##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138801_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10799191
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41232820125137	33.0	31.0	34.0	31.0	34.0
2	32.60397727940917	34.0	31.0	34.0	31.0	34.0
3	32.681866076820015	34.0	31.0	34.0	31.0	34.0
4	36.1546271382736	37.0	35.0	37.0	35.0	37.0
5	35.96808455374111	37.0	35.0	37.0	35.0	37.0
6	36.03405468057746	37.0	35.0	37.0	35.0	37.0
7	36.032272047045005	37.0	35.0	37.0	35.0	37.0
8	36.016222141084455	37.0	35.0	37.0	35.0	37.0
9	37.87422715275616	39.0	38.0	39.0	35.0	39.0
10-11	37.745580432830565	39.0	37.5	39.0	35.0	39.0
12-13	37.75095666888381	39.0	37.5	39.0	35.0	39.0
14-15	39.056663179677074	40.0	38.5	41.0	36.0	41.0
16-17	39.02423528762479	40.0	38.0	41.0	36.0	41.0
18-19	39.09619086281556	40.0	38.5	41.0	36.0	41.0
20-21	39.115394675778724	40.0	39.0	41.0	36.0	41.0
22-23	39.07407968194755	40.0	39.0	41.0	36.0	41.0
24-25	39.04050808187721	40.0	39.0	41.0	36.0	41.0
26-27	38.94596562752902	40.0	38.0	41.0	35.5	41.0
28-29	38.88302546659505	40.0	38.0	41.0	35.0	41.0
30-31	38.755240003191815	40.0	38.0	41.0	35.0	41.0
32-33	38.615093777790605	40.0	38.0	41.0	35.0	41.0
34-35	38.56555076245856	40.0	38.0	41.0	35.0	41.0
36-37	38.51770830930183	40.0	38.0	41.0	34.5	41.0
38-39	38.436404720192016	40.0	38.0	41.0	34.0	41.0
40-41	38.36028630506863	40.0	38.0	41.0	34.0	41.0
42-43	38.38032290610758	40.0	38.0	41.0	34.0	41.0
44-45	38.31610686984176	40.0	38.0	41.0	34.0	41.0
46-47	38.26252435356328	40.0	38.0	41.0	34.0	41.0
48-49	38.21720846992835	40.0	38.0	41.0	34.0	41.0
50-51	38.13818057866817	40.0	37.5	41.0	33.0	41.0
52-53	37.88481649545013	40.0	37.0	41.0	33.0	41.0
54-55	37.72783715995929	39.5	37.0	41.0	33.0	41.0
56-57	37.6169376125394	39.0	36.0	41.0	33.0	41.0
58-59	37.476562377879006	39.0	36.0	41.0	32.5	41.0
60-61	37.27328682234435	39.0	35.5	40.0	32.0	41.0
62-63	37.01815746074297	39.0	35.0	40.0	32.0	41.0
64-65	36.78750809818053	38.0	35.0	40.0	31.0	41.0
66-67	36.526271245006114	38.0	35.0	40.0	31.0	41.0
68-69	36.29166119765161	37.0	35.0	40.0	31.0	41.0
70-71	35.98619438573012	37.0	35.0	39.0	31.0	41.0
72-73	35.69936147208931	36.0	34.0	39.0	31.0	40.5
74-75	35.255847566031036	36.0	34.0	39.0	30.5	40.0
76-77	33.348227944901794	34.5	32.0	36.5	27.5	39.0
78-79	34.53792280398929	35.0	34.0	37.0	30.0	39.0
80-81	34.52160093159566	35.0	34.0	37.0	30.0	39.0
82-83	34.263406826642566	35.0	34.0	36.0	30.0	38.5
84-85	33.99163033807645	35.0	34.0	36.0	30.0	37.0
86-87	33.76880659779258	35.0	34.0	35.5	30.0	37.0
88-89	33.63980859609629	35.0	33.5	35.0	30.0	36.5
90-91	33.48248950062464	35.0	33.0	35.0	29.5	36.0
92-93	33.45756968100963	35.0	33.0	35.0	30.0	36.0
94-95	33.33417185859774	35.0	33.0	35.0	29.5	36.0
96-97	33.360937598096	35.0	33.0	35.0	30.0	35.0
98-99	33.470712335641316	35.0	33.0	35.0	30.0	35.0
100-101	32.20376996508686	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	9.0
20	32.0
21	183.0
22	886.0
23	3163.0
24	8348.0
25	18198.0
26	33712.0
27	55772.0
28	82519.0
29	113859.0
30	151680.0
31	199816.0
32	263348.0
33	360226.0
34	532189.0
35	829339.0
36	1490868.0
37	2643800.0
38	2884627.0
39	1109570.0
40	17046.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.479162652091254	20.38848095195279	14.040977699162834	24.09137869679312
