##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2138798_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9361189
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32921640616379	33.0	31.0	34.0	31.0	34.0
2	32.49680163492052	34.0	31.0	34.0	31.0	34.0
3	32.615033090347815	34.0	31.0	34.0	31.0	34.0
4	36.14069014096393	37.0	35.0	37.0	35.0	37.0
5	35.9733347975348	37.0	35.0	37.0	35.0	37.0
6	36.01777103314547	37.0	35.0	37.0	35.0	37.0
7	35.98921654076208	37.0	35.0	37.0	35.0	37.0
8	35.97544510638552	37.0	35.0	37.0	35.0	37.0
9	37.81022100931837	39.0	38.0	39.0	35.0	39.0
10-11	37.68721868557509	39.0	37.0	39.0	35.0	39.0
12-13	37.70642212223255	39.0	37.0	39.0	35.0	39.0
14-15	39.02040451271735	40.0	38.0	41.0	36.0	41.0
16-17	38.97015582101804	40.0	38.0	41.0	36.0	41.0
18-19	39.024593670739904	40.0	38.5	41.0	36.0	41.0
20-21	39.017176890562894	40.0	39.0	41.0	36.0	41.0
22-23	38.97957327173401	40.0	38.5	41.0	36.0	41.0
24-25	38.95998545124861	40.0	38.0	41.0	35.0	41.0
26-27	38.86083597778989	40.0	38.0	41.0	35.0	41.0
28-29	38.7809952893232	40.0	38.0	41.0	35.0	41.0
30-31	38.639103267637104	40.0	38.0	41.0	35.0	41.0
32-33	38.55067093881913	40.0	38.0	41.0	34.5	41.0
34-35	38.502438901929764	40.0	38.0	41.0	34.0	41.0
36-37	38.40288823425074	40.0	38.0	41.0	34.0	41.0
38-39	38.3603744984745	40.0	38.0	41.0	34.0	41.0
40-41	38.35536221160329	40.0	38.0	41.0	34.0	41.0
42-43	38.3133432184209	40.0	38.0	41.0	34.0	41.0
44-45	38.19579955470911	40.0	38.0	41.0	33.5	41.0
46-47	38.125188034687355	40.0	37.5	41.0	33.0	41.0
48-49	38.09002051192641	40.0	37.0	41.0	33.0	41.0
50-51	37.990446289644154	40.0	37.0	41.0	33.0	41.0
52-53	37.83913394683212	40.0	37.0	41.0	33.0	41.0
54-55	37.63712042818841	39.0	36.0	41.0	33.0	41.0
56-57	37.48249945951708	39.0	36.0	41.0	33.0	41.0
58-59	37.23171279677754	39.0	35.5	40.0	32.0	41.0
60-61	37.03311633848994	39.0	35.0	40.0	32.0	41.0
62-63	36.78739956065125	38.0	35.0	40.0	31.0	41.0
64-65	36.43884221284738	38.0	35.0	40.0	31.0	41.0
66-67	36.34626155367174	37.0	35.0	40.0	31.0	41.0
68-69	36.09450415031769	37.0	35.0	39.5	31.0	41.0
70-71	35.77075229671395	36.0	34.0	39.0	31.0	41.0
72-73	35.455028930411615	36.0	34.0	39.0	31.0	40.0
74-75	35.05728970563433	35.0	34.0	38.0	30.0	40.0
76-77	33.05068587653851	34.0	31.0	35.5	27.5	38.5
78-79	34.25596099895236	35.0	33.5	37.0	29.5	39.0
80-81	34.294807901143464	35.0	34.0	36.0	30.0	39.0
82-83	34.042568532097576	35.0	34.0	36.0	30.0	37.0
84-85	33.82256657950411	35.0	33.5	35.5	30.0	37.0
86-87	33.64999074870208	35.0	33.0	35.0	30.0	37.0
88-89	33.446471005870684	35.0	33.0	35.0	29.0	36.0
90-91	33.308274211099935	35.0	33.0	35.0	29.0	36.0
92-93	33.267311351941295	35.0	33.0	35.0	29.0	36.0
94-95	33.26224647449748	35.0	33.0	35.0	29.0	35.5
96-97	33.31069486108849	35.0	33.0	35.0	30.0	35.0
98-99	33.350331477730634	35.0	33.0	35.0	30.0	35.0
100-101	32.086440578632335	33.5	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	11.0
20	53.0
21	196.0
22	840.0
23	2982.0
24	7691.0
25	16595.0
26	31147.0
27	50745.0
28	75501.0
29	104167.0
30	138981.0
31	181593.0
32	239359.0
33	327035.0
34	481732.0
35	748241.0
36	1334400.0
37	2303479.0
38	2378412.0
39	925692.0
