##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138800_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9864534
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.41662951336576	33.0	31.0	34.0	31.0	34.0
2	32.61492909852609	34.0	31.0	34.0	31.0	34.0
3	32.69647811036994	34.0	31.0	34.0	31.0	34.0
4	36.16417957503111	37.0	35.0	37.0	35.0	37.0
5	35.974750353133764	37.0	35.0	37.0	35.0	37.0
6	36.04144220091897	37.0	35.0	37.0	35.0	37.0
7	36.04280516444061	37.0	35.0	37.0	35.0	37.0
8	36.028499268186415	37.0	35.0	37.0	35.0	37.0
9	37.89118492571469	39.0	38.0	39.0	35.0	39.0
10-11	37.76451056887228	39.0	37.5	39.0	35.0	39.0
12-13	37.77407315946196	39.0	37.5	39.0	35.0	39.0
14-15	39.095192535197306	40.0	38.5	41.0	36.0	41.0
16-17	39.06652524082739	40.0	38.0	41.0	36.0	41.0
18-19	39.13109666406948	40.0	38.5	41.0	36.0	41.0
20-21	39.15024112917837	40.0	39.0	41.0	36.0	41.0
22-23	39.11092871437194	40.0	39.0	41.0	36.0	41.0
24-25	39.08379840303827	40.0	39.0	41.0	36.0	41.0
26-27	38.994670050357556	40.0	39.0	41.0	36.0	41.0
28-29	38.932570747378534	40.0	38.0	41.0	35.0	41.0
30-31	38.80075506897499	40.0	38.0	41.0	35.0	41.0
32-33	38.671041950181625	40.0	38.0	41.0	35.0	41.0
34-35	38.63317222622354	40.0	38.0	41.0	35.0	41.0
36-37	38.58773685781735	40.0	38.0	41.0	35.0	41.0
38-39	38.515644007618235	40.0	38.0	41.0	34.5	41.0
40-41	38.43730201232707	40.0	38.0	41.0	34.0	41.0
42-43	38.46245836517573	40.0	38.0	41.0	34.0	41.0
44-45	38.407687967399184	40.0	38.0	41.0	34.0	41.0
46-47	38.36295148086025	40.0	38.0	41.0	34.0	41.0
48-49	38.32468306625792	40.0	38.0	41.0	34.0	41.0
50-51	38.2652212553146	40.0	38.0	41.0	34.0	41.0
52-53	38.01946414199482	40.0	37.0	41.0	33.0	41.0
54-55	37.87395628892121	40.0	37.0	41.0	33.0	41.0
56-57	37.780057503551134	39.0	37.0	41.0	33.0	41.0
58-59	37.654017902441346	39.0	36.0	41.0	33.0	41.0
60-61	37.469451487071865	39.0	36.0	41.0	33.0	41.0
62-63	37.2257165050352	39.0	35.5	40.0	32.0	41.0
64-65	36.99414162508185	38.5	35.0	40.0	32.0	41.0
66-67	36.73775348115755	38.0	35.0	40.0	31.5	41.0
68-69	36.50124308875651	37.5	35.0	40.0	31.0	41.0
70-71	36.18931049531496	37.0	35.0	39.5	31.0	41.0
72-73	35.899981590925016	36.5	34.5	39.0	31.0	41.0
74-75	35.44801449020257	36.0	34.0	39.0	30.5	40.0
76-77	33.51561972764138	34.5	32.0	36.5	27.5	39.0
78-79	34.70197666586764	35.0	34.0	37.0	30.0	39.0
80-81	34.67309773769773	35.0	34.0	37.0	31.0	39.0
82-83	34.399081110981335	35.0	34.0	36.5	30.0	39.0
84-85	34.11577796275144	35.0	34.0	36.0	30.0	37.0
86-87	33.88104434917483	35.0	34.0	36.0	30.0	37.0
88-89	33.73955446354162	35.0	34.0	35.0	30.0	36.5
90-91	33.57413458633318	35.0	33.5	35.0	30.0	36.0
92-93	33.5353389665126	35.0	33.0	35.0	30.0	36.0
94-95	33.402242615825	35.0	33.0	35.0	30.0	36.0
96-97	33.42597564431284	35.0	33.0	35.0	30.0	35.5
98-99	33.52460976392252	35.0	33.0	35.0	30.0	35.0
100-101	32.313355585901974	34.0	31.0	34.5	27.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	5.0
20	29.0
21	174.0
22	723.0
23	2593.0
24	7048.0
25	14831.0
26	28301.0
27	46404.0
28	69451.0
29	97270.0
30	131577.0
31	173564.0
32	228798.0
33	312548.0
34	458001.0
35	715265.0
36	1279380.0
37	2363686.0
