##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138802_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10021773
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44277344936869	33.0	31.0	34.0	31.0	34.0
2	32.63079626728724	34.0	31.0	34.0	31.0	34.0
3	32.71249209097033	34.0	31.0	34.0	31.0	34.0
4	36.17422625717026	37.0	35.0	37.0	35.0	37.0
5	35.99382045472393	37.0	35.0	37.0	35.0	37.0
6	36.05720893897716	37.0	35.0	37.0	35.0	37.0
7	36.058723042319954	37.0	35.0	37.0	35.0	37.0
8	36.042275653220244	37.0	35.0	37.0	35.0	37.0
9	37.90403284927727	39.0	38.0	39.0	35.0	39.0
10-11	37.780187547652496	39.0	37.5	39.0	35.0	39.0
12-13	37.786920587804175	39.0	38.0	39.0	35.0	39.0
14-15	39.11022211339251	40.0	38.5	41.0	36.0	41.0
16-17	39.08144337334322	40.0	38.0	41.0	36.0	41.0
18-19	39.14967960260125	40.0	38.5	41.0	36.0	41.0
20-21	39.168946623871975	40.0	39.0	41.0	36.0	41.0
22-23	39.12839500570204	40.0	39.0	41.0	36.0	41.0
24-25	39.10023253205868	40.0	39.0	41.0	36.0	41.0
26-27	39.01124080736898	40.0	39.0	41.0	36.0	41.0
28-29	38.94755552412235	40.0	38.0	41.0	35.0	41.0
30-31	38.81328922458826	40.0	38.0	41.0	35.0	41.0
32-33	38.68462711981946	40.0	38.0	41.0	35.0	41.0
34-35	38.64117914004683	40.0	38.0	41.0	35.0	41.0
36-37	38.588894957356274	40.0	38.0	41.0	35.0	41.0
38-39	38.51608080384882	40.0	38.0	41.0	34.5	41.0
40-41	38.44268986102537	40.0	38.0	41.0	34.0	41.0
42-43	38.461984518014056	40.0	38.0	41.0	34.0	41.0
44-45	38.40320274377962	40.0	38.0	41.0	34.0	41.0
46-47	38.356756598816304	40.0	38.0	41.0	34.0	41.0
48-49	38.315687241253606	40.0	38.0	41.0	34.0	41.0
50-51	38.24309729181587	40.0	38.0	41.0	34.0	41.0
52-53	37.9984672384246	40.0	37.0	41.0	33.0	41.0
54-55	37.85445690175962	40.0	37.0	41.0	33.0	41.0
56-57	37.75910270305886	39.0	37.0	41.0	33.0	41.0
58-59	37.632011596475294	39.0	36.0	41.0	33.0	41.0
60-61	37.45275896738365	39.0	36.0	41.0	32.5	41.0
62-63	37.20708976365151	39.0	35.5	40.0	32.0	41.0
64-65	36.97435361925969	38.5	35.0	40.0	32.0	41.0
66-67	36.7139953557398	38.0	35.0	40.0	31.5	41.0
68-69	36.47933381179372	37.5	35.0	40.0	31.0	41.0
70-71	36.17248288833375	37.0	35.0	39.5	31.0	41.0
72-73	35.88239432484552	36.5	34.5	39.0	31.0	41.0
74-75	35.43344935723958	36.0	34.0	39.0	30.5	40.0
76-77	33.500909178121475	34.5	32.0	36.5	27.5	39.0
78-79	34.690191615529336	35.0	34.0	37.0	30.0	39.0
80-81	34.663479192544685	35.0	34.0	37.0	31.0	39.0
82-83	34.394241481505006	35.0	34.0	36.0	30.0	38.5
84-85	34.11368994513649	35.0	34.0	36.0	30.0	37.0
86-87	33.88215138723231	35.0	34.0	36.0	30.0	37.0
88-89	33.74667904566587	35.0	34.0	35.0	30.0	36.5
90-91	33.57867716271598	35.0	33.5	35.0	30.0	36.0
92-93	33.539562300079155	35.0	33.5	35.0	30.0	36.0
94-95	33.41131035660349	35.0	33.0	35.0	30.0	36.0
96-97	33.430900562814976	35.0	33.0	35.0	30.0	35.5
98-99	33.52981995211418	35.0	33.0	35.0	30.0	35.0
100-101	32.25924593430164	34.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	5.0
20	45.0
21	164.0
22	789.0
23	2644.0
24	7125.0
25	15366.0
26	28521.0
27	47560.0
28	70587.0
29	99772.0
30	133847.0
31	176045.0
32	232893.0
33	319086.0
34	471412.0
35	735215.0
36	1327884.0
37	2436596.0
38	2799366.0
39	1099199.0
40	17650.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.423578442656805	20.339225404526722	13.955953702004626	24.281242450811845
