##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138799_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10579599
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.426278065926695	33.0	31.0	34.0	31.0	34.0
2	32.62838667136628	34.0	31.0	34.0	31.0	34.0
3	32.712565570774466	34.0	31.0	34.0	31.0	34.0
4	36.179241859734006	37.0	35.0	37.0	35.0	37.0
5	35.989235319788584	37.0	35.0	37.0	35.0	37.0
6	36.05312980198966	37.0	35.0	37.0	35.0	37.0
7	36.05529113154478	37.0	35.0	37.0	35.0	37.0
8	36.04176897441954	37.0	35.0	37.0	35.0	37.0
9	37.910973657886274	39.0	38.0	39.0	35.0	39.0
10-11	37.78691040180256	39.0	37.5	39.0	35.0	39.0
12-13	37.79580714732194	39.0	38.0	39.0	35.0	39.0
14-15	39.11716152946818	40.0	38.5	41.0	36.0	41.0
16-17	39.09356049317181	40.0	38.0	41.0	36.0	41.0
18-19	39.16413438732413	40.0	39.0	41.0	36.0	41.0
20-21	39.18184529364284	40.0	39.0	41.0	36.0	41.0
22-23	39.14504909832755	40.0	39.0	41.0	36.0	41.0
24-25	39.117063803434235	40.0	39.0	41.0	36.0	41.0
26-27	39.03037843595713	40.0	39.0	41.0	36.0	41.0
28-29	38.966751832122654	40.0	38.5	41.0	35.0	41.0
30-31	38.836381533799056	40.0	38.0	41.0	35.0	41.0
32-33	38.706418519773415	40.0	38.0	41.0	35.0	41.0
34-35	38.66946899572969	40.0	38.0	41.0	35.0	41.0
36-37	38.6264381154876	40.0	38.0	41.0	35.0	41.0
38-39	38.56124819998777	40.0	38.0	41.0	35.0	41.0
40-41	38.48021894664953	40.0	38.0	41.0	34.0	41.0
42-43	38.50418638751924	40.0	38.0	41.0	34.0	41.0
44-45	38.44979475252342	40.0	38.0	41.0	34.0	41.0
46-47	38.40828823576639	40.0	38.0	41.0	34.0	41.0
48-49	38.36972368580057	40.0	38.0	41.0	34.0	41.0
50-51	38.31691706267628	40.0	38.0	41.0	34.0	41.0
52-53	38.071657492863935	40.0	37.0	41.0	33.0	41.0
54-55	37.92983861614812	40.0	37.0	41.0	33.0	41.0
56-57	37.84345186139258	40.0	37.0	41.0	33.0	41.0
58-59	37.71785860584117	39.0	36.5	41.0	33.0	41.0
60-61	37.54353043813696	39.0	36.0	41.0	33.0	41.0
62-63	37.30098470661367	39.0	35.5	40.5	32.5	41.0
64-65	37.067312584560106	39.0	35.0	40.0	32.0	41.0
66-67	36.81257612900567	38.0	35.0	40.0	32.0	41.0
68-69	36.57865087434384	37.5	35.0	40.0	31.5	41.0
70-71	36.27153915179734	37.0	35.0	39.5	31.0	41.0
72-73	35.983342991620816	36.5	35.0	39.0	31.0	41.0
74-75	35.53160754515412	36.0	34.0	39.0	31.0	40.0
76-77	33.59681778503954	34.5	32.0	36.5	28.0	39.0
78-79	34.77704923306058	35.0	34.0	37.0	30.0	39.0
80-81	34.744364822590526	35.0	34.0	37.0	31.0	39.0
82-83	34.47060032485074	35.0	34.0	36.5	30.5	39.0
84-85	34.18401500286646	35.0	34.0	36.0	30.0	37.0
86-87	33.94686925153842	35.0	34.0	36.0	30.0	37.0
88-89	33.80542466045418	35.0	34.0	35.0	30.0	37.0
90-91	33.63450777399388	35.0	34.0	35.0	30.0	36.0
92-93	33.59352316938601	35.0	34.0	35.0	30.0	36.0
94-95	33.460854817071734	35.0	33.0	35.0	30.0	36.0
96-97	33.47881028079796	35.0	33.0	35.0	30.0	35.5
98-99	33.57074118227952	35.0	33.5	35.0	30.0	35.0
100-101	32.35597349106965	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	15.0
20	44.0
21	170.0
22	733.0
23	2565.0
24	6928.0
25	15352.0
26	28143.0
27	46482.0
28	70555.0
29	99219.0
30	133858.0
31	177601.0
32	236052.0
33	325257.0
34	478183.0
35	747109.0
36	1346276.0
37	2507388.0
38	3041504.0
39	1296126.0
40	20036.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.235296347243406	20.707448363591098	14.180216093256465	23.877039195909035
