.392154756067676	40.0	38.0	41.0	34.0	41.0
46-47	38.34001424074174	40.0	38.0	41.0	34.0	41.0
48-49	38.292040887777645	40.0	38.0	41.0	34.0	41.0
50-51	38.22675492398097	40.0	37.5	41.0	34.0	41.0
52-53	37.97219669823768	40.0	37.0	41.0	33.0	41.0
54-55	37.81797069852986	40.0	37.0	41.0	33.0	41.0
56-57	37.714762789641725	39.0	36.5	41.0	33.0	41.0
58-59	37.57104407689856	39.0	36.0	41.0	33.0	41.0
60-61	37.381917960346726	39.0	36.0	40.5	32.5	41.0
62-63	37.12148791472841	39.0	35.0	40.0	32.0	41.0
64-65	36.872035141690176	38.0	35.0	40.0	32.0	41.0
66-67	36.6015204442206	38.0	35.0	40.0	31.0	41.0
68-69	36.35355206824163	37.0	35.0	40.0	31.0	41.0
70-71	36.04316977640051	37.0	35.0	39.0	31.0	41.0
72-73	35.75331839470511	36.0	34.0	39.0	31.0	40.5
74-75	35.30142295712868	36.0	34.0	39.0	30.5	40.0
76-77	33.37905534560748	34.5	32.0	36.5	27.5	39.0
78-79	34.5804628419645	35.0	34.0	37.0	30.0	39.0
80-81	34.565734530633165	35.0	34.0	37.0	30.0	39.0
82-83	34.303326470486695	35.0	34.0	36.0	30.0	38.5
84-85	34.034804074028685	35.0	34.0	36.0	30.0	37.0
86-87	33.81259890793879	35.0	34.0	35.5	30.0	37.0
88-89	33.68248693595144	35.0	34.0	35.0	30.0	36.5
90-91	33.52225828359997	35.0	33.0	35.0	30.0	36.0
92-93	33.49025532628017	35.0	33.0	35.0	30.0	36.0
94-95	33.36479182932306	35.0	33.0	35.0	30.0	36.0
96-97	33.391772894239296	35.0	33.0	35.0	30.0	35.0
98-99	33.49388591432192	35.0	33.0	35.0	30.0	35.0
100-101	32.25382626254307	34.0	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	10.0
20	20.0
21	82.0
22	374.0
23	1388.0
24	3666.0
25	8150.0
26	15403.0
27	25031.0
28	37551.0
29	52423.0
30	70425.0
31	93169.0
32	122466.0
33	168695.0
34	251610.0
35	393887.0
36	710982.0
37	1287475.0
38	1443785.0
39	571052.0
40	8714.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.94419711986973	20.058583169130703	13.918344723555688	24.07887498744389
2	14.744123737376135	20.940904598376665	41.57535202508775	22.73961963915945
3	18.4043209519037	26.04782874787301	30.05028058881401	25.497569711409284
4	12.659802451263172	17.81569232253255	38.65002413438084	30.87448109182344
5	13.320892064779667	37.800550059016274	35.54900705309034	13.32955082311372
6	30.30877117960962	37.808058633654916	18.68681910561194	13.196351081123522
7	26.794660219707772	32.349010008622656	23.233983858677316	17.62234591299226
8	24.40676755990239	37.1310615170747	21.029956370236057	17.43221455278685
9	25.625427358826087	16.81784321957542	21.17064180394842	36.38608761765007
10-11	24.33204800508283	26.749781015688452	28.89629248594712	20.0218784932816
12-13	25.088456207400768	24.212007029974423	29.024706404763943	21.674830357860866
14-15	22.212907272822388	25.582106537091185	26.531677542529415	25.673308647557015
16-17	21.902652556712397	28.663574204007976	27.239191903770976	22.194581335508655
18-19	21.891861151129273	28.02342187458166	28.737530046850207	21.347186927438862
20-21	22.698938980079873	27.23120523283696	28.554434771974858	21.515421015108313
22-23	22.41625892435791	27.145819664114658	28.40219454459307	22.035726866934368
24-25	22.109581955971464	27.288038384388834	28.502551263408886	22.099828396230812
26-27	22.167468917796064	27.378342240654817	28.39874943448468	22.055439407064434
28-29	22.04674435336547	27.54918987203142	28.41358242055772	21.99048335404539
30-31	22.410071264370877	27.337570187132048	28.29161440733394	21.96074414116313
32-33	22.158813383635735	27.550949985524202	28.24900509320877	22.041231537631297
34-35	22.258455789222158	27.552170350758487	28.261262853139606	21.92811100687975
36-37	22.269773838419063	27.510638731912422	28.21392472981701	22.005662699851506
38-39	22.128004210404743	27.619774562745164	28.27764061536864	21.974580611481453