2	14.466000981673988	21.074029973286855	42.03390716724789	22.426061877791266
3	18.206982105184803	26.103725147836236	30.39501227453365	25.294280472445312
4	12.395371223423156	18.15687513776299	38.946326790923855	30.501426847890002
5	13.084687814098494	37.895396776027944	35.91864364431036	13.101271765563197
6	29.83325917774003	38.29001026869537	18.870082294248665	13.00664825931594
7	26.4568754450934	32.408447088154965	23.340859945640357	17.793817521111286
8	23.94578284700721	37.55629867090384	21.350603331366617	17.14731515072233
9	25.248705992442655	16.982553885425265	21.311967453264007	36.456772668868076
10-11	23.956514481020474	26.915881052185075	29.25322195982687	19.87438250696758
12-13	24.78648661499169	24.283357323825545	29.29209413373219	21.63806192745058
14-15	21.875108202710937	25.81180097306854	26.634236370499654	25.678854453720874
16-17	21.680409756206593	29.013050995812712	27.30139125207898	22.00514799590172
18-19	21.71712530307761	28.272128095497674	28.799714381876342	21.21103221954838
20-21	22.373149711734168	27.50209325389995	28.658664055475246	21.466092978890643
22-23	22.067516889850502	27.571284454930776	28.514036446490614	21.847162208728104
24-25	21.919018143802145	27.637243333441795	28.66305117470883	21.780687348047227
26-27	21.936268397945522	27.79476064392878	28.51849483297691	21.750476125148786
28-29	21.794066767473772	27.79466221876541	28.61245861938224	21.79881239437858
30-31	22.103039618267072	27.77373964421328	28.424740909309865	21.698479828209777
32-33	22.08963645186358	27.741178041000364	28.4337306744439	21.73545483269216
34-35	21.995883529581135	27.741422116888725	28.4917639907731	21.770930362757042
36-37	21.906874317759367	27.86943260821718	28.429069509166705	21.794623564856742
38-39	21.794727116026017	27.77899521429632	28.600950488769545	21.825327180908122
40-41	22.063995303548158	27.636570032866963	28.468704664486467	21.830729999098413
42-43	22.00256751734886	27.700692663919785	28.347827002665337	21.948912816066017
44-45	22.007106899429967	27.87265310391171	28.18752397794701	21.932716018711314
46-47	22.15649460224353	27.762011112565254	27.90241924335478	22.179075041836434
48-49	22.088440792210935	27.819360623589773	27.91786631494998	22.174332269249312
50-51	21.98319707300459	27.94162990399117	27.928732979400507	22.146440043603736
52-53	22.17456173372758	27.87684736293945	27.86783012343978	22.08076077989319
54-55	22.122929126053016	27.78407127589764	27.8351486337449	22.257850964304442
56-57	22.199969512179972	27.99560913554663	27.64936091798263	22.155060434290768
58-59	22.09807725309246	28.079890813522717	27.82482742676475	21.997204506620076
60-61	22.241125713826563	28.11833415329703	27.61698438497789	22.02355574789852
62-63	22.150916470712943	28.21224666118794	27.558053783455083	22.07878308464403
64-65	22.16034417979527	28.115396974816132	27.56708261452092	22.157176230867677
66-67	22.242328352400918	28.079666179855334	27.46333040702826	22.214675060715493
68-69	22.165680710548592	28.03183864114891	27.586955906659156	22.215524741643343
70-71	22.20564203366953	27.804588675987585	27.627196755744194	22.362572534598698