2	14.40248064887816	21.360972976958248	42.235391309032856	22.00115506513074
3	18.07920087551461	26.554254890977525	30.521000383954522	24.845543849553348
4	12.362621574329914	18.249316416145284	39.49275280125707	29.895309208267733
5	12.981183445409439	38.08994262241546	35.99247492832174	12.93639900385337
6	29.42898159979145	38.613584277749325	19.12913342093627	12.828300701522958
7	26.052745477404198	32.747866908755235	23.8987413417087	17.300646272131864
8	23.67667243342588	37.833381019450734	21.60687753855321	16.883069008570175
9	25.38044211316516	17.05795276361609	21.71079452066189	35.85081060255686
10-11	23.858249441129548	27.158885093796332	29.429136653909882	19.55372881116424
12-13	24.623837107498304	24.63788314987699	29.561961502406657	21.176318240218045
14-15	21.77456066059708	26.05025558815523	27.058651999334565	25.11653175191313
16-17	21.414153835625584	29.133152629954417	27.74462929049813	21.708064243921868
18-19	21.472784554499654	28.491467398717095	29.23941162609282	20.796336420690427
20-21	22.21475639049359	27.74861550490826	29.02131216900009	21.015315935598057
22-23	21.927412840532952	27.6324992638339	28.898615156939268	21.541472738693884
24-25	21.670221383661474	27.729118509765566	29.00615974976179	21.59450035681117
26-27	21.68848372489656	27.89315927293508	28.8613577847951	21.556999217373264
28-29	21.593841161230458	28.06987439992474	28.854981346345433	21.481303092499374
30-31	21.927624743388726	27.854697044423933	28.734121192467864	21.48355701971948
32-33	21.69334173291286	28.036363150652306	28.697257253347953	21.573037863086885
34-35	21.814160345352263	28.058015600311748	28.71508023297567	21.41274382136032
36-37	21.823730747044152	27.981986335959945	28.689367302875862	21.504915614120033
38-39	21.65751657689103	28.11415685607223	28.724735243586437	21.503591323450305
40-41	21.927731798482483	27.95165968568471	28.592344288907455	21.528264226925348
42-43	21.77480056228278	28.052477431255085	28.44006363672712	21.73265836973501
44-45	21.87954260843104	28.183608561925265	28.297508249304858	21.639340580338835
46-47	21.904180761053656	28.122890115771238	28.100309263650026	21.872619859525077
48-49	21.872708271101406	28.21400607030912	28.081497847555315	21.831787811034157
50-51	21.868880219377377	28.290924286092782	27.982878129113768	21.85731736541607
52-53	22.016922336465406	28.192759123601974	27.873767000040537	21.916551539892076
54-55	22.003784276690645	28.1196674298736	27.924565938967742	21.951982354468004
56-57	22.005934393429623	28.183716030802756	27.90124145301018	21.90910812275744
58-59	21.96632969711859	28.281175889442224	27.889360330660136	21.863134082779055
60-61	22.08645458755192	28.227790117764023	27.853864795622975	21.831890499061082
62-63	22.050320598019983	28.257811157037654	27.78462099530175	21.907247249640612
64-65	22.009129378122147	28.197050696820476	27.821269896207625	21.972550028849753
66-67	22.077528981758263	28.20490860224795	27.672434526216467	22.045127889777316
68-69	21.984552984028248	28.231677891657803	27.77076860755224	22.01300051676171
70-71	22.02328066364226	28.00818795903043	27.806582176329087	22.161949200998222