40	12337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.861669495189126	20.11461364576658	13.780781479788518	24.242935379255776
2	14.965260247420451	20.851019779833788	41.30337475239687	22.88034522034889
3	18.588289686432827	25.90944692466252	29.997786604771363	25.50447678413329
4	13.069456484408759	17.576791827103087	37.76642593859645	31.587325749891708
5	13.629184086115604	37.554063673996794	35.19554791359725	13.621204326290352
6	30.81894831949232	37.11112979344824	18.3642804348892	13.70564145217023
7	27.023639838913628	32.150702223830756	22.930270930327335	17.89538700692829
8	24.70121990366781	36.924751438098035	20.843776762873873	17.53025189536028
9	25.855289870949004	16.7019595707081	20.92331706366599	36.5194334946769
10-11	24.89692410185912	26.36413159039863	28.359797843958745	20.37914646378351
12-13	25.474066338014502	23.784479354516137	28.664081126240344	22.07737318122902
14-15	22.597461850063162	25.122381629213	25.98340477814329	26.296751742580547
16-17	22.42471776137498	28.296679495945952	26.65158053950486	22.627022203174207
18-19	22.536433274471506	27.554086748707174	28.1214826514817	21.78799732533962
20-21	23.150664685437704	26.764068010229618	27.92240218616747	22.1628651181652
22-23	23.021501721452893	26.708884743933247	27.80573637430413	22.463877160309732
24-25	22.762380252934868	26.87595676095042	27.851613823629517	22.510049162485192
26-27	22.63853298555106	26.981292849272602	27.855267138065347	22.524907027110995
28-29	22.59027185606575	26.995915044043272	27.876603944368423	22.537209155522557
30-31	22.83780563346422	26.855938594615125	27.660493356433253	22.6457624154874
32-33	22.717218483332587	27.059305727364535	27.716858511143194	22.506617278159677
34-35	22.798037613487853	27.14622258519525	27.678344690396578	22.377395110920315
36-37	22.793872027223852	27.00936732086674	27.581095556707165	22.615665095202246
38-39	22.680576818097215	27.07574747268488	27.733712135586412	22.509963573631495
40-41	22.80924738613518	26.872251103483187	27.67578331096417	22.642718199417473
42-43	22.730183636598337	26.984895543915066	27.495731084884568	22.789189734602033
44-45	22.70859708127688	27.129648798189592	27.40581712223296	22.75593699830056
46-47	22.98017700474048	27.06299669464397	27.114168697474	22.84265760314155
48-49	22.850043518869263	27.167578008657507	27.031776003608744	22.95060246886449
50-51	22.78346096738566	27.277156796056147	27.062526788522444	22.87685544803575
52-53	22.96758947696216	27.164089290970857	26.9784825097184	22.889838722348582
54-55	22.903316700060213	27.047019136388435	26.956608647288384	23.09305551626297
56-57	22.987840910714	27.206634070385014	26.90703862288981	22.898486396011172
58-59	22.888602281569366	27.305121132509168	26.93256974248171	22.873706843439752
60-61	23.013115007563798	27.31507970868554	26.84187550403082	22.82992977971984
62-63	22.95124299046342	27.43932501165966	26.760555329603292	22.848876668273633
64-65	22.991254427637934	27.311862302677536	26.7485756505011	22.948307619183435
66-67	23.03511880667998	27.236254785565084	26.693687902918274	23.03493850483666
68-69	22.92605277731864	27.240694075659555	26.791381014487243	23.041872132534564
70-71	22.990591817324894	27.069234195300456	26.8114758564218	23.128698130952845