38	2782813.0
39	1134669.0
40	17402.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.397799429755125	20.493598582558487	14.110681761551028	23.997920226135367
2	14.53165990060859	21.197161438760702	42.043223159078366	22.227955501552344
3	18.213274930360253	26.21705582270194	30.516634770625256	25.053034476312554
4	12.463539687239882	18.15704061019659	39.12890403997448	30.250515662589045
5	13.149440174135608	37.88452040529997	35.94846609497333	13.017573325591089
6	29.763839553630806	38.3546522472548	18.911366843462606	12.970141355651792
7	26.307253844935808	32.58220814080016	23.483390092223313	17.627147922040717
8	23.799735496882064	37.767126151118745	21.380209141151525	17.05292921084767
9	25.21945790850333	17.144783524492897	21.375931189451016	36.25982737755275
10-11	23.926857568740704	27.03392273775933	29.31120719944804	19.728012494051924
12-13	24.769452030339707	24.389947197167846	29.38889645253812	21.451704319954327
14-15	21.86227327623641	25.932518620264304	26.717189480675284	25.488018622824
16-17	21.646117947505626	29.071174645629128	27.43006150119357	21.85264590567168
18-19	21.71007267043735	28.331637358642585	28.887492303235003	21.070797667685063
20-21	22.378547419385626	27.54173272816364	28.74022459227813	21.3394952601726
22-23	22.076843517336684	27.553627491519308	28.609106696049942	21.760422295094067
24-25	21.909214054561126	27.67622515719208	28.682656022261742	21.73190476598505
26-27	21.933885577617566	27.822271533309912	28.589381173067967	21.65446171600455
28-29	21.845416198089424	27.79661447020661	28.67127902303167	21.6866903086723
30-31	22.126872126343613	27.7258864923133	28.523822572051927	21.62341880929116
32-33	21.934794870370833	27.78766526871755	28.503054818593554	21.774485042318066
34-35	21.933229986312696	27.914185337077	28.53599530721031	21.616589369399993
36-37	22.032700446050583	27.80837365011853	28.468355935472257	21.69056996835863
38-39	21.821954201071208	27.853397292220894	28.59603621440554	21.72861229230236
40-41	22.080678973939758	27.733374488576008	28.44418328030739	21.74176325717685
42-43	21.996659954912648	27.80153033035406	28.331066076065937	21.870743638667353
44-45	21.992981908283873	27.929301235646832	28.216997066504913	21.86071978956438
46-47	22.129607830019648	27.854171115931326	27.954493993171077	22.06172706087795
48-49	22.059315427027844	27.896047931701982	27.955019874926386	22.089616766343788
50-51	21.999941226420727	28.01602997386874	27.950114912197797	22.033913887512732
52-53	22.18124283493689	27.96742367959691	27.871614221981524	21.979719263484682
54-55	22.14604972483375	27.874118668001596	27.837356592632982	22.14247501453167
56-57	22.175184358216228	28.030766350773206	27.76841622538263	22.025633065627936
58-59	22.06983574263017	28.08856336935729	27.859354189164193	21.98224669884835
60-61	22.22097811230511	28.142037034157152	27.697319576703123	21.939665276834617
62-63	22.128839062279262	28.21144102780368	27.667091878477702	21.992628031439352
64-65	22.117145843214296	28.145401190943254	27.6743413569481	22.06311160889435
66-67	22.213250276022848	28.09179093129814	27.591527997088157	22.103430795590853