72-73	22.23118817002942	27.86027936939429	27.597928930366983	22.31060353020931
74-75	22.1760754355586	27.96098619020375	27.527773061768414	22.335165312469236
76-77	22.221368093982765	27.94766915869368	27.516424125754195	22.31453862156935
78-79	22.207674489498977	27.945692963179653	27.49765909881805	22.348973448503322
80-81	22.21242894721263	27.961091896936964	27.522237466490907	22.304241689359504
82-83	22.252880605736213	27.934986930620585	27.61304514751043	22.199087316132772
84-85	22.367085486359446	27.873864559294926	27.51020787294086	22.248842081404764
86-87	22.192502021733336	27.921624072078753	27.589233297417604	22.296640608770304
88-89	22.218836723904	27.899219259262402	27.560186522083473	22.32175749475012
90-91	22.289607638746805	27.932008196986114	27.53323699110737	22.245147173159715
92-93	22.265218662796578	27.89992292618132	27.58640057067423	22.24845784034787
94-95	22.14721019876392	27.94770854998135	27.62012633140842	22.28495491984631
96-97	22.173737121167182	27.944527261051057	27.657904549200897	22.223831068580868
98-99	22.409634443012642	28.54859760768261	27.69445915680602	21.34730879249873
100-101	23.21180548569558	31.124964622910404	24.742225088157696	20.921004803236315
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2035.0
1	1782.0
2	2285.0
3	3259.0
4	4465.5
5	7278.0
6	9686.5
7	11380.5
8	12451.0
9	12070.5
10	10890.5
11	9924.0
12	10812.5
13	14204.5
14	21781.5
15	31637.5
16	39477.0
17	44127.0
18	45493.0
19	44002.5
20	41776.5
21	40647.5
22	41950.5
23	47942.5
24	58862.0
25	73604.0
26	92607.5
27	114177.5
28	137606.5
29	162139.0
30	183774.0
31	203686.5
32	225435.5
33	247097.5
34	267642.5
35	288653.5
36	310404.5
37	329873.5
38	345857.5
39	359011.0
40	371923.0
41	385422.5
42	394838.5
43	403593.5
44	413781.0
45	423292.0
46	433112.0
47	439672.0
48	437564.5
49	429089.5
50	420298.5
51	408215.5
52	388683.0
53	365968.0
54	341091.5
55	312645.5
56	284355.0
57	255970.5
58	226648.0
59	196214.5
60	163983.5
61	133380.5
62	105648.5
63	81502.5
64	61239.5
65	45651.5
66	34454.5
67	26273.0
68	19841.5
69	14530.0
70	10645.5
71	7601.5
72	5228.5
73	3618.0
74	2527.5
75	1684.0
76	1120.0
77	725.0
78	496.5
79	371.0
80	281.5
81	200.0
82	136.5
83	95.0
84	68.5
85	52.5
86	39.0
87	27.0
88	16.0
89	11.0
90	9.5
91	8.5
92	7.5
93	5.5
94	3.0
95	2.0
96	2.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0011574798191896784
3	0.0021054158780088113
4	3.193047777074975E-4
5	3.5921787492093464E-4
6	5.188702637746835E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	9.429469216674535E-4
14-15	7.383922984485878E-4
16-17	8.980446873023366E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	6.989171327403886E-5
28-29	2.1979697353554522E-4
30-31	5.002158681579035E-5
32-33	0.0011683564544222643
34-35	0.001961718521761595
36-37	0.0015056815224885663
38-39	7.577488978821986E-4
40-41	4.610389081492161E-5
42-43	3.097249451490027E-5
44-45	6.927059160783137E-4
46-47	2.631138854774712E-5
48-49	0.0
50-51	0.0
52-53	0.0010939714429044661
54-55	0.003814942287247009
56-57	0.006630467955351389
58-59	0.004624724266854289
60-61	2.8757383317199126E-4
62-63	2.2796356048078427E-5
64-65	1.3247768874125116E-4
66-67	1.4563540874470724E-4
68-69	0.0
70-71	9.539114123934317E-5