40-41	22.363024144744266	27.48352524748744	28.114570855788845	22.03887975197945
42-43	22.198452181568072	27.568148657342118	27.9641388335731	22.26926032751671
44-45	22.297802454919697	27.660503949540466	27.83869402126855	22.20299957427128
46-47	22.35732969859775	27.633370552415133	27.602850101905563	22.406449647081555
48-49	22.312488587003248	27.757798724627115	27.60405887020391	22.325653818165726
50-51	22.277496410806556	27.837344449057653	27.5098488124361	22.375310327699694
52-53	22.51538223144987	27.747483599221678	27.33276733594719	22.40436683338126
54-55	22.436546717067497	27.663999301203607	27.41224243637536	22.487211545353535
56-57	22.419113245256224	27.732521714607532	27.41149930636531	22.436865733770933
58-59	22.40666493411708	27.80843390492185	27.423163789813227	22.361737371147846
60-61	22.526796946507627	27.714841411457268	27.40730658181223	22.351055060222876
62-63	22.488778525150217	27.788295868894497	27.2826653147762	22.44026029117909
64-65	22.42625542875551	27.759229915488103	27.366042120334672	22.44847253542171
66-67	22.538245665283778	27.778131623845546	27.180202168005597	22.50342054286508
68-69	22.41212436338516	27.771777535520105	27.28512285734871	22.530975243746028
70-71	22.43281485157116	27.551152677786455	27.373203624202525	22.64282884643986
72-73	22.487841770851855	27.616023307364955	27.30757933297711	22.58855558880608
74-75	22.367057650600188	27.76423632753233	27.297644617319083	22.571061404548402
76-77	22.46396299103998	27.70362857023199	27.238369644446138	22.594038794281886
78-79	22.451344915007045	27.695598874337385	27.168719555799548	22.684336654856025
80-81	22.466542399307894	27.696495250810006	27.213394848487404	22.623567501394696
82-83	22.48834003538802	27.686879712210622	27.31796777791687	22.506812474484487
84-85	22.604900165887944	27.65902370152421	27.215799567051235	22.520276565536605
86-87	22.433529537245693	27.685370683849452	27.290883032642103	22.59021674626275
88-89	22.422754145367684	27.723924099804552	27.249668241353557	22.603653513474203
90-91	22.50018573938027	27.706364163842444	27.22863476542466	22.564815331352627
92-93	22.515658772815886	27.672042860562975	27.26973556248223	22.542562804138914
94-95	22.393734349079665	27.703479551499342	27.329211343603887	22.573574755817106
96-97	22.36000716255339	27.73861339576277	27.366073931962788	22.535305509721056
98-99	22.676472307744014	28.279459076116105	27.381878016190665	21.66219059994922
100-101	26.876861843090317	35.350726916819994	27.89573035689517	9.87668088319452
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1161.0
1	1030.5
2	1343.0
3	1908.5
4	2706.0
5	4457.0
6	5894.0
7	6821.5
8	7278.5
9	7017.0
10	6291.0
11	5609.5
12	6084.5
13	8001.0
14	12368.0
15	18203.0
16	22734.5
17	25175.0
18	25573.5
19	24477.5
20	23018.5
21	21941.5
22	22151.5
23	24682.0
24	29773.0
25	37468.0
26	47357.5
27	58114.5
28	68995.0
29	80485.0
30	91479.5
31	101698.5
32	112400.5
33	123565.5
34	134813.5
35	144862.5
36	155400.5
37	166213.0
38	175069.0
39	181390.0
40	187860.0
41	195113.0
42	200869.0
43	206590.0
44	212454.5
45	217893.0
46	221157.5
47	223568.5
48	225810.0
49	223853.0
50	220207.5
51	215868.0
52	207418.5
53	198696.0
54	189590.0
55	177352.0
56	164397.0
57	150316.0
58	134556.0
59	117153.5
60	97787.5
61	79310.0
62	62990.0
63	48514.5
64	36553.0
65	27436.5
66	20519.0
67	15439.5
68	11640.5
69	8426.0
70	5987.5
71	4236.0
72	2946.5
73	1958.0
74	1314.0
75	875.5
76	595.0
77	409.5
78	280.5
79	211.0
80	157.5
81	111.5
82	80.5
83	53.0
84	37.0
85	30.0
86	29.5
87	25.0
88	16.0
89	11.5
90	7.5
91	4.5
92	5.0
93	3.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	8.354918455046456E-4