72-73	23.03818497761903	27.072085349139126	26.75849050031284	23.131239172929007
74-75	22.81547193762294	27.24541347948185	26.83405871212995	23.10505587076526
76-77	23.08213738876027	27.152454580079997	26.681374144591878	23.084033886567855
78-79	22.932528022506972	27.164043700471098	26.71249388529918	23.190934391722752
80-81	22.979768016413775	27.257002003864866	26.693076639484246	23.070153340237113
82-83	22.98474260096286	27.12389828095213	26.837150492801452	23.054208625283557
84-85	23.036266208233506	27.191585986552973	26.704464468962723	23.067683336250795
86-87	22.90715365066535	27.171432906919634	26.837850300371525	23.08356314204349
88-89	22.972247549139883	27.08361109536348	26.76342756739319	23.18071378810344
90-91	23.004926396419197	27.107269808312278	26.77792198610482	23.109881809163706
92-93	22.987690180597287	27.127357460470147	26.8028470736495	23.082105285283067
94-95	22.895119044893022	27.114343357657017	26.84206223247073	23.148475364979227
96-97	22.973544065519352	27.144484734102864	26.775202926461215	23.106768273916565
98-99	23.14074205145508	27.771633804304642	26.84802218625732	22.239601957982956
100-101	27.61476719219355	34.634735507938494	27.402116077663923	10.348381222204036
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2255.0
1	1996.5
2	2621.0
3	3678.0
4	4989.5
5	7959.0
6	10392.5
7	12173.5
8	13220.0
9	12688.0
10	11175.0
11	9675.0
12	10178.0
13	13201.5
14	20372.0
15	30028.5
16	37379.0
17	41194.0
18	42067.0
19	40451.0
20	37846.5
21	35858.0
22	36237.0
23	40720.0
24	49329.5
25	61795.5
26	76761.5
27	92580.5
28	110631.0
29	130638.5
30	148753.5
31	165773.0
32	183570.0
33	201510.5
34	217591.5
35	233326.0
36	252489.5
37	272621.5
38	290842.5
39	304839.5
40	317348.5
41	331763.5
42	344121.0
43	356649.5
44	371640.5
45	387320.5
46	400452.5
47	411082.5
48	422091.0
49	425636.5
50	423581.5
51	421659.0
52	413151.0
53	398719.0
54	381786.5
55	359889.5
56	334258.5
57	304458.5
58	272954.5
59	240144.0
60	203664.0
61	168502.5
62	136437.0
63	107536.0
64	81424.0
65	60481.0
66	46144.0
67	35607.5
68	26954.0
69	19417.0
70	13774.5
71	9869.5
72	6880.5
73	4675.0
74	3132.5
75	2135.0
76	1495.5
77	1020.5
78	684.0
79	479.0
80	349.0
81	256.0
82	195.0
83	138.0
84	90.5
85	60.0
86	43.5
87	37.5
88	29.0
89	23.0
90	19.0
91	12.5
92	9.0
93	5.5
94	2.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004732304838626803
3	5.3412018494659176E-5
4	4.272961479572734E-5
5	5.3412018494659176E-5
6	0.0
7	0.0
8	4.272961479572734E-4
9	6.409442219359101E-5
10-11	4.8070816645193254E-5
12-13	2.7240129432276177E-4
14-15	5.341201849465917E-6
16-17	5.341201849465917E-4
18-19	5.341201849465917E-6
20-21	4.2731114303006657E-5
22-23	0.0
24-25	0.0
26-27	1.0156141589870199E-4
28-29	2.1398402737626052E-5
30-31	6.966626003006582E-5
32-33	4.570201084546354E-4
34-35	0.0
36-37	3.963758218350409E-4
38-39	0.001259445774863335
40-41	0.0
42-43	0.0
44-45	3.4982891391269026E-4
46-47	0.0
48-49	5.783238105570814E-6
50-51	1.1117681595918616E-4
52-53	5.205836405171194E-4
54-55	0.0034509825312101596
56-57	0.003697750587509628
58-59	0.004478847219475278
60-61	9.989001550901068E-4
62-63	0.0
64-65	6.739202097544864E-4
66-67	0.0
68-69	0.0
70-71	6.612069923697374E-6
72-73	1.5407409074682594E-4
