FastQCFastQC Report
Fri 27 May 2016
SRR2138798_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138798_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9361189
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104100.042.351211
GTATCAA184000.034.0564421
TCAACGC219950.027.7387264
ATCAACG223500.027.2796653
CAACGCA223500.027.2427255
AACGCAG228900.026.6724596
TATCAAC234500.026.4406642
ACGCAGA267000.022.7890857
CGCAGAG272500.022.3442698
GTACATG273050.021.6191791
TACATGG284000.020.349562
ACATGGG288700.019.317223
CTTATAC65950.018.5903241
GCAGAGT330150.018.3925559
GTGGTAT57450.018.0355341
CATGGGG218350.017.9032884
AGAGTAC282650.017.65718510-11
TATACAC85250.017.4802993
GAGTACT161100.017.23225212-13
GTACTAG21350.017.0149271
Produced by FastQC (version 0.11.5)