2	14.615419589870656	20.941143047264855	41.954014420301135	22.48942294256335
3	18.2192342774066	26.28076803763329	30.43712583167808	25.062871853282033
4	12.506993611159109	18.124949185777908	38.528940539905854	30.839116663157128
5	13.1720325310457	37.92545550529342	35.672021187571886	13.230490776088994
6	29.936258783689617	38.028999544870956	18.706692711962578	13.328048959476845
7	26.513856454617756	32.50200871528247	23.05592150374968	17.928213326350097
8	24.308043074708095	37.337343139870384	21.15528839151007	17.19932539391145
9	25.430877183300122	16.823278706710532	20.98524787643815	36.7605962335512
10-11	24.178973221234813	26.77019047074915	28.9388019898898	20.112034318126238
12-13	25.078987019686714	24.12282263371492	29.014616933500243	21.78357341309812
14-15	22.226189106481055	25.608545690381007	26.30835222567749	25.85691297746045
16-17	21.952695572844213	28.927197663489157	26.926832728356814	22.193274035309813
18-19	21.952459216620948	28.00893603974594	28.487481145578403	21.551123598054705
20-21	22.665592170282828	27.106724021499495	28.376270360082074	21.851413448135602
22-23	22.3620918562774	27.261122335503384	28.270984242138663	22.105801566080554
24-25	22.12883990342718	27.43266598638851	28.470845115928885	21.967648994255427
26-27	22.08164716702052	27.477527627877024	28.428443190413148	22.012382014689308
28-29	21.79917999555965	27.34715133086955	28.461295357414112	22.392373316156686
30-31	22.15475253913058	27.208939273432193	28.44333644499761	22.192971742439617
32-33	22.011311317450744	27.49295764947666	28.41171832980491	22.084012703267685
34-35	22.03401325362884	27.688925075959865	28.532147723407142	21.744913947004154
36-37	22.1322771882152	27.45426020549396	28.208688417454464	22.20477418883638
38-39	22.088943793863063	27.425912013437088	28.419093370397334	22.06605082230252
40-41	22.252975738795723	27.287766416409394	28.388855678004365	22.070402166790526
42-43	22.298309148400854	27.32475524944039	28.297995695405042	22.078939906753718
44-45	22.156865196697602	27.576998573046946	28.089444597663103	22.176691632592345
46-47	22.47022294366468	27.35937723703403	27.696597621190435	22.473802198110857
48-49	22.335935116667518	27.460324355589993	27.78942586316253	22.41431466457996
50-51	22.170051944584156	27.66981811563954	27.791951162692985	22.368178777083322
52-53	22.353124661075046	27.639847270696606	27.8141895142426	22.192838553985748
54-55	22.27007615751576	27.563532778121314	27.639289046747617	22.527102017615306
56-57	22.49993689438409	27.69990096250627	27.45358977956774	22.3465723635419
58-59	22.207749671502693	27.917601958053005	27.730871468315204	22.143776902129094
60-61	22.47976353447666	28.03039284091528	27.352111473741488	22.137732150866572
62-63	22.287234825703194	28.180982043001407	27.306623301242517	22.225159830052878
64-65	22.298087560401783	28.032385098157487	27.35170019096372	22.317827150477008
66-67	22.400522492673048	27.931587479995727	27.222707962982646	22.44518206434858
68-69	22.340542798828718	27.8302603894537	27.361962331010968	22.467234480706612
70-71	22.35554092093114	27.611926795916762	27.41277134604971	22.61976093710239
72-73	22.388872981890064	27.676799577242516	27.342250864643464	22.592076576223956
74-75	22.312948228514134	27.760675827539483	27.346259790472104	22.58011615347428
