FastQCFastQC Report
Fri 27 May 2016
SRR2138800_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138800_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9864534
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106400.043.8600731
GTATCAA179750.033.9074751
TCAACGC201050.029.2278824
ATCAACG203100.028.9749643
CAACGCA206750.028.4627135
AACGCAG214400.027.5259196
TATCAAC221400.027.0924032
ACGCAGA250750.023.5524947
CGCAGAG254000.023.1849848
GTACATG251900.020.2269121
TACATGG248200.019.9361112
ACATGGG247450.019.1986123
CTTATAC66750.019.0672591
GCAGAGT310550.018.8685029
GAGTACT172300.017.8086412-13
CAGAGTA294150.017.61437410-11
CATGGGG180550.017.1689054
AGAGTAC266950.017.14329110-11
TATGCCG43300.015.86330542-43
GTACTTT199650.015.41127414-15
Produced by FastQC (version 0.11.5)