74-75	4.070332634366768E-5
76-77	0.0
78-79	0.0
80-81	5.57625987121522E-4
82-83	0.0
84-85	0.0
86-87	7.358980410320558E-6
88-89	0.0
90-91	0.0011770420008073749
92-93	1.7000562873181673E-4
94-95	7.868941210353323E-6
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	198.0
20-21	684.0
22-23	1574.0
24-25	3653.0
26-27	6012.0
28-29	11863.0
30-31	29724.0
32-33	35182.0
34-35	47015.0
36-37	72457.0
38-39	86473.0
40-41	84941.0
42-43	93715.0
44-45	108288.0
46-47	107624.0
48-49	102779.0
50-51	94452.0
52-53	90545.0
54-55	97218.0
56-57	100794.0
58-59	101605.0
60-61	100307.0
62-63	95769.0
64-65	97674.0
66-67	103173.0
68-69	100384.0
70-71	99504.0
72-73	93917.0
74-75	94641.0
76-77	91946.0
78-79	98594.0
80-81	99444.0
82-83	95950.0
84-85	94477.0
86-87	98437.0
88-89	105765.0
90-91	116183.0
92-93	113324.0
94-95	127906.0
96-97	194363.0
98-99	766225.0
100-101	5296410.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	82.26659954271624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.67632836939208	72.95099994883185
2	7.357704622983249	12.105866795451098
3	2.0460143602578014	5.0495593210192595
4	0.8076515996940468	2.657710028882572
5	0.4084086990558591	1.6799197447495033
6	0.22061932560946823	1.0889761026778946
7	0.13476957249413593	0.7760924115642702
8	0.08860760063527676	0.5831556798322594
9	0.061295169757079424	0.45382906658776173
>10	0.19433464744048487	2.369541274152394
>50	0.0034716737433918093	0.1928907349777031
>100	7.943589371324542E-4	0.09145889127355414
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.20472110967955E-5	0.0	0.0	0.0
3	0.0	4.272961479572734E-5	0.0	0.0	0.0
4	0.0	1.0682403698931834E-4	0.0	0.0	0.0
5	1.0682403698931835E-5	1.3887124808611385E-4	0.0	0.0	0.0
6	2.136480739786367E-5	1.3887124808611385E-4	0.0	0.0	0.0
7	2.136480739786367E-5	1.3887124808611385E-4	0.0	0.0	0.0
8	3.20472110967955E-5	1.7091845918290935E-4	0.0	0.0	1.0682403698931835E-5
9	4.272961479572734E-5	2.1364807397863668E-4	0.0	0.0	1.0682403698931835E-5
10-11	5.341201849465917E-5	2.2433047767756852E-4	0.0	0.0	1.0682403698931835E-5
12-13	6.4094422193591E-5	2.5103648692489813E-4	0.0	0.0	1.0682403698931835E-5
14-15	7.477682589252283E-5	4.005901387099438E-4	0.0	1.0682403698931835E-5	4.272961479572734E-5
16-17	1.0148283513985243E-4	4.379785516562052E-4	0.0	1.6023605548397754E-5	5.8753220344125085E-5
18-19	1.1750644068825018E-4	4.967317720003302E-4	0.0	2.136480739786367E-5	7.477682589252285E-5
20-21	1.1750644068825018E-4	5.661673960433872E-4	0.0	3.20472110967955E-5	7.477682589252285E-5
22-23	1.1750644068825018E-4	6.142382126885805E-4	0.0	3.7388412946261416E-5	8.545922959145468E-5
24-25	1.816008628818412E-4	7.15721047828433E-4	0.0	6.943562404305692E-5	9.614163329038651E-5
26-27	1.9762446843023894E-4	7.584506626241602E-4	0.0	1.602360554839775E-4	9.614163329038651E-5
28-29	2.0296567027970486E-4	7.637918644736261E-4	0.0	6.623090293337737E-4	1.0148283513985242E-4
30-31	2.189892758281026E-4	8.545922959145467E-4	0.0	0.0021578455471842305	1.228476425377161E-4
32-33	2.2433047767756852E-4	8.545922959145467E-4	0.0	0.005335860647616451	1.28188844387182E-4
34-35	2.456952850754322E-4	9.881223421511947E-4	0.0	0.009331079631016957	1.28188844387182E-4