76-77	22.44591052431056	27.668516422644885	27.26111405075739	22.624459002287168
78-79	22.38721635212566	27.706711813117575	27.233296506358162	22.672775328398608
80-81	22.41844047291299	27.772899791896457	27.192742806106075	22.61591692908448
82-83	22.473842157585597	27.651296588224017	27.32012665106995	22.554734603120437
84-85	22.5851345993994	27.67484043066095	27.21452804779943	22.52549692214022
86-87	22.37409156441499	27.75998136433365	27.294363045012	22.571564026239358
88-89	22.436920423483436	27.688822070217128	27.286471939255623	22.58778556704381
90-91	22.477166029872503	27.65134050795653	27.337370882410728	22.53412257976024
92-93	22.431715714680845	27.636899331485893	27.343433473200633	22.58795148063263
94-95	22.300399962712607	27.697698277185857	27.421781896352655	22.580119863748884
96-97	22.273799643258606	27.7232425367028	27.460723670318416	22.542234149720176
98-99	22.545322561751277	28.331112300145616	27.494483101790706	21.6290820363124
100-101	23.38960414022842	30.917430455565064	24.488577156465823	21.204388247740695
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2489.0
1	2214.5
2	2874.0
3	4098.5
4	5700.0
5	9044.0
6	11655.5
7	13556.5
8	14768.0
9	14414.0
10	13015.0
11	11560.0
12	12404.5
13	16265.0
14	25171.0
15	36947.0
16	46128.0
17	51218.5
18	52096.0
19	49847.5
20	46532.0
21	43725.5
22	43940.5
23	49342.0
24	59872.5
25	75744.5
26	96019.5
27	117652.5
28	140586.5
29	164730.5
30	185595.0
31	204887.0
32	226636.5
33	249597.0
34	271934.0
35	292982.0
36	316140.5
37	337981.5
38	353908.5
39	366038.5
40	380507.5
41	396323.0
42	407422.0
43	417990.5
44	431239.0
45	443262.0
46	459375.5
47	475840.5
48	478339.0
49	472916.5
50	467765.0
51	455290.0
52	433214.0
53	410037.5
54	386529.0
55	359407.0
56	330397.0
57	298341.5
58	264721.5
59	230815.0
60	193252.0
61	156621.0
62	124346.5
63	96523.5
64	72213.5
65	52694.5
66	39083.5
67	29659.0
68	22257.0
69	15768.0
70	10942.5
71	7556.0
72	5112.5
73	3478.5
74	2378.5
75	1621.0
76	1140.0
77	776.0
78	506.0
79	335.5
80	241.5
81	180.0
82	140.5
83	104.5
84	73.0
85	52.5
86	37.5
87	27.5
88	19.5
89	13.5
90	10.5
91	10.5
92	10.0
93	6.0
94	2.5
95	2.0
96	2.0
97	2.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0010185948188155946
3	0.0022316486484959845
4	4.537376920178558E-4
5	2.7779858694970764E-4
6	5.185573623061209E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	9.86184983671462E-4
14-15	5.880070090435478E-4
16-17	1.0648945833072127E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.633118410692125E-5
28-29	1.8080323925229053E-4
30-31	3.713755774600092E-5
32-33	0.0010982102430441643
34-35	0.0020630187827125
36-37	0.0014203866264270665
38-39	8.632552298053821E-4
40-41	6.175083178370413E-5
42-43	5.742647007097481E-5
44-45	7.530802793715429E-4
46-47	2.438183633165715E-5
48-49	0.0
50-51	0.0
52-53	0.001048430306698973
54-55	0.0038453479966571785
56-57	0.006642255186656057
58-59	0.00464559168191324
60-61	2.3480360217945748E-4
62-63	6.332563768521364E-5
64-65	1.5479914170815195E-4
66-67	2.48558401795413E-4
68-69	5.470515779921588E-6
70-71	8.299750697621713E-5
72-73	1.1191164620296934E-5
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	1.2148412047653116E-5
88-89	3.1387827566599585E-4
90-91	1.0613398229060856E-4
92-93	4.564383771105282E-4