3	0.002126706515830007
4	2.468498634445544E-4
5	2.8482676551294737E-4
6	6.076304330942877E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	9.96893679295316E-4
14-15	6.361131096455825E-4
16-17	1.1393070620517896E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	2.8501393860666758E-5
28-29	1.5210791143668964E-4
30-31	2.856133961822438E-5
32-33	0.0010977239695760552
34-35	0.0018962405881103991
36-37	0.001173941802123834
38-39	7.557278356652382E-4
40-41	9.763972562065424E-6
42-43	2.952799209358484E-5
44-45	6.855327941005234E-4
46-47	1.0042924463449228E-5
48-49	0.0
50-51	0.0
52-53	0.001117904797985287
54-55	0.0038135609600853907
56-57	0.0062161508686200155
58-59	0.0045426708750709
60-61	2.0476415049604653E-4
62-63	2.1776581420786922E-5
64-65	1.0998555559698344E-4
66-67	2.445422341667518E-4
68-69	0.0
70-71	9.096428969397339E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0
88-89	2.1538609287473663E-4
90-91	1.542469982891437E-4
92-93	6.526893673769145E-4
94-95	3.5837874235342544E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	92.0
20-21	334.0
22-23	758.0
24-25	1690.0
26-27	2882.0
28-29	5651.0
30-31	13155.0
32-33	16123.0
34-35	21847.0
36-37	33247.0
38-39	40011.0
40-41	40014.0
42-43	44994.0
44-45	53368.0
46-47	53544.0
48-49	50684.0
50-51	46487.0
52-53	44337.0
54-55	47398.0
56-57	48690.0
58-59	49288.0
60-61	48226.0
62-63	45983.0
64-65	46943.0
66-67	49520.0
68-69	50936.0
70-71	50586.0
72-73	48585.0
74-75	49159.0
76-77	47704.0
78-79	51359.0
80-81	52210.0
82-83	49813.0
84-85	49385.0
86-87	51378.0
88-89	55554.0
90-91	59133.0
92-93	61303.0
94-95	71895.0
96-97	107343.0
98-99	440297.0
100-101	3164453.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.61520992391961
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.0328590766817	79.75863057978057
2	6.539148125301077	11.458576714437184
3	1.4762840642397401	3.880348145871061
4	0.46998853365578946	1.647125761523486
5	0.19933012375105116	0.873217531830461
6	0.10249262780339577	0.5387947860389207
7	0.0574374489372014	0.352267590228912
8	0.031936723650852775	0.22385141975613393
9	0.020913314272421074	0.1649091978164769
>10	0.06786715625262424	0.8934706333113486
>50	0.0011102263949125035	0.06770629541105781
>100	6.012474235964172E-4	0.12408155139589784
>500	3.133163569258632E-5	0.01701979259848748
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.898845103419649E-5	0.0	0.0	0.0
3	0.0	3.797690206839298E-5	0.0	0.0	0.0
4	0.0	7.595380413678596E-5	0.0	0.0	0.0
5	0.0	7.595380413678596E-5	0.0	0.0	0.0
6	5.696535310258947E-5	7.595380413678596E-5	0.0	0.0	0.0
7	5.696535310258947E-5	7.595380413678596E-5	0.0	3.797690206839298E-5	0.0
8	5.696535310258947E-5	9.494225517098246E-5	0.0	3.797690206839298E-5	0.0
9	5.696535310258947E-5	2.278614124103579E-4	0.0	5.696535310258947E-5	0.0
10-11	5.696535310258947E-5	2.278614124103579E-4	0.0	5.696535310258947E-5	0.0
12-13	5.696535310258947E-5	2.278614124103579E-4	0.0	8.54480296538842E-5	0.0
14-15	7.595380413678596E-5	3.6078056964973336E-4	0.0	1.1393070620517895E-4	9.494225517098245E-6
16-17	9.494225517098246E-5	3.6078056964973336E-4	0.0	1.1393070620517895E-4	1.898845103419649E-5
18-19	9.494225517098246E-5	3.7976902068392985E-4	0.0	1.4241338275647369E-4	3.797690206839298E-5
20-21	9.494225517098246E-5	4.367343737865193E-4	0.0	1.5190760827357193E-4	3.797690206839298E-5
22-23	1.1393070620517896E-4	5.221824034404035E-4	0.0	1.5190760827357193E-4	3.797690206839298E-5
24-25	1.3291915723937544E-4	6.17124658611386E-4	0.0	1.8039028482486665E-4	4.7471127585491224E-5
26-27	1.3291915723937544E-4	6.645957861968772E-4	0.0	2.7533253999584913E-4	5.696535310258947E-5