68-69	22.13427166904737	28.04858383064865	27.67848417869653	22.138660321607453
70-71	22.16886644127555	27.85913512639745	27.7372104265504	22.2347880057766
72-73	22.201308383104752	27.93929691856889	27.685331697252767	22.174063001073584
74-75	22.123581837281023	28.043667271890904	27.608280316397664	22.22447057443041
76-77	22.202684395518492	27.99413528436578	27.604732815413946	22.19844750470178
78-79	22.165480051668514	27.979740208429725	27.57524460317941	22.27953513672235
80-81	22.177223598655782	28.005081964073792	27.574778987095566	22.24291545017486
82-83	22.20103940257809	27.98827585971918	27.644090714070984	22.166594023631745
84-85	22.280726952360133	27.936251522942573	27.620919069513185	22.162102455184108
86-87	22.15509607999738	27.96219755358705	27.67154853194999	22.211157834465574
88-89	22.18111728649945	27.971785038189317	27.596807776208447	22.250289899102786
90-91	22.207713934401195	27.959152873850268	27.654365963483063	22.178767228265475
92-93	22.197171019318045	27.96318514211401	27.63346140444023	22.206182434127715
94-95	22.097184495629605	28.00170900066786	27.672949061801887	22.22815744190065
96-97	22.114947120530747	27.995989694946704	27.705313238974753	22.18374994554779
98-99	22.373669010757293	28.553910286501655	27.7896501486515	21.28277055408955
100-101	26.37068918495017	35.63828310362733	28.37461648113553	9.616411230286968
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2640.0
1	2126.5
2	2360.5
3	3272.0
4	4258.0
5	6651.0
6	8773.5
7	10468.0
8	11676.5
9	11539.5
10	10492.0
11	9429.0
12	10173.5
13	13334.0
14	20389.0
15	29909.5
16	37698.5
17	42584.0
18	44459.0
19	43696.5
20	41915.5
21	40722.5
22	42287.0
23	48396.0
24	59032.5
25	74551.0
26	94576.5
27	116375.0
28	139158.0
29	162460.5
30	185009.5
31	207270.0
32	228423.5
33	247358.0
34	266741.0
35	286504.5
36	307328.5
37	326640.5
38	342784.0
39	357579.5
40	371808.0
41	385838.0
42	397268.5
43	407710.5
44	417059.0
45	424724.5
46	432544.0
47	438491.0
48	436281.5
49	426764.0
50	415549.0
51	399558.0
52	377091.5
53	353380.0
54	329259.0
55	303650.5
56	276405.0
57	247269.5
58	218315.0
59	188576.0
60	156939.0
61	127217.5
62	101530.0
63	79087.0
64	59581.0
65	44600.0
66	33686.0
67	25911.0
68	19926.5
69	14511.0
70	10481.0
71	7589.0
72	5350.0
73	3729.0
74	2605.5
75	1799.0
76	1232.5
77	852.5
78	586.5
79	391.5
80	265.0
81	176.0
82	123.5
83	89.5
84	73.0
85	58.5
86	47.5
87	40.5
88	30.0
89	19.5
90	12.5
91	10.0
92	8.0
93	4.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0010137326304516768
3	0.0019869159556852862
4	4.156303784851874E-4
5	3.852183995716371E-4
6	5.474156204439054E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0010238699567561933
14-15	8.059174412090829E-4
16-17	1.0137326304516766E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.5645870813665034E-5
28-29	2.131429851446444E-4
30-31	3.0491061596111536E-5
32-33	0.0011973543055459669
34-35	0.002044746726168796
36-37	0.0012172571939129745
38-39	7.654733058511176E-4
40-41	3.127297097446109E-5
42-43	4.203163258636817E-5
44-45	7.106824105095715E-4
46-47	5.360559543782075E-6
48-49	0.0
50-51	0.0
52-53	0.0010108944593040406
