FastQCFastQC Report
Fri 27 May 2016
SRR2138799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10579599
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111900.043.8472181
GTATCAA200450.034.9114721
TCAACGC234550.028.9134834
ATCAACG236100.028.7061923
CAACGCA240050.028.2333015
AACGCAG249800.027.2317056
TATCAAC256100.026.935622
ACGCAGA290850.023.3454997
CGCAGAG293250.023.1118978
GTACATG285200.019.1573721
GCAGAGT356550.018.9271019
TACATGG281950.018.832542
ACATGGG283450.018.0138233
AGAGTAC315800.017.57083910-11
GAGTACT198300.017.49788512-13
CAGAGTA343350.016.77253710-11
AGTACTT218950.015.70516512-13
CATGGGG200500.015.5327864
GTACTTT231700.015.0383414-15
GTGGTAT63950.014.4345271
Produced by FastQC (version 0.11.5)