72-73	3.0145511782463715E-5
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	3.274591312993886E-5
88-89	3.11860044112935E-4
90-91	1.6825760966913218E-4
92-93	6.149468993352423E-4
94-95	3.959563272674908E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	184.0
20-21	624.0
22-23	1473.0
24-25	3024.0
26-27	5125.0
28-29	10213.0
30-31	23223.0
32-33	28985.0
34-35	39366.0
36-37	59717.0
38-39	71637.0
40-41	72813.0
42-43	82192.0
44-45	96885.0
46-47	96964.0
48-49	91496.0
50-51	85119.0
52-53	82419.0
54-55	90558.0
56-57	93462.0
58-59	94946.0
60-61	94767.0
62-63	92126.0
64-65	95678.0
66-67	102598.0
68-69	95871.0
70-71	94392.0
72-73	91447.0
74-75	91676.0
76-77	89981.0
78-79	96365.0
80-81	97977.0
82-83	95163.0
84-85	94728.0
86-87	97468.0
88-89	104829.0
90-91	110396.0
92-93	115903.0
94-95	136315.0
96-97	201849.0
98-99	836071.0
100-101	6055748.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.46880299689937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.78833065854822	76.74101144475918
2	7.143513077559878	12.210950238634792
3	1.7616837861631844	4.51707013387239
4	0.6207155848900135	2.1220727216827893
5	0.2918753400547972	1.2473117969398237
6	0.1364738639131774	0.6998554673412609
7	0.07661890266504057	0.45839681284018713
8	0.04806133731538215	0.328619597662074
9	0.032461022226016434	0.24969642423420335
>10	0.09840476287871058	1.2477729617304312
>50	0.0014575116679521791	0.08448702981080172
>100	3.495009740402203E-4	0.05255394756390228
>500	3.64342147055065E-5	0.02279439649238551
>1k	1.821692891612135E-5	0.017407026435718738
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.9956548606718593E-5	0.0	9.978274303359296E-6	0.0
3	0.0	1.9956548606718593E-5	0.0	9.978274303359296E-6	0.0
4	0.0	7.982619442687437E-5	0.0	1.9956548606718593E-5	0.0
5	0.0	7.982619442687437E-5	0.0	1.9956548606718593E-5	0.0
6	0.0	7.982619442687437E-5	0.0	1.9956548606718593E-5	0.0
7	0.0	7.982619442687437E-5	0.0	1.9956548606718593E-5	9.978274303359296E-6
8	0.0	9.978274303359296E-5	0.0	1.9956548606718593E-5	9.978274303359296E-6
9	0.0	1.9956548606718592E-4	0.0	1.0976101733695225E-4	9.978274303359296E-6
10-11	0.0	1.9956548606718592E-4	0.0	1.2971756594367084E-4	1.4967411455038946E-5
12-13	0.0	1.9956548606718592E-4	0.0	1.3969584024703014E-4	1.9956548606718593E-5
14-15	0.0	2.8438081764573994E-4	0.0	1.6963066315710804E-4	5.488050866847613E-5
16-17	0.0	2.943590919490992E-4	0.0	1.9956548606718592E-4	5.9869645820155775E-5
18-19	0.0	3.1930477770749743E-4	0.0	2.3448944612894346E-4	6.984792012351507E-5
20-21	0.0	3.492396006175753E-4	0.0	2.594351318873417E-4	6.984792012351507E-5
22-23	0.0	3.891526978310125E-4	0.0	2.8936995479741955E-4	7.982619442687436E-5
24-25	0.0	4.140983835894108E-4	0.0	3.442504634658957E-4	9.978274303359296E-5
26-27	0.0	4.290657950444497E-4	0.0	4.6897889225788693E-4	9.978274303359296E-5
28-29	0.0	4.4403320649948866E-4	0.0	0.001347067030953505	9.978274303359296E-5
30-31	0.0	5.288485380780427E-4	0.0	0.004764625979854064	9.978274303359296E-5
32-33	0.0	5.38826812381402E-4	0.0	0.012507766839260878	1.0976101733695225E-4
34-35	4.989137151679648E-6	5.887181838981985E-4	0.0	0.019891689823746755	1.0976101733695225E-4