72-73	22.081588102138365	28.045521458366114	27.772816543073482	22.100073896422042
74-75	21.974679242177192	28.177086849587525	27.735021770427092	22.11321213780819
76-77	22.01481643030619	28.149839532110203	27.718138518554692	22.117205519028918
78-79	22.027287964086007	28.130018423314336	27.674642496793005	22.168051115806655
80-81	22.0112164526838	28.172803378038996	27.690661394222353	22.12531877505485
82-83	22.065903790031097	28.085360175255154	27.793609194134895	22.055126840578858
84-85	22.196614131734346	28.113593730573093	27.671326374144062	22.018465763548495
86-87	22.030733289206506	28.129577811339228	27.754677048645664	22.085011850808602
88-89	22.06156720056353	28.08282599826458	27.70490871869173	22.15069808248016
90-91	22.08666573968692	28.102955758108994	27.738535195057267	22.071843307146814
92-93	22.11605003956822	28.10556822001003	27.692410525897586	22.085971214524168
94-95	21.99478126865729	28.124813234974805	27.778136366626153	22.102269129741746
96-97	21.979340569068416	28.106253245519337	27.82050802684423	22.093898158568017
98-99	22.23316645878275	28.711077053034913	27.873551972021293	21.18220451616104
100-101	26.244414147193574	35.75262356427885	28.450707648916897	9.552254639610684
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2446.0
1	2173.5
2	2908.0
3	4159.0
4	5737.5
5	9277.5
6	12206.0
7	14188.5
8	15296.0
9	14653.0
10	12956.0
11	11500.5
12	12362.5
13	16257.5
14	25197.0
15	36773.5
16	45671.5
17	50907.0
18	52574.5
19	51122.5
20	48732.0
21	47441.5
22	49309.0
23	55993.0
24	68408.0
25	86907.5
26	110147.5
27	134761.0
28	160641.5
29	187618.5
30	211703.5
31	233860.0
32	257038.0
33	279849.5
34	302427.5
35	324370.5
36	347018.0
37	367871.5
38	384192.5
39	399371.0
40	412986.5
41	424728.5
42	433478.5
43	442202.0
44	450799.0
45	456404.5
46	459353.5
47	459152.0
48	455108.5
49	446382.0
50	435012.0
51	418520.0
52	396296.0
53	373369.0
54	349200.0
55	320679.5
56	290902.5
57	259987.0
58	228150.5
59	196481.0
60	163603.0
61	132716.0
62	105026.5
63	80718.0
64	60341.5
65	45025.0
66	33758.0
67	25427.0
68	19115.0
69	13632.0
70	9542.0
71	6693.0
72	4638.0
73	3203.0
74	2217.0
75	1507.5
76	1034.0
77	722.5
78	484.0
79	336.5
80	252.0
81	183.0
82	130.0
83	87.5
84	60.5
85	41.5
86	30.5
87	24.0
88	17.0
89	11.5
90	7.0
91	5.0
92	4.5
93	2.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0010302847962384964
3	0.002088926054758786
4	5.954857079176631E-4
5	3.024689310057971E-4
6	4.2534693422690217E-4
7	0.0
8	0.0
9	0.0
10-11	4.72607704696558E-6
12-13	0.0010066544110036684
14-15	7.467201734205615E-4
16-17	1.3705623436200182E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.512459521481547E-5
28-29	1.7509361474757676E-4
30-31	2.3697761779576784E-5
32-33	0.0012073497176394031
34-35	0.0018318512273474779
36-37	0.001261203370242314
38-39	6.9106650118443E-4
40-41	2.4373229010713545E-5
42-43	3.442586606134847E-5
44-45	6.559052491202671E-4
46-47	0.0
48-49	0.0
50-51	0.0
52-53	9.251637149959371E-4
54-55	0.0033822680291954858
56-57	0.006516626000158693
58-59	0.0046262594870558225
60-61	2.3376393919419396E-4
62-63	2.7495062849039514E-5
64-65	8.89737104928921E-5
66-67	1.800682661305434E-4
68-69	1.1394964747397561E-5
70-71	1.67355314549796E-4
72-73	1.75331562211817E-5
