FastQCFastQC Report
Fri 27 May 2016
SRR2138802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10021773
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106450.042.2307551
GTATCAA177450.034.929071
TCAACGC205550.029.1870884
ATCAACG207800.028.91233
CAACGCA209950.028.65595
AACGCAG218350.027.6701456
TATCAAC222950.027.2132192
ACGCAGA251350.023.9533777
CGCAGAG253300.023.7522938
CTTATAC86550.022.455551
GTACATG247300.021.4242841
TACATGG246750.020.7700042
ACATGGG250150.019.8963493
GCAGAGT313950.019.0563649
TATACAC130600.018.8928853
GAGTACT178600.018.18006112-13
TATACCG8150.018.1438885
AGAGTAC273650.017.77121410-11
CAGAGTA304300.016.93234410-11
CATGGGG180100.016.5384714
Produced by FastQC (version 0.11.5)