FastQCFastQC Report
Fri 27 May 2016
SRR2138797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2138797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8491924
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83050.042.7426531
GTATCAA143900.034.79171
TCAACGC166300.029.0471464
ATCAACG168150.028.8271433
CAACGCA170750.028.265625
AACGCAG177100.027.2286686
TATCAAC181150.026.9904252
ACGCAGA207200.023.2933527
CGCAGAG209950.022.9683118
GTACATG220450.021.115431
TACATGG219050.020.390432
ACATGGG223400.019.7303643
GCAGAGT260450.018.4023639
CATGGGG164100.017.6262264
GAGTACT136550.017.39663712-13
AGAGTAC226900.017.17543210-11
CAGAGTA252700.016.3246510-11
GTGGTAT48850.016.2814941
TATACCG6550.015.9767565
GTACTTT158250.015.17028114-15
Produced by FastQC (version 0.11.5)