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86-87	2.563029049243093E-5
88-89	3.575363560843684E-4
90-91	1.6506022354304147E-4
92-93	5.838559795073261E-4
94-95	3.551724550100661E-4
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	166.0
20-21	665.0
22-23	1553.0
24-25	3395.0
26-27	5656.0
28-29	11678.0
30-31	29483.0
32-33	35220.0
34-35	48208.0
36-37	74559.0
38-39	89866.0
40-41	88974.0
42-43	99429.0
44-45	116861.0
46-47	116932.0
48-49	110088.0
50-51	102525.0
52-53	98459.0
54-55	104597.0
56-57	108578.0
58-59	108331.0
60-61	106418.0
62-63	101088.0
64-65	104310.0
66-67	109133.0
68-69	111435.0
70-71	110260.0
72-73	105319.0
74-75	105781.0
76-77	104274.0
78-79	110347.0
80-81	111941.0
82-83	107380.0
84-85	106194.0
86-87	110259.0
88-89	117045.0
90-91	122085.0
92-93	125924.0
94-95	145983.0
96-97	212044.0
98-99	851435.0
100-101	6145721.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.90822790563392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.66305803883897	76.13131366677821
2	7.1317583778886915	12.110899314353741
3	1.7740113910951836	4.518844905069016
4	0.6766596475531933	2.2981588627596983
5	0.3089451634194058	1.3115993172979112
6	0.14977715379858478	0.7630387625872451
7	0.08787399254664935	0.5222857490290215
8	0.050466837612114256	0.34280397997168116
9	0.039118054292771806	0.29893002022040666
>10	0.11547561638752477	1.4823775157374393
>50	0.0023876802419946245	0.13667738241565774
>100	4.141544481741683E-4	0.05396832030289496
>500	5.389187677294037E-5	0.02910220347712199
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.45215409393116E-6	9.45215409393116E-6
2	0.0	3.780861637572464E-5	0.0	1.890430818786232E-5	9.45215409393116E-6
3	0.0	4.72607704696558E-5	0.0	1.890430818786232E-5	9.45215409393116E-6
4	0.0	1.0397369503324275E-4	0.0	5.6712924563586954E-5	9.45215409393116E-6
5	0.0	1.0397369503324275E-4	0.0	5.6712924563586954E-5	9.45215409393116E-6
6	0.0	1.0397369503324275E-4	0.0	5.6712924563586954E-5	3.780861637572464E-5
7	0.0	1.0397369503324275E-4	0.0	5.6712924563586954E-5	3.780861637572464E-5
8	0.0	1.2287800322110506E-4	0.0	5.6712924563586954E-5	4.72607704696558E-5
9	0.0	1.890430818786232E-4	0.0	7.561723275144928E-5	8.506938684538043E-5
10-11	0.0	1.890430818786232E-4	0.0	7.561723275144928E-5	8.506938684538043E-5
12-13	0.0	1.9376915892558877E-4	0.0	9.924761798627718E-5	8.506938684538043E-5
14-15	0.0	2.552081605361413E-4	0.0	1.0397369503324275E-4	1.1815192617413948E-4
16-17	0.0	2.646603146300725E-4	0.0	1.4650838845593298E-4	1.3705623436200182E-4
18-19	0.0	2.788385457709692E-4	0.0	1.7013877369076086E-4	1.5123446550289855E-4
20-21	0.0	3.213732391936594E-4	0.0	1.8431700483165762E-4	1.606866195968297E-4
22-23	0.0	3.733600867102808E-4	0.0	1.9849523597255435E-4	1.9376915892558877E-4
24-25	0.0	4.15894780132971E-4	0.0	2.2685169825434782E-4	2.079473900664855E-4
26-27	0.0	4.442512424147645E-4	0.0	2.835646228179348E-4	2.079473900664855E-4
28-29	0.0	4.4897731946173005E-4	0.0	7.41994096373596E-4	2.079473900664855E-4
30-31	0.0	5.482249374480073E-4	0.0	0.0023015995218722374	2.1267346711345108E-4
32-33	0.0	5.671292456358696E-4	0.0	0.005822526921861594	2.457560064422101E-4
34-35	0.0	6.711029406691123E-4	0.0	0.009797157718359647	2.552081605361413E-4