54-55	0.003641498945805573
56-57	0.006260261673303145
58-59	0.004665197727484782
60-61	2.5918889427425815E-4
62-63	2.9116111149706135E-5
64-65	1.0597245446216448E-4
66-67	1.9655253992339455E-4
68-69	6.02836853779992E-6
70-71	8.539845608131251E-5
72-73	6.171094076971563E-6
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	4.0312846501513446E-5
88-89	3.677569502488182E-4
90-91	1.5202352992555547E-4
92-93	4.3512872827242594E-4
94-95	2.6408644220175575E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	188.0
20-21	633.0
22-23	1430.0
24-25	2869.0
26-27	4874.0
28-29	9875.0
30-31	24588.0
32-33	29935.0
34-35	40554.0
36-37	63636.0
38-39	74623.0
40-41	74734.0
42-43	84972.0
44-45	99069.0
46-47	99715.0
48-49	94048.0
50-51	86561.0
52-53	83841.0
54-55	91355.0
56-57	95185.0
58-59	96632.0
60-61	95699.0
62-63	93044.0
64-65	95941.0
66-67	102130.0
68-69	97288.0
70-71	95902.0
72-73	91724.0
74-75	92852.0
76-77	90486.0
78-79	97241.0
80-81	98097.0
82-83	94579.0
84-85	94170.0
86-87	98555.0
88-89	104687.0
90-91	110680.0
92-93	115053.0
94-95	134314.0
96-97	201383.0
98-99	817729.0
100-101	5883663.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.2945290092683
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.86957711992014	79.32416935954812
2	6.595237135704311	11.514562389314872
3	1.4957711356893733	3.917179103869866
4	0.5047517121142373	1.7624825190253657
5	0.2246769463425574	0.9806534105107095
6	0.11068886844081448	0.5797519582265857
7	0.05929076661606676	0.36230316824435976
8	0.04130036667727107	0.28842368456019657
9	0.02313099380434895	0.18172882887002503
>10	0.07410834066081806	0.9820752427578211
>50	0.0012933384511977031	0.07346058222304766
>100	1.5623689878898868E-4	0.02575796128726416
>500	1.703867999688407E-5	0.007451791561790856
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0137326304516767E-5
2	0.0	1.0137326304516767E-5	0.0	0.0	1.0137326304516767E-5
3	0.0	1.0137326304516767E-5	0.0	0.0	1.0137326304516767E-5
4	0.0	4.054930521806707E-5	0.0	1.0137326304516767E-5	1.0137326304516767E-5
5	0.0	5.0686631522583836E-5	0.0	1.0137326304516767E-5	1.0137326304516767E-5
6	0.0	5.0686631522583836E-5	0.0	1.0137326304516767E-5	1.0137326304516767E-5
7	0.0	6.08239578271006E-5	0.0	3.04119789135503E-5	1.0137326304516767E-5
8	0.0	6.08239578271006E-5	0.0	3.04119789135503E-5	2.0274652609033534E-5
9	0.0	2.128838523948521E-4	0.0	4.054930521806707E-5	4.054930521806707E-5
10-11	0.0	2.128838523948521E-4	0.0	4.054930521806707E-5	4.054930521806707E-5
12-13	0.0	2.3315850500388563E-4	0.0	5.0686631522583836E-5	4.054930521806707E-5
14-15	0.0	3.3960043120131165E-4	0.0	7.096128413161736E-5	4.054930521806707E-5
16-17	0.0	3.4466909435357007E-4	0.0	7.096128413161736E-5	4.054930521806707E-5
18-19	0.0	3.497377575058285E-4	0.0	7.096128413161736E-5	4.054930521806707E-5
20-21	0.0	3.5480642065808686E-4	0.0	8.616727358839252E-5	4.561796837032545E-5
22-23	0.0	4.2069904163744584E-4	0.0	1.1151058934968445E-4	6.08239578271006E-5
24-25	0.0	4.612483468555129E-4	0.0	1.875405366335602E-4	8.616727358839252E-5
26-27	0.0	4.865916626168048E-4	0.0	2.2302117869936887E-4	9.12359367406509E-5