28-29	1.3291915723937544E-4	6.740900117139754E-4	0.0	6.55101560679779E-4	5.696535310258947E-5
30-31	1.3291915723937544E-4	7.500438158507614E-4	0.0	0.0017659259461802737	5.696535310258947E-5
32-33	1.3291915723937544E-4	7.595380413678597E-4	0.0	0.003968586266147067	5.696535310258947E-5
34-35	1.3291915723937544E-4	9.873994537782176E-4	0.0	0.006579498283349084	5.696535310258947E-5
36-37	1.3291915723937544E-4	0.001234249317222772	0.0	0.010908865119145884	5.696535310258947E-5
38-39	1.3291915723937544E-4	0.0012722262192911649	0.0	0.017773190168007914	5.696535310258947E-5
40-41	1.3291915723937544E-4	0.0013481800234279508	0.0	0.02504576691410517	5.696535310258947E-5
42-43	1.3291915723937544E-4	0.0013861569254963438	0.0	0.03113156547056515	5.696535310258947E-5
44-45	1.4241338275647369E-4	0.0015665472103212105	0.0	0.038299705735974326	5.696535310258947E-5
46-47	1.5190760827357193E-4	0.0017089605930776842	0.0	0.046474233906195914	5.696535310258947E-5
48-49	1.5190760827357193E-4	0.0017089605930776842	0.0	0.05435444108538746	5.696535310258947E-5
50-51	1.9937873585906316E-4	0.0018893508779025507	0.0	0.06137067374252306	5.696535310258947E-5
52-53	2.088729613761614E-4	0.0019178335544538455	0.0	0.06912745598999233	6.645957861968772E-5
54-55	2.088729613761614E-4	0.0019273277799709437	0.0	0.07691272091401288	8.54480296538842E-5
56-57	2.278614124103579E-4	0.001936822005488042	0.0	0.08423276878769564	9.494225517098246E-5
58-59	2.278614124103579E-4	0.0019463162310051404	0.0	0.09111608228759185	9.494225517098246E-5
60-61	2.7533253999584913E-4	0.0019842931330735334	0.0	0.09749620183508188	9.494225517098246E-5
62-63	3.417921186155368E-4	0.0020697411627274174	0.0	0.10418013659911905	1.1393070620517895E-4
64-65	3.417921186155368E-4	0.002316591026171972	0.0	0.11118687503073756	1.1393070620517895E-4
66-67	3.417921186155368E-4	0.0023355794772061686	0.0	0.11768092528443276	1.1393070620517895E-4
68-69	3.417921186155368E-4	0.0024210275068600527	0.0	0.12391863144916632	1.1393070620517895E-4
70-71	3.5128634413263506E-4	0.0024684986344455442	0.0	0.13014684338838275	1.1393070620517895E-4
72-73	3.892632462010281E-4	0.0024969813109968386	0.0	0.13515030023589353	1.5190760827357193E-4
74-75	3.9875747171812633E-4	0.002553946664099428	0.0	0.13929927678686543	1.5190760827357193E-4
76-77	3.9875747171812633E-4	0.0027058542723729997	0.0	0.14313494389577314	1.5190760827357193E-4
78-79	3.9875747171812633E-4	0.0027533253999584913	0.0	0.14684718607295857	1.5190760827357193E-4
80-81	3.9875747171812633E-4	0.0027628196254755894	0.0	0.14837575638121137	1.5190760827357193E-4
82-83	3.9875747171812633E-4	0.0027723138509926875	0.0	0.14906883484395955	1.5190760827357193E-4
84-85	3.9875747171812633E-4	0.0027723138509926875	0.0	0.14961949992395124	1.5190760827357193E-4
86-87	3.9875747171812633E-4	0.0028102907530610805	0.0	0.1498283728853274	1.5190760827357193E-4
88-89	3.9875747171812633E-4	0.002971692586851751	0.0	0.1498948324639471	1.5190760827357193E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6245	0.0	43.538105	1
GTATCAA	10395	0.0	34.416275	1
TCAACGC	12495	0.0	27.75543	4
ATCAACG	12530	0.0	27.678429	3
CAACGCA	12710	0.0	27.285929	5
AACGCAG	13065	0.0	26.769764	6
TATCAAC	13280	0.0	26.654305	2
ACGCAGA	15060	0.0	23.083838	7
CGCAGAG	15275	0.0	22.786478	8
GTACATG	14640	0.0	20.153309	1
TACATGG	14475	0.0	19.685186	2
GCAGAGT	18305	0.0	19.037659	9
ACATGGG	14680	0.0	18.922703	3
AGAGTAC	15775	0.0	18.075605	10-11
GAGTACT	9990	0.0	18.073517	12-13
CATGGGG	10910	0.0	17.745577	4
CAGAGTA	17610	0.0	17.279528	10-11
GTACTTT	11585	0.0	15.5129	14-15
AGTACTT	11160	0.0	15.349036	12-13
GTGGTAT	3420	0.0	14.521962	1
>>END_MODULE