94-95	2.320786602253664E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	193.0
20-21	751.0
22-23	1681.0
24-25	3619.0
26-27	5467.0
28-29	10899.0
30-31	25027.0
32-33	30076.0
34-35	41029.0
36-37	61962.0
38-39	73798.0
40-41	76355.0
42-43	86097.0
44-45	102475.0
46-47	103026.0
48-49	98393.0
50-51	90516.0
52-53	82936.0
54-55	93968.0
56-57	99303.0
58-59	101378.0
60-61	109200.0
62-63	106657.0
64-65	110318.0
66-67	118442.0
68-69	103343.0
70-71	102034.0
72-73	97243.0
74-75	98069.0
76-77	95785.0
78-79	103176.0
80-81	106423.0
82-83	101764.0
84-85	100680.0
86-87	105133.0
88-89	113517.0
90-91	120693.0
92-93	126576.0
94-95	149560.0
96-97	221970.0
98-99	909170.0
100-101	6510489.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.22119937513777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1763482861998	75.1053900855876
2	7.3865666798584915	12.442110100842232
3	1.9126661060811012	4.832611003749745
4	0.7145207220575356	2.4071116876030048
5	0.33448440517426786	1.408533888802819
6	0.1705766763354646	0.8619703359838505
7	0.08963601126781788	0.528447666332529
8	0.05507490275293016	0.3710779492256684
9	0.041526852967503386	0.31476972268783215
>10	0.11543680550939997	1.4600752289716479
>50	0.0024614742344371047	0.14309056290620992
>100	6.462813843655672E-4	0.08610750962699147
>500	3.653118501864946E-5	0.021567601037404408
>1k	1.826499183677001E-5	0.017136656642438363
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.259952898323587E-6	0.0	1.8519905796647174E-5	0.0
3	0.0	1.8519905796647174E-5	0.0	1.8519905796647174E-5	0.0
4	0.0	7.40796231865887E-5	0.0	2.7779858694970763E-5	9.259952898323587E-6
5	9.259952898323587E-6	8.333957608491229E-5	0.0	3.703981159329435E-5	9.259952898323587E-6
6	9.259952898323587E-6	8.333957608491229E-5	0.0	3.703981159329435E-5	1.8519905796647174E-5
7	9.259952898323587E-6	8.333957608491229E-5	0.0	3.703981159329435E-5	1.8519905796647174E-5
8	9.259952898323587E-6	1.0185948188155946E-4	0.0	3.703981159329435E-5	3.703981159329435E-5
9	9.259952898323587E-6	2.5001872825473686E-4	0.0	5.555971738994153E-5	4.629976449161794E-5
10-11	9.259952898323587E-6	2.5001872825473686E-4	0.0	8.796955253407407E-5	4.629976449161794E-5
12-13	9.259952898323587E-6	2.5001872825473686E-4	0.0	1.0648945833072125E-4	4.629976449161794E-5
14-15	9.259952898323587E-6	3.796580688312671E-4	0.0	1.2037938767820664E-4	6.481967028826511E-5
16-17	9.259952898323587E-6	3.8891802172959065E-4	0.0	1.435292699240156E-4	7.87095996357505E-5
18-19	9.259952898323587E-6	4.1206790397539965E-4	0.0	1.57419199271501E-4	1.0185948188155946E-4
20-21	9.259952898323587E-6	4.583676684670176E-4	0.0	1.7593910506814815E-4	1.0185948188155946E-4
22-23	9.259952898323587E-6	5.231873387552827E-4	0.0	2.0834894021228072E-4	1.2963934057653022E-4
24-25	9.259952898323587E-6	5.741170796960623E-4	0.0	2.777985869497076E-4	1.527892228223392E-4
26-27	9.259952898323587E-6	6.111568912893567E-4	0.0	4.954074800603119E-4	1.57419199271501E-4
28-29	9.259952898323587E-6	6.111568912893567E-4	0.0	0.0015001123695284212	1.57419199271501E-4
30-31	9.259952898323587E-6	6.435667264334894E-4	0.0	0.0037502809238210526	1.6667915216982457E-4
32-33	9.259952898323587E-6	6.667166086792983E-4	0.0	0.007926519680964991	2.0371896376311893E-4