36-37	9.45215409393116E-6	7.750766357023551E-4	0.0	0.015416463327201722	2.552081605361413E-4
38-39	9.45215409393116E-6	8.223374061720109E-4	0.0	0.024471626949187773	2.6466031463007243E-4
40-41	9.45215409393116E-6	9.641197175809783E-4	0.0	0.034339675823251904	2.7411246872400364E-4
42-43	9.45215409393116E-6	9.924761798627718E-4	0.0	0.04259613242430077	2.835646228179348E-4
44-45	9.45215409393116E-6	0.0011200802601308423	0.0	0.05108416680065095	2.9774285395883153E-4
46-47	9.45215409393116E-6	0.0012193278781171195	0.0	0.06030001704223383	3.1664716214669384E-4
48-49	9.45215409393116E-6	0.0012193278781171195	0.0	0.0693788110494547	3.497297014754529E-4
50-51	9.45215409393116E-6	0.001441453499324502	0.0	0.07820712297318641	3.497297014754529E-4
52-53	9.45215409393116E-6	0.0014650838845593297	0.0	0.08617528887437038	3.8753831785117755E-4
54-55	9.45215409393116E-6	0.0014887142697941576	0.0	0.09433249785743297	4.3007301127386774E-4
56-57	9.45215409393116E-6	0.0015028925009350543	0.0	0.10246607645526074	4.442512424147645E-4
58-59	9.45215409393116E-6	0.0015028925009350543	0.0	0.10955991810275606	4.5370339650869563E-4
60-61	9.45215409393116E-6	0.0015312489632168477	0.0	0.11628985181763506	4.678816276495924E-4
62-63	9.45215409393116E-6	0.0015690575795925725	0.0	0.12257553429009926	4.915120128844202E-4
64-65	9.45215409393116E-6	0.0017581006614711956	0.0	0.12918259000175716	4.915120128844202E-4
66-67	1.4178231140896738E-5	0.0017628267385181612	0.0	0.1358936194084483	4.915120128844202E-4
68-69	1.890430818786232E-5	0.0018384439712696105	0.0	0.14299218713299058	4.915120128844202E-4
70-71	1.890430818786232E-5	0.0018620743565044384	0.0	0.14946691268733342	4.915120128844202E-4
72-73	1.890430818786232E-5	0.001880978664692301	0.0	0.154467102203023	5.482249374480073E-4
74-75	1.890430818786232E-5	0.00191406120402106	0.0	0.1584937198470377	5.482249374480073E-4
76-77	1.890430818786232E-5	0.0019849523597255435	0.0	0.1624730767205827	5.529510144949728E-4
78-79	1.890430818786232E-5	0.001994404513819475	0.0	0.16620195151063855	5.576770915419384E-4
80-81	1.890430818786232E-5	0.0020038566679134056	0.0	0.1680356694048612	5.671292456358696E-4
82-83	1.890430818786232E-5	0.002013308822007337	0.0	0.16879656780942265	6.143900161055254E-4
84-85	1.890430818786232E-5	0.002070021746570924	0.0	0.1695621922910311	6.19116093152491E-4
86-87	1.890430818786232E-5	0.0020983782088527174	0.0	0.16983630475975509	6.238421701994565E-4
88-89	1.890430818786232E-5	0.002268516982543478	0.0	0.16994027845478832	6.238421701994565E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11190	0.0	43.847218	1
GTATCAA	20045	0.0	34.911472	1
TCAACGC	23455	0.0	28.913483	4
ATCAACG	23610	0.0	28.706192	3
CAACGCA	24005	0.0	28.233301	5
AACGCAG	24980	0.0	27.231705	6
TATCAAC	25610	0.0	26.93562	2
ACGCAGA	29085	0.0	23.345499	7
CGCAGAG	29325	0.0	23.111897	8
GTACATG	28520	0.0	19.157372	1
GCAGAGT	35655	0.0	18.927101	9
TACATGG	28195	0.0	18.83254	2
ACATGGG	28345	0.0	18.013823	3
AGAGTAC	31580	0.0	17.570839	10-11
GAGTACT	19830	0.0	17.497885	12-13
CAGAGTA	34335	0.0	16.772537	10-11
AGTACTT	21895	0.0	15.705165	12-13
CATGGGG	20050	0.0	15.532786	4
GTACTTT	23170	0.0	15.03834	14-15
GTGGTAT	6395	0.0	14.434527	1
>>END_MODULE