28-29	0.0	4.865916626168048E-4	0.0	4.3590503109422094E-4	9.12359367406509E-5
30-31	0.0	5.271409678348719E-4	0.0	0.0014040196931755721	9.630459989290929E-5
32-33	0.0	5.474156204439054E-4	0.0	0.0031222965017911643	1.1151058934968443E-4
34-35	0.0	6.183769045755228E-4	0.0	0.005403194920307436	1.1151058934968443E-4
36-37	0.0	7.045441781639154E-4	0.0	0.009118525010912832	1.1151058934968443E-4
38-39	0.0	7.197501676206905E-4	0.0	0.014638299183722212	1.216479156542012E-4
40-41	0.0	7.805741254477911E-4	0.0	0.02074603828219356	1.3178524195871797E-4
42-43	0.0	8.059174412090829E-4	0.0	0.026027585286846802	1.3178524195871797E-4
44-45	0.0	8.667413990361835E-4	0.0	0.031942715185532336	1.3178524195871797E-4
46-47	0.0	9.427713463200593E-4	0.0	0.03815182754704885	1.3178524195871797E-4
48-49	0.0	9.579773357768346E-4	0.0	0.044142987393018265	1.5205989456775151E-4
50-51	0.0	0.0010542819356697437	0.0	0.05033182510192574	1.5205989456775151E-4
52-53	0.0	0.0010644192619742605	0.0	0.05639901489517903	1.6726588402452664E-4
54-55	0.0	0.0010694879251265188	0.0	0.06291224704583105	1.824718734813018E-4
56-57	0.0	0.0011049685671923276	0.0	0.06889833822864821	1.824718734813018E-4
58-59	0.0	0.0011049685671923276	0.0	0.0747830561484202	1.824718734813018E-4
60-61	0.0	0.001125243219801361	0.0	0.08097696252047994	2.0274652609033534E-4
62-63	0.0	0.0011607238618671699	0.0	0.08678565049296805	2.3315850500388563E-4
64-65	0.0	0.0012975777669781462	0.0	0.09223953204479807	2.3822716815614402E-4
66-67	0.0	0.0013026464301304046	0.0	0.09801780803837262	2.432958313084024E-4
68-69	0.0	0.0013584017248052468	0.0	0.10390759462129687	2.432958313084024E-4
70-71	0.0	0.0013685390511097636	0.0	0.10945778077301979	2.5343315761291915E-4
72-73	0.0	0.0013685390511097636	0.0	0.11471398446191172	2.9398246283098624E-4
74-75	0.0	0.001388813703718797	0.0	0.11882467027839327	2.9398246283098624E-4
76-77	0.0	0.0014496376615458976	0.0	0.12246397042171481	2.9398246283098624E-4
78-79	0.0	0.0015003242930684815	0.0	0.12578394478644406	2.9398246283098624E-4
80-81	0.0	0.0015053929562207399	0.0	0.12738564234255767	3.04119789135503E-4
82-83	0.0	0.0015104616193729983	0.0	0.12800401924713323	3.2439444174453655E-4
84-85	0.0	0.001520598945677515	0.0	0.12849567957290228	3.2439444174453655E-4
86-87	0.0	0.0015814229035046156	0.0	0.12868322010953584	3.2439444174453655E-4
88-89	1.0137326304516767E-5	0.0016878648297020417	0.0	0.12876431871997196	3.2439444174453655E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10640	0.0	43.860073	1
GTATCAA	17975	0.0	33.907475	1
TCAACGC	20105	0.0	29.227882	4
ATCAACG	20310	0.0	28.974964	3
CAACGCA	20675	0.0	28.462713	5
AACGCAG	21440	0.0	27.525919	6
TATCAAC	22140	0.0	27.092403	2
ACGCAGA	25075	0.0	23.552494	7
CGCAGAG	25400	0.0	23.184984	8
GTACATG	25190	0.0	20.226912	1
TACATGG	24820	0.0	19.936111	2
ACATGGG	24745	0.0	19.198612	3
CTTATAC	6675	0.0	19.067259	1
GCAGAGT	31055	0.0	18.868502	9
GAGTACT	17230	0.0	17.80864	12-13
CAGAGTA	29415	0.0	17.614374	10-11
CATGGGG	18055	0.0	17.168905	4
AGAGTAC	26695	0.0	17.143291	10-11
TATGCCG	4330	0.0	15.863305	42-43
GTACTTT	19965	0.0	15.411274	14-15
>>END_MODULE