36-37	2.6706009247329585E-4	0.0011109699846889108	0.0	0.01568176863003193	1.28188844387182E-4
38-39	2.6706009247329585E-4	0.0011590408013341042	0.0	0.02488465941666171	1.28188844387182E-4
40-41	2.777424961722277E-4	0.0011964292142803655	0.0	0.03408755020329148	1.3887124808611385E-4
42-43	2.777424961722277E-4	0.0012445000309255587	0.0	0.041335561113016735	1.3887124808611385E-4
44-45	2.937661017206254E-4	0.0013726888753127406	0.0	0.050148544164635496	1.3887124808611385E-4
46-47	3.0978970726902317E-4	0.0014421244993557977	0.0	0.060109885613889434	1.495536517850457E-4
48-49	3.0978970726902317E-4	0.0014421244993557977	0.0	0.06925936438202454	1.7091845918290935E-4
50-51	3.0978970726902317E-4	0.0015115601233988544	0.0	0.07816314786508423	1.7091845918290935E-4
52-53	3.151309091184891E-4	0.0015169013252483204	0.0	0.086976130916703	1.9228326658077302E-4
54-55	3.20472110967955E-4	0.0016077017566892412	0.0	0.09504668691124599	2.1364807397863668E-4
56-57	3.20472110967955E-4	0.0016557725733344344	0.0	0.10348578583340215	2.189892758281026E-4
58-59	3.20472110967955E-4	0.0016664549770333663	0.0	0.11176464870007433	2.2433047767756852E-4
60-61	3.418369183658187E-4	0.0017091845918290934	0.0	0.1195254149873483	2.2433047767756852E-4
62-63	3.418369183658187E-4	0.001741231802925889	0.0	0.12697639156735324	2.2433047767756852E-4
64-65	3.418369183658187E-4	0.001933515069506662	0.0	0.13461431021208953	2.2433047767756852E-4
66-67	3.5251932206475055E-4	0.001986927088001321	0.0	0.14189436833291155	2.2967167952703444E-4
68-69	3.8456653316154603E-4	0.0020029506935497193	0.0	0.14941478053695956	2.3501288137650037E-4
70-71	3.8990773501101195E-4	0.002013633097248651	0.0	0.1569618987502549	2.3501288137650037E-4
72-73	3.952489368604779E-4	0.0020243155009475825	0.0	0.162981433234603	2.56377688774364E-4
74-75	3.952489368604779E-4	0.0020563627120443783	0.0	0.16787931533056324	2.56377688774364E-4
76-77	3.952489368604779E-4	0.0021044335286895715	0.0	0.17197067594725413	2.6706009247329585E-4
78-79	4.059313405594097E-4	0.002147163143485299	0.0	0.17560803440674044	2.777424961722277E-4
80-81	4.1127254240887564E-4	0.0021738691527326283	0.0	0.17706084130979516	2.830836980216936E-4
82-83	4.2729614795727336E-4	0.0021898927582810262	0.0	0.17777122115577412	2.8842489987115954E-4
84-85	4.379785516562052E-4	0.0022165987675283557	0.0	0.17858308383689295	2.8842489987115954E-4
86-87	4.379785516562052E-4	0.002248645978625151	0.0	0.1789035559478609	2.991073035700914E-4
88-89	4.4866095535513704E-4	0.002382176024861799	0.0	0.1789943563793018	2.991073035700914E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10410	0.0	42.35121	1
GTATCAA	18400	0.0	34.056442	1
TCAACGC	21995	0.0	27.738726	4
ATCAACG	22350	0.0	27.279665	3
CAACGCA	22350	0.0	27.242725	5
AACGCAG	22890	0.0	26.672459	6
TATCAAC	23450	0.0	26.440664	2
ACGCAGA	26700	0.0	22.789085	7
CGCAGAG	27250	0.0	22.344269	8
GTACATG	27305	0.0	21.619179	1
TACATGG	28400	0.0	20.34956	2
ACATGGG	28870	0.0	19.31722	3
CTTATAC	6595	0.0	18.590324	1
GCAGAGT	33015	0.0	18.392555	9
GTGGTAT	5745	0.0	18.035534	1
CATGGGG	21835	0.0	17.903288	4
AGAGTAC	28265	0.0	17.657185	10-11
TATACAC	8525	0.0	17.480299	3
GAGTACT	16110	0.0	17.232252	12-13
GTACTAG	2135	0.0	17.014927	1
>>END_MODULE