36-37	9.978274303359296E-6	6.38609555414995E-4	0.0	0.030708139168588233	1.0976101733695225E-4
38-39	9.978274303359296E-6	6.38609555414995E-4	0.0	0.04729702019792306	1.1973929164031154E-4
40-41	2.494568575839824E-5	6.785226526284321E-4	0.0	0.061995018246771304	1.2971756594367084E-4
42-43	2.9934822910077888E-5	6.984792012351506E-4	0.0	0.07323055511235388	1.2971756594367084E-4
44-45	2.9934822910077888E-5	7.93272807117064E-4	0.0	0.08567346316864291	1.347067030953505E-4
46-47	2.9934822910077888E-5	8.681098643922588E-4	0.0	0.09798665365898829	1.5965238885374872E-4
48-49	2.9934822910077888E-5	8.681098643922588E-4	0.0	0.10833911324872356	1.8958721176382662E-4
50-51	2.9934822910077888E-5	9.479360588191331E-4	0.0	0.11791326744279679	1.8958721176382662E-4
52-53	2.9934822910077888E-5	9.479360588191331E-4	0.0	0.12660933349817444	2.2451117182558414E-4
54-55	2.9934822910077888E-5	9.529251959708128E-4	0.0	0.13537524747367558	2.69413406190701E-4
56-57	2.9934822910077888E-5	9.579143331224924E-4	0.0	0.14379691098571082	2.793916804940603E-4
58-59	2.9934822910077888E-5	9.579143331224924E-4	0.0	0.15180447611415665	2.793916804940603E-4
60-61	2.9934822910077888E-5	9.77870881729211E-4	0.0	0.15997169363145625	2.8438081764573994E-4
62-63	2.9934822910077888E-5	9.978274303359296E-4	0.0	0.16744043194752067	2.8936995479741955E-4
64-65	2.9934822910077888E-5	0.0011275449962796003	0.0	0.17491914853788845	2.943590919490992E-4
66-67	2.9934822910077888E-5	0.0011674580934930377	0.0	0.18200871243042524	3.043373662524585E-4
68-69	2.9934822910077888E-5	0.0012023820535547953	0.0	0.1897418750155287	3.0932650340413816E-4
70-71	2.9934822910077888E-5	0.0012223386021615137	0.0	0.19599326386658328	3.0932650340413816E-4
72-73	2.9934822910077888E-5	0.0012273277393131934	0.0	0.20051342212600504	3.6919614922429397E-4
74-75	2.9934822910077888E-5	0.0012472842879199119	0.0	0.2043201337727366	3.6919614922429397E-4
76-77	2.9934822910077888E-5	0.0013071539337400678	0.0	0.20813682369377157	3.6919614922429397E-4
78-79	2.9934822910077888E-5	0.0013370887566501457	0.0	0.2114346433510318	3.6919614922429397E-4
80-81	2.9934822910077888E-5	0.0013370887566501457	0.0	0.21306609119963105	3.6919614922429397E-4
82-83	2.9934822910077888E-5	0.0013370887566501457	0.0	0.21367975506928763	3.6919614922429397E-4
84-85	2.9934822910077888E-5	0.0013620344424085439	0.0	0.21414873396154555	3.6919614922429397E-4
86-87	2.9934822910077888E-5	0.0014069366767736607	0.0	0.21438821254482615	3.6919614922429397E-4
88-89	2.9934822910077888E-5	0.001446849773987098	0.0	0.2144231365048879	3.7917442352765324E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10645	0.0	42.230755	1
GTATCAA	17745	0.0	34.92907	1
TCAACGC	20555	0.0	29.187088	4
ATCAACG	20780	0.0	28.9123	3
CAACGCA	20995	0.0	28.6559	5
AACGCAG	21835	0.0	27.670145	6
TATCAAC	22295	0.0	27.213219	2
ACGCAGA	25135	0.0	23.953377	7
CGCAGAG	25330	0.0	23.752293	8
CTTATAC	8655	0.0	22.45555	1
GTACATG	24730	0.0	21.424284	1
TACATGG	24675	0.0	20.770004	2
ACATGGG	25015	0.0	19.896349	3
GCAGAGT	31395	0.0	19.056364	9
TATACAC	13060	0.0	18.892885	3
GAGTACT	17860	0.0	18.180061	12-13
TATACCG	815	0.0	18.143888	5
AGAGTAC	27365	0.0	17.771214	10-11
CAGAGTA	30430	0.0	16.932344	10-11
CATGGGG	18010	0.0	16.538471	4
>>END_MODULE