34-35	9.259952898323587E-6	7.917259728066667E-4	0.0	0.013612130760535673	2.222388695597661E-4
36-37	9.259952898323587E-6	9.167353369340352E-4	0.0	0.021311781595491734	2.222388695597661E-4
38-39	9.259952898323587E-6	9.398852191798441E-4	0.0	0.037294460297998246	2.361287989072515E-4
40-41	9.259952898323587E-6	0.0010463746775105653	0.0	0.0717646349620078	2.5001872825473686E-4
42-43	9.259952898323587E-6	0.001055634630408889	0.0	0.08598329263738366	2.5001872825473686E-4
44-45	9.259952898323587E-6	0.0011482341593921246	0.0	0.10400779095397053	2.5927868115306044E-4
46-47	9.259952898323587E-6	0.001231573735477037	0.0	0.12540754210199634	2.91688516297193E-4
48-49	9.259952898323587E-6	0.0012454636648245223	0.0	0.14409412705081337	3.0557844564467837E-4
50-51	9.259952898323587E-6	0.0013704730289518909	0.0	0.16371596724236104	3.0557844564467837E-4
52-53	9.259952898323587E-6	0.0013797329818502145	0.0	0.19016702269642236	3.194683749921638E-4
54-55	9.259952898323587E-6	0.001388992934748538	0.0	0.2450924333128287	3.3798828078881093E-4
56-57	9.259952898323587E-6	0.0014260327463418326	0.0	0.30479597962477	3.426182572379727E-4
58-59	9.259952898323587E-6	0.0014352926992401561	0.0	0.363087383119717	3.518782101362963E-4
60-61	9.259952898323587E-6	0.0014769624872826123	0.0	0.4024329229846939	3.611381630346199E-4
62-63	9.259952898323587E-6	0.0015047423459775828	0.0	0.41971199509296575	3.796580688312671E-4
64-65	9.259952898323587E-6	0.0016760514745965693	0.0	0.4351946363389628	3.889180217295907E-4
66-67	9.259952898323587E-6	0.0016853114274948929	0.0	0.442125711083358	4.0280795107707607E-4
68-69	9.259952898323587E-6	0.0017501310977831581	0.0	0.44834839943103144	4.0743792752623786E-4
70-71	9.259952898323587E-6	0.0017779109564781289	0.0	0.45457571775515404	4.0743792752623786E-4
72-73	9.259952898323587E-6	0.0017871709093764524	0.0	0.45941404314452816	4.25957833322885E-4
74-75	9.259952898323587E-6	0.001865880509012203	0.0	0.46350231234913797	4.25957833322885E-4
76-77	9.259952898323587E-6	0.0019214402264021444	0.0	0.4672618532258574	4.25957833322885E-4
78-79	9.259952898323587E-6	0.0019399601321987915	0.0	0.47081304516236444	4.25957833322885E-4
80-81	9.259952898323587E-6	0.0019445901086479534	0.0	0.4724150170137744	4.537376920178558E-4
82-83	9.259952898323587E-6	0.0019445901086479534	0.0	0.47303543385796215	4.8151755071282657E-4
84-85	9.259952898323587E-6	0.0019631100144446006	0.0	0.47357714110251403	4.8151755071282657E-4
86-87	9.259952898323587E-6	0.001972369967342924	0.0	0.4737623401604806	4.8151755071282657E-4
88-89	9.259952898323587E-6	0.0020325596611820273	0.0	0.47393364928909953	4.954074800603119E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12135	0.0	45.667236	1
GTATCAA	21360	0.0	35.977787	1
TCAACGC	25495	0.0	29.495152	4
ATCAACG	25635	0.0	29.285282	3
CAACGCA	26005	0.0	29.014097	5
TATCAAC	26840	0.0	28.285612	2
AACGCAG	27010	0.0	28.075764	6
CTTATAC	14095	0.0	25.821405	1
ACGCAGA	31250	0.0	24.117655	7
CGCAGAG	31735	0.0	23.789028	8
TATACAC	18110	0.0	22.524162	3
GTACATG	31740	0.0	21.60159	1
TACATGG	31395	0.0	21.20615	2
ACATGGG	31775	0.0	20.40702	3
TTATACA	18840	0.0	20.395048	2
GCAGAGT	38350	0.0	19.586454	9
GAGTACT	20540	0.0	18.181799	12-13
AGAGTAC	33645	0.0	17.815147	10-11
CATGGGG	23270	0.0	17.619967	4
CAGAGTA	36805	0.0	17.514462	10-11
>>END_MODULE