FastQCFastQC Report
Fri 27 May 2016
SRR2138801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10799191
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121350.045.6672361
GTATCAA213600.035.9777871
TCAACGC254950.029.4951524
ATCAACG256350.029.2852823
CAACGCA260050.029.0140975
TATCAAC268400.028.2856122
AACGCAG270100.028.0757646
CTTATAC140950.025.8214051
ACGCAGA312500.024.1176557
CGCAGAG317350.023.7890288
TATACAC181100.022.5241623
GTACATG317400.021.601591
TACATGG313950.021.206152
ACATGGG317750.020.407023
TTATACA188400.020.3950482
GCAGAGT383500.019.5864549
GAGTACT205400.018.18179912-13
AGAGTAC336450.017.81514710-11
CATGGGG232700.017.6199674
CAGAGTA368050.017.51446210-11
Produced by FastQC (version 0.11.5)